Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,906,8530AT24.3% 197.3 / 33.7 111intergenic (+24/‑11)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (66/18);  new base T (27/0);  total (93/18)
Fisher's exact test for biased strand distribution p-value = 5.82e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTTTTCATCCCTGATTCA  >  DH1/3906704‑3907002
                                                                                                                                                       |                                                                                                                                                       
cccTCGCACCAAAAACCACGTTGAAATTGCTCGCTCTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTaa                                                                                                                                                        <  3:531226/151‑1 (MQ=255)
   gcccACCAACCACCACGTTTATATAGCTCCCCGTTGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CCCCAAACGAGGCGATATCAAAAAAAGTAAGAt                                                                                                                                                     <  5:906139/148‑1 (MQ=18)
    cgcacctaccaactcGTTGATAGAGCTCGCAGTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATg                                                                                                                                                    <  5:537852/137‑1 (MQ=18)
    cGCACCAAAAACCTCGTTGAAATTCCTCTCTGTTGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATg                                                                                                                                                    <  4:463157/151‑1 (MQ=18)
    cGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAATTGAGGCTAGTTCAAATAAAGTTTGATg                                                                                                                                                    >  1:1117189/1‑151 (MQ=18)
     gCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTACCCCCCCTC‑G‑CACCAAAAGACGGTAAATCAAAAAAAGTAAgcaa                                                                                                                                                    >  4:819344/1‑147 (MQ=18)
       acaaaacaCCACTTTGATATTCCGCGCAGTGGTCGAATGTGGAGGACTTGGTAGACCCGATAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACt                                                                                                                                                 <  7:585702/148‑1 (MQ=18)
       aCCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATAAAAAAAAGTAAGATGACt                                                                                                                                                 >  2:1011722/1‑151 (MQ=255)
       aCCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAAGAGCCGAAGACCAAAAATGTAACAtcc                                                                                                                                                   >  7:1647517/1‑147 (MQ=18)
        ccAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAACTCGGGATATCAAAAAAAGTTAGATGAAtg                                                                                                                                                >  3:294041/1‑151 (MQ=18)
         cAAAAACCACGTTGATATTGCTCGCACTGTTGTTAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTGTTACGGTCGTGGGGGGTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtgt                                                                                                                                               <  1:504150/151‑1 (MQ=21)
          aaacacgtcGTTTATATTGCTCGGTCTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGGCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtgtg                                                                                                                                              <  1:1164885/143‑1 (MQ=18)
          aaaaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtgtg                                                                                                                                              >  1:954061/1‑151 (MQ=255)
           aaaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAAAAGGTTAAATCAAATAAAGTATAATGCCtgtgt                                                                                                                                             >  7:1386430/1‑150 (MQ=18)
           aaaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGC‑G‑CACCAAACGAGGAGATATCAATTAATGTTACATGAGtgtgt                                                                                                                                             >  1:1317786/1‑150 (MQ=18)
            aaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAATAAGGGGATATCAATTAATGTTTGTTGACtgtttt                                                                                                                                            >  1:1097415/1‑148 (MQ=17)
            aaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAGGAGGGGATATCAAATATATTTAGATGCAtgtggt                                                                                                                                            >  8:598291/1‑149 (MQ=18)
            aaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTACCCCCCCAC‑G‑CACCAAAACAAGGGAAAACAAAAAAAGGTAGTAGACtgtggt                                                                                                                                            >  2:1368977/1‑149 (MQ=17)
                 aCGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGt                                                                                                                                       <  1:1010336/151‑1 (MQ=255)
                  cGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTg                                                                                                                                      <  2:954527/151‑1 (MQ=255)
                   gTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CCCCAAAGGAGGTTAGATCCATTAAAGTTTGATGCCtgttggttggtcg                                                                                                                                     >  3:749096/1‑141 (MQ=17)
                   gTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAGGACGTTAGATCAAAAAATGTTAGATgcgtgtttgttgctgg                                                                                                                                     >  3:741187/1‑136 (MQ=17)
                   gTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAAGAGTCGAGATCATAAAAAGTTTGATGCCtgttgtttgctgg                                                                                                                                     >  3:803592/1‑141 (MQ=17)
                   gTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAAGAGGTTATTTCAAATAAAGTTTGTTGACtgatttatcatgc                                                                                                                                     >  1:1473948/1‑140 (MQ=17)
                     tGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAAGACGGGATATCAAATATTGTTAGATGCGtgtgttttggtggcg                                                                                                                                   >  3:369037/1‑140 (MQ=17)
                       taattagtcattgtGGGCGAAGGTGTCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTATTACTGACGTGGGGGTGCAAGTCCCCCCCCTC‑G‑CACGAAACGAGTCGATATCTATAAAAGTAAGATGAGTGTGCGAAGGTGGCgg                                                                                                                                  <  6:1189678/137‑1 (MQ=18)
                        tatTGCTCGCACTGGGCGAAGGTAGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAACAGGGGAATTCAATAAAAGTTTCATGACtgtgttatgttgccggat                                                                                                                                >  3:878639/1‑137 (MQ=14)
                        aatttgttcctctctgCGAAGGGTTCGTAATTGGTAGAGGAGCTAGCTTTAGGTGTTAGTGTTATTACGGACGTGGTGGTTCAAGTCCCCCCCCTC‑G‑CACTAAACGATCCGATATCAATAAAAGTAATATGTCTGTGCGAAGGTGGCGGaa                                                                                                                                <  4:403560/136‑1 (MQ=12)
                         atTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGC‑G‑CACCAAAAGAGGGGAGATCAAAAAATGTTAGTTGCCTGTTGGTTGGTGGCGGatt                                                                                                                               >  7:111141/1‑149 (MQ=17)
                           tGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCCAAATACGGGATATCAAAAAAAGTTAGATGAGTGTGGGTTGGTGGCGGAAtgg                                                                                                                             >  3:1634210/1‑149 (MQ=17)
                           tGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAAGAGGCTACATCAAAAAATGTAAGATGAGTGTTGGTTGCTGGCGGAATTg                                                                                                                             >  5:498256/1‑151 (MQ=17)
                               ggccgtgcgCGACGGGGGCGGAATTGGTAGACGCGCTAGCGGCCGGTGTTAGGGTTCGTCGGGACGTGGGGGTTCAAGTCCCCCCCCTC‑C‑CCCCACCCGCGGCGATTTCAAAAAAAGTAAGCTGACTGTGCGAAGGTGGCGGAATTGGTa                                                                                                                          <  3:1375130/142‑1 (MQ=18)
                                gCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTACCCCCCCTC‑G‑CACCAAACGACGGTAAATCAAAAAAAGTTAGATGAATGTTATTAGGTGGCGGAAttaattt                                                                                                                         >  7:436661/1‑145 (MQ=17)
                                     gggCGAAGGGGGCGGACCCGGCAGCCGCGCTAGCTTCAGTTTTTAGTGTGCTTACGGAGGGTGCGGTTCAAGGCGCAGGGCTCGG‑CAGTTCA‑GACGCGATATCAAAAAATGTAATCTGACTGTGCGAAGGTGGCGGAATTGGTAGAcgc                                                                                                                     <  7:1494547/149‑1 (MQ=255)
                                        cGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CCCCAAAAAAGGTTAAATAAAAAAAAAGAAGAAGCCTGTGCCAACGAGGCCCCATTAGGAGAcgcggttg                                                                                                                >  5:931883/1‑147 (MQ=2)
                                             gTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAAGAGGGGATATCAAAAAAAGTAAGATGCCTGTTGTTTGGTGGCGGATTTGGTataatagctatcatc                                                                                                            >  1:559801/1‑136 (MQ=17)
                                             gTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGGTCAATTACCCCCCCAC‑GCCACCAAA‑TAGGGGAAATCAAAAAAAGTAAGATGAATTTGAGAAGGTTGGGGAATTGTTataaacgaaaaaatc                                                                                                            >  8:1443377/1‑136 (MQ=12)
                                                          tAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTACCCCCCCTC‑G‑CACCAAAAAAGGTTAAATCAAAAAATGTTTGATGATTGTTTTTTGGTGCCGGAATTAGTATAATTGATATCTGCAGGTTTTAgtgaa                                                                                               >  2:779348/1‑148 (MQ=2)
                                                                cgcTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGGTGAAATCCCCCCCCTC‑G‑CACCAAAAGAGGCGATATCAAAAAAAATTACAAGCCTGTTCGAAGTTGGCGGAATTGGTAGACGCGCTACGGTGAGGTGTTACTGTGCTTAAgg                                                                                        >  5:927654/1‑151 (MQ=18)
                                                                  cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGt                                                                                                                                       <  1:149179/102‑1 (MQ=255)
                                                                   tAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAATAGGGGATTTCAAAAAAAGTTTGATGCCTGTGGGTAGCTGGCGGAATTGGTTTCCGGGCTAGCATCAGGTTTTACTGTCGTTACGGACg                                                                                     >  3:1340957/1‑151 (MQ=18)
                                                                          aGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CCCCAAAAGAGGCGATATCAAAAAATGTTACTTGAATGTGATTTCGTGGCGGATTTTGTATAAGAGCTATCATCAGGTGTTACTCACCATACGGACGGggcggg                                                                              >  1:1557/1‑150 (MQ=12)
                                                                          aGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAGGAGGGGATATCAATAAAAGTTAGATGCCTGTGGGATGGTGGCGGAATTGGTATACGCTGAATCATCAGGTTTTAGTGTACTTACGGCAGTGGGGGt                                                                              >  5:455288/1‑151 (MQ=21)
                                                                            gtgtTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCTAAATCAAAAAAAGTAACATGACTGTGCGTACCTGGCTGAATTGGTTGACGCGGGAGCTTCAGGGGGGAGTCTCATTTCGCCCGAGGGGGttt                                                                            >  6:311169/1‑150 (MQ=16)
                                                                             tgtTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAAGAGGGGATATCAAAAAATGTTAGTTGCCTGTTGTATGGTGGCGGAATTGGTATAAGGTATATCTTCAGGATTTAGTGTCATTACGGACGGGGGGGttg                                                                            >  3:37827/1‑149 (MQ=16)
                                                                              gtTAGTGTTCTTACGGACGTGGGGGTTGAAGTGCCCCCCCGC‑G‑CCCCCAAAGAGGGGAGATGAAAAAAAGTTAGATGAAGGGTCGAAGGGGGGGGGATTGGGAGACCCCCTAGCGTGAGGGGTTAGTGGGCGTTCGGGCGTtgggggtaac                                                                          >  5:360893/1‑146 (MQ=255)
                                                                              gtTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAACAGGGGATATCAAAAATAGTTTGTTGAGTGTGAGTTGGTGGCGGAATTGATATAAGGGGTATCGGCAGGTGTTAGTGTAATAAGGGCCGGGGGGGttggt                                                                          >  5:918941/1‑148 (MQ=12)
                                                                                   tgtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCAC‑G‑CACCAAAAGAGGCGATATCAAAAAAAGTAAGATGACTGTGGGAAGGTGGCGGAATTGGTAGACGGGGTAGCGTCAGGTGGTAGTGTAATTAAGGACGTggggggtggactaac                                                                     >  8:946467/1‑141 (MQ=25)
                                                                                      tCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAAAGAGGGGATATCAAAAAAAGTTAGATGACTGTGCGTAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTT‑GAAGT‑cacccc                                                                >  5:232448/1‑151 (MQ=25)
                                                                                            ggACGTGGGGGTTCAAGTCCCCCCCCGC‑G‑CACCAAACGAGGTTATATCAAAAAAAGTATCATGCGTGAGCTAAGGTGGCGGAATTGGGATACGGGCAAGCATCAGGTTTTACTGACCTTAAGGCCGGGGCGGGT‑GAAggaaacccccaagc                                                           >  7:1551656/1‑138 (MQ=255)
                                                                                             gACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑GAAGG‑CCCCCCCCTCGcc                                                         >  3:538419/1‑150 (MQ=255)
                                                                                             gACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGAGATCAAAAAAAGTATGATGCCTGTGGGAAGGTGGCGGATTTGGTTTCCTCGCTAGCTTCAGGTGTTAGTGTCGTTTCGGCCGTGGGGGTT‑GTAGG‑CCCCCGCCACGcc                                                         >  7:983538/1‑150 (MQ=21)
                                                                                                             tCCCCCCCCTC‑G‑CACCAAAGGAGGCGATATCAAAAAAAGTAACATGAATGTGAGAAGGTGGCGGATTAGGTTGACGAGCTATCTTCAGGATTTAGTGAAATTAAGGACGTGGGGATT‑CAAGA‑ACACACCATGGCACCAAGTATAttttcgt                                         >  2:1200390/1‑147 (MQ=255)
                                                                                                             tCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CCAGT‑CCCCCCCCGCGCACCAATTATCTTTAcgt                                         >  5:468774/1‑149 (MQ=255)
                                                                                                             tCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGT‑GAAGG‑CCCCCCCCCCCCCCCAATTATCTTTACtt                                         >  1:1372927/1‑151 (MQ=25)
                                                                                                               cccccccTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGCCGTGGGGGTT‑CAAGT‑CCCCCCCCGAGCCCCAAATATATTTTCttgc                                       >  6:511029/1‑149 (MQ=21)
                                                                                                                  ccccTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCttt                                    >  4:639534/1‑151 (MQ=255)
                                                                                                                    ccTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCt                                  >  5:685155/1‑151 (MQ=255)
                                                                                                                     cTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑GAAGT‑CCCCCCCCGCCCCCCAAATATCTTTTCTTGCTTTGtt                                 >  7:1472720/1‑151 (MQ=21)
                                                                                                                       c‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑GAAGT‑CCCCCCCCTAGCACCAATTATCTTTTCTTCCTTTCtttt                               >  8:70251/1‑149 (MQ=255)
                                                                                                                       c‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCGCGCACCAAATAACTTTACTTCCTTTCTTGt                               >  1:1555829/1‑151 (MQ=255)
                                                                                                                       c‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGT‑GAAGT‑CCCCCCCCGCGCCCCAATTATCTTTACTTCCTTTCTTGt                               >  2:1379097/1‑151 (MQ=255)
                                                                                                                        ccccACCAAACGAGTCCATATCTTATAATTTATGATGACTGTGCGAAGGTGTCGTAATTTGTAGACGCGCTTGCTTCAGTTGTTATTGTTCTTACGTACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTg                                <  8:941488/148‑1 (MQ=17)
                                                                                                                         g‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGtt                              >  5:1016014/1‑151 (MQ=255)
                                                                                                                         g‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGtt                              >  3:1004656/1‑151 (MQ=255)
                                                                                                                         g‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGtt                              >  5:1099639/1‑151 (MQ=255)
                                                                                                                         g‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCCCGCACCAATTATCTTTACTTCCGTTCTTGtt                              >  3:275068/1‑151 (MQ=255)
                                                                                                                           cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGttt                             >  2:376749/1‑151 (MQ=255)
                                                                                                                           cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGT‑CCAGT‑CCCCCCCCGCGCACCAATTATGTTTACTTCCGTTCTTGttt                             >  5:1190096/1‑151 (MQ=255)
                                                                                                                            cccAAACGATGCTACATCAAAATACGCACTATGCCTCTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttc                            <  8:657487/150‑1 (MQ=17)
                                                                                                                            cccAAACGAGGCGATATCAAAATATTTAATATTAATGTGCGAAGGTGGCTGAATTCCAATACGCGCTAGCTTCTTGTCTTAGACGACTTTCTTACGTGGTGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttc                            <  8:1101530/150‑1 (MQ=16)
                                                                                                                              cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑GAAGT‑ACCCCCCCGAGGCCCAATTATCTTTACTTACGTTGTTGTttctt                          >  8:1164916/1‑151 (MQ=21)
                                                                                                                               aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttcttc                         >  4:1221391/1‑151 (MQ=255)
                                                                                                                               aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttcttc                         >  1:663602/1‑151 (MQ=255)
                                                                                                                               aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttcttc                         >  3:911207/1‑151 (MQ=255)
                                                                                                                                 aCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑GCCCCCCCTCGCCCCAATTATCTTTACTTGCTTTGTTGTTTCTTCCt                       >  6:1188591/1‑151 (MQ=255)
                                                                                                                                 aCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCt                       >  5:1109118/1‑151 (MQ=255)
                                                                                                                                 aCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCACCCACCAATTATCTTTAATTCCTTTCTTGGttattgc                        >  8:583822/1‑148 (MQ=21)
                                                                                                                                   gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCCAGGC‑CCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGTTTGTTGCtt                      >  3:1199515/1‑151 (MQ=21)
                                                                                                                                   gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑GCCCCCCCGCGCCCCCATTATGTTTACTTCCTTTCTTGTTTGTTACttt                     >  1:540789/1‑150 (MQ=21)
                                                                                                                                   gAGGCGATATCAAAAAAAGTAAGATGACTCTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCCCCAATTATCTTTACTACCGTTCTTGTTTCTTCCTTg                     >  6:508349/1‑151 (MQ=255)
                                                                                                                                    aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATGTTTACTTCCTTTCTTGTTTGTTCCTTg                     >  1:696721/1‑150 (MQ=255)
                                                                                                                                    aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACGTCCTTTCTTGTTTGTGCCTTgg                    >  8:544003/1‑150 (MQ=255)
                                                                                                                                    aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGT‑CCAGT‑CCCCCCCCGCGCCCCAAGTAAGATTTCGTCCTTTCGTGTTTGTTCCttt                     >  8:1250888/1‑149 (MQ=18)
                                                                                                                                      gCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑GCCCCCCCTCGCCCCAATTATGTTTACTTCCTTTGTTGTTTCTTCCTTGAtt                  >  6:777135/1‑151 (MQ=255)
                                                                                                                                         atatCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCTCTCGCACCAATTATCTTTACTTCCATTCTTGTTTCTTCCTTTAttttt               >  3:1184080/1‑151 (MQ=255)
                                                                                                                                         atatCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGAttttt               >  5:779794/1‑151 (MQ=255)
                                                                                                                                         atatCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCGCGCACCAATTATCTTTTCTTCCTTTGTTGTTTGTTACTTGAttttt               >  4:803055/1‑151 (MQ=255)
                                                                                                                                             cAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATGTTTACTTCCTTTGTTGTTTGTTCCTTGATTTTTCATc           >  4:1317784/1‑151 (MQ=255)
                                                                                                                                               aaaaaaGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGTCCCCCCCCCTCGCCCCAATTATGTTTACTTCCTTTCTTGTTTCTTCCTTGATTTTTGATcc          >  2:155686/1‑151 (MQ=255)
                                                                                                                                               aaaaaaGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTTTTCATgcc         >  2:1463901/1‑151 (MQ=255)
                                                                                                                                                aaaaaGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTTTTCATCCCt        >  3:169303/1‑151 (MQ=255)
                                                                                                                                                aaaaaGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTTTTCATCCCt        >  7:492411/1‑151 (MQ=255)
                                                                                                                                                aaaaaGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCGTTCTTGTTTGTTCCTTGAtttttgctgcct        >  2:1548359/1‑144 (MQ=255)
                                                                                                                                                aaaaaGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGG‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGGTTCTTCCTTGATTTTTAATgccg        >  8:1318907/1‑150 (MQ=255)
                                                                                                                                                  aaaGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCGCGCCCCAATTATCTTTTCTTCCTTTCTTGTTTCTTCCGTGATTTTTCATcccgg       >  1:93412/1‑148 (MQ=255)
                                                                                                                                                  aaaGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGT‑GAAGT‑CCCCCCCCGCGCACCAATTTTGTTTAAGTGCGTTGTTGTTTGTTCCTTGATTTTTCATCCCTgt      >  1:532251/1‑150 (MQ=18)
                                                                                                                                                   aaGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATATTTACTTCCTTTCTTGTTTGTTCCTTGATTTTTCATCCCTGAt     >  5:1583635/1‑151 (MQ=255)
                                                                                                                                                     gTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑GAAGT‑GCCCCCCCGCGCCCCAATTATGTTTTCTTCCGTTGTTGTTTGTTTCTTTTTTTTTTATGCCTGAttg   >  6:45133/1‑150 (MQ=17)
                                                                                                                                                       aaGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑GAAGT‑CCCCCCCCGCGCCCCAATTATCTTTACTTCCTTTCTTGTTTGTTCCTTGATTTTTCATCCCTGATTCa  >  1:1001220/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
CCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTC‑G‑CACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTT‑CAAGT‑CCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTTTTCATCCCTGATTCA  >  DH1/3906704‑3907002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑