Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA30.9% 220.5 / 34.1 212intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base C (106/17);  new base A (55/0);  total (194/18)
Fisher's exact test for biased strand distribution p-value = 1.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

AAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCA  >  DH1/613632‑613920
                                                                                                                                          |                                                                                                                                                        
aagaaagCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTGTGCTTTTTGAGGGGCAGAAAGATGAATGAGTGTCCGCGACACTATACCCAAAAGAATGCCGATTATCGGTCAGTTTAAACTGTTGTa                                                                                                                                                >  7:807966/1‑149 (MQ=11)
     agCGGCTTATCGGTCAGTTTAACCTGGTTTACGTAAAAACCCGATACGGCGGGTTTATGATTTTTGAGTGGCAGAAAGAAAAAAGAAAGTAACAGACACTATAACCAAAAGAAAGAGGCTAATAGTTAAGTTTTACCTGTTGTACGTaaa                                                                                                                                          >  8:605101/1‑150 (MQ=2)
         gCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACACAAAAGAAACCGGCTGATCTGTCATTTTGACCTGTTATAAGtaataaaa                                                                                                                                      >  5:678320/1‑148 (MQ=11)
           ttATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTTTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTAAGTGTCACCTGTTTTACGTAATAACACG‑c                                                                                                                                  >  1:309317/1‑151 (MQ=255)
            tATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑Ct                                                                                                                                 >  7:952092/1‑151 (MQ=35)
               cGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAAACCG‑CTTcg                                                                                                                              >  8:212382/1‑151 (MQ=255)
                ggTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAACCAAAAGAAAGCGGCTTATCGGTGAGTTTCACCTGTTGTACGTAATAACCCG‑CTTctg                                                                                                                             >  3:1147403/1‑149 (MQ=255)
                  tCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTcggcg                                                                                                                           >  1:790111/1‑151 (MQ=35)
                  tCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAAACCG‑ATTcggcg                                                                                                                           >  6:1534921/1‑151 (MQ=255)
                  tCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCAAAAAGAAAGCGGCTTATAGGTCAGTTTTACCTGTTTTACGTAAAAAACCG‑ATCcggcg                                                                                                                           >  7:125663/1‑151 (MQ=11)
                   cAGTTTCACCTGGTTTACGTAAAAAACCGATTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTACACGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAATCCgaatagcgtg                                                                                                                           >  6:1681593/1‑142 (MQ=11)
                    aGTTTCACCTGGTTTACGTAAAAACCCGCTACGGCGGGTTTATGCTTTTGGAGGGGCAGAAAGATGAATCACTGTCCACGACACTATACCCAAAAGAAACCGGCATATCGCTCAGTTAAACATGATCTACGTAAAAAacccatacggacgg                                                                                                                          >  4:1157123/1‑140 (MQ=11)
                     gTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCG‑ATACGGCGGGt                                                                                                                        >  8:1088891/1‑151 (MQ=255)
                      tttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCG‑GTGCGGCGGGtt                                                                                                                       >  5:962581/1‑151 (MQ=255)
                      tttCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGtt                                                                                                                       >  4:140064/1‑151 (MQ=25)
                           ccTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCG‑GTGCGGCGGGtttgtgc                                                                                                                  >  1:1400417/1‑147 (MQ=255)
                           ccTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTGTACGTAAAAACCCG‑GTGCGGCGGGTTTTAAc                                                                                                                  >  3:1248533/1‑151 (MQ=255)
                           ccTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCG‑GTGCGGCGGGtttgtgc                                                                                                                  >  5:247243/1‑147 (MQ=255)
                            cTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGtttgtgct                                                                                                                 >  3:155608/1‑146 (MQ=21)
                             tGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCG‑ATTCGGCGGGtttaagatt                                                                                                                >  6:1351232/1‑145 (MQ=255)
                              ggTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAACCCG‑GTGCGGCGGGTTTGTCCttt                                                                                                               >  1:1539665/1‑151 (MQ=11)
                                  tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCG‑GTGCGGCGGGtttgtgattgtggt                                                                                                           >  3:7351/1‑140 (MQ=11)
                                  tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCG‑CTGCGGCGGGTTTGTGCTTTTgg                                                                                                            >  7:118922/1‑150 (MQ=9)
                                  tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAACCCG‑CTTCGGCGGGTTTTTCATTTTgg                                                                                                            >  1:954157/1‑150 (MQ=255)
                                    cGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCG‑ATACGGCGGGTTTGTTATTTTTGAgg                                                                                                         >  2:741957/1‑151 (MQ=11)
                                    cGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAAACCG‑ATTCGGCGGGTTTGAACTTTTTGAgt                                                                                                         >  6:1801612/1‑150 (MQ=11)
                                       aaaaaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCG‑CTGCGGCGGGTTTGTCCTTTTGGAgtggg                                                                                                      >  3:1290626/1‑150 (MQ=255)
                                             ccGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCAGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTGTACTTTTGGAGTGGCAGaaat                                                                                                >  1:1822339/1‑150 (MQ=255)
                                                 ttCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGTTTTACGTAAAAAACCG‑ATTCGGCGGGTTTGTGCTTTTTGAgtgcgagttattcat                                                                                            >  1:1107950/1‑137 (MQ=11)
                                                 ttCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAACCCG‑GTGCGGCGGGTTTTTTCTTTTTGAGTGGCAGTTAGa‑tgt                                                                                           >  6:1135453/1‑150 (MQ=11)
                                                   cggcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCG‑ATACGGCGGGTTTAAAATTTTTGAGTGCCATaaataaaaa                                                                                           >  4:765540/1‑144 (MQ=11)
                                                     gcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAATAACCCG‑GTTCGGCGGGTTTGTTCTTGTTGAGGGGGAGTAATa‑tgagtgg                                                                                       >  1:122657/1‑150 (MQ=11)
                                                           ttttGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAACCCG‑ATTCGGCGGGTTTTTACTTTTTGAGGGCCATAAAGA‑TCAATGAcattcc                                                                                 >  2:462320/1‑146 (MQ=11)
                                                                cTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCG‑CTGCGGCGGGTTTTTACTTGTTGAGTGCGAGATAGa‑ggaagccctggccgcgac                                                                            >  8:287241/1‑137 (MQ=11)
                                                                       aGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCG‑ATTCGGCGGGTTTATACTATTGGAGTGGCAGAAAGA‑TGAAAGACTGTCGACGACACtaga                                                                      >  2:584173/1‑148 (MQ=11)
                                                                            cAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCG‑ATACGGCGGGTTTTTACTTTTTGAGTGGGAGAAATA‑AAAATCACTTTCGACGACACtataagaaa                                                                 >  2:1478715/1‑145 (MQ=11)
                                                                              gAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCG‑ATGCGGCGGGTTTATACTTTTTGAGGGCCAGAAAGA‑CAAAACGCAGTCCACGacacagacaccaca                                                                >  4:192855/1‑139 (MQ=11)
                                                                               aaaGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCG‑ATACGGCGGGTTTGTAATTTTTGAGTGGGAGAAAGA‑TAAAACACTTTCGGCGacacaaaaggcaaatgc                                                             >  6:400711/1‑138 (MQ=2)
                                                                                 aGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAAACCG‑CTACGGCGGGTTTTTTATTTTTGAGTGGCATAAATA‑AAAAACCCATTCCGCGACACTATACGAAaatgaa                                                            >  3:897678/1‑150 (MQ=2)
                                                                                   atgaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCG‑ATACGGCGGGTTTAAAATTTTTGAGTGCCATAAATA‑AAAATAACTGAACACCATACTATACCCaaaaaaaatc                                                         >  4:549055/1‑149 (MQ=2)
                                                                                    tgaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCG‑ATTCGGCGGGTTTTAACTTTTGGAGTGCCATAAATA‑GGAGCCACTTTCGACGAGACTATAATCAAaagcaagcc                                                        >  8:331424/1‑150 (MQ=11)
                                                                                     gaatgaCTGTCCACGACACTATACCCAAAAGAAAGCAACATATACGTGAATTTAAACAGTTTTACTTAAAAACAAG‑CTAACGCGGGTATAAGCTTAATTAAAAAAAAAAAGA‑TGAATGACTGTCCACGACACTATACCGAaaagatcgggg                                                       >  6:464728/1‑145 (MQ=2)
                                                                                           cTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCG‑CTGCGGCGGGTTTATCATTGTGGAGTGGGAGTAAGA‑GCAGTCACTGTCCCCGACAAGGTCCCCACAAGACAGCggatgctc                                                 >  5:1764001/1‑145 (MQ=2)
                                                                                           cTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCG‑CTACGGCGGGTTTATACTTTTGGAGTGGCAGAAAGA‑TGAAACACTGGCCACGACACTATACACAAAAGAAAGCGGcatctc                                                 >  1:615219/1‑146 (MQ=11)
                                                                                            tGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑GTTCGGCGGGTTTGTACTTGTGGAGTGCGAGATAGA‑TGAAGCACTTTCGGCGAGACTAGACCAAAATGAAAGCGCCTTATCg                                                >  1:392879/1‑151 (MQ=11)
                                                                                             gTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTACGGCGGGTTTGTAATTTTGGAGGGGCAGATATA‑AGAAACACAGTCCTCGACACTAGACACAAAAGCAACCGGATTATCgg                                               >  4:118865/1‑151 (MQ=11)
                                                                                               ccACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTGCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGTATCCCTGTCCGCGACACGATAGACAAAAGAAAGCGGCTGATCGGTc                                             >  1:1448915/1‑151 (MQ=11)
                                                                                                cACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTGCGGCGGGTTTTTGCTTTTGGAGGGGCAGAGAGA‑GGAATCCCTGTCGCCGACACTATACCAAAAAGAAAGCCGCTTATCGGta                                             >  3:1657194/1‑149 (MQ=11)
                                                                                                cACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑ATTCGGCGGGTTTTTAATTTTGGAGTGGCAGAAAGA‑TGAATCACTGTCCACGACACTATACACAAAAGAAAGCTGATTATCGGTc                                             >  2:1216777/1‑150 (MQ=11)
                                                                                                 aCGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAAAGACTGTCCACGACACTATCCCCAAAAGAAAGCGGCTTATCGGTCat                                           >  7:239404/1‑150 (MQ=25)
                                                                                                  cGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTAATTTTGGAGGGGCTGTAAGA‑TGAAAGACAGTCCACGACACGACACGCAAAAGAAAACGGCATACCGGACAGt                                          >  3:1727949/1‑151 (MQ=11)
                                                                                                      acTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑GTTCGGCGGGTTTTTACTTTTGGAGTGGGAGAAAGA‑TGAATGCCTGTCGACGTGACTATAGCCTAAAGAAAGCGGCTTATCGGTCAGtttt                                       >  3:1689518/1‑150 (MQ=11)
                                                                                                       cTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAccc                                                                                                                                     >  6:553103/1‑57 (MQ=35)
                                                                                                         ataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACAAC‑CTAAGAGCGTATTGTACTTTAAGAGTGTCACAAAAA‑TTATTGAAATACCACGACACAATAACAAAAAAAAAAAAGCTTATCGGTCAGTTTGACCt                                   >  6:1723370/1‑151 (MQ=2)
                                                                                                           aCCCAAACGAACGCGGCTTCTCGGTCAGTTTTTTTTTTTTTAATTATAATGCGT‑CTTCGGAGTTTTTTAAATTTTGGCTGTGTATTTCGA‑TTAACGACTGTCTTCGTATCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGa                                 <  6:967697/151‑1 (MQ=2)
                                                                                                           aCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCG‑ATTCGGCGGGTTTTAACTTTTGGACGGGCAGAAATA‑GCAATCACTGTCCACGACAATAACACAAAAAGAAACCGGCTTCTCgcacattgttaac                                    >  2:1553450/1‑136 (MQ=2)
                                                                                                              cAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGCTTTTTACTTTTGGAGGGCCAGAAAGA‑TCAATGCCTGTCCACGACACATTAACCAAAAGAAAGCGCCTTATCGCACAGTTTTACatgatg                               >  3:267753/1‑150 (MQ=14)
                                                                                                              cAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCG‑ATACGGCGGGTTTAAGATTTTTGAGTGCCATAAATA‑AAAAAGAAAGTAAAAGACACTAAAACCAAAAGAAAGAGGCTAATAGTTAAGATATACCTGAtat                              >  8:1611020/1‑149 (MQ=2)
                                                                                                               aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCGACGACACTATACCCAAAAGAAAGCGGCTTATCGGTAAGTATTAACTGATGt                              >  3:1278243/1‑150 (MQ=255)
                                                                                                                cacgctctcgctatctCGTTCATTTTCACCTGTTTTACGAAAAAACCCG‑CTTTTTTTTTTTTTTTATTTTGTAGTGGCAGAAATA‑TTAATTAATGTACACTACTCTATACCCAAAAGATATCGGGTTATCGGTCAGTTTTACCTGATGTAc                            <  4:1491158/136‑1 (MQ=255)
                                                                                                                 aagaaagCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACAG‑CTACGGCGGGTTTTTAATTTTGGAGGGCCATAAAGA‑ATAATCACAGTCAACGACACAATAAACAAAAGAAACCACCTTATCGCTCAGtaaaacatgatgatcc                           >  4:93968/1‑136 (MQ=2)
                                                                                                                  agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTTATGTACGt                          >  1:1001641/1‑151 (MQ=255)
                                                                                                                  agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCG‑CTTCGGCGGGTTTATACTTTTGGAGGGGCAGAAAGA‑AGAATGACTGTCCACGACACTATAAGCAAAAGAAAGCCGCTTAACGGTCAGTGTTAAATGATGTACGt                          >  5:355170/1‑151 (MQ=14)
                                                                                                                  acaacccGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTGCGGCGGTTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTa                                                   <  7:323639/120‑1 (MQ=255)
                                                                                                                   ggctcggcctaaggggTCTGTTTCAGCTGTTTTACTTAAAAACCCG‑CTTCGGCGGTTTTTTATTTTTGGAGGGGCAGAAAGA‑TCATTGACTGTCCTCGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGt                          <  5:1796621/136‑1 (MQ=14)
                                                                                                                   gaacccGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTTCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATAc                                                                     <  4:1260771/102‑1 (MQ=2)
                                                                                                                   gaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGt                          >  7:1579229/1‑150 (MQ=40)
                                                                                                                   gaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTCCGt                          >  7:197603/1‑150 (MQ=255)
                                                                                                                   gaaagCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGTGGCAGAAAGA‑TGAATGACTGTCCACGACACAATAAACAAAAGAAAGCGCCTTATCGGTCAGTTTTACCTGATGTACGt                          >  4:153911/1‑150 (MQ=14)
                                                                                                                    cgtgcggctacgagctCAGTTTCACCTGGTTTACGAAAAAACCCG‑CTTCGGCGGGTTTTTTCTTTTGGAGGGGCAGAAAGA‑TGAATGACTTTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaa                        <  5:1680808/136‑1 (MQ=11)
                                                                                                                     aagCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCG‑ATTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCGACGACACTATACACAAAAGAAAGCGGCTTATCGGTAAGTTTTAAATGATGTACGtaat                       >  4:1027582/1‑151 (MQ=14)
                                                                                                                       gCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGGAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTGATCGGTCAGTGTTAAATGAGGTACGttata                      >  8:1072773/1‑150 (MQ=11)
                                                                                                                       gCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAACCGGCTTATCGGTCAGTTTTACCTGATGTACGtaata                      >  7:666792/1‑150 (MQ=255)
                                                                                                                         ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTc                                                                                                                               >  3:1111744/1‑44 (MQ=35)
                                                                                                                         ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGAAATAAAc                   >  5:868704/1‑151 (MQ=255)
                                                                                                                          gCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACATGATGTACGTAATAAAcc                  >  8:1202790/1‑151 (MQ=255)
                                                                                                                          gCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACGATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAcc                  >  7:1329491/1‑151 (MQ=255)
                                                                                                                          gCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACAATATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTAACTGATCTACGTAATAAAcc                  >  4:1811901/1‑151 (MQ=255)
                                                                                                                          gCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑ATTCGGCGGGTTTTAGCTTTTTGAAGGGAAAAAAGA‑TGAATGAATGTCAACGACACTATACACAAAAGAAATCTTATTATAGGTCAGTTTGACCTGATGTACGTAATAAAcc                  >  6:1403104/1‑151 (MQ=11)
                                                                                                                           cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                 >  8:1052091/1‑151 (MQ=255)
                                                                                                                           cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                 >  3:225698/1‑151 (MQ=255)
                                                                                                                           cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGCCAGAAAGA‑AGAATGACAGTCCACGACACTATACCCAAAAGAAACCGCCTTAACGGTCAGTATTACATGATGTACGTAATAAACCg                 >  4:838926/1‑151 (MQ=14)
                                                                                                                            ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt                >  3:1792649/1‑151 (MQ=255)
                                                                                                                            ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt                >  5:1853297/1‑151 (MQ=255)
                                                                                                                             tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGCCACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt               >  6:721531/1‑151 (MQ=255)
                                                                                                                             tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCgat               >  6:833583/1‑149 (MQ=255)
                                                                                                                               tCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTATGATTTTTAAGGCGCAGAAAGA‑TCAATGACTGTAAAAGACTCTATAAAAAAAAAAAAGAGGCTTATCGGTCAGTTATAAATGATATACGTAATAAACCggtgc             >  8:675451/1‑147 (MQ=2)
                                                                                                                                gtgtgattttCACCTGTTTTA‑GTTAAAACCGGCCTGCCGCGGGTTTTTCTTTTTGCCGGCGC‑GAAATATTTATTGCCTTGACAAGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg            <  5:1294133/144‑1 (MQ=9)
                                                                                                                                cGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg            >  3:574913/1‑151 (MQ=255)
                                                                                                                                cGGTCAGTTTCACCTGATTTACGTAAAAAACAG‑CTTCGTCTAGTTTTTGCTTTTGGATCGTCAGAAAGA‑TGAATGACTGTCCACTAAACTATAAAAAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTAAGTAATGAAGCgcttcg            >  4:893836/1‑146 (MQ=2)
                                                                                                                                 ggTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTAACTGATGTACGTAATAAACCGTTCcgg           >  3:66942/1‑151 (MQ=255)
                                                                                                                                   tCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGCTGATCGtaagagcacacgtctgac         >  7:1063008/1‑136 (MQ=11)
                                                                                                                                   tCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTAAGCTTTTTGAGTGGCAGAAATA‑AGAAACACTGTCCACGACACAAAAAACAAAAGACAACAACTTATCGCACATTTAAAAATGATGTACATAA‑AAAccagtagcgaa         >  2:199536/1‑141 (MQ=0)
                                                                                                                                    cAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg        >  7:1756316/1‑151 (MQ=255)
                                                                                                                                    cAGTTTCACCTGATTTACGTAAAAACACG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACTCTATACCAAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATTTACGTAATaaaagaacggaagcg        >  2:1376695/1‑139 (MQ=11)
                                                                                                                                     aGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg       >  7:1389277/1‑151 (MQ=255)
                                                                                                                                     aGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg       >  3:1106613/1‑151 (MQ=255)
                                                                                                                                      gTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGt      >  1:328879/1‑151 (MQ=255)
                                                                                                                                         tCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc   >  8:350573/1‑151 (MQ=255)
                                                                                                                                          cACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCa  <  5:428519/151‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                        
AAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAGA‑TGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCA  >  DH1/613632‑613920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑