Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,1690T.31.1% 179.1 / 84.4 76intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (3/48);  new base . (1/22);  total (5/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01

GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATC  >  DH1/936019‑936289
                                                                                                                                                       |                                                                                                                         
gAATGGCTCTTTTATATTGGAGACCAGAAAAAAAAAAACACCCTTTATGTTGTTCAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                           <  4:1055550/151‑1 (MQ=18)
gAATGGCGCTTTTTTATGGGAGACCAGACACAAAAAACCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGGTATGAGCCCGACGAGCTACCAGGCTGCGCCACCCCGCGTCACCGTACTGCt                                                                                                                           <  3:624088/151‑1 (MQ=255)
gAAAGGCGCTTTTTTATGGGACACCAGAAACAAAAACACCCCTGTTAGGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                           <  1:464528/151‑1 (MQ=18)
 ataggggcTTTTATATGCGAGACCAGAAACAACAACACACCAGTTTTGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                           <  7:1531816/144‑1 (MQ=17)
 aatggttcgtttttATGGGAGACCTGTAACAATAAAAGACGCGTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                          <  4:1208692/142‑1 (MQ=17)
 aaTGGCGCTTTTTTATGTGATACAAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                          <  2:1093508/151‑1 (MQ=255)
 aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                          <  4:1112385/151‑1 (MQ=255)
 aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                          <  4:859236/151‑1 (MQ=255)
 aaTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                          <  3:932077/151‑1 (MQ=255)
 aaTGCCTCTTTTTTATGGGAGACCTGAAAAAAAAAATCACCCTTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                          <  8:1701639/151‑1 (MQ=18)
  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                         <  6:161602/151‑1 (MQ=255)
  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                         <  8:156827/151‑1 (MQ=255)
    ggCGCTTTTTTATTGGAGACCAGAAACAAAAAACAACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGTCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                       <  7:1578273/151‑1 (MQ=255)
    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                        <  6:697986/150‑1 (MQ=255)
    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                       <  8:1629618/151‑1 (MQ=255)
    ggCGCTTTTTTATGGGACCCCAGAACCAACAAAACCGCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttac                                                                                                                       <  1:1132646/151‑3 (MQ=18)
      ggcTTTTTTATGGGAGACCAGAAACAAATAAACACCGGTTAGGGTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttactt                                                                                                                     <  4:1097970/150‑5 (MQ=255)
      ggcTTTTTTATGGGAGACCAGAAACAAAAAAACACGCGTTAGGGTGTTCAATAATTGGTTGCGGGTGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                     <  6:1796957/150‑1 (MQ=255)
      cgcTTTTTTATGGGAGACCAGAAACAAAAAAACAGCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                     <  6:482141/151‑1 (MQ=255)
      cgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                     <  3:123196/151‑1 (MQ=255)
      cgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACt                                                                                                                     <  8:1638235/151‑1 (MQ=255)
       gcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACtt                                                                                                                    <  2:849019/151‑1 (MQ=255)
          tttttATGGAGACCAGATAAAAAAAATCACCTTTTTGGTTGATCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTtcat                                                                                                                 <  4:1690943/149‑1 (MQ=14)
           ttttATGGGAGACCAGAAACATAAAAACACCCGTTAGCGTGTTCAATAATTGGTAGCGGGGGCCGGATTTGAACCGATCACCTTCGGGTTATGAGCCCGACGAGCTACCAGCCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcat                                                                                                                 <  8:240241/150‑1 (MQ=21)
            tttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatc                                                                                                                <  7:1190754/150‑1 (MQ=255)
             ttATGGGAGACCAGATACAAAAATACAGCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAGTtcatca                                                                                                               <  8:382501/150‑1 (MQ=255)
             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                               <  1:399041/150‑1 (MQ=255)
             ttAGGGGACCCCAGAAAAAAAAAAACACCCTTTATGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCaa                                                                                                              <  1:1766289/150‑1 (MQ=14)
              tATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAAGCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                              <  6:953090/150‑1 (MQ=255)
               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaaa                                                                                                             <  3:1689496/150‑1 (MQ=255)
               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAt                                                                                                           <  6:30675/151‑1 (MQ=255)
                ggggACACCAGAAACCAAAAAAGACCCGTTAGGGTGTTCAATAATTGGGTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttt                                                                                                         <  7:1138254/150‑1 (MQ=17)
                 gggAGCCCAGAACCAAAAAAACCCCCTTTATGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttt                                                                                                         <  6:1593237/150‑1 (MQ=14)
                 gggAGACCTGAAACAAAAAAACACCCTTTATGGTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttt                                                                                                         <  2:1121064/150‑1 (MQ=17)
                 gggAGACCAGCAACACCAAAAGACGCTTGTGGGTGTTCATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAtttt                                                                                                        <  3:1417510/151‑2 (MQ=11)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAttt                                                                                                         <  7:1127612/151‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAttt                                                                                                         <  8:713516/151‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAtt                                                                                                          <  5:1824462/150‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAtt                                                                                                          <  4:156824/150‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAtt                                                                                                          <  4:1005810/150‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAtt                                                                                                          <  8:526952/150‑1 (MQ=255)
                 gggAGACCACATACAACAACACACCATTATTGTTGTTCTATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttt                                                                                                         <  3:152059/150‑1 (MQ=11)
                  ggAGACCTGATACAAATAATCAGTCGTTAGGGTGTTCATTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAtttta                                                                                                       <  2:964206/151‑3 (MQ=11)
                  ggAGACCAGATACAACAAAACACCCTTGAGGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAtttta                                                                                                       <  5:1559831/151‑3 (MQ=14)
                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTg                                                                                                        <  6:1648274/151‑1 (MQ=255)
                   gagaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGa                                                                                                       <  5:1827631/151‑1 (MQ=255)
                   gagaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGa                                                                                                       <  4:1334993/151‑1 (MQ=255)
                   gagaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGa                                                                                                       <  8:1260981/151‑1 (MQ=255)
                    agaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaat                                                                                                     <  4:1037075/151‑5 (MQ=255)
                    agaCCACAAACAAAAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaat                                                                                                     <  5:1823693/151‑5 (MQ=255)
                     gaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGAtt                                                                                                     <  5:1437622/151‑1 (MQ=255)
                     gaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGAtt                                                                                                     <  6:409811/151‑1 (MQ=255)
                     gaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGAtt                                                                                                     <  2:806257/151‑1 (MQ=255)
                      cccAGAAACACAAACACCCGCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaattg                                                                                                   <  5:1265170/150‑7 (MQ=14)
                      cccAGAAACAAAAAAACACCCGTGAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaattg                                                                                                   <  1:777758/150‑7 (MQ=255)
                      accactaccAAAAACACACCCTGTAGGGTGATCTATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaattg                                                                                                   <  3:1587398/143‑7 (MQ=11)
                      aCCAGAAACAAAAAAAGACCCTTTATGTTTTTAAATTATTGGTTGCGGGGGCTGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaattg                                                                                                   <  2:1182817/151‑7 (MQ=11)
                      aCCAGAAACAAAAAAAGACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGAtt                                                                                                     <  4:694495/150‑1 (MQ=255)
                      aCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTg                                                                                                    <  1:1475602/151‑1 (MQ=255)
                       cctcaaaCAATTAAAGCCCCTTTATGTTGTTAAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaattgg                                                                                                  <  2:752856/147‑8 (MQ=11)
                       ccAGAAACAACAAAAGAGCCGTTAGGGTGTAAATTAATTGGTTGCGGGGGCCGGATTTGACCCGAGGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaattgg                                                                                                  <  1:1543947/151‑8 (MQ=11)
                       ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGGCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTgg                                                                                                   <  2:1580860/151‑1 (MQ=255)
                       ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTgg                                                                                                   <  8:959248/151‑1 (MQ=255)
                       ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTgg                                                                                                   <  5:1560212/151‑1 (MQ=255)
                        cAGTAACAATTAAACAGCCTTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGAATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaattggt                                                                                                 <  8:1348269/151‑9 (MQ=11)
                         aGAAACAAAAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGtt                                                                                                 <  5:1733402/151‑1 (MQ=14)
                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGtt                                                                                                 <  5:186787/151‑1 (MQ=255)
                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGtt                                                                                                 <  3:837001/151‑1 (MQ=255)
                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGtt                                                                                                 <  7:859308/151‑1 (MQ=255)
                         aGAAAAAAAAACACACAATTTAGCTTGTTCAATCATTGGTTGCGGGTGCAGGATTTGAACCTACCACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGtt                                                                                                 <  2:76128/151‑1 (MQ=11)
                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTg                                                                                                <  6:331486/150‑1 (MQ=255)
                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGc                                                                                               <  1:335936/151‑1 (MQ=255)
                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGc                                                                                               <  2:115316/151‑1 (MQ=255)
                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTACGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGc                                                                                               <  1:1741154/151‑1 (MQ=255)
                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGCGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGc                                                                                               <  2:1646261/151‑1 (MQ=255)
                            aaCAAAAAACACACCAGTTAGGGTGTTCATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGATGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGc                                                                                               <  5:806735/151‑1 (MQ=11)
                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCg                                                                                              <  8:1029133/151‑1 (MQ=255)
                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATGGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCg                                                                                              <  6:733140/151‑1 (MQ=255)
                              cAAAAAAACACCCTTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGtt                                                                                                 <  8:1841478/146‑1 (MQ=11)
                              cAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATGAAATTTTAATTGGtttgatg                                                                                            >  7:1840477/1‑146 (MQ=14)
                               aaaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCgggg                                                                                           <  2:718829/151‑1 (MQ=255)
                               aaaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCgggg                                                                                           <  4:892325/151‑1 (MQ=255)
                                   aacacacCCGTTAGGGTGTTCAATAATTGGTTGCGTGGGCCGGATTTGCCCCGACGACCTTCGTGTTATCAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGcc                                                                                        <  6:32205/148‑1 (MQ=18)
                                          ccGGTAGGGTGTTCAATAATTTGTTCCGGGGGCCGGAGTTGCCCCGACGACCTTCGGGTTATGCGCCCGACGAGCGACCTTGCTTCGCCACCCCGCGTCACAGTACAGCGTCTACTTCATCAAAATTGATTGGTTGCGGGGGCCGGATTTg                                                                                 <  3:512128/151‑1 (MQ=17)
                                                  gtgtTCAATAATTGTTTGCGGGGGCCGGATTTTACCCGACGACCTTCGCGTTATCAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTTATTGGTTGCGGGGGCCGGATTTGAACcgacg                                                                         <  7:396720/151‑1 (MQ=14)
                                                      tCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                         <  7:748553/100‑1 (MQ=35)
                                                      tCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                         >  8:749148/1‑100 (MQ=35)
                                                               ggTTGCGTGGGGGGGAGTTGTTCGCACGTCCTTCGAGTTATCAGCCCGTCTAGCTTCCAGTCTTCTCCAGCCCTCTTCACATTACTGCTTTTACATCTTGATATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTa                                                             <  4:1220891/150‑1 (MQ=9)
                                                                                  gccccGACGACCGTCGGGTTATGAGCCCGAAGAGCGACCAGTCTTCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAg                                         <  7:1338390/148‑1 (MQ=14)
                                                                                                    ttATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGGAGTGCGTTTAGTCAAGCAAATTTTATTGGTTGAGGGGGGCGGATTTGAACCGCCGACCTTAGGGTTATGAGCCAGACGAGCTAGCAGGCTGCTCCACCCCGCGt                        >  7:1036134/1‑150 (MQ=11)
                                                                                                                          ggCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGTAATGCTTGTATtcatc  >  5:1765227/1‑150 (MQ=14)
                                                                                                                                         cgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGa                                                                                     >  6:1020149/1‑52 (MQ=255)
                                                                                                                                              ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTccg                     >  5:1536298/1‑109 (MQ=255)
                                                                                                                                                       |                                                                                                                         
GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATC  >  DH1/936019‑936289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: