Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2781.T26.7% 206.4 / 118.3 86intergenic (‑12/+22)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base . (59/4);  new base T (22/1);  total (81/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-03

GGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTT  >  DH1/936140‑936427
                                                                                                                                            |                                                                                                                                                     
ggCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGTAATGCTTGTATtcatc                                                                                                                                            >  5:1765227/1‑150 (MQ=14)
                                        caAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCACATTATAATTGCTTGCGGCGGCCGGATTTGAACcgacgcc                                                                                                   >  7:1041003/1‑149 (MQ=255)
                                              tGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACGGTACTGCGTTACTTCTGCAAAGTTTAATTGGTTGCGGGGGACGGATTTGCAGCGACGACCTTAgg                                                                                             >  4:300102/1‑151 (MQ=14)
                                                                  aTTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAt                                                                                                                                        <  7:1544602/88‑1 (MQ=35)
                                                                  aTTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAt                                                                                                                                        >  8:1545991/1‑88 (MQ=35)
                                                                       aaCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGACCGCCGAGCTACAAgg                                                                    >  5:834976/1‑151 (MQ=255)
                                                                                 cTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAATAATAAAATTTTAATTGGTTGCGGGGGCCGGAtt                                                                                                               >  6:865261/1‑98 (MQ=14)
                                                                                  ttCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACGGGACGGGGTGACTTCATCACAGGTTTATTGGTTGCCGGTGGATGTTTTGCAGCTCCGCCCTTCGGGTTGTTAGACCGAAGAGCGACAAGGCTGCTCcccc                                                          >  1:642040/1‑150 (MQ=2)
                                                                                        ttATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACGTCATCAAATTTTAATTGGTTg                                                                                                                            >  6:198575/1‑78 (MQ=255)
                                                                                               cccGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGGACTGCTTTAATTCAGCAAATTTTAATTCCTTGCCGGTGACGGTTAAGAACCTACCCCATTAGGCATATGATACAGCCGACCAACCAGGCTGCACCAACAAGCGTcagacaa                                             >  1:1551729/1‑144 (MQ=9)
                                                                                                         tACCAGGCTGCTCCACCCCGCGTCACGGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGGCGGATTTGCACCGACGACCTTCGGGTTATGAGCGCGACGAGCTACCAGGCTGCTCCACCAGGCGTCCGTGGATGAGCACtat                                   >  7:271342/1‑150 (MQ=17)
                                                                                                            cAGGCTGCTCCACCCCGCGTCACCGTAGTCGGATTATTCAGCAAATTTTAATTGGTTGCGGGGGGATTATTGGACGAGACGACCTTAGGCTTAAGAGACCGAAGAGCTACCAGGCTGCTCCCCCCCGATTCCGTGGAAGATGACTATACt                                >  6:45256/1‑150 (MQ=9)
                                                                                                             aGGCTGCTCCACCCCGCGTCACCGTACTGCTTGACTTCATCAAATTTTAATTGGTTGCGGGGGGCGGATTTGACCCGACGCCCTTCGGGTTATGAGCCCGACTAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTATGCGCACTAtcctcc                              >  3:1351143/1‑151 (MQ=17)
                                                                                                               gctgctCCACCCCGCGTCACCGTACTCCGTTAATTCATCAAAATTTAATTCGTTGCGGCGGAAGTATAAGACCCGCCGACCTTCGGGTTATGAGACCGAACAACTAACACACTGCTCCAAACATCGTCCGTTGATTCGCACTATACTCCGc                            >  4:953072/1‑151 (MQ=12)
                                                                                                                 tgctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCTCGTCCGTGGATGCGCACTATACTCCGGTc                          >  2:560162/1‑151 (MQ=255)
                                                                                                                 tgctCCACCCCGCGTCACCGTACTGCTTTAATTCATCAAATTTTAATTGGTTCCCCCCGCCTGATTAGAAACGACGACCTTCCGGATATCAGACCGACGAGCTACCAGGCTACTCCACCCCTAGTCCGTGGATTAGCACTATACTcacctc                          >  2:1050526/1‑146 (MQ=12)
                                                                                                                  gctCCACCCCGCGTCCCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCCCCCCGCTTCCGTGGATGCGCACTATACTCGGCTcg                         >  6:1699138/1‑151 (MQ=255)
                                                                                                                  gctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCACCCGCGTCCGTGGATGCGCACTATACTCCGCTcg                         >  6:661367/1‑151 (MQ=255)
                                                                                                                   ctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgc                        >  6:1764551/1‑151 (MQ=255)
                                                                                                                    tCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                       >  5:1865108/1‑151 (MQ=255)
                                                                                                                      cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCGGGATTTGAACCGACGACCTTCGGGTGATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCATCCCTGCATGCGCACTATACTCCGCTCGCGtt                     >  3:1619069/1‑151 (MQ=255)
                                                                                                                      cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                     >  8:318888/1‑151 (MQ=255)
                                                                                                                      cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                     >  5:289127/1‑151 (MQ=255)
                                                                                                                       aCCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                    >  3:952395/1‑151 (MQ=255)
                                                                                                                        ccccGCGTCACCGTACTCGTTTTACTTAATAAAATTTTATTGGTTGCGGGGGCCTGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACCAGGTACCCGGCTGCTCCACCCCGCGACCCAGGATGCGGACTATACtgcaaactagtatt                   >  8:897397/1‑138 (MQ=11)
                                                                                                                         cccGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATTCGCACTATACTCCGATAGAGTTTTc                  >  1:1447990/1‑151 (MQ=11)
                                                                                                                         cccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                  >  1:1207206/1‑151 (MQ=255)
                                                                                                                          ccGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACTATACTCCGCTGGGGTTTTc                  >  3:816714/1‑150 (MQ=11)
                                                                                                                          ccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                  >  3:1487115/1‑150 (MQ=255)
                                                                                                                           cgcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCTCTATACTCCGCTCGAGttttggt                >  3:1230728/1‑148 (MQ=14)
                                                                                                                           cgcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGCAGTATACTGCgaaggagtattcat                >  8:1598142/1‑138 (MQ=11)
                                                                                                                           cgcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCCGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATGCGGACTATACTACCCTCTCGTTTTCAt                >  4:684565/1‑151 (MQ=11)
                                                                                                                           cgcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                >  5:1449442/1‑151 (MQ=255)
                                                                                                                            gcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATGCGCAGAATACTCCGATCGCGTTTTGAtt               >  5:469719/1‑150 (MQ=11)
                                                                                                                            gcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg               >  6:1109793/1‑151 (MQ=255)
                                                                                                                            gcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg               >  1:970812/1‑151 (MQ=255)
                                                                                                                            gcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTATTCATg               >  8:700622/1‑151 (MQ=255)
                                                                                                                            gcgTCACCGTACTGCGTTACTTCTTGAAAGTTTAATTGGTTGCGGGGGCCGGATTTGCACAGACGCCCGTCGGGGTATGAGCCCGACGAGCTACCAGGCTGCTGCAGCCCGAGTCCGTGGATGGGCACTATACTCCGCTCGCGTTTTCATg               >  2:383409/1‑151 (MQ=18)
                                                                                                                             cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGa                                                                                                                 <  5:1020491/52‑1 (MQ=255)
                                                                                                                             cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc              >  2:594592/1‑151 (MQ=255)
                                                                                                                             cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc              >  8:731934/1‑151 (MQ=255)
                                                                                                                               tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATGCCCAGTATACACCGCAGTCGTTTTccttcc             >  4:163669/1‑145 (MQ=11)
                                                                                                                               tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAATATACTCCGCtatatatttgcttca             >  4:942944/1‑136 (MQ=11)
                                                                                                                               tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAGGCCCACTATACACCCCAAGAGTTTTCAttcc             >  4:951148/1‑147 (MQ=11)
                                                                                                                               tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa             >  5:730997/1‑150 (MQ=255)
                                                                                                                               tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa             >  6:1375145/1‑150 (MQ=255)
                                                                                                                               tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa             >  8:512550/1‑150 (MQ=255)
                                                                                                                                cACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc           >  4:469389/1‑151 (MQ=255)
                                                                                                                                cACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc           >  1:655624/1‑151 (MQ=255)
                                                                                                                                aaCCTTACTGCTTTAACTCATCAAAATTTAATTTGTTGCGGTGTCCGGATTTTATACGATGACCTTCTGTTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc           <  8:1037044/150‑1 (MQ=21)
                                                                                                                                 aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATTCGGAGTATACTGCGCTGGGGTTTTCCTggctcg          >  6:1167728/1‑146 (MQ=11)
                                                                                                                                 aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAGTCGCACTATACTACGATCGCGTTTTCATTCAccc          >  5:316079/1‑151 (MQ=11)
                                                                                                                                 aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAGGCGCTCTATACTCCGCAGTCTTTTTCAttgaacg          >  5:129346/1‑145 (MQ=11)
                                                                                                                                 aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc          >  5:909624/1‑151 (MQ=255)
                                                                                                                                 aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc          >  6:414041/1‑151 (MQ=255)
                                                                                                                                 aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc          >  2:11982/1‑151 (MQ=255)
                                                                                                                                  ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                <  4:333268/144‑1 (MQ=255)
                                                                                                                                  ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTACGCTAGCGTTTTCAt                >  3:332263/1‑144 (MQ=14)
                                                                                                                                  ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCGGAAAATACTGCACAAGCGTATTCATGCAGccg         >  5:141426/1‑150 (MQ=11)
                                                                                                                                  ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATGCCGACTATACTACGATCTCGATTTCATGCACCCt         >  1:137919/1‑151 (MQ=11)
                                                                                                                                   cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt        >  1:1489876/1‑151 (MQ=255)
                                                                                                                                   cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt        >  1:406535/1‑151 (MQ=255)
                                                                                                                                   cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt        >  2:782252/1‑151 (MQ=255)
                                                                                                                                   cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt        >  3:839569/1‑151 (MQ=255)
                                                                                                                                    gTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTAGCGTTTTCATGCAACCttt       >  3:1381597/1‑151 (MQ=17)
                                                                                                                                    gTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAATATACTACGCTCGAGTTTTCATTCAACCttt       >  6:1198890/1‑151 (MQ=14)
                                                                                                                                    gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt       >  6:1259201/1‑151 (MQ=255)
                                                                                                                                    gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt       >  1:1601731/1‑151 (MQ=255)
                                                                                                                                    gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt       >  4:1228860/1‑151 (MQ=255)
                                                                                                                                    gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt       >  4:561409/1‑151 (MQ=255)
                                                                                                                                     tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATTCGCAATATACTACAATAGAGTATTCATTCAAccgtgt      >  6:729593/1‑147 (MQ=11)
                                                                                                                                     tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAGGGGCACTATACACCCCTCTCGATTTCCTGGAACCtgct      >  3:1433776/1‑148 (MQ=11)
                                                                                                                                     tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt      >  8:736084/1‑151 (MQ=255)
                                                                                                                                     tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt      >  7:892207/1‑151 (MQ=255)
                                                                                                                                      aCTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAGTATACTGCGCAGGGGTTTTGATGCAAcggtgtt     >  5:434130/1‑145 (MQ=11)
                                                                                                                                      aCTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCTTTTTCATGCAACCttttt     >  7:260373/1‑151 (MQ=17)
                                                                                                                                      aCTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAATCGCACTATACTCCGCTCTAGTTTTCATGCACCCttttt     >  1:941231/1‑151 (MQ=14)
                                                                                                                                      aCTGCTTTATTTCATTTTTTTTTTATTTGTTTCGGGTGCTGTATTTCAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt      <  7:390713/150‑1 (MQ=21)
                                                                                                                                      aCTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt     >  7:1007753/1‑151 (MQ=255)
                                                                                                                                      aCTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt     >  4:668238/1‑151 (MQ=255)
                                                                                                                                      aCTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt     >  1:287260/1‑151 (MQ=255)
                                                                                                                                       cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt    >  7:59094/1‑151 (MQ=255)
                                                                                                                                        tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt   >  3:1275505/1‑151 (MQ=255)
                                                                                                                                        tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt   >  8:566579/1‑151 (MQ=255)
                                                                                                                                          gCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  5:1597173/1‑151 (MQ=255)
                                                                                                                                          gCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  6:1053907/1‑151 (MQ=255)
                                                                                                                                           cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  7:1167601/1‑150 (MQ=255)
                                                                                                                                           cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  4:487999/1‑150 (MQ=255)
                                                                                                                                           cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACAAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt  >  5:1048897/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                                     
GGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTT  >  DH1/936140‑936427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: