Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2930T.31.7% 338.7 / 177.0 151intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (91/8);  new base . (44/2);  total (139/12)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-03

Only 100 of 153 total aligned reads displayed.

CAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAT  >  DH1/936180‑936442
                                                                                                                   |                                                                                                                                                     
caAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCACATTATAATTGCTTGCGGCGGCCGGATTTGAACcgacgcc                                                                                                                  >  7:1041003/1‑149 (MQ=255)
      tGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACGGTACTGCGTTACTTCTGCAAAGTTTAATTGGTTGCGGGGGACGGATTTGCAGCGACGACCTTAgg                                                                                                            >  4:300102/1‑151 (MQ=14)
                          aTTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAt                                                                                                                                                       <  7:1544602/88‑1 (MQ=35)
                                         cTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAATAATAAAATTTTAATTGGTTGCGGGGGCCGGAtt                                                                                                                              >  6:865261/1‑98 (MQ=14)
                                                ttATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACGTCATCAAATTTTAATTGGTTg                                                                                                                                           >  6:198575/1‑78 (MQ=255)
                                                                    cAGGCTGCTCCACCCCGCGTCACCGTAGTCGGATTATTCAGCAAATTTTAATTGGTTGCGGGGGGATTATTGGACGAGACGACCTTAGGCTTAAGAGACCGAAGAGCTACCAGGCTGCTCCCCCCCGATTCCGTGGAAGATGACTATACt                                               >  6:45256/1‑150 (MQ=9)
                                                                     aGGCTGCTCCACCCCGCGTCACCGTACTGCTTGACTTCATCAAATTTTAATTGGTTGCGGGGGGCGGATTTGACCCGACGCCCTTCGGGTTATGAGCCCGACTAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTATGCGCACTAtcctcc                                             >  3:1351143/1‑151 (MQ=17)
                                                                         tgctCCACCCCGCGTCACCGTACTGCTTTAATTCATCAAATTTTAATTGGTTCCCCCCGCCTGATTAGAAACGACGACCTTCCGGATATCAGACCGACGAGCTACCAGGCTACTCCACCCCTAGTCCGTGGATTAGCACTATACTcacctc                                         >  2:1050526/1‑146 (MQ=12)
                                                                          gctCCACCCCGCGTCCCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCCCCCCGCTTCCGTGGATGCGCACTATACTCGGCTcg                                        >  6:1699138/1‑151 (MQ=255)
                                                                           ctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgc                                       >  6:1764551/1‑151 (MQ=255)
                                                                              cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCGGGATTTGAACCGACGACCTTCGGGTGATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCATCCCTGCATGCGCACTATACTCCGCTCGCGtt                                    >  3:1619069/1‑151 (MQ=255)
                                                                              cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                    >  8:318888/1‑151 (MQ=255)
                                                                              cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                    >  5:289127/1‑151 (MQ=255)
                                                                                 cccGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATTCGCACTATACTCCGATAGAGTTTTc                                 >  1:1447990/1‑151 (MQ=11)
                                                                                 cccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                 >  1:1207206/1‑151 (MQ=255)
                                                                                   cgcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCTCTATACTCCGCTCGAGttttggt                               >  3:1230728/1‑148 (MQ=14)
                                                                                   cgcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGCAGTATACTGCgaaggagtattcat                               >  8:1598142/1‑138 (MQ=11)
                                                                                   cgcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCCGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATGCGGACTATACTACCCTCTCGTTTTCAt                               >  4:684565/1‑151 (MQ=11)
                                                                                   cgcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                               >  5:1449442/1‑151 (MQ=255)
                                                                                    gcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                              >  1:970812/1‑151 (MQ=255)
                                                                                    gcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTATTCATg                              >  8:700622/1‑151 (MQ=255)
                                                                                    gcgTCACCGTACTGCGTTACTTCTTGAAAGTTTAATTGGTTGCGGGGGCCGGATTTGCACAGACGCCCGTCGGGGTATGAGCCCGACGAGCTACCAGGCTGCTGCAGCCCGAGTCCGTGGATGGGCACTATACTCCGCTCGCGTTTTCATg                              >  2:383409/1‑151 (MQ=18)
                                                                                     cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGa                                                                                                                                <  5:1020491/52‑1 (MQ=255)
                                                                                     cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                             >  2:594592/1‑151 (MQ=255)
                                                                                       tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATGCCCAGTATACACCGCAGTCGTTTTccttcc                            >  4:163669/1‑145 (MQ=11)
                                                                                       tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAGGCCCACTATACACCCCAAGAGTTTTCAttcc                            >  4:951148/1‑147 (MQ=11)
                                                                                       tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                            >  8:512550/1‑150 (MQ=255)
                                                                                       tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                            >  5:730997/1‑150 (MQ=255)
                                                                                        cACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                          >  1:655624/1‑151 (MQ=255)
                                                                                        aaCCTTACTGCTTTAACTCATCAAAATTTAATTTGTTGCGGTGTCCGGATTTTATACGATGACCTTCTGTTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                          <  8:1037044/150‑1 (MQ=21)
                                                                                         aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATTCGGAGTATACTGCGCTGGGGTTTTCCTggctcg                         >  6:1167728/1‑146 (MQ=11)
                                                                                         aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAGTCGCACTATACTACGATCGCGTTTTCATTCAccc                         >  5:316079/1‑151 (MQ=11)
                                                                                         aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAGGCGCTCTATACTCCGCAGTCTTTTTCAttgaacg                         >  5:129346/1‑145 (MQ=11)
                                                                                         aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                         >  2:11982/1‑151 (MQ=255)
                                                                                         aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                         >  6:414041/1‑151 (MQ=255)
                                                                                         aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                         >  5:909624/1‑151 (MQ=255)
                                                                                          ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                               <  4:333268/144‑1 (MQ=255)
                                                                                          ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTACGCTAGCGTTTTCAt                               >  3:332263/1‑144 (MQ=14)
                                                                                           cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                       >  1:1489876/1‑151 (MQ=255)
                                                                                           cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                       >  3:839569/1‑151 (MQ=255)
                                                                                           cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                       >  2:782252/1‑151 (MQ=255)
                                                                                            gTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTAGCGTTTTCATGCAACCttt                      >  3:1381597/1‑151 (MQ=17)
                                                                                            gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                      >  6:1259201/1‑151 (MQ=255)
                                                                                            gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                      >  4:1228860/1‑151 (MQ=255)
                                                                                            gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                      >  1:1601731/1‑151 (MQ=255)
                                                                                             tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATTCGCAATATACTACAATAGAGTATTCATTCAAccgtgt                     >  6:729593/1‑147 (MQ=11)
                                                                                             tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAGGGGCACTATACACCCCTCTCGATTTCCTGGAACCtgct                     >  3:1433776/1‑148 (MQ=11)
                                                                                             tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                     >  7:892207/1‑151 (MQ=255)
                                                                                             tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                     >  8:736084/1‑151 (MQ=255)
                                                                                              aCTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAGTATACTGCGCAGGGGTTTTGATGCAAcggtgtt                    >  5:434130/1‑145 (MQ=11)
                                                                                              aCTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCTTTTTCATGCAACCttttt                    >  7:260373/1‑151 (MQ=17)
                                                                                              aCTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAATCGCACTATACTCCGCTCTAGTTTTCATGCACCCttttt                    >  1:941231/1‑151 (MQ=14)
                                                                                              aCTGCTTTATTTCATTTTTTTTTTATTTGTTTCGGGTGCTGTATTTCAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                     <  7:390713/150‑1 (MQ=21)
                                                                                               cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                   >  7:59094/1‑151 (MQ=255)
                                                                                                tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                  >  8:566579/1‑151 (MQ=255)
                                                                                                  gcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGTAAGCCAAATATACTACGCTCTCGTATTCATGCAGCCttttttat                 >  2:538587/4‑149 (MQ=11)
                                                                                                  gCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                 >  5:1597173/1‑151 (MQ=255)
                                                                                                  gCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                 >  6:1053907/1‑151 (MQ=255)
                                                                                                  agctttcctgaagcaAATTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                   <  1:378504/136‑1 (MQ=18)
                                                                                                   tgttaagttatatcaaTTTTTATTGGTTGCGGGGGCTGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                  <  8:930655/136‑1 (MQ=21)
                                                                                                   cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                 >  7:1167601/1‑150 (MQ=255)
                                                                                                   cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                 >  4:487999/1‑150 (MQ=255)
                                                                                                   cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACAAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                 >  5:1048897/1‑150 (MQ=255)
                                                                                                    ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCAATATACTCCGATAGATTTTTCATTCAACCTTTTTTTTa                >  8:1143227/2‑150 (MQ=14)
                                                                                                    ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAGGCCGACTATACTACCCAGGATTTTTCATGCACCCGTGGTTTTAc               >  6:1135610/2‑151 (MQ=1)
                                                                                                    ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAATCGCAATATACTACGAACGCGTTTTCATGCAACCTTAGTTTTAc               >  2:1828402/2‑151 (MQ=14)
                                                                                                    gttttactgcagaaaTTTTCATTGGTTGCGGGTGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTa                <  5:411903/139‑1 (MQ=21)
                                                                                                     tttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGACTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGTATGCGCAAAATACTACGCTCGTTATTTCATGCACCCTTTATTTTAcc              >  1:1000881/1‑151 (MQ=14)
                                                                                                      ttacctccaccaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTa                <  5:1168108/138‑1 (MQ=255)
                                                                                                      ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc              >  6:787764/1‑151 (MQ=255)
                                                                                                       tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc             >  4:671853/1‑151 (MQ=255)
                                                                                                       tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc             >  3:392416/1‑151 (MQ=255)
                                                                                                       tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc             >  4:648225/1‑151 (MQ=255)
                                                                                                       tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATTCACCCTTTTTTTTAaccc            >  3:1476493/1‑151 (MQ=18)
                                                                                                        aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATTAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGCGTATGCCGACTATACTCCCCTCGCGTTTTCATGGACCGTTTTTTTTAgcccc           >  7:1695270/1‑150 (MQ=14)
                                                                                                        aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGCTCTATACACCCCTATAGATTTCCTTCACCCTTATTTTTAacccc           >  1:327412/1‑150 (MQ=11)
                                                                                                        aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGGAATATACTCCGATAGCGTATTCATTCAGCCTTTTTTTTACCCCg           >  4:1409363/1‑151 (MQ=14)
                                                                                                         cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTGTTCATGCAACCTTTTTTTTACCCCg           >  7:9054/1‑151 (MQ=255)
                                                                                                         cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCACACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg           >  3:835003/1‑151 (MQ=255)
                                                                                                          ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg           >  6:1702565/1‑150 (MQ=255)
                                                                                                          ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGCGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg           >  2:955384/1‑150 (MQ=255)
                                                                                                          ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg           >  3:1508646/1‑150 (MQ=255)
                                                                                                          ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg           >  2:1286041/1‑150 (MQ=255)
                                                                                                          ttCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGTAGTCGCAATATACTGCGCTGTAGTTTTCCTGCACCCTGTGTTTTTCCCCgcg         >  1:851338/1‑149 (MQ=11)
                                                                                                           tcatcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGCGTTATGAGCCCGACGAGCTACAAGGCTCCTCCACCCCGCGTCCGAGGATGCGAAAAATTCTACACTCGCCTTTTCCTTCAAACTTAATTTTAaccacaa         >  8:836452/1‑146 (MQ=2)
                                                                                                            tatgaaaCTTTCATTGGGTGCTTGGTCCGGAATTGTACCTACGACCTTCGCGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt         <  4:610430/146‑1 (MQ=25)
                                                                                                            catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc        >  1:502755/1‑151 (MQ=255)
                                                                                                            catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc        >  8:691858/1‑151 (MQ=255)
                                                                                                            catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc        >  1:1708266/1‑151 (MQ=255)
                                                                                                             atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc        >  2:1790238/1‑150 (MQ=255)
                                                                                                             atcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTGCTCTGGATTTTTCATGCACCGGTGTTTTTAGCACGATCAc      >  7:1002884/1‑151 (MQ=14)
                                                                                                              tcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc      >  1:630575/1‑151 (MQ=255)
                                                                                                              tcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc      >  7:1288061/1‑151 (MQ=255)
                                                                                                              tcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc      >  4:258998/1‑151 (MQ=255)
                                                                                                               caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg     >  4:484366/1‑151 (MQ=255)
                                                                                                                aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg    >  7:158374/1‑151 (MQ=255)
                                                                                                                 aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg    >  4:750350/1‑150 (MQ=255)
                                                                                                                 aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGa   <  3:673197/151‑1 (MQ=255)
                                                                                                                  aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt  >  5:831408/1‑151 (MQ=255)
                                                                                                                  aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTATTTTTTACCCCGATCACGGAt  >  4:835776/1‑151 (MQ=255)
                                                                                                                   |                                                                                                                                                     
CAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAT  >  DH1/936180‑936442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: