Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2970AG26.1% 415.3 / 152.3 207intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (122/25);  new base G (50/2);  total (174/33)
Fisher's exact test for biased strand distribution p-value = 1.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.03e-03

Only 100 of 208 total aligned reads displayed.

CAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936180‑936447
                                                                                                                       |                                                                                                                                                      
caAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCACATTATAATTGCTTGCGGCGGCCGGATTTGAACcgacgcc                                                                                                                       >  7:1041003/1‑149 (MQ=255)
                                         cTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAATAATAAAATTTTAATTGGTTGCGGGGGCCGGAtt                                                                                                                                   >  6:865261/1‑98 (MQ=14)
                                          ttCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACGGGACGGGGTGACTTCATCACAGGTTTATTGGTTGCCGGTGGATGTTTTGCAGCTCCGCCCTTCGGGTTGTTAGACCGAAGAGCGACAAGGCTGCTCcccc                                                                              >  1:642040/1‑150 (MQ=2)
                                                ttATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACGTCATCAAATTTTAATTGGTTg                                                                                                                                                >  6:198575/1‑78 (MQ=255)
                                                                 tACCAGGCTGCTCCACCCCGCGTCACGGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGGCGGATTTGCACCGACGACCTTCGGGTTATGAGCGCGACGAGCTACCAGGCTGCTCCACCAGGCGTCCGTGGATGAGCACtat                                                       >  7:271342/1‑150 (MQ=17)
                                                                     aGGCTGCTCCACCCCGCGTCACCGTACTGCTTGACTTCATCAAATTTTAATTGGTTGCGGGGGGCGGATTTGACCCGACGCCCTTCGGGTTATGAGCCCGACTAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTATGCGCACTAtcctcc                                                  >  3:1351143/1‑151 (MQ=17)
                                                                          gctCCACCCCGCGTCCCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCCCCCCGCTTCCGTGGATGCGCACTATACTCGGCTcg                                             >  6:1699138/1‑151 (MQ=255)
                                                                            tCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                                           >  5:1865108/1‑151 (MQ=255)
                                                                                ccccGCGTCACCGTACTCGTTTTACTTAATAAAATTTTATTGGTTGCGGGGGCCTGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACCAGGTACCCGGCTGCTCCACCCCGCGACCCAGGATGCGGACTATACtgcaaactagtatt                                       >  8:897397/1‑138 (MQ=11)
                                                                                  ccGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACTATACTCCGCTGGGGTTTTc                                      >  3:816714/1‑150 (MQ=11)
                                                                                   cgcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCTCTATACTCCGCTCGAGttttggt                                    >  3:1230728/1‑148 (MQ=14)
                                                                                   cgcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGCAGTATACTGCgaaggagtattcat                                    >  8:1598142/1‑138 (MQ=11)
                                                                                   cgcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCCGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATGCGGACTATACTACCCTCTCGTTTTCAt                                    >  4:684565/1‑151 (MQ=11)
                                                                                    gcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                                   >  1:970812/1‑151 (MQ=255)
                                                                                     cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                                  >  2:594592/1‑151 (MQ=255)
                                                                                     cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                                  >  8:731934/1‑151 (MQ=255)
                                                                                       tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAGGCCCACTATACACCCCAAGAGTTTTCAttcc                                 >  4:951148/1‑147 (MQ=11)
                                                                                        cACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                               >  4:469389/1‑151 (MQ=255)
                                                                                        aaCCTTACTGCTTTAACTCATCAAAATTTAATTTGTTGCGGTGTCCGGATTTTATACGATGACCTTCTGTTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                               <  8:1037044/150‑1 (MQ=21)
                                                                                         aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAGTCGCACTATACTACGATCGCGTTTTCATTCAccc                              >  5:316079/1‑151 (MQ=11)
                                                                                         aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                              >  5:909624/1‑151 (MQ=255)
                                                                                         aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                              >  6:414041/1‑151 (MQ=255)
                                                                                          ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                                    <  4:333268/144‑1 (MQ=255)
                                                                                          ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTACGCTAGCGTTTTCAt                                    >  3:332263/1‑144 (MQ=14)
                                                                                          ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATGCCGACTATACTACGATCTCGATTTCATGCACCCt                             >  1:137919/1‑151 (MQ=11)
                                                                                           cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                            >  1:1489876/1‑151 (MQ=255)
                                                                                            gTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAATATACTACGCTCGAGTTTTCATTCAACCttt                           >  6:1198890/1‑151 (MQ=14)
                                                                                            gTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                           >  1:1601731/1‑151 (MQ=255)
                                                                                             tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATTCGCAATATACTACAATAGAGTATTCATTCAAccgtgt                          >  6:729593/1‑147 (MQ=11)
                                                                                             tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                          >  7:892207/1‑151 (MQ=255)
                                                                                              aCTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCTTTTTCATGCAACCttttt                         >  7:260373/1‑151 (MQ=17)
                                                                                              aCTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                         >  1:287260/1‑151 (MQ=255)
                                                                                              aCTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                         >  4:668238/1‑151 (MQ=255)
                                                                                              aCTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                         >  7:1007753/1‑151 (MQ=255)
                                                                                               cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                        >  7:59094/1‑151 (MQ=255)
                                                                                                tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                       >  8:566579/1‑151 (MQ=255)
                                                                                                  gcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGTAAGCCAAATATACTACGCTCTCGTATTCATGCAGCCttttttat                      >  2:538587/4‑149 (MQ=11)
                                                                                                  gCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                      >  6:1053907/1‑151 (MQ=255)
                                                                                                  agctttcctgaagcaAATTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                        <  1:378504/136‑1 (MQ=18)
                                                                                                   cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                      >  7:1167601/1‑150 (MQ=255)
                                                                                                   cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                      >  4:487999/1‑150 (MQ=255)
                                                                                                   cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACAAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                      >  5:1048897/1‑150 (MQ=255)
                                                                                                    ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCAATATACTCCGATAGATTTTTCATTCAACCTTTTTTTTa                     >  8:1143227/2‑150 (MQ=14)
                                                                                                    ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAGGCCGACTATACTACCCAGGATTTTTCATGCACCCGTGGTTTTAc                    >  6:1135610/2‑151 (MQ=1)
                                                                                                    ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAATCGCAATATACTACGAACGCGTTTTCATGCAACCTTAGTTTTAc                    >  2:1828402/2‑151 (MQ=14)
                                                                                                    gttttactgcagaaaTTTTCATTGGTTGCGGGTGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTa                     <  5:411903/139‑1 (MQ=21)
                                                                                                     tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTGTTTTTTAc                    >  8:330548/1‑151 (MQ=255)
                                                                                                     tttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGACTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGTATGCGCAAAATACTACGCTCGTTATTTCATGCACCCTTTATTTTAcc                   >  1:1000881/1‑151 (MQ=14)
                                                                                                      ttacctccaccaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTa                     <  5:1168108/138‑1 (MQ=255)
                                                                                                      ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc                   >  6:787764/1‑151 (MQ=255)
                                                                                                      ttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAGGCGCACTATACTACGATCGAGTTTTCATGCAACCGTTTTtatatcg                  >  2:379980/1‑148 (MQ=14)
                                                                                                       tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc                  >  3:392416/1‑151 (MQ=255)
                                                                                                       tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc                  >  3:1394643/1‑151 (MQ=255)
                                                                                                       tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATTCACCCTTTTTTTTAaccc                 >  3:1476493/1‑151 (MQ=18)
                                                                                                       tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAATCCCACAATACTACCCTCGAGATTTCATTCAACCTTTATTATAaccc                 >  6:1737023/1‑151 (MQ=11)
                                                                                                        tactcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcccc                 >  3:633752/4‑151 (MQ=255)
                                                                                                        aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGAGTTTTCATGCAACCTTATTTTTAacccc                >  2:1226828/1‑150 (MQ=18)
                                                                                                        aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGGAATATACTCCGATAGCGTATTCATTCAGCCTTTTTTTTACCCCg                >  4:1409363/1‑151 (MQ=14)
                                                                                                        aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACAATACTCCGATGGATTTTTCATTCACCCTTTTTTTTAgcccc                >  1:992094/1‑150 (MQ=14)
                                                                                                        aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCCGGAGGCGCACAATACTACGCTCGCTATTTCATGCACCCGTTTGTTTagcacg                >  7:1199203/1‑146 (MQ=11)
                                                                                                         ttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGATAGAGTTTTCATGCAAcc                              >  8:741907/14‑137 (MQ=14)
                                                                                                         ttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCCCACTATACTCCGCTCGCGTTTTCCTGCCAGCTTGTTTTTAccccc                >  4:9043/14‑150 (MQ=14)
                                                                                                         cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                >  1:8900/1‑151 (MQ=255)
                                                                                                          ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                >  3:1508646/1‑150 (MQ=255)
                                                                                                          tagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTc                                              >  1:794814/13‑120 (MQ=255)
                                                                                                          tagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTc                                              <  2:797378/108‑1 (MQ=255)
                                                                                                           gtattaaaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGa               <  4:287646/145‑1 (MQ=255)
                                                                                                            tatgaaaCTTTCATTGGGTGCTTGGTCCGGAATTGTACCTACGACCTTCGCGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt              <  4:610430/146‑1 (MQ=25)
                                                                                                            gggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGAGTTTTCATGCAACCTTTTTTTTACCCCGATc             >  7:1716285/11‑151 (MQ=255)
                                                                                                            catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc             >  1:502755/1‑151 (MQ=255)
                                                                                                            catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc             >  8:691858/1‑151 (MQ=255)
                                                                                                            catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc             >  1:1708266/1‑151 (MQ=255)
                                                                                                            aatcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCa            <  2:138155/150‑1 (MQ=255)
                                                                                                             atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc             >  2:1790238/1‑150 (MQ=255)
                                                                                                             atcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGTAGGCCCAGTATACTCCGCTCGAGATTTCATTCAACCttattttagataagagc             >  7:929599/1‑139 (MQ=14)
                                                                                                              tcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc           >  4:1574763/1‑151 (MQ=255)
                                                                                                               tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTc                                              <  6:136750/108‑1 (MQ=255)
                                                                                                               cctatcttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc           <  6:1401746/144‑1 (MQ=255)
                                                                                                                gttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGATCTCGTTTTCATGCAACCTTTTTTTTAACCCCATCACgg         >  2:1025453/7‑151 (MQ=18)
                                                                                                                aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  7:158374/1‑151 (MQ=255)
                                                                                                                 ttctatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                            <  5:1422171/126‑1 (MQ=255)
                                                                                                                 aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGa        <  3:673197/151‑1 (MQ=255)
                                                                                                                  tcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGa        <  2:887545/146‑1 (MQ=255)
                                                                                                                  aatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAAGCGGAGTATACTACGCACTGGTTTTCGTGCAGCCTGTTTTTTAGGCCGATAACGGAt       >  2:1142731/3‑151 (MQ=14)
                                                                                                                  aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt       >  5:831408/1‑151 (MQ=255)
                                                                                                                    tttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCg                                                 <  8:269338/107‑1 (MQ=255)
                                                                                                                    tttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     >  7:1396991/1‑151 (MQ=255)
                                                                                                                    aatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     <  5:524474/149‑1 (MQ=255)
                                                                                                                    aatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAACCCGATCACGGAttt     >  4:955560/3‑151 (MQ=255)
                                                                                                                    aatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCGCACTATACTCCGATCGGGTTTTCATGCAACCTTGATTTTACCCACATCCGGGAttt     >  1:715509/3‑151 (MQ=18)
                                                                                                                    aatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACACCGATCGAGATTTCATTCAACCGTTGTTTTACCCCCATCACCGAttt     >  4:1175619/3‑151 (MQ=17)
                                                                                                                     ttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTg    >  4:791190/1‑151 (MQ=255)
                                                                                                                     atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                                           <  3:1679420/111‑1 (MQ=255)
                                                                                                                     atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGGAACCTTTTTTTTACCCCGATc             >  5:1470152/2‑142 (MQ=255)
                                                                                                                     atAATTGGATGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACTATACTCCGCTctcg                                           >  4:1686378/2‑112 (MQ=255)
                                                                                                                      tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTTTGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCTTTTTTTTACCCCGATCACGGATTTgg   >  1:666770/1‑151 (MQ=255)
                                                                                                                      tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTgg   >  2:1201765/1‑151 (MQ=255)
                                                                                                                      tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCGGAGTATACTACGATATAGTTTTCATGGAGCCGTGATTTTAACCCGAGCAGCGATTTgg   >  5:438357/1‑151 (MQ=14)
                                                                                                                      tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCGCACTATACTCCGCTCGGGTTTTCATGCAAGCTTTTTTTTACCCCGATccc           >  5:1401682/1‑141 (MQ=255)
                                                                                                                       aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGc  >  3:891181/1‑151 (MQ=255)
                                                                                                                       |                                                                                                                                                      
CAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936180‑936447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: