Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,169,7200CG26.8% 157.6 / 48.7 161E614Q (GAA→CAA) rsxCelectron transporter RsxC
Reads supporting (aligned to +/- strand):  ref base C (76/36);  new base G (37/4);  total (120/41)
Fisher's exact test for biased strand distribution p-value = 6.24e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

CTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCGCGGGC  >  DH1/2169570‑2169870
                                                                                                                                                         |                                                                                                                                                       
cgtttgctattgCAATAGCGTCTGCAACTGCGGATTTTCGCGTAGCTACCTGATGTTCTGGCTCCGCATTAGCCTGGTGCTGT‑GCGTGGTTGCGT‑GCTTTTGCCCGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGttc                                                                                                                                                         <  4:1094966/140‑1 (MQ=2)
     gctggttctaTAGCCGCTGCCACGGCCGCTTTGCGCGGATCGACCCGTTCTTGTGGACGGGCATTATCCGGTTGGTGT‑GCCAGCTTGCGG‑GCCTTCGCCCGGGCAATAGCGGCTGCCA‑CTGCGGCTTTGCGCGGATCGACCTGTTCTTCTg                                                                                                                                                    <  3:687579/142‑1 (MQ=2)
     gcgcgtgCAATCGCCGCTGCATCGGCGGCTGTCCGCGTAGCGACGTGGTGTTCGGGCTCGGCAATAGCCTGTTGCTGT‑TCCCGCTTGCGG‑GCCTTCGCCCGGGCAATAGCGGCTTCTA‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTg                                                                                                                                                    <  1:880584/145‑1 (MQ=2)
        ctttcAATAGCCGCGGCAACTGCGTCTTTGCGCGGATCTACCTGTTGTTCTGGCTCCTCATTAGGCTGTTGCTGT‑TCCAGTTTGCGT‑GCTTTTGCTCGGGCAATAGCGGCTTCGA‑CTGCGGCTTTGCGCGGATCGACCTGTTCTTCTg                                                                                                                                                    <  8:1130175/144‑1 (MQ=11)
             aatagcctcggccaCGGCCGCTTAGCGAGTATCCACCTGTTCTTCTTGCCCCGCATTAGCCTGTTGCTGTCTCCCG‑TTTCTT‑GCTTTCGCGCGGGCAATAGCGGCTTCTA‑CTGCGGCTTTGCGCGGATCGACCTGTTCCTCTGGTTCCGCa                                                                                                                                            <  8:1478855/138‑1 (MQ=2)
               gcggggccgccaCGGCCGCTGTGCGCGGATCCACCTGTTGTTCTGGCGCCGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCCCGTGCAATAGGGGCGTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCAtt                                                                                                                                          <  7:1327996/140‑1 (MQ=11)
                aGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGGTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCAGGTTTGAGAGGATCTGCATCGTTTTCTGGTTCCgaagtt                                                                                                                                         >  8:503436/1‑146 (MQ=11)
                          actgccggtttGCGCTGATCTATCTGTTGTTATTGCTCGGCATTATCCTGTTGCTGT‑TCGTGGTTTTTT‑GCTTTTGCTCGGGCAATAGCGGCTTCTA‑CTGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGc                                                                                                                                <  8:690566/142‑1 (MQ=14)
                          aCGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGA‑GCTTTGGCACGGGCAATAGCTGCTTCGA‑CGGCGGCTTTGCGCGGATCGCCCTCTGGTTCTGCTTCGTCATTATCCTGTAGCt                                                                                                                               >  1:366895/1‑151 (MQ=11)
                                gcTTTGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCCA‑CGGCCGGTTTTCGAGGATCACCAACTTGTTCTGGTTCCTGATTTTCCTGTTGGTCTTc‑c                                                                                                                         >  1:21733/1‑150 (MQ=11)
                                  tttGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGA‑GCTTTGGCACGGGCAATAGCTGCTTCGA‑CGGCTGATTTGCGAGGATCACCAACTGGTTCTGCGTCCGAATTAGCCTGATGATCTTC‑CAGc                                                                                                                      >  2:1020516/1‑151 (MQ=11)
                                   ttGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCGCGGGCTATAGCTGCTTCGA‑CGGCGGGTTTCCGAGGATCCCCAACGGGTTCTGGTTCCGGATTATCCTGTTGTTGTTC‑CTGCt                                                                                                                     >  5:748383/1‑151 (MQ=11)
                                     gcgcGGATCTAGCTGTGCTGCTGGCTCGTCAGATGGCTGGTGCTGT‑GCC‑GCTTTTGTGGCCTGCGCGCGGGCAATAGCGGCTTCTA‑CTGCGGCTTTGCGCGGATCTACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTC‑CAGCTTg                                                                                                                   <  1:441929/151‑1 (MQ=2)
                                        cgGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCTGCTTCGA‑CGGCGGGTTTGCGGGGATCCCCAACTTGTTCTGGTTCCGAATTATCCTGTTGGTGTTC‑CTGCTTccgg                                                                                                                >  7:130022/1‑150 (MQ=11)
                                          gATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCGCGGGCCATAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGCCCTGGTGTGCTGGTTCCGGAGTATGCTGGTGGTGTTC‑CTGCTTgggggg                                                                                                              >  2:1418049/1‑146 (MQ=11)
                                          aaTCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCAA‑CGGCCGATTTACGGGGATCCCCAACTTGTTCTGGTTCCTTATTATCCTGATGCTCTTc‑ctgattccccg                                                                                                               >  3:153337/2‑140 (MQ=2)
                                               aCCTGTTGTTCTTGCTCTGTTATATCCTTGTGCTGG‑TCGTGCTTTCTT‑GCGTTTGCTCGGGCAATAGCCGCTGCTA‑CTGCGGCTTTGCGCGGATCTACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCt                                                                                                             <  6:575327/147‑1 (MQ=9)
                                               aCCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCCATAGCGGCTTCGA‑CGGCGGCTTTGCGGGGATCGCCCTCGGGTTCTGCTTCCGGATTTGCCTGTTGGTGTTC‑CAGCTTgcgtcctgtgg                                                                                                         >  5:1591752/1‑143 (MQ=11)
                                                 cTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCCCGGGCAATAGCTGCTTCCA‑CGGCAGGTTTTCGGGGATCGGCAACGTTTTCTGCTTCCTCATTATCCTGTTGATCTGC‑CTGCTTGAGAGCtgtcg                                                                                                         >  1:1282894/1‑145 (MQ=2)
                                                 cTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCTGCTTCGA‑CGGCGGGTTTGCGCGGATCGCCCTGTTGTTCTGGTTCCGGATTAGCCTGTTGCTGTTC‑CAGCTTGCGAGATTTgtcc                                                                                                       >  4:1519200/1‑148 (MQ=11)
                                                 cTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCGGATTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTATCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGcc                                                                                                       >  7:698959/1‑150 (MQ=255)
                                                  acctatccTGGCTCGTGATGAGCCTGCTGCTGG‑TCGGGTTTTCTG‑GCCTGAGCGCGGGCAATAGCCGCTGCAA‑CTGCCGCTTTGCGCGGATCCACCTGTTCTTCTGGCTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGTGCTTTGGCAc                                                                                                      <  3:813148/144‑1 (MQ=17)
                                                      gttCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCGGGTTTGCGGGGATCGGCATGTTGTTCTGGTTCCGAATTTTCCTGTTGCTGTTA‑CAGCTTGAGCGCTTTGGCGCTGGc                                                                                                  >  1:488919/1‑151 (MQ=11)
                                                       ttCTGGTACCGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCGCGTGCAATAGCCGCTTCGA‑CGGCAGCTTTGCGCGGATCGACCTGTTATATGGGATCCGCATAAGGCTGTACTACTTA‑CAGATTGAGAGCTTTGGGACgggg                                                                                                  >  1:649562/1‑149 (MQ=9)
                                                         cTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGA‑GCTTTGGCACGGGCAATAGCTGCTTCAA‑CGGCAGGTTTACGAGGATCAACCACGTGTTCTGGTTCCGAATTTTCCTGATGATCTTC‑CAGATTCCGACCttttacaacggaaa                                                                                                >  6:686944/1‑139 (MQ=2)
                                                          gggCTCGGCTTCAGCCTGGTGCTGT‑TCCCGCTTTCGG‑GCGTGGGCGCGGGCAATAGCCGCTGCTA‑CTGCCGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATa                                                                                              <  5:1800069/150‑1 (MQ=14)
                                                          cgcgcagcctttctccTGGTGCTGT‑TCCCCTTTTTGG‑GCGTTTGCTCGGGCAATAGCCGCGTCTA‑CTGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGTGCTTTGGCACGGGCAATa                                                                                              <  8:1317782/137‑1 (MQ=14)
                                                            gcacGGCATTATCCTGTTTCTGG‑GCCAGCTCGCGT‑TCTTTCTCACTGTCTATACCGGTTTCGA‑CCGACGACTTGCTAGGTTCCACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGc                                                                                            <  8:1321100/148‑1 (MQ=9)
                                                            gCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCTCGGGCGATAGCGGCTTCGA‑CGGCGGCTTTGCGGGGATCGCCCTGTGGTGCTGGGTCCGGAGTAGCCTGTTGCTGTTC‑CAGGTTGCGCGCGTTGGCCCggggactcgc                                                                                            >  5:183629/1‑144 (MQ=11)
                                                                 gCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCGCGTGCAATAGCCGCTTCGA‑CGGCAGATATCCGAAGATCGACCTGTACTTAAAGATCCGCAATAGAAGGTTACTTTTC‑CCGTTTGCGCGCTTTGGGAATTTAAATAGCGGCtt                                                                                       >  8:1383737/1‑151 (MQ=9)
                                                                   aTTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCGCGTGCAATAGCGGATTCGC‑AGGCGTCTTTGCGCCAGTCACAGTCTTCATCTGGGTCGGCATTAGGCTGGTTCTGATC‑AAGATTGCGCGCTTTGGGGCGGGCGAGAGCgggggcg                                                                                     >  7:730697/1‑146 (MQ=2)
                                                                        ccTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCAA‑CGGCAGATTTGCGAGGATCGGCCAGGGGTGCTGGTTCGGCAGTTGCCTGTAGGTGTTC‑CAGCTTCCCCCATTTGGCACCGGTAATAGcttctactcctgc                                                                                >  5:507688/1‑139 (MQ=2)
                                                                          tGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCTGCTTCGA‑CGGCGGATTTGCGAGGATCGACCTCTTGTTCTGGTTCCTAATTATCCTGATGATGTTC‑CAGATTCCGAGATTTTTCACGTGAAATAgctgctgctactgca                                                                               >  4:564956/1‑141 (MQ=2)
                                                                          cgggcgctgccgcAGCTTGCGG‑GCCTGGGCGCGGGCAATAGCCGCTTCCA‑CGGCCGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGAcggcg                                                                               <  1:480373/139‑1 (MQ=14)
                                                                           gTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCGCGGGCCATAGCTGCTTCGA‑CGGCGGGTTTGCGCGGATCGCCCTGTTGTTCTGGTTCCGGAGTATCCTGTTGCTGTGC‑CAGCTTGCGCGGGTTGGCCGGGGGAATTGCGGCTTCCAcgcctgt                                                                             >  3:161171/1‑146 (MQ=14)
                                                                             tGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCGGGTTTGCGCGGATCGCCCTGTTGTTCTGGTTCCGCAGTATCCTGTTGGTGTTC‑CAGCTTGCGCGCTTTGGCCCGGGCAATAGCGTCTTCTCcggcaggtt                                                                           >  1:107744/1‑146 (MQ=11)
                                                                               cTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGAGGATCGACCTCTTGTTCTGGTTCCTGATTAGCCTGTTGATGTTC‑CTGCTTGCGCTCTTTGGCGCGGGCAATAGCGGCTTCGAGGGCCGCTTTg                                                                         >  6:1466247/1‑151 (MQ=11)
                                                                                    tCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCTGCTTCGACGGCAGCTTTGcgcg                                                                     >  2:308793/1‑151 (MQ=255)
                                                                                      cAGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCCCGGGCAATAGCGGCTTCAACGGCCGATTTCCGAgg                                                                    >  3:549765/1‑150 (MQ=11)
                                                                                       aGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGCCCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCGCGGGCAATAGCGGCTTCGCCGGCGGGTTTGCGCGGAt                                                                  >  5:609563/1‑151 (MQ=255)
                                                                                       aGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTAGTTATGGGTCCGCATTAGACTGTTGCTGTTG‑CAGCTTGCGCGGTTTGGCACGGGCGATAGCGGCTTCGAcgggggggtggggggggg                                                                  >  2:872550/1‑139 (MQ=11)
                                                                                        gCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGGGGATCGCCCTGTTGTTCTGGTTCCTGATTAGCCTCTTGCGGTTC‑CAGCTTGCTCGCTTTGGCACGGGCAATAGCTGCTTCCCGGGCCGCTTTTCGGGGATc                                                                 >  3:844588/1‑151 (MQ=11)
                                                                                         tattgataggtgtGGCACGGTCATTACCTGATTCGT‑CGTCTGTTTTGCGCTCATCGACGTTCTTTTGATCTTCGGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCACGGAt                                                                  <  8:871399/138‑1 (MQ=2)
                                                                                         cTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGGGGATCGCCCTGTTGTTCTGGTTCCGCATTATCCTGTTGATGTTC‑CAGCTTGCGAGCTTTTGCACGGGCAATAGCTGCTTCGACGGCGGGTTTTCGTGGATcc                                                                >  1:1134384/1‑150 (MQ=11)
                                                                                           tGCGT‑GCTTTGGCTCGGGCAATAGCGGCTTCGA‑CTGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGa                                                               <  4:1838559/150‑1 (MQ=255)
                                                                                           ccgcgcgctcgggCCCGGGCAATAGCGGCTTCGA‑CGGCAGCCTTGCGCGGAGCTACCTGATCTGCTCGTGCCGCAGGAGCCGGGTGCTGTTC‑CCGCTGGCGCGCTTTGGCCCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGa                                                               <  3:556698/140‑1 (MQ=2)
                                                                                               gcgcgggggCCCGCGCACTCGCGGCTTCGA‑CTGCCGCTTGCCGCGGCGCGACCTGTTCTCCGGCTGCCCCAGGAGCCGGGAGGTGTGC‑CCGCCGGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCtg                                                           <  5:1459168/144‑1 (MQ=2)
                                                                                                   tttGGCACGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCTGCTTCGACGGCCGATTTGCGAGGATCGACCtcgtgt                                                       >  7:359771/1‑146 (MQ=11)
                                                                                                     tGGCACGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGCCCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTTCGAGGATcccccacgtgttc                                                     >  1:1248582/1‑139 (MQ=14)
                                                                                                          cGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCTACGGCGGCTTTGCGAGGATCGCCCTGTTGTTCTGGtt                                                >  8:1003038/1‑151 (MQ=255)
                                                                                                           cggCAATAGCTTCTTCAAGCGGCGG‑TGTGCTCTGATCGTCCGTTTCTTCTGGGTGAGGAGTTTCGTGTTGCTTTTC‑GATCTTTTGTGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTc                                               <  5:1144772/150‑1 (MQ=2)
                                                                                                            ggggactgccgCTGCGA‑CGGCGGCTCTGCGGCGCTCCACCTGTTCTTCTGGCTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTcc                                              <  7:860540/141‑1 (MQ=11)
                                                                                                             tcAATAGATGCGTCGA‑CCGCCGCTTTGCTCTGATCGACCTCGTCTTCTGGCTCCGAATAAGCGGTTGGCTGTTC‑CAGCTTGCGTGCTTTGGCTCGTGCAATAGCGGCTTCGACGGCTGCTTTGCGCGGATCGACCTGTTGTTCTGGTTcc                                              <  8:194702/149‑1 (MQ=2)
                                                                                                             ccccgaGCGGCGGCGA‑CGGCCGCTTTGCTTTTATCAATGTGTGCTGCTGCCCCGGCATTACCATTTTGCGGCTC‑CCGCTTGCGCTCGTCGTCTCGGTTAAGAGCTGCTTCGACGGCCGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCg                                             <  8:1407368/146‑1 (MQ=2)
                                                                                                              cAATAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCCCGGGCAATAGCTGCTTCTACGGCTGGTTTTCGAGGATCTGCCTGGGGTTCTGGTTcggt                                            >  7:1711553/1‑148 (MQ=11)
                                                                                                               aaTATCCGCGTCGA‑CGGCCGCTTTGCTCGTATCACCCGCATCTTCTTGCTCTCCACTAGCCTGTT‑CCGTTCTCAGCTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCa                                           <  6:739265/151‑1 (MQ=2)
                                                                                                               aaTAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGGCTGTTGCTGTTC‑CAGCTTGCGCCCTTTGGCACGGGCAAAAGCGGCTTCCACTGCTGTTTTGCGAGGATCGACCAGGTGTTCTGGGTCCgg                                            >  1:732039/1‑149 (MQ=14)
                                                                                                                aTAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGATTTGTCCCGGGCAATAGCTGCTTCTACGGCAGGTTTGCGCGGATCAGCCTCGTGTTCTGCTTCCGTAt                                          >  4:95601/1‑151 (MQ=11)
                                                                                                                  aGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCCCGGGCAATAGCGGCTTCAACGGCGGCTTTACGGGGATCAGCCGGGGTTTCTGGTTCGGTAttt                                        >  8:207686/1‑150 (MQ=1)
                                                                                                                  aGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCGATAGCGGCTTCCACGGCGGCTTTTCGAGGATCGGCCTCGGGTTCTGCTTccttagtt                                        >  1:1726040/1‑145 (MQ=14)
                                                                                                                    cGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCCCGGGCAATAGCTGCTTCGACGGCAGGTTTTCGAGGATAATCCTGGGGTTCTgcgtcgtgattatg                                      >  2:161612/1‑138 (MQ=11)
                                                                                                                      gCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCt                                    >  8:726053/1‑151 (MQ=40)
                                                                                                                        ttCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGGCATGGTGTGCTGGTTCCGCATTATCCTGt                                  >  3:1780697/1‑151 (MQ=255)
                                                                                                                           gA‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCTCGGGCTATAGCGGCTTCGACGGCTGGTTTGCGGGGATCGTCCTGTTGTTCTGGTTCGGTAGTAGGCTGtttc                               >  5:1775033/1‑149 (MQ=14)
                                                                                                                            a‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGCGCAATACCGGCTTCGACGCCAGCATTACGAGGATCAACAACTTTTTCTGCGTCCGGAGTATCCTga                                  >  2:701943/1‑146 (MQ=11)
                                                                                                                              cggcggCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCGATAGCGGCTTCGACGGCGGCTTTGCGAGGATCGACCAGTTGTTCTGCTTCCGAATTATCCTGTTGATg                             >  4:1795305/1‑151 (MQ=14)
                                                                                                                               ggcggcTTTGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGTGCTTTGGCACGGGCGATAGCTGCTTCAACGGCAGATTTGCGAGGATCGACCACTGGTTCTGCGTCCTAATTATCCTGATggtct                            >  2:125931/1‑147 (MQ=11)
                                                                                                                               ggcggcTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCCCGGGCAATAGCGGCTTCGACGGCAGCTTTGCGAGGATCGCCAACGGGTGCTGGTTCCGGATTATGCTGTAGCTGt                            >  2:302723/1‑151 (MQ=14)
                                                                                                                               ggcggcTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGCCCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGt                            >  3:1209865/1‑151 (MQ=255)
                                                                                                                               gccatcgttGCCCGGATGTACCTTTGCTTATGGTTCTGCTATAGCCTGTTGCTGTTC‑CAGCTTGCGTGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCTGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGt                            <  8:462873/144‑1 (MQ=11)
                                                                                                                                gcggcTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCTGCTTCGACGGCGGCTTTTCGAGGATCGACCTGGTTTTCTGGTTCCGGATTATCCTGTTGGTGtt                           >  6:989116/1‑151 (MQ=14)
                                                                                                                                 cggcTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCGCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGAGGATCGCCCTGTGGTTCTGGTTCGGCTTTAGCGTGtttgtgtgc                          >  4:422582/1‑144 (MQ=14)
                                                                                                                                 cggcTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCGATAGCGGCTTCAACGGCGGATTTACGAGGATCGACAACTTTTTCTGCTTCGGGATTTGCCTGGAGATGTTc                          >  6:1048488/1‑151 (MQ=11)
                                                                                                                                  ggcTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACATGTTGTTCTGGTTCCTCATTATCCTGTTGATCTTcc                         >  7:1282664/1‑151 (MQ=255)
                                                                                                                                  ggcTTTGAGCTTATCTACGTGGTCTTCTGGTTCCGCATTAGCCTGTTGTTGTTG‑CTGCTTGCGCGCTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTcc                         <  4:1220788/151‑1 (MQ=14)
                                                                                                                                    cTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCCGATCGCCCTGGGGTTCTGGTTCCGCATTAGCCTGTTGATGTTcc                         >  5:19348/1‑149 (MQ=255)
                                                                                                                                     tttGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGAGCTTTGGCCCGGGCAATAGCTGCTTCAACGGCAGGTTTACGTGGATCACCAACGTGTGCTGGTTCCTAATTATCCTGATGATCTTcctgt                      >  6:1115268/1‑148 (MQ=11)
                                                                                                                                     tttGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTCC‑CAGCTTGCGCGCTTTGGCGCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGGGGATCGCCCTGTGTTTCTGGTTCCGCATTAGCCTGTTGGTGTTCCAgg                      >  1:143479/1‑150 (MQ=14)
                                                                                                                                      ttGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCTGGTTTGCGCGGATCAGCCTCTGGTTCTGCGTCCGAAGTAGCCTGAAGCTCTTCCAGCt                     >  4:738274/1‑151 (MQ=14)
                                                                                                                                         cgcgGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCTGCTTCGACGGCGGCTTTGCGAGGATCGACATCTTGTTCTGCTTCCGAATTATCCTGTTGATCTTCCAGAttcc                  >  6:1867845/1‑149 (MQ=14)
                                                                                                                                         cgcgGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTgc                  >  7:1585031/1‑151 (MQ=40)
                                                                                                                                         cgcgGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCCGGTATACGCGGATCGCCAACGTGTTCTGCTTCCGAATTATCCTGGAGTTGTTcctggttcc                  >  6:1274533/1‑144 (MQ=14)
                                                                                                                                          gcgGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCGCGGGCAATAGCTGCTTCGACGGCGGGTTTCCGGGGATCAGCAACTGGTGCTGCTTCCTAAGTATCCTGATGGTGTTCCAGATTccg                 >  5:474792/1‑151 (MQ=2)
                                                                                                                                           cgGATCGACCTGCTCGTCTGGGGCCTCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTgcgc                <  8:102876/151‑1 (MQ=255)
                                                                                                                                            ggATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCCCGGGCGATAGCGGCTTCGACGGCGGGTTTGCGCGGATCGCCAAGTTGTTCTGGTTCCTCATTATCCTGTTGGTGTTCCAGGTTgcgcg               >  7:736199/1‑151 (MQ=14)
                                                                                                                                             gATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCTGCTTCGACGGCGGCTTTGCGCGGATCGGCCTGTTGTTCTGGTTCGGAATTATCCTGTTGGTCTTCCAGCTTgcgcgg              >  3:1653749/1‑150 (MQ=14)
                                                                                                                                              aTCGACCTGTTCTTCTGGTTCCCCATTAGCCAGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCCCGCGCAATACCGGCTTCGACGGCAGCTTAGCGAGGATCAACCACTGGTACTGGTTCCTAATTAGCCTCAAGCTGTTCCAGATTccgacat             >  8:1292492/1‑147 (MQ=12)
                                                                                                                                              aTCAACCTGTTCTTCTGGTACCGCATTAGCCTGTTGCTGTTC‑AACCTTCAGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCAGCTTTGCGCGGATCAACATGTTCTTCTGGGTCCGCATTAGCGTGGGGCTGtggccgggtgggggct             >  7:1775058/1‑136 (MQ=17)
                                                                                                                                               tCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCtt            >  3:284163/1‑151 (MQ=40)
                                                                                                                                                cGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCTGCTTCGACGGCAGATTTGCGAGGATCGCCCTGTTGTTCTGGTTCCGCATTAGCCTGATGATGTTCCAGATTGCGCGAttt           >  5:691041/1‑151 (MQ=14)
                                                                                                                                                cGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCttt           >  3:597040/1‑151 (MQ=40)
                                                                                                                                                  aCCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCGCGGGCGATAGCGGCTTCGACGGCGGGTTTGCGAGGATCAGCCAGTTGTTCTGGTTCGGCATTATCCTGTTGGTCTTCCAGGTTgcgagcgtgtg         >  2:467284/1‑146 (MQ=14)
                                                                                                                                                    cTGTTCTTCTGGTACCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCTCGGGCAATAGCCGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGt                                                 <  7:1554609/109‑1 (MQ=255)
                                                                                                                                                     tGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCTGCTTCGACGGCGGGTTTGCGAGGATCGCCCTGTTGTTCTGGTTCCGAATTAGCCTGATGATCTTCCAGATTccgacagtatgccc      >  4:1146471/1‑140 (MQ=14)
                                                                                                                                                     tGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGGTTTGCGGGGATCGCCCTGGTGTTCTGGTTCCGCAGTAGCCTGTTGGTGTTCCAGCTTGCGGGCTTTGtcgc      >  7:1445925/1‑151 (MQ=14)
                                                                                                                                                      gTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGAATTATCCTGTTGGTGTTCCAGCTTGCGAGATTTGGcgcg     >  1:592678/1‑151 (MQ=255)
                                                                                                                                                        tcttctGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCGCggg   >  3:253806/1‑151 (MQ=255)
                                                                                                                                                        tcttctGGTTCCGCATTAGACTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCCCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGAGGATCGACCGGGGGTTCTGCTTCCGGATTAGCCTGTTGCTCTTCCAGCTTGCGCCCGTGTGCGCggt   >  6:225199/1‑150 (MQ=14)
                                                                                                                                                        tcttctGGTACCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGTGCTTTGGCGCGTGCAATAGCCGCTTCGACGGCGGCTTAGCTCGGATCAGCCTCTTGTTCTGGATCAGAATTACGCTGTTTGTGTTACAGGTTGCTCGCGTTTgcaagt    >  2:1039892/1‑146 (MQ=2)
                                                                                                                                                         cttctGGCTCCGCATTAGCCTGTTGCTGTTC‑CAGGTTGAGATCTTAAGATCGTCCAATAGCGAATTAGACTGCGTCTTTGAGATGATCTACCTGTTCTGCTTTTTAATAAAAATAATGTTGCTGTTCCAGCTTGCGTGCGTTGGAGCgggg  >  2:140341/1‑150 (MQ=2)
                                                                                                                                                         |                                                                                                                                                       
CTTTGGCTCGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGCTCGGCATTAGCCTGTTGCTGT‑TCCAGCTTGCGT‑GCTTTGGCACGGGCAATAGCGGCTTCGA‑CGGCGGCTTTGCGCGGATCGACCTGTTCTTCTGGTTCCGCATTAGCCTGTTGCTGTTC‑CAGCTTGCGCGCTTTGGCACGGGCAATAGCGGCTTCGACGGCGGCTTTGCGCGGATCGACCTGTTGTTCTGGTTCCGCATTAGCCTGTTGCTGTTCCAGCTTGCGCGCTTTGGCGCGGGC  >  DH1/2169570‑2169870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑