Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,326,2870GT23.9% 988.2 / 78.9 462intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
Reads supporting (aligned to +/- strand):  ref base G (234/106);  new base T (18/89);  total (253/209)
Fisher's exact test for biased strand distribution p-value = 7.53e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 466 total aligned reads displayed.

GATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATAT  >  DH1/2326137‑2326436
                                                                                                                                                      |                                                                                                                                                     
    cgcTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGATATCATAACAGATAAGTCTTTCAAAACGCATACCGAATAAAGAAAGCAAACGTTGTGAACAATATGAAAATAAAAAATATTACAGCGCCATTTGaa                                                                                                                                                     >  2:1301960/1‑149 (MQ=2)
       tACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACtg                                                                                                                                                 >  4:207768/1‑150 (MQ=18)
        aCGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtga                                                                                                                                                <  3:1574841/150‑1 (MQ=38)
          gCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACttaaa                                                                                                                                              >  6:245474/1‑146 (MQ=14)
          gCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtgatga                                                                                                                                             <  5:1657540/151‑1 (MQ=39)
          gCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACtgatg                                                                                                                                              >  4:1622375/1‑150 (MQ=39)
             tATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACttatgaaa                                                                                                                                           >  2:848738/1‑148 (MQ=16)
             tATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTTATAACa                                                                                                                                           >  2:594220/1‑150 (MQ=16)
              aTCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACaa                                                                                                                                          >  5:784711/1‑150 (MQ=255)
              aTCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACaaa                                                                                                                                         <  6:1029349/151‑1 (MQ=255)
              aTCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACaa                                                                                                                                          <  1:331143/150‑1 (MQ=255)
                cAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAAc                                                                                                                                        <  1:1074099/150‑1 (MQ=255)
                 aGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTTATGATaaaaa                                                                                                                                       >  3:947409/1‑148 (MQ=255)
                 aGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGAAGACAAACGc                                                                                                                                      >  5:1867160/1‑151 (MQ=255)
                  ggCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCa                                                                                                                                     <  4:852472/151‑1 (MQ=255)
                     cTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGCCGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAAc                                                                                                                                   >  5:1211836/1‑150 (MQ=255)
                      tACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACAt                                                                                                                                 >  7:1215996/1‑151 (MQ=255)
                         acGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGAAAACAAAAAAAACAtta                                                                                                                               >  2:964600/1‑149 (MQ=11)
                          cGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACATCGCAATATCAAACTGAAGACAAACGCAAAAtttc                                                                                                                              >  6:1607373/1‑148 (MQ=14)
                           gTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTTATGACAAACGCAACATTGCCt                                                                                                                            >  5:1773153/1‑151 (MQ=255)
                             tCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGACTg                                                                                                                           >  4:524985/1‑150 (MQ=255)
                              cagtggattgtATTGGATTTGAAGGGTTTTGATGCCGTTTATGTGTTTAACGACGCATTCTGCATGAACAAAGCCTTCGTTGTATACAATATTAAAATGTCGCTTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGAt                                                                                                                         <  2:1119225/141‑1 (MQ=19)
                              cACTGCAATCTATTGGATTTTAAGGGTTTTCATGCCGTTTATGGCGTTCACGACGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGa                                                                                                                          <  2:1433241/150‑1 (MQ=17)
                                 tgaaaTCTATTGGATATGAAGGGTTTTCATGCACGATAAGGCGTTCACGCCCCAACCGGCATGAACAAAGCCTACGTTATCTACAATATGAAAATGACGACTTTTACAGCGCCATATTAAACTGATGACAAAAACAACATTACatgatgac                                                                                                                      >  6:1518292/4‑149 (MQ=14)
                                  gCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATgcgc                                                                                                                     >  8:1341315/1‑151 (MQ=255)
                                   cAATCTATTGGATGTGAAGGGTGTTCATGCCGTATATCTCGTTCAAGCCGCATCCTGAATGAACAAAGCCTACGTTGTCTACAATAAGAAAATGACCCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCt                                                                                                                    <  5:324930/151‑1 (MQ=18)
                                    aaTCTATTGGATTGGACGGGTTTTCAAGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTa                                                                                                                   <  3:570863/151‑1 (MQ=255)
                                           tGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTAt                                                                                                            >  8:1555334/1‑151 (MQ=255)
                                            ggATTTGAAGGGGTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATc                                                                                                           <  5:460397/151‑1 (MQ=255)
                                              aTTTGAAGGGTTTTCATGCCGTATAAGTCTTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATTAAAATGACTCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCa                                                                                                          <  4:1372734/150‑1 (MQ=18)
                                              aTTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAg                                                                                                         <  7:502477/151‑1 (MQ=255)
                                                   aaGGTTTTTCATGCCGGTTTAGTCTTTCACGCCGCATCCGGGATGAACAAAGCCTACTTTGTCTACAATATTAAAATGACTCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCt                                                                                                     <  2:486659/150‑1 (MQ=25)
                                                     gggTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTaca                                                                                                  <  3:502252/151‑1 (MQ=255)
                                                      ggTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTacac                                                                                                 <  1:632049/151‑1 (MQ=255)
                                                       gTTTTCATGCCGGATAAGGCGTTCACGCGGGATGCGGCATGAACAAAGCCTTCGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCACCATTGCCTGATGCGCTACGCTTATCAGGCCTACACg                                                                                                <  8:1426748/151‑1 (MQ=21)
                                                       gTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACg                                                                                                >  1:200091/1‑151 (MQ=255)
                                                           tCATGGCGGCTCAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGCCGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTc                                                                                             <  1:235006/150‑1 (MQ=255)
                                                             aTGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCAcg                                                                                          >  3:1140753/1‑150 (MQ=255)
                                                              tGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTg                                                                                         >  1:435889/1‑151 (MQ=255)
                                                               gCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGc                                                                                        >  1:765393/1‑151 (MQ=255)
                                                               gCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGc                                                                                        >  8:64640/1‑151 (MQ=255)
                                                                ccGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGAAAAACGCAACATTGCCTCAAGCGATACGCATATCATGCCTCAACATTCACTGc                                                                                        >  6:1797232/1‑150 (MQ=18)
                                                                 cGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCa                                                                                       >  5:1001714/1‑150 (MQ=255)
                                                                 cGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCGTATCAGGCCTACACGTTCACTGCa                                                                                       >  1:93403/1‑150 (MQ=255)
                                                                     tAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCt                                                                                   >  5:1389927/1‑150 (MQ=255)
                                                                      aaGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACTTTGTATACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTa                                                                                  <  7:2505/150‑1 (MQ=255)
                                                                      aaGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTAt                                                                                 >  7:981092/1‑151 (MQ=255)
                                                                       agtcGTTCAGGTCGTATACGGAATTAACAAAGCCTACGTTTTATACAATATCAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTAtt                                                                                <  3:217824/148‑1 (MQ=18)
                                                                        ggCGTTCACGCCGCATCCGGCATGAACAAATCCTACGTTTTCTAGAATATGAAAATTACTCCTTTTACAGCGCCATTTTAAACTTATGACAATCGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTg                                                                               <  8:254696/151‑1 (MQ=21)
                                                                         gCGTTCACGCCTCATCCGGCATGAAGAAAGCCTACTTTGTCTACAATATTAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGGTGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTgg                                                                              <  4:286527/151‑1 (MQ=21)
                                                                            ttCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAtt                                                                           >  8:829228/1‑151 (MQ=255)
                                                                            tacacgagtctttcGGCATGAAGAAAGCCTACGTTGTATACAATATTAAAATGTCTCCTTTTAAAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAt                                                                            <  6:1188268/137‑1 (MQ=17)
                                                                             tcactcatattCCGTCATGAATAAAGTCTACGATGTTTACAATATGAAAATGTCTCTTTTTACAGCGCCATTTTTAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAtt                                                                           <  2:333472/140‑1 (MQ=17)
                                                                             tCACCCCGCATCCGGCATGAACAAAGCCTACGTGGTCTACAATATCAAAATGCCCCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAtt                                                                           <  3:1395122/150‑1 (MQ=21)
                                                                              cACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATTAAAATGACTCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGAttt                                                                          <  4:610201/150‑1 (MQ=255)
                                                                               ccgccgcCTCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTATACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGa                                                                        <  1:1322895/150‑1 (MQ=255)
                                                                                 gccgcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGa                                                                        >  3:1292208/1‑149 (MQ=255)
                                                                                 gccgcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGACTGATGAGCTACGCTTATCAGGACTACACGTTAACTGCAATCTATTGGATTTGa                                                                        >  6:773257/1‑149 (MQ=255)
                                                                                   cgcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCTCTACGATTGTAAGGCCTACACGTTCACTGCAATCTATTCGATTTGAAggg                                                                    >  5:145119/1‑151 (MQ=255)
                                                                                    gcATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTATACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGt                                                                   <  7:750454/151‑1 (MQ=255)
                                                                                     gattgggaaTGAACATAGCATAAGTTGTCTTCATTTCTAAAATGTCCCCTTTTAAAGCGCAATTTTCAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGtt                                                                  <  3:1730456/143‑1 (MQ=11)
                                                                                       cccGGCATGAACAAAGCCTACCTTCTCCCCACACTCAATATCACTCCTTATACAGCCCCATTTTAACCTTACCCGACTCTCAACCTAGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGtttt                                                                <  6:1475633/150‑1 (MQ=2)
                                                                                            cATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAAAGAAACATTGACTGATGAGCTACGAGTATCATTACTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCAtt                                                            >  1:1495306/1‑149 (MQ=21)
                                                                                             aTGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGAAAAAAGCAACATTTCCTGATGAGCTACCATTATCAGTACTACACGTTCACTGCAATATATTGGAATAGAAGGGTTTTCATGcc                                                          >  4:752581/1‑151 (MQ=25)
                                                                                               gAACAAAGCCTACGTTGTATACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCg                                                         <  1:1288852/150‑1 (MQ=255)
                                                                                               gAAAAAAGCCTACTTTGTCTACAATAATAAAATTACCCCTTTTACAGCGCCATTTGAAACTTATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCg                                                         <  5:1511696/150‑1 (MQ=17)
                                                                                                   aaaGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATa                                                     >  6:982388/1‑150 (MQ=255)
                                                                                                      gCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGc                                                 >  5:702179/1‑151 (MQ=255)
                                                                                                       ccTACTTTTTATACAATATGAAAATGACGCCTTTTACAGCGCCATTTTAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCg                                                <  3:884732/151‑1 (MQ=255)
                                                                                                          aCGTTGTCTACAATATGAAAATGACTCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGt                                               <  5:602567/149‑1 (MQ=255)
                                                                                                           cGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTc                                             >  8:498652/1‑150 (MQ=255)
                                                                                                                   aCAATATGAAAATGACGCCTTTTACAGCTCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCAt                                    <  8:1036060/151‑1 (MQ=255)
                                                                                                                   aCAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCAt                                    >  5:1588072/1‑151 (MQ=255)
                                                                                                                   aCAATATGAAAATGACGCCTTTTACAGCCCCATTTGAAACTTATGACAAACGCAAAATTCCCTGATGCGGTACGCTTATAAGGCGTACACTTTAACTGCAATATATTGTATTTCAAGGTTTTTAATGCCGCATAATGCGTTAACGCCGCAt                                    >  1:1004911/1‑151 (MQ=255)
                                                                                                                      atatGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCg                                 >  8:1692831/1‑151 (MQ=255)
                                                                                                                      atatGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCg                                 >  7:642323/1‑151 (MQ=255)
                                                                                                                       tatGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCgg                                >  8:116235/1‑151 (MQ=255)
                                                                                                                          gAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                             >  6:356897/1‑151 (MQ=255)
                                                                                                                           aaaaTGCCGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATg                            <  1:1121517/151‑1 (MQ=255)
                                                                                                                           aaaaTGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                             <  2:1663019/150‑1 (MQ=255)
                                                                                                                           aaaaTGACGCATTTTACAGCGCAATTTGAAACTGATTACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                             <  2:1779503/150‑1 (MQ=255)
                                                                                                                             aaTGACGCCTTTTACAGCTCCATTTGAAACTGATTACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa                          <  2:757340/151‑1 (MQ=255)
                                                                                                                               tGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAc                         >  3:1496486/1‑150 (MQ=255)
                                                                                                                                  cGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACaa                       >  8:1816239/1‑149 (MQ=255)
                                                                                                                                     gttttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCt                  <  1:1217645/150‑1 (MQ=38)
                                                                                                                                     cTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCt                  >  5:1135645/1‑151 (MQ=39)
                                                                                                                                       tttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTa                 <  7:111332/150‑1 (MQ=38)
                                                                                                                                       tttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTAc                >  2:190846/1‑151 (MQ=37)
                                                                                                                                       tatACAGCGCCATTTAAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCt                  <  1:1600423/147‑1 (MQ=11)
                                                                                                                                        ttACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACg               >  4:168341/1‑151 (MQ=37)
                                                                                                                                         tACAGCGCCATATGAAACTGATGACAAACCCAACATTGCCTGATCCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAACGGTTTTCATGCCGGATAAGGCGTTCACGCCCCATCCGGCATGAACAAAGCATACGt              >  4:397153/1‑151 (MQ=11)
                                                                                                                                          aCAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGt              <  7:1541168/150‑1 (MQ=35)
                                                                                                                                           cAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTg            >  3:7385/1‑151 (MQ=34)
                                                                                                                                              cgcCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCt         >  2:1189844/1‑151 (MQ=33)
                                                                                                                                              cgcCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGAATGAACAAAGCCTACGTTGTCt         <  7:1450075/151‑1 (MQ=18)
                                                                                                                                                  aTTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACa      >  6:677631/1‑150 (MQ=33)
                                                                                                                                                  aTTTGAAACTCATGACAAACGCAAAATTGCCTCATGCGCTACGCTTATCAGTCCTACACGTTCACTGCAATCTATTGGATTAGAAGGGATTTCATGCCGGATAAGGCGTTCACGCCGCATCCGCCATGAACAAAGCCTACGTTGTCTACa      >  5:929734/1‑150 (MQ=11)
                                                                                                                                                   tttGAAACTGATGACAAACGCATCATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAat    >  5:1359698/1‑151 (MQ=18)
                                                                                                                                                    ttGAAACTGATGACAAAAGCAACAATGCCTGATGCGCTACGCTTATCAGGCCTTCACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCTGCATCCGGCATGAACAAAGCCTACGTTGTCTACAat    <  7:1644301/150‑1 (MQ=255)
                                                                                                                                                     tGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAatat  >  4:303364/1‑151 (MQ=31)
                                                                                                                                                      |                                                                                                                                                     
GATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATATGAAAATGACGCCTTTTACAGCGCCATTTGAAACTGATGACAAACGCAACATTGCCTGATGCGCTACGCTTATCAGGCCTACACGTTCACTGCAATCTATTGGATTTGAAGGGTTTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCCTACGTTGTCTACAATAT  >  DH1/2326137‑2326436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑