Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,591,2610CA26.2% 719.8 / 144.5 388intergenic (+27/+233)ECDH1_RS12810/ECDH1_RS12815tRNA‑Tyr/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (135/149);  new base A (51/50);  total (188/200)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 403 total aligned reads displayed.

CACCATCTCAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACC  >  DH1/2591111‑2591411
                                                                                                                                                      |                                                                                                                                                      
  ccaTCTCAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCa                                                                                                                                                       >  7:1427153/1‑150 (MQ=255)
  ccaTCTCAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCa                                                                                                                                                       >  1:362459/1‑150 (MQ=255)
   caTCTCAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAAc                                                                                                                                                     >  1:1323127/1‑151 (MQ=255)
   aaTCTCAAGCCTTACCTCAAAATTTGAATTAGCGCTTGTGGGGTTCCCGAGCGGCCTAAGGGAGCAGACTGTAAATCTGCCGTGATCGACTTCGAATGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAAc                                                                                                                                                     <  2:1458030/150‑1 (MQ=21)
      ctcAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGa                                                                                                                                                   >  5:1793377/1‑150 (MQ=255)
      ctcAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCACTTACCTTCCCAAcca                                                                                                                                                   >  7:1593033/1‑148 (MQ=255)
       tcAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAAt                                                                                                                                                 >  7:1200101/1‑151 (MQ=255)
       tcAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGAAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACAACCATCACTTTCAAAAGTCCCTGAACTTAAAAACGAAt                                                                                                                                                 >  2:686698/1‑151 (MQ=255)
        cAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATc                                                                                                                                                >  4:1213845/1‑151 (MQ=255)
        cAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCAAACGAATc                                                                                                                                                >  3:720115/1‑151 (MQ=255)
          aggaTTACATGAATATTTGAATTAGCGATGGGGGGGTTCGCGAGAGTCCTATGGGAGCTGAATGTAAATGTGCCGTGATGGTCTTAGATGGTACTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCg                                                                                                                                              <  4:815914/148‑1 (MQ=9)
            cATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAACAAGCGAATCCGc                                                                                                                                             >  4:1319386/1‑150 (MQ=255)
              cctttctaaaaTTTTAATTACCGCTGGTTGGGTTCCCGAGAGTCCTTAGCGACCTGAATCTAAAGGATCCGCCATGGTCTTAGATCTGTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCaa                                                                                                                                           <  4:1504639/143‑1 (MQ=2)
               tACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAACCAACGAATCCGCAAt                                                                                                                                          >  4:1171709/1‑150 (MQ=255)
                aCCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGTATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAGCAAGCGAATCCGCAAtc                                                                                                                                         >  2:544413/1‑149 (MQ=21)
                 gatCAATATTTGAATTACCCCTGGTCGGGTTCGCGAGAGTGCTTTGGGAGCAGACTGTAAATCTTCCGTGATGGTCTTAGATGTTAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAAtca                                                                                                                                        <  2:1183742/148‑3 (MQ=9)
                  cccAAACTTAGAATTAACGCTGGTGGGGTACCCGAGCGTACAAAGGGACCAGACTGGAAAGCAGCCGTGAGCTCCTTCGAACGGACGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTaaa                                                                                                                                      <  1:259573/149‑1 (MQ=18)
                  atCAAAATATGAATTACCCCTGGGTGGGTTCCCGAGAGCACAAAGGCAGAAGACTCTAAATCTGCCGTCATCCACTTCGAATGTTAGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTaaa                                                                                                                                      <  2:782703/150‑1 (MQ=21)
                   tCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCTTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTGGCCAGCGAATCCGCACTTagc                                                                                                                                      >  3:907565/1‑148 (MQ=21)
                   acAAAATTTTAATTACCGCTGGGGGGGTTCCCGAGCGTCCTTAGGGAGCAGACTGTTAATGTCCCGTGAGCGTCTTCGAATGTTAGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAat                                                                                                                                     <  6:403865/150‑1 (MQ=25)
                      ttaTTTGAATTTCGCCTTGGGGGGTTCCCTCGAGGCCTAAGTGTGCAGACTGTAACTATGCCGTCATCGTCTTCGATTGTTAGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAat                                                                                                                                     <  4:345817/146‑1 (MQ=21)
                      aactttGAATTACCCCTGGTGGGGTTCCCGAGCGCCCCAAGGGACCCGACTGTACATCTCCCGTCATGGTCTTAGATGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATAtt                                                                                                                                  <  1:1215860/148‑1 (MQ=18)
                        atatGAATTATCGCTGCGGGGGTACCCGAGCGGTCAATGGCAGACGAATGTAAATCAGGCGTCATCGACTACGCATCGAAGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCt                                                                                                                                <  1:617000/148‑1 (MQ=12)
                        aTTTTAATTACCGCTGGTGGGGTTCCCGAGCGGACTAAGGCAGCAGACTGTTAATCTGCCGTGATCGACTTCGACGGTTATAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCt                                                                                                                                <  8:805323/151‑1 (MQ=25)
                        aTTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTACCAAGCGAATCCGCaagacaagactac                                                                                                                                >  5:914544/1‑140 (MQ=18)
                          ttGATTTACCCCTGGTGGGGTTCCCTAGCTGCCAAAGGGAGCAGACTGTAACTCTGCCGTCATGGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTg                                                                                                                               <  5:745632/150‑1 (MQ=255)
                            gAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGccc                                                                                                                            >  7:1804277/1‑151 (MQ=255)
                            gAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTGACCACCGAATCCGCAATCAAATATTCTGccc                                                                                                                            >  6:1277975/1‑151 (MQ=255)
                             aaTTACCCATGGGGGGGTTCCCGAGCGGCCAAAGCGAGCAGAATGTCACTCAGCCGTGATCGCCTTCGACGGTTAGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCa                                                                                                                           <  5:1684398/151‑1 (MQ=25)
                              aTTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACGTTCAAAAGTCCCTGAACTTGCCACCGAATCCGCAATGAAATATTCTGCCCAt                                                                                                                          >  3:388531/1‑151 (MQ=255)
                                tACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAACAAACGAATCCGCAATAAAATATTCTGCCCATGc                                                                                                                        >  6:144786/1‑151 (MQ=255)
                                  ccccTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATAAAATATTCTGCCCATGCgg                                                                                                                      >  8:1373321/1‑151 (MQ=255)
                                  ccccTGGTGGGGTTCCCGAGCGCGCAAAGGGTGCAGAATGTAAATCTGCCGTCATCGCCTTCGATGGTTGGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCgg                                                                                                                      <  4:514722/151‑1 (MQ=18)
                                      tggtggGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATAAAATATTCTGCCCATGCGGGGa                                                                                                                   >  5:1473608/1‑150 (MQ=255)
                                       ggtggGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAACAAACGAATCCGCAATATAATAATATGCTCATGAAGGGaa                                                                                                                  >  8:948233/1‑150 (MQ=17)
                                       ggtggGGTTCCCGAGCGCCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTAGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGa                                                                                                                   <  7:1331947/149‑1 (MQ=255)
                                          ggggGTCCCGAGCGTCCAAAGGGCGCAGACTCTAACTCTGCCGGGATCCCCGGCGCCCGGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGAt                                                                                                              <  3:1417632/151‑1 (MQ=2)
                                          ggggCTCCCGAGCGGCCCACGGGAGCAGACTGTACCTCTGCCGGCATCGCCTGCGCCCGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGAt                                                                                                              <  5:1278483/151‑1 (MQ=14)
                                             gTTCCCGAGCGGCCAAAGCGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGGTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATgag                                                                                                           <  7:548691/151‑1 (MQ=255)
                                              ttCCCGAGCGGCAAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTACAAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATg                                                                                                             >  2:1039016/1‑148 (MQ=255)
                                                cccGAGAGGCCAAAGGGAGCAGACTTTAAATCTGCCTTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCATGAACTTCACAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGaa                                                                                                         <  4:1024887/150‑1 (MQ=255)
                                                 ccgagagcactttgtgAGCAGACTGTTACTGTCCCGTGATCGACTTAGATTGTTATAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGc                                                                                                       <  2:1592253/136‑1 (MQ=17)
                                                  gcagatctcAAAGCCAGCAGACAGTAAAACACCCGCCATCCACTCCGACCGTTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCt                                                                                                      <  5:1051403/143‑1 (MQ=11)
                                                   gAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCAAGCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGcat                                                                                                     >  7:948899/1‑149 (MQ=255)
                                                   gAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAACAACCGAATCCGCAATAAAATATTCTGCCCATGCgg                                                                                                                      >  6:1123518/1‑134 (MQ=21)
                                                    aGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTc                                                                                                    >  8:453359/1‑151 (MQ=255)
                                                     gCGGCCAAAGGGACCAGACTGTAAATCTGCCGTCATCGACTTCGAACGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCg                                                                                                   <  1:793332/151‑1 (MQ=255)
                                                       ggCCAACGGGAGCAGACGGTAAATCAGCCGTCATCGACTTCGAACCTTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGAc                                                                                                 <  1:50765/151‑1 (MQ=18)
                                                         ccAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGAcg                                                                                                >  3:1063083/1‑149 (MQ=255)
                                                          cAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCaa                                                                                              <  1:1410508/151‑1 (MQ=255)
                                                          cAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAACCAACGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCttgatcgg                                                                                               >  6:1050999/1‑144 (MQ=255)
                                                             aGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTACCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGt                                                                                           >  5:357135/1‑151 (MQ=255)
                                                              gggtgtaGACTGTAATTGTGCGGTCATCGAGTTCGAAGGTTGGTTTCCTTCCCCCACCACCATCTCTTTGAAATGTCCCTGATCTTCCCAACGAATCCGGAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGAGCAAGGtt                                                                                          <  8:320075/145‑1 (MQ=18)
                                                               ggAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTGGCAACCGAATCCGCAATGAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTc                                                                                         >  1:1105874/1‑151 (MQ=18)
                                                               ggACCAGACTCTTAATCAGCCGTGATCGACTTCGAATGTACTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTc                                                                                         <  7:107212/151‑1 (MQ=18)
                                                               ggACCAGACACTAAAGCTGCCGGCATGCACTGCGAAGTGTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTc                                                                                         <  7:1502533/151‑1 (MQ=11)
                                                                  cccgACTGTAAATCTCCCGTCATCCCCTTCGACTGTTAGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGAc                                                                                      <  2:1815772/148‑1 (MQ=18)
                                                                       atGTAAATCTGCGGTCATGTACTTCGAATGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAg                                                                                 <  6:787007/150‑1 (MQ=255)
                                                                        tGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCAATATCAAAAGACCCTGAACTTCCAAAc                                                                                                                                                     >  4:1428609/1‑82 (MQ=17)
                                                                        cgtcactcggcggTCAGCGACTTCGCAGGGTAGAATCCTTCCCCCAGCACCTTCACTTTCATAAGTCCCTTAACTTCCCAACTAATCCGCAATTAAATATTCTGCGGATGCGGGGAAGTATGAGAAGCTTCGACCAAGGTTCGACTCGAgc                                                                                <  1:712382/139‑1 (MQ=18)
                                                                         gTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAg                                                                                 <  7:239540/149‑1 (MQ=255)
                                                                          tAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCggagctcggac                                                                              >  1:1686146/1‑141 (MQ=255)
                                                                           aacccTGCCGTCATCGACTTCGAACCTTCTAATCCTTCCCTCACAACCATCACTTTCAAAAGTCCCTTAACTTCCCAACGAATACGCAATTAAATATTCTGCCCATGCTGGGAAGTATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGcc                                                                             <  6:502716/147‑1 (MQ=18)
                                                                            aaTCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGcc                                                                             >  3:118400/1‑150 (MQ=255)
                                                                                 gCCGTGATGGTCTTCGATGGTTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAATCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCga                                                                        <  4:76763/150‑1 (MQ=14)
                                                                                  ccGTCATCGCCTTCGCATGTTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCgaga                                                                      <  4:884780/151‑1 (MQ=255)
                                                                                    gcgtatctcTTCGATGGTACTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAACCGAATCCGCAATCAAATATTCTGCCCTTGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCgagaga                                                                    <  7:629216/143‑1 (MQ=14)
                                                                                    gTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTACCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTCGAGCGACAGCgcg                                                                       >  6:1802424/1‑146 (MQ=255)
                                                                                      gatcgtctgagacggTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGc                                                                  <  8:1805317/138‑1 (MQ=14)
                                                                                      gaTCGTCTTAGATGTTTCTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAATCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGagagag                                                                   <  8:1574153/149‑1 (MQ=14)
                                                                                      cATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTACCAAACGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAgcgatcggaagagagg                                                                  >  2:274403/1‑138 (MQ=14)
                                                                                       aTCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGGCCCTGCCCTTCCCACCGAATCGTCAAATAAATATTCGGCCCATGCGGGGAATGATGAGAAGCTTCGACCAAg                                                                                             >  1:1560294/1‑123 (MQ=18)
                                                                                                    gttataaTCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCGATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCaa                                                    <  2:1800109/146‑1 (MQ=255)
                                                                                                            ccTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCACCGAATCCGCAATTAAATATTCTGCCCATGCGGGTAAGTATGAGAAGCTTCGACCACGGTTCGACTCGCGCGCCAGCGATAGAGCGTTGCCGCAGGCAACGACCCg                                             <  5:465853/150‑1 (MQ=14)
                                                                                                            ccTTCCCCAACCACCATCACTTTCAACATACCCGAACCTTTTCATCTATTCAGATTTTAAATTTTGTCACTCTGCGGGGAAGGTTGAGAAGCTTCGACAAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGa                                            <  6:618594/151‑1 (MQ=2)
                                                                                                                 ccccACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGCCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGc                                       >  5:583964/1‑151 (MQ=255)
                                                                                                                   cccccaccaTCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGa                                     <  8:606074/148‑1 (MQ=255)
                                                                                                                   ccaccaccaTCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGa                                     <  2:1328660/151‑1 (MQ=255)
                                                                                                                     accaccaTCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGAct                                                                                                >  6:338842/1‑89 (MQ=255)
                                                                                                                      ccaccaTCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgc                                  <  3:126564/151‑1 (MQ=255)
                                                                                                                       caccaTCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGa                                                 >  3:1637759/1‑135 (MQ=255)
                                                                                                                       caccaTCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcg                                 >  4:1586050/1‑151 (MQ=255)
                                                                                                                        accaTCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGACTCGAGCGACAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcgc                                >  3:794017/1‑151 (MQ=255)
                                                                                                                        accaTCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGGCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcac                                >  1:1806805/1‑149 (MQ=255)
                                                                                                                          caTCACTTTCAAAAGTCCCTGAACTAACAAACGAATCCGCAATAAAATATTCTTCCCATGCGGGGAAGGATGAGAAGATTCGCCAAAGCTTCGACTCCAGCGACAGCTAGAGATAGTTGACGCAGGCAACGACCCGAAGGGCGAAgcgcgc                              >  8:247251/1‑151 (MQ=11)
                                                                                                                          caTCACTTTCAAAAGTCCCTGAACTAACAAACGAATCAGCAATTAAAAATTAAGACAATGCGAAGAAGGATGAGAAGATTCTACAAAGGTTCGACTCGAGAGACAGAGATAGAGAGTTGCCGCAGGCAACGACCCGAAGGGCGAAgcgcgc                              >  4:653582/1‑151 (MQ=11)
                                                                                                                            tCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGcacatcgcaacc                            >  1:1478047/1‑140 (MQ=14)
                                                                                                                             cACTTTCAAACCGCCGTGACCTGCCCACCGAATCCGAAATTAAATATTCTGCCCAAGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCa                             <  3:51418/149‑1 (MQ=11)
                                                                                                                                tttCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgcgcg                        >  4:1770884/1‑150 (MQ=255)
                                                                                                                                   cAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTg                       >  8:528484/1‑149 (MQ=255)
                                                                                                                                     aaaGTCCCTGAACTTCCCAACGAATCCGCATTTAAATATTCTGCCCATGCGGGGAAGTTTGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTa                   <  6:140754/151‑1 (MQ=255)
                                                                                                                                          cccTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAACCTTCGACCAAGCTTCGACTCGAGCGACAGCGAGAGAGCGTTCCCGCAGGCAACGACCCGAAGCGCGAAGCGCGCAGCCCTCACTAATcc               >  6:244026/1‑150 (MQ=14)
                                                                                                                                           ccTGAACTTCCCAACGAATCCGCAATTAAATATTATGACCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTAGACTAGAGCGCCAGCGAGAGAGCGTTGCAGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCtt             >  2:1705192/1‑151 (MQ=255)
                                                                                                                                            cTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAACGGAGAAGCGCGCAGCGCTGACGAATCCTTc            >  7:393275/1‑151 (MQ=255)
                                                                                                                                            cTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGACTCGAGCGACAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGAGATCAGAACAGAGTAATcaagc            >  6:928157/1‑147 (MQ=14)
                                                                                                                                            cTGAACTTCCCAAAGAATCCGCAATTAAATATTCTGACAATGCGGGGAAGGATGAGAAGCTTCGACCAAGCTTCGACTCGAGCGACAGCGAGAGAGCGTTGACTCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTc            >  6:276903/1‑151 (MQ=14)
                                                                                                                                              gAACTTCCCAACGAATCCGCAATTAAATATTCTGCACATGAAGCGAAGCATTATGAGCTTCCCACAAGGTGAGAATAGACCGAAACCGAGAGAGCGTTGCAGAAGGCAACGCCCCGAAGGGCGGAGCGCGCAGCGGTGCGTAGTCGGTccc          >  3:1781162/1‑151 (MQ=255)
                                                                                                                                                 cTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAg                     >  6:1629246/1‑137 (MQ=255)
                                                                                                                                                      cAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATTAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCccaccacc  <  8:1201434/151‑1 (MQ=37)
                                                                                                                                                      cAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAg                                                                                                        >  1:675515/1‑49 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CACCATCTCAAGCATTACCTCAAAATTTGAATTACCCCTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTTCCCAACGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGCCGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACC  >  DH1/2591111‑2591411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑