Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,184,5850CA28.0% 281.3 / 47.3 143intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
Reads supporting (aligned to +/- strand):  ref base C (59/44);  new base A (37/3);  total (96/47)
Fisher's exact test for biased strand distribution p-value = 2.44e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

GAATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184436‑3184734
                                                                                                                                                     |                                                                                                                                                     
gAATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGTATCTGCTTCCCCCCTTGCCACTGACGACTCCTAGTCCCCCAGCCAATTTATTc                                                                                                                                                       >  1:436782/1‑150 (MQ=255)
 aaTCCCGTCGTAGCCTCCAACTTCTGAATGTATCGTATATGTTCTTCATATTCAAACCCATTTTTTTGGTGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCaa                                                                                                                                                     <  4:615379/151‑1 (MQ=21)
       ggcgtAGCCACCAAATTCTGAATGTATCGATTTTTTTCTTCTAATTGTATACCAATTTGTTGTTGTATCGCCTCGCCGTTATTTACCTGATTGTGTTTCCTGCTTTCCGTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCt                                                                                                                                               <  6:704849/149‑1 (MQ=255)
         cgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGaaggttt                                                                                                                                             >  8:1333279/1‑145 (MQ=255)
         cgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAGAGGcgtt                                                                                                                                             >  1:95126/1‑148 (MQ=255)
           tAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTATTAAAGATGCTTAcg                                                                                                                                           >  5:625518/1‑150 (MQ=255)
           tAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATAAAgcaccatacc                                                                                                                                           >  2:669727/1‑142 (MQ=21)
                   aaaTTCTGAATGTATCGAATATGTTCTGCAAATTGAAAACCATTTTGTTGGGGTATCGCCATGCGTTAATGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGt                                                                                                                                   <  7:1722120/151‑1 (MQ=25)
                         tGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGAAGCATACCTAGTAAGTGAAcc                                                                                                                              >  4:917880/1‑150 (MQ=21)
                           aaTGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGCAGGTTACGTTGTAAGTGCTccg                                                                                                                             >  3:596367/1‑148 (MQ=21)
                              gTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGtt                                                                                                                         <  8:485117/150‑1 (MQ=255)
                                aTCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAACCTTACCTAGTGAGTGCACCCAGTTggg                                                                                                                      >  3:1212237/1‑151 (MQ=255)
                                   gAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAAAAAAGAAGGTTACCTTGTAAGTGCTccttgtgtgggt                                                                                                                    >  7:1007988/1‑140 (MQ=18)
                                   gAATATGTTCGGAAAATTCAAAACCACGTTGTTGGGGTATCGCCAAGCGCCACGGCAGCGGAGGCTGACTCCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTa                                                                                                                   <  3:1097401/151‑1 (MQ=25)
                                     atatGTTCTGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATc                                                                                                                 <  6:446041/151‑1 (MQ=255)
                                      tatGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAATAAAGAAGCGTACCTGGTTAGTTCAGCGAGTTTGGGt                                                                                                                    >  3:1498507/1‑147 (MQ=18)
                                       atGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCg                                                                                                                <  6:141321/150‑1 (MQ=255)
                                         gTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAGAACCATACGTTGTAAGTTAACCCAGTAGGGGTATCTcc                                                                                                              >  3:234902/1‑150 (MQ=18)
                                           tCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTAGGCGTATCGCCa                                                                                                             >  8:407526/1‑149 (MQ=255)
                                            cGGCAAATTCAAAACCAATTTGTTGGGGGATCGCCAAGCTGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGc                                                                                                          <  3:1328063/151‑1 (MQ=255)
                                             ggCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCg                                                                                                         <  3:804639/151‑1 (MQ=255)
                                               cAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGCCGCTTACCTTGTAAGTGCAGCGAGTTGGGGTATCGCCAAGCGGt                                                                                                       >  7:1325239/1‑151 (MQ=255)
                                                aaaTTCATAACCAATTTGTGGGGGTATCGCCAAGCTGTAAGGCACCGGATTCTGACTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTa                                                                                                      <  2:813446/151‑1 (MQ=255)
                                                    tCAATACCATTTTGTTGGTGTATCGGATATCTTTAATGAACCGGATTGTGATTCAGGCTTTCCGAGGTTCGAATCCTCGTACCCCAGACTATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAgg                                                                                                   <  8:464753/150‑1 (MQ=25)
                                                     cAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGCTTACCTAGAAAGTGAACCGAGTGTGCGTATCTCCAAGCTGTAAGGc                                                                                                  >  8:1021563/1‑150 (MQ=18)
                                                     aaaaaTCAATTTCTTGTGTTATCGCCAAGCTGTAAGTCACGTGATTCTGTTTCAGGCTTTCATAGCTTCGAATCCTCGTACCCCAGAAACTATATTCAAGTCTCGTAACTTTTAACAGCAACTAGTTGGGGTATCGCCAAGCGGTAAGGCa                                                                                                 <  6:533438/150‑1 (MQ=255)
                                                      aaaaCCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGCATACCTTGTAAGTGAACCGAGTTTGCGTATCTACAAGCTCTAATGCAc                                                                                                >  8:1631648/1‑151 (MQ=18)
                                                              gttattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATc                                                                                                                 >  1:378090/5‑126 (MQ=255)
                                                              gttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATc                                                                                                                 <  2:379566/122‑1 (MQ=255)
                                                               ttgtggGGGTCTCGCCACGCCTGAATGCACCTGATTCTGTTTCCGGCAGTCCGCGGTTCGAATCCTCGTACCGCAGCCACTTTATTAAAGCCGCTTACATTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCt                                                                                        <  1:1784386/150‑1 (MQ=25)
                                                                tgtttGGGTATCTGCCACCGGTAATGATCATGATTCTGTTTCCGGCTCTCCGAGGTTCGAATCCTCGTACGCCAGCAATTTTATTCTAGTCGCTTAACTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTa                                                                                       <  2:550948/150‑1 (MQ=25)
                                                                tattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTGTGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCATGTAAGTGCACCCAGtt                                                                                                                         >  6:716380/3‑116 (MQ=21)
                                                                tattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGtt                                                                                                                         <  5:716629/114‑1 (MQ=255)
                                                                   tgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCGTGGTTCGAATCCTCGTACCCCAGCCAAATTATTCAAGACGc                                                                                                                                                >  5:601281/1‑90 (MQ=14)
                                                                   tgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGc                                                                                                                                                <  6:601275/90‑1 (MQ=255)
                                                                      gtttttccctaccGGTACGGCTTCGGATGGTGATTCCGGCTTTCCGAGGTTCGAATCCTCGTACCCCAGACAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTc                                                                                   <  8:1585576/136‑1 (MQ=37)
                                                                        tATCGCCAAGCGGTAAGGCACCGGATTTTGATTCCGGCATTCCGTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCAccc                                                                                                                             <  6:260342/104‑1 (MQ=255)
                                                                        tATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGCTTACCTAGTAAGTGAACCGAGTTGGGGTATCGCCAAGCGGTAAGGCACGGGAGTCTACTTCCGGc                                                                               >  7:75301/1‑150 (MQ=25)
                                                                            gCCAAGCGGTAAGGCACCGGTTTGTGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCAccc                                                                                                                             >  4:1261175/1‑100 (MQ=21)
                                                                            gCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTg                                                                                                                                        <  1:590816/89‑1 (MQ=255)
                                                                            gCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCAccc                                                                                                                             <  3:1258099/100‑1 (MQ=255)
                                                                                  cGGTAAGGCACCGGTTTTTGATACCGGCATTCCGTGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGagggtt                                                                                                                                              >  1:1056854/1‑72 (MQ=14)
                                                                                  cGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCtt                                                                                                                                              <  2:1059983/77‑1 (MQ=255)
                                                                                   ggtactgCACCGGATGGTGACTCAGGGTTTCCTAGGTTCGAATCCTCGTACCCCAGCCAATTTCTTATAGACGCTTCAATTGTAAGTGCACGCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTc                                                                   <  1:1291732/145‑1 (MQ=21)
                                                                                   ggTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTAc                                                                                                                                            >  7:465504/1‑78 (MQ=14)
                                                                                    gTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAATAAAGACCCTTTCGTTGTTATTTAACCCATTTTGGGTAGCGCCAATGGGTAAGGCAACGGATTCAACTTCGGGCATTcagtgtttgg                                                                  >  7:1750854/1‑142 (MQ=12)
                                                                                       aGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGACGCTTACCTTGTAAGTTAACCGAGTGGGGGTAGAGCCAAGCGGTAAGGCACAGGATTCTAATTGAGGCATTCAGAGTTTCGaa                                                                >  7:1238334/1‑150 (MQ=25)
                                                                                        ggCACCGGATTCTGAGTCAGGCACTCCGAGGGTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATc                                                              <  7:699775/151‑1 (MQ=255)
                                                                                         gcaaatgattgtgtctCCGGCATTACGACGTTCGAATCCTCGTACCGCTGAATCTATATACAAGTGTCTTAAATTGAAATAGCACTCTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATcc                                                             <  3:797237/136‑1 (MQ=9)
                                                                                            ccggcggcggacaccGGCACTCCGCGGGGCGAAGCCTCGTACCCCAGCCTTTTTATTCAAGCCGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCg                                                          <  1:503035/138‑1 (MQ=21)
                                                                                             cGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAACAAGAACCATACGGAGAAAGTTATCCAAGTTGGGGTAAAGGCAATCTCTAATGCACAGGATTCTAATACATGCATTCCGAgtagccactcctcct                                                         >  7:59196/1‑137 (MQ=2)
                                                                                                  tCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAGAGCCTTACCTGGTAAGTGATCCGATTTTGGGTAGAGTCAAGCGGTAAGGCGCGGTAGTCTAATTCGGTCATTCAGAGTCTCGAAACATCGTCcccc                                                    >  7:130097/1‑151 (MQ=12)
                                                                                                      aTTCCGGCACTCCTAGCGTCGAAGCCACGTAACCCAGCCAATTTATTCAAGCCTCTTACCTTGGAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGcc                                                <  7:706836/151‑1 (MQ=21)
                                                                                                        tCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGCTTACCTGGTAAGTGGACCGAGTTGGGGTATCGGCAAGCGGTAAGGCGCCGGAGTCTAATTCCGGCATTCCGAGTTTCGAATCCTCGTCCCCCCGCcac                                              >  1:1467031/1‑151 (MQ=38)
                                                                                                                tCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGCGTACCTGGTAAGTGCACCCAGTTGGGGTATCGACAAGCGGTAAGGCACGGGAGTCTACTTCAGGCATTCAGAGGTTCGTGTCAGCCTACCCCCGCCACATTaaaa                                       >  5:753971/1‑150 (MQ=255)
                                                                                                                 ccGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTa                                                                                                                                             >  4:690761/1‑47 (MQ=255)
                                                                                                                 ccGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTa                                                                                                                                             <  3:688401/47‑1 (MQ=255)
                                                                                                                 ccGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAGGCTTACCTTGTAAGTGAACCCAGTTGGGGTATAGACAAGCGGTAATGCACAGGATTCTAATTACGGCATTCCGAGTATCGAATCCTCGTCCCCCAGCCACATTaaaaa                                      >  5:96986/1‑150 (MQ=255)
                                                                                                                   gAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAACCATACCTTGTAAGTTCACCAAGTTGGGGTATCGACAATCTCTAAGGCAAAGGATACTAATTAAATCATTCAGAGTATCGAATCATCCTACCCCAGCAAAATTaaaaaacc                                   >  4:886133/1‑149 (MQ=255)
                                                                                                                    cggatCGAAGCCACCTACCCCAGCCAATATATTCAATACGCTTACCTTTTAATTGCACCGAGTTGGGGTATCGCCAATCTTTAAGGCACCTGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCt                                  <  7:1223742/147‑1 (MQ=21)
                                                                                                                     ggTTCGAATCCTCGTACCCCAGCCAATTTAATAAAGAGGCCTACCTAGTACGTGCACCGATTAGGGGTTTAGACAATCTGTAAGCCACAGGATTCAAATGCCGTCATTCAGAGTATGCACTCCTCCTACCCCAGCGAAATAaaaaaaccg                                  >  5:1000219/1‑147 (MQ=255)
                                                                                                                         cGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGACTCCTCGTCCCCCCGCCACATTAAAAAAGCTCGCtt                             >  3:1477443/1‑151 (MQ=255)
                                                                                                                         cGAATCCTCGTACCCCAGCCAATTTATTAAAGACGCTTACCTTGTAAGTGCACCCAGTTTGGGTATCGACAAGCGCTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGca                              >  3:1676225/1‑149 (MQ=255)
                                                                                                                         cGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGCTTACCTTGTAAGTGAACCCAGTTGGGGTATCGACAAGCGGTAAGGCACCGGATTCTAATTCAGGCATTCCGAGTATCGAATCATCCTACCCCAGCCACATTAAAAAACCTCGCtt                             >  4:1690456/1‑151 (MQ=25)
                                                                                                                          gAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACAGGATTCTAATACCGGCATTCCCAGGATCGAATCCTCGTACCCCAGCCACATTAAAAAagctagctac                            >  6:1531791/1‑149 (MQ=37)
                                                                                                                          gAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATAGCCAAGCGGTAAGGCACCGGATTCTAATTCAGGCATTCCGAGGATCGAAACCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTc                            >  2:830404/1‑151 (MQ=255)
                                                                                                                          gAATCCTCGTACCCCAGCCAATTTATTAAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTc                            >  4:495831/1‑151 (MQ=255)
                                                                                                                          gAATACTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTACGGGTATCGCCAACCGGTAAGGCACCGGAATCTAATTCCGGCATTCCGACGATCGACTCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTc                            >  4:918061/1‑151 (MQ=255)
                                                                                                                           aaTCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCg                           >  5:316535/1‑151 (MQ=255)
                                                                                                                           aaTCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCg                           >  5:314298/1‑151 (MQ=255)
                                                                                                                           aaTCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATT‑AAAAAGCTCGCTTCgg                          >  7:119523/1‑151 (MQ=255)
                                                                                                                            aTCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCgg                          >  7:1469485/1‑151 (MQ=255)
                                                                                                                               cTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  7:269056/1‑150 (MQ=255)
                                                                                                                               cTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  1:1792446/1‑150 (MQ=255)
                                                                                                                               cTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  5:104191/1‑150 (MQ=255)
                                                                                                                                 cGTACTTCAGACAATTTAGTCAAGACGCTTCATTTGTAACTGCAACTAGTTGGGGTAGGGCGACGCTGGAAGGCACCGGATTCTCATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGa                       <  7:980965/149‑1 (MQ=18)
                                                                                                                                    aCCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttt                  >  7:253487/1‑151 (MQ=255)
                                                                                                                                    aCCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttt                  >  6:1373576/1‑151 (MQ=255)
                                                                                                                                    aCCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAAGTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtgt                  >  2:309422/1‑149 (MQ=255)
                                                                                                                                     ccccAGCCACTATCTTCAAGGCTCTTAACTTGCAACAGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtttt                 <  7:1793114/151‑1 (MQ=21)
                                                                                                                                      cccAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttttt                >  4:899610/1‑151 (MQ=255)
                                                                                                                                       ccAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATACTCGTACCCCAGCCACATTAAAAAAGCTAGCTTCGGCGAGCTTTTTg               >  1:452726/1‑151 (MQ=255)
                                                                                                                                        cAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTgg              >  7:1495853/1‑150 (MQ=255)
                                                                                                                                        cAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGc              >  6:980293/1‑151 (MQ=255)
                                                                                                                                         aGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCt             >  7:22849/1‑151 (MQ=255)
                                                                                                                                          gCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtt            >  3:1651032/1‑151 (MQ=255)
                                                                                                                                          gCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtt            >  5:211817/1‑151 (MQ=255)
                                                                                                                                           ccAATTTATTCAAGCCGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCttt           >  5:612051/1‑151 (MQ=255)
                                                                                                                                            cAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          >  5:1761696/1‑151 (MQ=255)
                                                                                                                                             aaTTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTc         >  3:1525434/1‑151 (MQ=255)
                                                                                                                                             aaTTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTc         >  3:1817502/1‑151 (MQ=255)
                                                                                                                                              aTTTATTCAAGCCGCTTACCTTGTAAGTCCACCCAGTTGGGGTATCGCCAACCGGTAAGCCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCt        >  5:927228/1‑151 (MQ=255)
                                                                                                                                              aTTTATTCAAGCCGCTGACCTTGCAAGAGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCt        <  7:1332063/151‑1 (MQ=255)
                                                                                                                                              aTTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCt        >  7:1123434/1‑151 (MQ=255)
                                                                                                                                              aTTTATACAATATGCTTCCATTTAAATTGCAATCTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCt        <  8:1084711/151‑1 (MQ=18)
                                                                                                                                                  aTTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGt    >  8:52871/1‑151 (MQ=255)
                                                                                                                                                  aTTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGt    >  1:614115/1‑151 (MQ=255)
                                                                                                                                                   ttCAAGACGCTTACCTTGTAAGTGCACCCAGATGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTCCGtt   >  2:610373/1‑151 (MQ=255)
                                                                                                                                                     cAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  6:1134883/1‑150 (MQ=255)
                                                                                                                                                     cAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  6:495215/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184436‑3184734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑