Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,447,4030AC24.2% 684.1 / 90.7 368G346G (GGT→GGG) malZalpha‑glycosidase
Reads supporting (aligned to +/- strand):  ref base A (77/201);  new base C (88/1);  total (165/203)
Fisher's exact test for biased strand distribution p-value = 4.29e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 378 total aligned reads displayed.

CCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGC  >  DH1/3447254‑3447552
                                                                                                                                                     |                                                                                                                                                      
 cACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCcccccc                                                                                                                                                      >  3:1349664/1‑151 (MQ=255)
 cACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCAcc                                                                                                                                                      >  3:308012/1‑151 (MQ=255)
          tCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTccc                                                                                                                                              <  7:542508/150‑1 (MQ=255)
           cATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTccccc                                                                                                                                            <  2:496340/151‑1 (MQ=255)
            aTGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCa                                                                                                                                           <  7:1314546/151‑1 (MQ=255)
            aTGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGGTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCa                                                                                                                                           <  1:585042/151‑1 (MQ=255)
               gcggcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCCGc                                                                                                                                         >  3:589785/1‑150 (MQ=255)
               gcggcATCTTCCACACCGGCCTGTAACCATTGCCGTGCATCGCCAAAATCTTCGCCGACAAAATACGCTTCCGGCTGGGGCGCTTTCGCCGCCTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCa                                                                                                                                        <  6:1204565/151‑1 (MQ=255)
                  gcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCatat                                                                                                                                     >  1:1649455/1‑151 (MQ=255)
                   cATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATg                                                                                                                                    <  3:885122/151‑1 (MQ=255)
                    aTCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCACCATATGc                                                                                                                                   >  1:633260/1‑151 (MQ=255)
                    aTCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGCCCCCAGCATATGc                                                                                                                                   >  7:853319/1‑151 (MQ=255)
                     tCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGcc                                                                                                                                  >  2:1100034/1‑150 (MQ=255)
                       ttCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTCCATATTATTGCGCGCCCCCCCCGCCACCCCCAGCATATGcacc                                                                                                                                >  2:1349904/1‑151 (MQ=255)
                         ccACATCGGCCGGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGcacca                                                                                                                               <  6:1407930/150‑1 (MQ=255)
                          cacaTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACcaca                                                                                                                             <  5:1109889/151‑1 (MQ=255)
                               cGGCCTGTATCCTTTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATcca                                                                                                                         <  2:1618090/150‑1 (MQ=255)
                               cGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCACATccac                                                                                                                        >  1:446137/1‑150 (MQ=255)
                                ggCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCACATccagc                                                                                                                       >  7:730561/1‑151 (MQ=255)
                                  ccTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCTCCCCCAGCATATGCACCCCATcccgccc                                                                                                                     >  3:228513/1‑150 (MQ=255)
                                  ccTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccg                                                                                                                     <  5:221663/151‑1 (MQ=255)
                                  ccTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCCGCCCCCCCCATATGGCCCACATccagccc                                                                                                                     >  1:19469/1‑150 (MQ=255)
                                   cTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCAGCATATGCACCACATccacccgc                                                                                                                    >  4:1028371/1‑151 (MQ=255)
                                       aaCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccgccag                                                                                                                 <  8:621393/150‑1 (MQ=255)
                                        aCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACCTcccgccgccagcc                                                                                                               >  5:1550985/1‑151 (MQ=255)
                                        aCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATccagccgccagcc                                                                                                               <  5:95181/151‑1 (MQ=255)
                                          cATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCAGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGt                                                                                                             <  8:1238156/151‑1 (MQ=255)
                                            ttGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCCGCCTATGCCCCCCATcccgcccgcagccctgc                                                                                                           >  6:224196/1‑147 (MQ=255)
                                              gCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCat                                                                                                         <  5:1808103/151‑1 (MQ=255)
                                               ccGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCata                                                                                                        <  7:482394/151‑1 (MQ=255)
                                                cGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGGTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCatat                                                                                                       <  7:1087448/151‑1 (MQ=255)
                                                  tGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTAGGGTTCTTTCGCCGCTTCGGTGATCCCGGAAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCatat                                                                                                       <  1:1037292/149‑1 (MQ=255)
                                                    cATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCa                                                                                                   <  3:459191/151‑1 (MQ=255)
                                                       cGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCCCCAGATGCCCCACATCCACCCGCCAGCCGGCCCAATTCCCCgg                                                                                                >  5:1304106/1‑151 (MQ=255)
                                                           aaaaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCCCCCAATGCCCCCCCTCCCGCCCCCAGCCCTGCCTATTACAAGGCGCt                                                                                            >  4:899074/1‑151 (MQ=255)
                                                            aaaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCCCCCTATGGCCCCCCGCCACCCGCCCGCCGTCCCTATTACACGgcgcgt                                                                                           >  5:384945/1‑149 (MQ=255)
                                                                ggtCGCCGACAATATACGCTTCCGGCGGGGGGTCTTTCGCCGCTGCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGGCCATATTCCACGGCGCTTTCa                                                                                        <  4:707230/148‑1 (MQ=255)
                                                                gTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAg                                                                                       <  7:1470162/151‑1 (MQ=255)
                                                                gTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAg                                                                                       <  7:941287/151‑1 (MQ=255)
                                                                gTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAg                                                                                       <  4:1487178/151‑1 (MQ=255)
                                                                  tCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGc                                                                                      <  1:1461604/150‑1 (MQ=255)
                                                                   cGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCAGCATATGCACCACATCCAGCCGCCCGCCGTCCATATTACACGGCGCTTTCAGccc                                                                                    >  3:52664/1‑150 (MQ=255)
                                                                   cGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCa                                                                                    <  6:111840/151‑1 (MQ=255)
                                                                     ccGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCTCTTCGGTGATCCAGGCAACGTGCTGCATATTATTGCGCGCCCCCACCGCCGCCCCCCCCAAATTCCCCACATCCCACCCCCAACCGTCCCTAATACACGGCGCATTAAGcccc                                                                                   >  8:876496/1‑148 (MQ=255)
                                                                        aCAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTGCCCCCGCATATGGACCCCATGCAGCCGCCCGGCGGGCATATTGCACGGCGCGTTGAGCCCGttgg                                                                               >  8:358434/1‑148 (MQ=255)
                                                                              taCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGCCCCCAGCATATGCACCCCATCCAGCCGCCAGCCCTCCATATTCCCCGGCGCTTTCAGCCACTGGCGGAcc                                                                          >  5:1867081/1‑149 (MQ=255)
                                                                                 gCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCAGCATATGCACCACATACAGCCGCCCGCCGGCCATATTCCACGGCGCGTTCAGCCCGTGGCGGCCAATa                                                                       >  3:1757561/1‑150 (MQ=255)
                                                                                 gCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATAc                                                                      <  8:1547340/151‑1 (MQ=255)
                                                                                 gCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCCGCCCCCACCCGATGCCCCACCTCCCGCCCCCCGCCGGGCCTATTGCCCGGCGCGTTCCCGCCGTGGCggcccaaac                                                                      >  4:552241/1‑144 (MQ=255)
                                                                                   ttCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTg                                                                    >  3:1012468/1‑151 (MQ=255)
                                                                                     ccGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGt                                                                   <  2:1565769/150‑1 (MQ=255)
                                                                                     ccGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTc                                                                  <  7:636311/151‑1 (MQ=255)
                                                                                       ggCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCtt                                                                <  1:963531/151‑1 (MQ=255)
                                                                                        gCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑c                                                              <  2:1825754/151‑1 (MQ=255)
                                                                                        gCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCACCCCCCGCAAATGCACCACATCCAGCCGCCAGCCGTCCAAATTACACGGCGCTTTGAGCCAAAGGCGGACAATAcaggagtc                                                               >  2:808743/1‑144 (MQ=255)
                                                                                           gggTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑cccc                                                           <  2:742157/151‑1 (MQ=255)
                                                                                               ttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCcgcg                                                        <  8:53419/150‑1 (MQ=255)
                                                                                                tctttcGCCGCTTCGGTGATCCGGGCAAGGTGCTGCATATTATTGCGCGCCGCACCCGCCTCGCGCAGCATATGCACCACATCCAGCCGCCAGCCGTCCTTATTCCACGGCGCTTTCAGCCAGTGGCGGAGAATACTGTCTT‑CCCCGCGa                                                       <  2:320337/150‑1 (MQ=255)
                                                                                                  tttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCACCCTATTGACCACATGCACCCGGCACCCCGCCCCAATCCACCGCGGATTTAGCCCGTGGCCGCCCATAATTGCTT‑GCCcgcgcgt                                                     >  7:1575800/1‑147 (MQ=255)
                                                                                                   ttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATaa                                                    <  6:933807/150‑1 (MQ=255)
                                                                                                   atctccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGAt                                                      <  6:1840580/144‑1 (MQ=255)
                                                                                                    tcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATaaa                                                   <  4:1818759/150‑1 (MQ=255)
                                                                                                     cgccgcTTCGGTGATCGCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCTGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAAt                                                  <  5:1450393/150‑1 (MQ=255)
                                                                                                      gccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAAttt                                                <  5:1744057/151‑1 (MQ=255)
                                                                                                       gcgcTTCGGTGATCCCGGCAACGTGGTGCATATTATTGCGCGCCCCACGCGCCTCCCCCAGCATATGCACGACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATttc                                               <  6:521394/150‑1 (MQ=255)
                                                                                                       ccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATttc                                               <  1:1100533/151‑1 (MQ=255)
                                                                                                           ttCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGCCCCCCCCATATGGACCCCCTCCACCCCCCCCCCCTGCAAATTAAACCGCCCGTTGAGCCCGTGGCGGGCAATAATGTGTT‑CCCCGCGCTAAAAttgatt                                            >  1:290466/1‑150 (MQ=255)
                                                                                                           gtCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATttcattc                                           <  5:815275/150‑1 (MQ=255)
                                                                                                            tCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCCCCAAATTCCCCCCATCCAGCCGCCCGCCGTCCCTATTGCCCGGCGCTTTCAGCCCGTTGCGGCCAATACGGTCTT‑GCCCGCGATAAATttccttg                                           >  7:316433/1‑149 (MQ=255)
                                                                                                            tCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATttcattc                                           <  1:1780757/150‑1 (MQ=255)
                                                                                                            tCGGTGATACCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTCTCTT‑CCCCGCGATAAATttgatt                                            <  4:82031/149‑1 (MQ=255)
                                                                                                            gcGGTGATCCCGGCAACGTTCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCATCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATttcatt                                            <  5:668078/148‑1 (MQ=255)
                                                                                                               gTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCCGCATATGCCCCCCCGCCCCCCCCCCGCCGGCCCAATTACACGGCGCCTTGAGCCCGGGGCGGCCCATACGGGGTTGCCCCGCCTTAAA‑TTCATTGAccc                                       >  1:342340/1‑150 (MQ=255)
                                                                                                                tGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGCCAATACGGTCGT‑CCCCGCGATAAATTTCATTCAcc                                        >  2:1044862/1‑149 (MQ=255)
                                                                                                                tGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCAGCAAATGCACCACAACCAGCCGCCCGCCGTCCAGAATACCCCGCCCGTTACGCCAGTGGCGGCCAAAACAGTCTT‑CCCCGCGATAAATTTCATTCAcc                                        >  6:335276/1‑149 (MQ=255)
                                                                                                                 gATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCCGCCGTCCATATTACCCGGCGCTTTGAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAgc                                     >  3:1275523/1‑150 (MQ=255)
                                                                                                                 gATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAg                                      <  8:1135654/150‑1 (MQ=255)
                                                                                                                 gATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGa                                     <  6:735873/151‑1 (MQ=255)
                                                                                                                 gATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGa                                     <  7:525900/151‑1 (MQ=255)
                                                                                                                    cccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACtt                                  <  3:1192790/151‑1 (MQ=255)
                                                                                                                    cccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACt                                   <  4:1354536/150‑1 (MQ=255)
                                                                                                                        gCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTAACCAGACTTTccc                              >  4:426697/1‑150 (MQ=255)
                                                                                                                         cAACGTGCTGCATATTATTGCCCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGa                             <  3:820297/151‑1 (MQ=255)
                                                                                                                          aaCGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCCGCCTATGCCCCACATCCCGCCGCCCGCCGTCCATATTCCCCGGCGCTTTCAGCCCGTGGCGGCCAAAACGGTCTT‑GCCCGCGCTAAAATTAATTGACCAGACGTTACGAc                            >  7:1764183/1‑151 (MQ=255)
                                                                                                                          aaCGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCCCCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGCCAATACTGTCGT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGAc                            >  3:1664881/1‑151 (MQ=255)
                                                                                                                           aCGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGGCCATATTCCACGGCGCGTTCAGCCAGGGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACt                           <  6:1644247/151‑1 (MQ=255)
                                                                                                                           aCGTGCTGCATATTATTGCGCGCCCCACCCGCCTACCCCAGCATATGCACCACAACCAGCCCCCACCCGTCCATATTACACGGCGCTTTCCTCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTAATTAACAAGCCGTTCCGAcg                           >  6:1082339/1‑150 (MQ=255)
                                                                                                                            cGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACTg                          <  5:889337/151‑1 (MQ=255)
                                                                                                                                     tattatTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATcc                   >  2:649687/1‑149 (MQ=255)
                                                                                                                                      attattGCGCGCCCCACCCGCCTCCCCCAGCTTATGCACCACATCCAGCGGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACATTACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGc                <  8:213278/151‑1 (MQ=255)
                                                                                                                                          ttGCGCGCCCCCCCCGCCTCCCCCCGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCg            >  6:784047/1‑151 (MQ=255)
                                                                                                                                          ttGCGCGCCCCCCCCGCCTCCCCCAGCATATGCACCCCATCCAGCCGCCAGCCGTCCATATTACACGGCGCTTTAAGCCAGTGGCGGACAAAACTGTATT‑CCCCGCGATTAATTTAATTCACCAGCCTTTCCGACCGATAATCCAGCTTCg            >  5:285641/1‑151 (MQ=255)
                                                                                                                                          ttGCGCGCCCCACCCGCCTCCCCCAGCAAATGCACCACATCCAGCCGCCAGCCCTCCATATTACACGGCGCGTTCAGCCAGTGGCGGACAATACTGTCTT‑ACCCGCGATAAATTTAATTCACCAGACTTTCCGACTGATAATCCACCTTCg            >  2:1794446/1‑151 (MQ=255)
                                                                                                                                             cgcgcCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCa         <  6:436181/151‑1 (MQ=255)
                                                                                                                                             cgcgcCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCa         <  1:468393/151‑1 (MQ=255)
                                                                                                                                             cgcgcCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCa         <  4:1423617/151‑1 (MQ=255)
                                                                                                                                               cgcCCCACCCGCCTCCCCCAGCATATGCACCTCATCCAGCCGCCAGCCGTCCATATTCCACTGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCATACTTTCCGACTGATAATCCAGCTTCGGCAAg       <  8:1514263/151‑1 (MQ=255)
                                                                                                                                                gcCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGc      <  4:1230592/151‑1 (MQ=255)
                                                                                                                                                    cACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGc  <  8:1374375/151‑1 (MQ=255)
                                                                                                                                                     aCCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACCGATAATCCAGCTTCGGCAAGCTGGc  >  8:1762913/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATATGCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTT‑CCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGC  >  DH1/3447254‑3447552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑