Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,448,5260AC30.2% 618.1 / 106.6 370intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
Reads supporting (aligned to +/- strand):  ref base A (200/54);  new base C (2/108);  total (202/168)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 389 total aligned reads displayed.

AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAAC  >  DH1/3448376‑3448675
                                                                                                                                                      |                                                                                                                                                     
 gCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGAc                                                                                                                                                      >  5:1044642/1‑151 (MQ=255)
  cAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACg                                                                                                                                                     <  2:304525/151‑1 (MQ=255)
   aGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgg                                                                                                                                                    <  7:415647/151‑1 (MQ=255)
        aTCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTTATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGc                                                                                                                                                <  5:1024502/150‑1 (MQ=255)
        aTCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGTCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGCACGGGGGc                                                                                                                                                <  4:587443/150‑1 (MQ=255)
            ttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCTTGCATTTAACATCATAATTCCCGTGTTATTAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATATTTGGGGGAGGCGGACGGGGGCgg                                                                                                                                              <  6:341070/148‑1 (MQ=255)
            ttgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGAt                                                                                                                                            >  5:80901/1‑150 (MQ=255)
                tttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACtt                                                                                                                                       >  8:1457327/1‑151 (MQ=255)
                  tgattAACAACTGGGGGCACCGGCAGGTGACATGCATTTAACAACATAATTCCCCTGTTATCAAAATCTGATCCCCTTGCCATACCCTAATGCAGGATCACTCATCATCTCAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACttt                                                                                                                                      <  8:525196/147‑1 (MQ=255)
                  tgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGTTAACtttg                                                                                                                                     >  4:441195/1‑150 (MQ=255)
                  tgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTc                                                                                                                                     >  6:1570685/1‑151 (MQ=255)
                  tctatAACAAAGGGGGGCACCGGCAGGTGCCATGAATTTAAAATCAAAATTCCCGTGTGATGAAAATCTTCCCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATTTTTGGGGGCGGCGGCCGGGGGCGGATAACttt                                                                                                                                      <  8:1830572/146‑1 (MQ=255)
                     ttAACTACTGGGGGCCGCGGGTGGGGCCATGCATTTAACTTCATATTTCCCCTGTTATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGACGGATTTTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCgg                                                                                                                                   <  1:1404174/150‑1 (MQ=255)
                      tAACACTTGGGGGCACCGGCATTTGCGATGTATTTAACATCTTAATTCCCGTTTTATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGa                                                                                                                                  <  3:1480255/150‑1 (MQ=255)
                      tAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGa                                                                                                                                  >  6:554218/1‑150 (MQ=255)
                       aacaacTGGGGGCACCGGCAGTTGCCATTAATTTAAAATAATAATTCCCCTTTTATTAAAATCTGATCACCTTCCAATAGCCTAATGCACCATCACTCATCATCTTAATTATTTTTGGGGGAGGCGGCGGGGGGCGGATAACTTTCGGAAt                                                                                                                                <  3:754404/151‑1 (MQ=255)
                       aaCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt                                                                                                                                >  2:646562/1‑151 (MQ=255)
                          aaaTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGAGAACTTTCGGAATAGc                                                                                                                             >  2:1607303/1‑151 (MQ=255)
                              gggggCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCgg                                                                                                                          >  8:706363/1‑150 (MQ=255)
                               ggggCACGGGCAGGTGCCATTCATTTAACATCATATTTCCCCTTTTTTGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGATGGATATTTGGGGGCGGCGGAGGGGGGCGGATAACTTTCGGAATAGCCGGAt                                                                                                                        <  4:1646978/151‑1 (MQ=255)
                                 ggCACCGTGAGGTTCCATGCATTTAACATCAAAATTCCCCTGTTATGAAAATCTCATCACCTTCCCATACCCTAATGCAGGATCACTCATCATCTTAAGGTTATTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCa                                                                                                                      <  8:1546948/151‑1 (MQ=255)
                                   cACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAgg                                                                                                                    >  7:627719/1‑151 (MQ=255)
                                    aCCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAgg                                                                                                                    >  6:1100633/1‑150 (MQ=255)
                                    aCCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAgg                                                                                                                    >  5:557980/1‑150 (MQ=255)
                                    aCCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCCGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAgg                                                                                                                    >  8:1127263/1‑150 (MQ=255)
                                      cGGCAGGTGCCATTCATTTAACATAATAATTCCCCTGTTATTAAAATCTGATCACCTTTCCATACCCTAATGCAGCATCACTCATCATCTTAAGTATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAtt                                                                                                                 <  2:801471/151‑1 (MQ=255)
                                      cGGCAGGTGCCATTAATTTAACATAATAATGCCCCTTTCATGAAAATCTTATCACCTTCCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATGTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAtt                                                                                                                 <  3:488891/151‑1 (MQ=255)
                                        gactgttcaaTGCATTTAAAATCATAATAACCCTGCGATGAAAATCTTATGCCCTTGCCATCCCCTAATGCAGGACAACTCATCCTCTTCCTTTTCTTTGGGCGAGTAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtc                                                                                                               <  5:163382/142‑1 (MQ=255)
                                          aGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGCGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcct                                                                                                             >  7:436727/1‑151 (MQ=255)
                                            gTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcctcc                                                                                                           >  4:1368502/1‑151 (MQ=255)
                                             tGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTcctcct                                                                                                          >  1:744869/1‑151 (MQ=255)
                                              gacattcattTAACATAATAATTCCCCTGTGATGAAAATCTGATCCCCTTCCAATCGCCTAATGCAGGATCACTCATCATCTGAAGGATTTTTGGGGGCGCCGGCCGCCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTg                                                                                                         <  1:354342/149‑1 (MQ=255)
                                              gCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTg                                                                                                         >  4:892060/1‑151 (MQ=255)
                                               ccatccacatAACAACAAATTTCCCCTCTGATGATAATCTGACCTCCTGCCCAACGCCACCTCCACGATCACTCATCTTCTGTCGGATCTTTGGGCGCCGAGCCCGCCCGCGGAGACCTGTCGGAATAGCCGGATCAGGATTTCCTCCTGa                                                                                                        <  6:848141/142‑1 (MQ=255)
                                               ccATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTg                                                                                                         >  5:66390/1‑150 (MQ=255)
                                               ccATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTg                                                                                                         >  2:1462357/1‑150 (MQ=255)
                                                aattcATTTAACATCATAATTCCCCTGTGATGAAAATCTGCTCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGaa                                                                                                       <  6:999958/147‑1 (MQ=255)
                                                  ttaaTTTAACATCATAATTCCCCTGTGTTTTAAATCTGATCACCTTGCTATCGCCTAATCCCGGTTCACTCCTCTTCTTAATTTTATTTGGGGGTGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAc                                                                                                      <  2:462860/147‑1 (MQ=255)
                                                   gCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCg                                                                                                    >  1:260466/1‑151 (MQ=255)
                                                   gCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCg                                                                                                    >  5:1435764/1‑151 (MQ=255)
                                                   gCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCg                                                                                                    >  4:691456/1‑151 (MQ=255)
                                                        tAACATCATAATCCCCCTGTCATCAAAAGCTGATCACCTTCCCATAGCCTAATGCAGGATCCCTCATCATCTGAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGAGCAGGATTTCCTCCTGAACCGGCtt                                                                                                <  6:1692753/150‑1 (MQ=255)
                                                          aaataatAATTCCCCTGTTATCAAAATCTGATCACCTTGCGATACCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGCGGCGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACa                                                                                             <  4:371667/146‑1 (MQ=255)
                                                          aCATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc                                                                                              >  2:720307/1‑150 (MQ=255)
                                                            aacaaaaTTCCCCTGGGATCAAAATCTCAACACCTTCCCATCCCATAATGCCTCACCACTCATCATCTGAAGGATCTTTGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGa                                                                                           <  8:172083/146‑1 (MQ=255)
                                                             aaatAATTCCCCTGTGATCAAAATCTCATCACCTTCCCATAGCCTAATGCAGCATCACTCATCATCTGAAGGATTTTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGaa                                                                                          <  2:1145073/149‑1 (MQ=255)
                                                                tAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGc                                                                                       >  5:486328/1‑151 (MQ=255)
                                                                 aaTTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGc                                                                                       >  7:1680346/1‑150 (MQ=255)
                                                                  aTTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCa                                                                                      >  4:1263689/1‑150 (MQ=255)
                                                                   ttCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACATTCGGAATAGCCGGATCAGGAATTCCTCCTGAACCGGCTTACAGAACGCa                                                                                      >  6:248534/1‑149 (MQ=255)
                                                                     ccccTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAgg                                                                                   >  3:1263698/1‑150 (MQ=255)
                                                                       ccTTTGATGAAAATCTGAACACCTTCCCATACCCTAACGCAGGATCACACATCATCTCAAGGATATTTGGGGGAGGAGGCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGAtt                                                                                <  5:1132884/151‑1 (MQ=255)
                                                                         tgtgATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTAc                                                                              >  3:1780415/1‑151 (MQ=255)
                                                                          gtgaacaaaaTCTGAGCACCTTGCCATACCCAACTCCCCGATCACTCATCATCTGAAGGATAGTGGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTa                                                                               <  5:89240/143‑1 (MQ=255)
                                                                            gatgaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTgg                                                                           >  1:928510/1‑151 (MQ=255)
                                                                             atgaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGt                                                                          >  3:995239/1‑151 (MQ=255)
                                                                              ataaaaTCTGATCACCTTCCCATAGCCTACTGCAGGATCACTCATCATCTCACGTATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtt                                                                         <  2:1667673/149‑1 (MQ=255)
                                                                               gaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGttt                                                                        >  5:349838/1‑151 (MQ=255)
                                                                                aaaaTCTGCTCACCTGGCCATAGCCTAAGGCAGGAGCACTCATCATCTGCAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtttt                                                                       <  1:1496722/151‑1 (MQ=255)
                                                                                aaaaTCTGATCACCTTGCCATAGCCTAATGAAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGCGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCCGCTTACAGAACGCAAGGATAACTGGttat                                                                       >  8:1279697/1‑149 (MQ=255)
                                                                                aaaaGCTGAGCACCTTGCCATACCCAAATGCCGGATCACTCCTCATCGGAAGGATATTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtttt                                                                       <  3:1346669/151‑1 (MQ=255)
                                                                                  attctttttaCCTTGCCATAGCGTTTTGCAGTTTCATTCTTAATCTGTTGTTTTTTTGGTGGATGTGGTCGGGTGTGTATAACTTTCTGAATAGGGTGATCAGGATTTCGTGCTGAACCGGCTTACTGAAGGCAAGGATTACTGGTTTTca                                                                     <  6:1250001/142‑1 (MQ=255)
                                                                                       gcgcACCTTGCCGTCGCGTCATGCAGGAGCACTCATCATCTGAAGGATCTTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcag                                                                    <  1:502709/144‑1 (MQ=255)
                                                                                       gaccACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagc                                                                   <  5:1316449/145‑1 (MQ=255)
                                                                                       gATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagcc                                                                  >  4:835974/1‑149 (MQ=255)
                                                                                       gATCACCTTCCCAAACCCTACGGCAGCATCCCTCATCATCTTAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagc                                                                   <  4:289/148‑1 (MQ=255)
                                                                                        aTCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTcagccagc                                                               >  1:430249/1‑151 (MQ=255)
                                                                                         tCACCTTCCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCACCATTTCCTCGTGAACCGGCTTACAGAACGCAAGGATAACTGGTTTTCAGCCAGCt                                                              >  2:186121/1‑151 (MQ=255)
                                                                                          cACCTTGCCATAGCCTAATGCAGGATCACTCATCAGCTGAAGGATTTTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTg                                                             <  7:1208834/151‑1 (MQ=255)
                                                                                           cccTTGCCATAGCCTCATCCAGGAACCCTCATCATCTGACGGATATTGGGGGGCGGCGGCCGCGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGa                                                            <  1:1794401/150‑1 (MQ=255)
                                                                                            ccTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGAc                                                           >  5:1704940/1‑151 (MQ=255)
                                                                                            ccTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTGGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGAc                                                           <  3:704944/151‑1 (MQ=255)
                                                                                            ccTTGCCATACCCTAATCCTGGTTCACTCATCATCTGAAGGTTTTTTGGGGTCGGTGGAGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGAc                                                           <  2:1071258/151‑1 (MQ=255)
                                                                                                ccaaTACCCTAATGCAGGATCACTCATCATCTGAAGGATTTTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATc                                                       <  8:764946/148‑1 (MQ=255)
                                                                                                 ccctcgCCTAAGGCAGGATCCCGCAACATCTGCCGGATATGGGGGGGCGGCCGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCg                                                      <  1:83688/146‑1 (MQ=255)
                                                                                                     aGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggc                                                  >  7:528872/1‑151 (MQ=255)
                                                                                                          aatcccggATCACTCATCATCTGATGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGgcggcg                                              <  7:1794382/144‑1 (MQ=255)
                                                                                                          aaTGCAGCATCACTCATCATCTGACGTATATTTGGGGGAGGCGCACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcggc                                               <  5:1246232/149‑1 (MQ=255)
                                                                                                           aTGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGtt                                            >  1:1664939/1‑151 (MQ=255)
                                                                                                             ccagccccACTCAGCATCTGCAGGATCGGGGGGGGCGGCGCCCGGCCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTa                                          <  1:718055/144‑1 (MQ=255)
                                                                                                              cAGCATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTATAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTaa                                         <  7:232983/151‑1 (MQ=255)
                                                                                                                   tCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTAATGGTTTTCAGCCAGCTGACGATCGTTGCGGCGTTTAAACAt                                     >  4:1092566/1‑150 (MQ=255)
                                                                                                                     aCTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATcc                                   >  1:1155019/1‑150 (MQ=255)
                                                                                                                         agcatcTGAAGGATATGGGGGGGCGGACGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCa                              <  1:1011804/149‑1 (MQ=255)
                                                                                                                         agaatcTGAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCa                              <  3:1561444/148‑1 (MQ=255)
                                                                                                                           catcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAAt                            >  4:1743411/1‑151 (MQ=255)
                                                                                                                            atcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATc                           >  5:195442/1‑151 (MQ=255)
                                                                                                                            atcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATc                           >  3:1161741/1‑151 (MQ=255)
                                                                                                                              gtGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCAAGCTGACGATCGTGGCGGAGTTTAAACATCCAGCCAATCa                          >  7:1617677/2‑150 (MQ=255)
                                                                                                                                  aGGATATTGGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGc                     <  8:250284/151‑1 (MQ=255)
                                                                                                                                   ggAGATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTGCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCACCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGc                     >  1:1086169/1‑150 (MQ=255)
                                                                                                                                        tttGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaac                >  8:366893/1‑150 (MQ=255)
                                                                                                                                         ttGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaaca               >  8:1000984/1‑150 (MQ=255)
                                                                                                                                            ggggCGGCGGCCGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATcc           <  8:861175/151‑1 (MQ=255)
                                                                                                                                             gggAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGCCGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCCCAACACTccc          >  6:740982/1‑150 (MQ=255)
                                                                                                                                              ggccgaggcCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAc         <  1:644074/147‑1 (MQ=255)
                                                                                                                                              ggaggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAc         >  2:1753161/1‑151 (MQ=255)
                                                                                                                                              ggaggaggaCGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAc         <  7:878683/151‑1 (MQ=255)
                                                                                                                                               gaggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAAAAATCCAcg        >  2:905950/1‑151 (MQ=255)
                                                                                                                                                  gaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAcgcg      >  8:1217793/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGAAAC  >  DH1/3448376‑3448675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑