Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,906,8370CG29.1% 162.7 / 26.7 110intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base C (59/14);  new base G (30/0);  total (95/15)
Fisher's exact test for biased strand distribution p-value = 9.02e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GGGGTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTT  >  DH1/3906688‑3906987
                                                                                                                                                      |                                                                                                                                                      
ggcccgccactacccccccTCGGAGCTAAAAACTCGTTTATATTGTT‑GAGTGTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCg                                                                                                                                                       <  3:893298/139‑1 (MQ=14)
 gcgggcAAGGCCCCCGCCTCGCAGCTAACACGACTTTTTTATTTCT‑CGGTGTAGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGa                                                                                                                                                      <  5:300482/146‑1 (MQ=14)
          gacccccccTCGCACCAAACACCACGTTTATATTGAT‑CTGACTCTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCaaaaa                                                                                                                                             <  7:1320905/149‑1 (MQ=18)
            cccccccTCGCACCAAAAACCAAGTTGATATTTCT‑AGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGTGGGTTAAAATAAACCCCCGCGGACCAAAAGAGGGTATATGaataaat                                                                                                                                           >  8:641491/1‑150 (MQ=18)
               ccccTCGCACCAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGAGGAATTGGTAGACGAGCTAGCTTCAGGTGTTAGTTTACTTACGGACGTGGGGGGTAAAGTCCCCCCCCACGCACCAAAAGAAGGTATATGAAAAAAAGtt                                                                                                                                        >  2:1678773/1‑150 (MQ=255)
                cccTCGCACCAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTGCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCCGCCCCAAACACGCCTAAATCaataaaactt                                                                                                                                        >  1:1411801/1‑147 (MQ=18)
                   tCGCACCAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAAATCCAAAAAAGTa                                                                                                                                        >  3:902703/1‑147 (MQ=255)
                    cGCACCAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTGAACTCCCCCCCCGCGCCCCAAAAGAGGGTAAATCaaaaaaatttatatt                                                                                                                                   >  7:593281/1‑143 (MQ=18)
                     gCACCAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAATCAGGCTAGATCCAAAAATGTTAGTTgc                                                                                                                                  >  3:871681/1‑150 (MQ=18)
                     ccACCAAACACCACGTTGATAGTCCT‑CCCCCTGGGCGAAGGGGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGGCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGa                                                                                                                                  <  8:1517417/150‑1 (MQ=21)
                      cACCAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGTAATTCGTAGACGCGCTAGCTTCAGGTGTTAGTCTTATTACGGAAGTGGGGGATAAACAACCCCCCCAAACACCAAAAGAAGCACAATACAAAAAACTAACATGa                                                                                                                                  >  7:44181/1‑150 (MQ=16)
                      cACCAAAAACCACGTTGATATTGCC‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACGTTAAATCAAATAAAGtttgttta                                                                                                                                  >  6:969639/1‑143 (MQ=17)
                       aCCAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGACGTTAGATCAAATAATGTATGAtccat                                                                                                                                >  5:117047/1‑147 (MQ=18)
                       aCCAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACTAAAAATCAATAAAAGTTAGATgcat                                                                                                                                >  2:328002/1‑148 (MQ=17)
                        ccAAACAACACGTTTATATAGATGCGC‑CTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGAc                                                                                                                                 <  7:1732210/149‑1 (MQ=17)
                        ccAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGTATATCAAATAAAGTTTGATGACtg                                                                                                                               >  3:921168/1‑151 (MQ=18)
                        ccAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACGATATATCAAATAATGTTATATGACtt                                                                                                                               >  3:1556490/1‑150 (MQ=18)
                         cAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATAAAGTATATATCAAAAAAAGTATGATGAgtgt                                                                                                                              >  6:1668560/1‑151 (MQ=18)
                         cAAAAACAACTTTTATATTGCT‑CCCAGTGGGCGAAGGTGGCGGAATTGGTAGCCGCGCTAGCTTCAGGTGTTAGTGTTCTTCCGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTACGATGACtgt                                                                                                                              <  7:713493/151‑1 (MQ=21)
                           aaaaCCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAGGAGGGGAGATCCAAAAATGTATGATACCtgtgg                                                                                                                            >  5:1313541/1‑150 (MQ=18)
                           aaaaCCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGAACGATAGATCAAAAAAAGTTTGATgcatgttt                                                                                                                            >  5:1028680/1‑144 (MQ=17)
                                     tGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTGAAGTCCCCCCCCGCGCACCAAAGGACGGGAAATCAAATAAAGTTTGTTGAGTGTGTGTTGGTGGCg                                                                                                                  >  1:1474856/1‑151 (MQ=17)
                                      gATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAATTCGCGAAATCATAAAATGTTTGTTGACTGTGTGTTGCTGGCgg                                                                                                                 >  3:735446/1‑151 (MQ=14)
                                        tatTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGTATATCAAAAAAAGTTTGTTGACtgttatttgctggcgga                                                                                                                >  8:384959/1‑136 (MQ=17)
                                        tatTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCACCAAACGTGGGTAGATCAAAAAAAGTTAGTTGACTGTTTTAAGGTGGCGGat                                                                                                               >  5:655828/1‑150 (MQ=18)
                                        taattcttgctagttggCGAAGGGGGCGGATTTGGAAGAGGCTCTAGCTTGAGGTGGTTGTGTTATTACGGACGTGGCGGTTCAAGTCCCACGCCTCGCACGAAACGCGACGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCgg                                                                                                                 <  3:1496830/135‑1 (MQ=255)
                                         agaccT‑CGCACTGGGCGAAGGTGTCGGAATTGGTAGACGCGCTAGCTTCAGCTGTTTGTGTTCTTCAGGAGTTGGGCGTGCTAGTCCCACCCCTCCCACTAAACGAGCCGAAATCAAATAAAGTAATATGACTGTGCGAAGGTGGCGGAAt                                                                                                              <  7:379884/147‑1 (MQ=21)
                                           tGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCCAACGAGGCGATTTCAAAAAAAGGTAGATGACTGTGGGTAGGTGGCGGAATTg                                                                                                            >  3:268322/1‑151 (MQ=21)
                                           tGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGGTATTTCAAATAAAGTTTGTTGCCTGTTTTTAGGTGGCGGATTTg                                                                                                            >  1:575189/1‑151 (MQ=14)
                                              t‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAGAAGGGTAAATCAAATAATGTTTGATGACTGTGTTTAGCTGGCGGAtttctat                                                                                                         >  7:455995/1‑147 (MQ=14)
                                                gcacggttgtgAAGGTCTCGCAATTGGTAGACCATATAGCTGGAGGTGTTTGTTTTATTAATGACGTTGCGGTTCAAGGCCCACGCCTCCCACCAAACGCGTCGATATCAAAAAAAGTAATCTGACTGTGCGAAGGTGGCGGAATTGGTa                                                                                                         <  3:1143701/140‑1 (MQ=255)
                                                       ggCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAATCAGGGGAAATCCATAAAAGTTTCATGCATGTGGGTTGCTGCCGGAATTGGTataatttt                                                                                                  >  1:544173/1‑143 (MQ=11)
                                                         cGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAATAGGGGAAATCAAAAATAGTAAGATGCCTGTTGTTAGCTGGCGGATTTGTTatccgaatg                                                                                                 >  7:1461997/1‑141 (MQ=14)
                                                           aaGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGTGGGGATATCAAAAAAAGTAAGATGCCTGTTGGAAGGTGGCGGAATTGGTAGAAGGGCTAGCt                                                                                             >  1:618441/1‑151 (MQ=21)
                                                               tGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCCAAAAAAGTTTGATGACTGTGGGTTGGTGGCGGAATTTCTATCCGCGGTAGCTTc                                                                                           >  5:1805847/1‑149 (MQ=17)
                                                                   ggAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGTGTATTTCAAAAATATTAACTTGACTTTGGGAAGGTGCCCGAATTGCTAGACCGGCAAGGTTCACgtgt                                                                                     >  3:385473/1‑151 (MQ=14)
                                                                   gccacccctcgACCCGCCAGCGGCAGGTGCCAGTGGTCGTCCGGCCGGGGCGGTGCAAGTCCCCCCCCACCCACCAACCGCCGCGAAATCTTAAAAAGTTCGATGACTGTGCGAAGGTGGCGGAATTGCTAGACGCGCTAGCTTCAGgtgt                                                                                     <  7:1288307/141‑1 (MQ=12)
                                                                          gTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTGAAGTCCCCCCCCAAGCACCAAACAACGCGAAATGAAAAAAAGTTATATGAATGATAGAAAGTGGCGGAATTGGAATAAGCGCGAGCATCAGGTGTTAatgt                                                                               >  7:983504/1‑150 (MQ=12)
                                                                          gTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGCCTGTGGGAAGGTGGCGGATTTGGTAGCCGGGCTAGCTTCAGGTGTTAGTGTc                                                                              >  1:305024/1‑151 (MQ=255)
                                                                           tAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGGGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAgtgtac                                                                             >  5:534830/1‑149 (MQ=255)
                                                                            ccccgcgCTCGCGTCAGCTGCTAGTGTTCTTCCGGACGTGGTTGTTTTTTTTCAAGACCTCGCACCAACCGATTTTATATCATATATAGTAAGATTACTGTTCGAAGTTGGTGTTATTTGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCt                                                                            <  8:673410/148‑1 (MQ=255)
                                                                                gcgcTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCCCCAAACCACGGGATATCAAAAAAAGTAAGATGACTTTTCGTACGTGGCGGAATTGGTATAAGCGCTAGCTTCAGGGGTTATTGTCCGTAcc                                                                        >  7:92512/1‑150 (MQ=21)
                                                                                  gcTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGAGTTCAAAAAAAGTAAGATGCATGTTGGTTGGTGGCGGATTTGGTATACTCGCTAGCATCAGGGTTTAgtgtagtaaaggc                                                                      >  6:1654662/1‑142 (MQ=18)
                                                                                   cTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTGAAGTCCCCCCCCACCCACCAAAAAAGGGGAATTCAAAAAAATTTTGTTGACTTTTGGTAGGTGGCGGAAATTGGAGACCCCCCACCGTGACGGGGTAGTGTAATTACGGAc                                                                     >  5:585023/1‑151 (MQ=2)
                                                                                     aGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGTATTTCAAAAATAGTAAGATGAGTGTTGGATCGTGGCGGAATGGGTATAAGTGTGAGCTTCAGGTTTTAttgtaattttggccgg                                                                   >  1:1120182/1‑139 (MQ=12)
                                                                                     aGCTTCAGGTGGTAGGGTTCTTACGGACGTGGGGGGTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGCTGACTGTGCGAAGGTGGCGGAATTGGGAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGAc                                                                     <  3:1502766/149‑1 (MQ=255)
                                                                                      gCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCACCAAACGAGGGGATATCAAAAAAAGTTAGATGCCTGTTGGTAGGTGGCGGAATTGGTATCCTGGCTATCATCAGGTTTTATTGTACTTACGGCCGTg                                                                  >  7:1688314/1‑151 (MQ=21)
                                                                                       cTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGTATATCAAAAAAAGTTAGTTGACTGTGGTTAGGTGGCGGAATTTCTATAAGAGGTATCTTCAGGTTTTAgtgtaaataaggccgtgg                                                                 >  5:1610360/1‑137 (MQ=12)
                                                                                          cAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGAc                                                                     <  2:781082/144‑1 (MQ=255)
                                                                                          cAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGGAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGGGTTAGTGTCCGTTCGGAc                                                                     >  1:778586/1‑144 (MQ=255)
                                                                                           aGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGTGGGGATATCAAATAATGTTTGTTGCTTGTTGTATGATGGCGGAATTGGTATAAGTTTAATCTTCAGGTTTTAGTGTACTTACTGACGAaggggg                                                             >  1:1113718/1‑150 (MQ=9)
                                                                                                   gtgtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGTATATCCAATAATGTTAGTTGCCTGTTGTTTGGTGGCGGAATTGCTTGAAGTGCAATCTTCAGGTGTGAGTGAAATTAAGCACGGGGGGGTTCAAggc                                                      >  1:1256845/1‑148 (MQ=12)
                                                                                                        cTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGATATCAAAAAAAGTAAGATGCGTGTGCTAAGCTGGCGGAATTGGTATACGCGCTAGCTTCAGGTTTGAGTGTCCTAACGGACGGGGGGGTTGAAGGcccccgc                                                >  7:589854/1‑149 (MQ=25)
                                                                                                         ttACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAGGGGAGATCAATAATAGTATGATGCATGATGTATGGTGGCGGAATGGGATTACTAGGTAGCATCATGAGTGAGTGAAGATAAGGACGTCGGGGTTCTAgaaaaaacg                                                >  6:927205/1‑142 (MQ=2)
                                                                                                          tACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAACAGGCGATATCAAAAAAAGTATGATGAATGTGGGTAGGTGGCGGAATTTGTATAAGTGGGATCATCAGGTTTTAGTGAAATTAAGGACGTggcgggtgtaggaaaaaccc                                               >  1:909736/1‑135 (MQ=16)
                                                                                                                cGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGAGATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGATAGCTTCAGGTGTTAGTGTCGTTCCGGACGTGGGGGTTCAAGGCCCCCCCCTCGCAcc                                        >  1:1276169/1‑151 (MQ=255)
                                                                                                                 gTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGCCTGTGGGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCGTTACGGACGTGGGGGTTGTAGGccccgcccgaggccc                                        >  7:1202944/1‑143 (MQ=25)
                                                                                                                  tGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGCGATATCAAAAAAAGTAACATCAATGTTATAACATCCCGGAATTAATATAATAGATAGCTTCAGGTGTTAGTGTCATTAAGGCCGTGGCGGTTCAAGTACCCCGCCTCGccgca                                       >  1:24079/1‑146 (MQ=255)
                                                                                                                       gTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGGTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGTCCCCGCCCTCGCCCCAATTAtg                                 >  5:842076/1‑150 (MQ=255)
                                                                                                                        ttCGAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGGGAAGTTGGCGGATTTGGTAGACGCGCTAGCTTCAGGTGGTAGTGTCATTACGGCCGTGGGGGTTCAAGT‑ACCCCCCTCGCCCCAATTTTCtt                               >  1:1618545/1‑151 (MQ=21)
                                                                                                                        ttCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAATAAAAGTAAGATGAGTGTGCGAAGGTGGCGGAATTGGTAGACGGGGTAGCTTCAGGTGTTAGTGTCCTTACGGCCGTGGGGGTGGAAGGCCGCCGCCTGGCCCCAATTAtgt                                >  3:1629029/1‑149 (MQ=21)
                                                                                                                        ttCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGCATGTGGGAAGGTGGCGGATTGCGTAGACGGGCGAGCTTCAGGTGGGAGTGTCGATAGGGACGTGGGGGGTGAAGGaccaccccgtgccgcaagtgtgt                                >  8:676760/1‑136 (MQ=255)
                                                                                                                         tCAAGTCCCCCCCCTCGCACCAATCGAGGCGAGATCAATTAAAGTAAGTTGCCTGTGCGATGGTGCCGGATTTGGAAGAAGCGCTAGCTGCAGGTGGTCGTCTCCTTAGGGACGTGACGGTGGATGTCCAGCCCCACGCACCacatgtgtg                               >  4:530728/1‑143 (MQ=255)
                                                                                                                          cAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGCCTGTGCGAAGGTGGCGGAATTGGTAGACGCGGGAGCTGCAGGTGTTAGTGTCGTTAGGGACGTGGGGGTTCAAGGACCACCCCAGGCCCCAATTTTAttt                              >  1:861334/1‑151 (MQ=21)
                                                                                                                            aGTCCCCCCCCTCGCACCAAACGAGGCGAGATCAAAAAAAGTAAGATGACTGTGCGAAGGTCGCGGAATGGGTAGAAGCGGTAGCTTCAGGTGTTAGTGTCCTTCCGGACGGGGGGGTTCTAGTCACACCCAACGCACCAATTATGtttt                             >  3:87005/1‑149 (MQ=21)
                                                                                                                             gTCCCCCCCCTCGCACCAAAGGAGGGGAGATCAAAAAAAGTATGATGAGTGTGGGAAGGTGGCGGAATTGGTAGACTGGGTAGCTTCAGGTGGTAGTGTAGTTACGGACGTGGGGGTTGAAGTGCCCCGCAGCGCACCAATCTTATTTACt                           >  5:1705288/1‑151 (MQ=16)
                                                                                                                               ccccccccTCGCACCAAAAGAGGCTATATCAAAAAAAGTAAGATGACTGTTAGAAGGTGGCGGAATTGCTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGAAGTGGGGGTTCAAGTAACACCCCAAGCACCAAATATGttttatt                          >  8:620717/1‑146 (MQ=18)
                                                                                                                                 ccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCt                       >  5:1445323/1‑151 (MQ=255)
                                                                                                                                  cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCtt                      >  5:1857810/1‑151 (MQ=255)
                                                                                                                                  cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCtt                      >  1:51114/1‑151 (MQ=255)
                                                                                                                                  cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAATATATTTAATTCCtt                      >  2:564299/1‑151 (MQ=255)
                                                                                                                                  cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAGTCCCCCCCCGCGCCCCAATTATGTTTTCTTGCtt                      >  5:510899/1‑151 (MQ=21)
                                                                                                                                   ccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCttt                     >  5:1078889/1‑151 (MQ=255)
                                                                                                                                   ccccTCGCACCAAACGAGGCGATATCAAAAAAACTAACAAGACTGAGCGAAGGTCGCGGAATTGGTNGANNNNNTANCTACAGGAGTTACTGTCCTAACGGACGTCGCGGTTCAAGTCCCCGGCCACGCAACAAATaaatatacatccat                      >  2:957663/1‑137 (MQ=255)
                                                                                                                                    cccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTAATTACttt                     >  4:1273048/1‑150 (MQ=255)
                                                                                                                                     ccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTCGGGGTTGAAGGCCCCCCCCTGGCACCAATTTTCATTACATCCtttat                   >  6:1432087/1‑149 (MQ=21)
                                                                                                                                       tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAATATGTTTAATTGCGttgttg                 >  6:1345604/1‑151 (MQ=18)
                                                                                                                                         gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGtt               >  6:1290330/1‑151 (MQ=255)
                                                                                                                                         gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTCTCCTTACGGACGTGGGGGTTCTATTCCCCCCCCTCGCCCCCATTTTATTTACTTCCGTTCTTGtt               >  7:1442751/1‑151 (MQ=21)
                                                                                                                                          cACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGttt              >  2:1388252/1‑151 (MQ=255)
                                                                                                                                           aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTGGCACCAATTATCTTTACTTCCTTTCTTGtttg             >  6:1778931/1‑150 (MQ=255)
                                                                                                                                           aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTGCTTTCTTGtttg             >  8:171105/1‑150 (MQ=255)
                                                                                                                                           aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGtttg             >  2:325668/1‑150 (MQ=255)
                                                                                                                                           aCCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGtttg             >  3:1336842/1‑150 (MQ=255)
                                                                                                                                            ccAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttct            >  7:1207425/1‑151 (MQ=255)
                                                                                                                                             cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGTttctt           >  3:194015/1‑151 (MQ=255)
                                                                                                                                             cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttctt           >  1:1615809/1‑151 (MQ=255)
                                                                                                                                              aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTGCCCCCCCTCGCACCAATTATGTTTACTTCCTTTCTtgtttgttg          >  2:531339/1‑150 (MQ=255)
                                                                                                                                              aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGTttcttc          >  3:617082/1‑151 (MQ=255)
                                                                                                                                              aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTttcttg          >  2:1479004/1‑150 (MQ=255)
                                                                                                                                              aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGGGAAGTGCCCCCCCGCGCCCCCACTATGTTTTCTTGCGTTGTttttttttg          >  7:446988/1‑150 (MQ=18)
                                                                                                                                               aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGTTTCTTcc         >  3:1284890/1‑151 (MQ=255)
                                                                                                                                               aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTcc         >  3:613441/1‑151 (MQ=255)
                                                                                                                                               aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGGGCCCCCCCTCGCACCAATTATCGTTACTTCCTTTCTTGTTTCTTcc         >  4:235894/1‑151 (MQ=255)
                                                                                                                                                aCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCt        >  8:918867/1‑151 (MQ=255)
                                                                                                                                                 cGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACATCCTGTCTTGTTTGTTCCtt       >  5:484093/1‑151 (MQ=255)
                                                                                                                                                  gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTGAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTGTTGTTTCTTCCTTg      >  5:628677/1‑151 (MQ=255)
                                                                                                                                                   aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTg      >  3:190403/1‑150 (MQ=255)
                                                                                                                                                   aGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAATTATGTTTAATTCCTTTCTTGTTTATTCCTTg      >  8:448094/1‑150 (MQ=255)
                                                                                                                                                      cGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGAttt  >  7:1497043/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GGGGTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCT‑CGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTT  >  DH1/3906688‑3906987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑