Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,122,1240CA22.5% 436.9 / 18.8 283intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (117/66);  new base A (51/2);  total (209/74)
Fisher's exact test for biased strand distribution p-value = 6.19e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

TCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121976‑4122272
                                                                                                                                                    |                                                                                                                                                     
aataaaaaCTCAGCAATTCCCGGTTGGGTGGTCTGGTCATTACAGCTATTTCATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCtt                                                                                                                                                     <  7:1471824/145‑1 (MQ=17)
 ctACAAACTCAGATTTTCCCTCGGGGGTGGTCTGTACCTTTTATTTCTTTAATAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCt                                                                                                                                                      <  6:1764542/149‑1 (MQ=11)
   acaaaCTTAGCTATTCCCCCTTGGGTGGTGTGTTCATTATATCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTgc                                                                                                                                                  <  8:1398607/148‑1 (MQ=17)
   aCCAACGCAGCTAATCCCTCGTGGTGGGTCTGTCCATTATATCAATATAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTgc                                                                                                                                                  <  5:203721/151‑1 (MQ=14)
          gAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCATGGCAAGGTCCTGCACGAACAACTGAGCGATTCCCCCTGGGGTCGTATGTGGTTAGCAGGACtt                                                                                                                                           >  6:724578/1‑151 (MQ=14)
           cccaattcgcccgtGGGCGGTCTGTTTATTATAGCAAGTTCATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACttt                                                                                                                                          <  6:1697015/138‑1 (MQ=14)
            gctaaacccGCCTCGGCGGTCTGGACATTACAGCACTAGAACATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACtttt                                                                                                                                         <  3:14074/145‑1 (MQ=14)
            gatATTCGCGCTTGGGGGGTCTGGTTCTTACAGCAATTTCATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACtttt                                                                                                                                         <  2:1810097/149‑1 (MQ=17)
             atataCCCGCTTCTGCGGTCTGTTAATTAAAGAAATTTAACATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTc                                                                                                                                        <  3:118311/150‑1 (MQ=14)
               aTTCGCTCTTGGTCGGTCTGTTTATTATATCACTTTAATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCaa                                                                                                                                      <  4:550576/151‑1 (MQ=14)
               aTTCGCTCTTGGGGGGTGTGTGCATTATATCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCTGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCa                                                                                                                                       <  8:153476/150‑1 (MQ=17)
               aTTCGCGCGTGGGTGGTCTGTTCCTTACAGAACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCaa                                                                                                                                      <  8:1022261/151‑1 (MQ=255)
               aTTCCCGCTTGGGTGGTCTGTGACTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTAGAAATCGCGACCCCGACCTTGGCAACGTCGTGCTATACCAACTGAGCTATTCCCGCGTGGGTGGTCAGTTTTTTGGAGGAcggtggtg                                                                                                                                      >  4:1182077/1‑144 (MQ=14)
                  cccGCTTGGTGGGTGTGTTTCTTATATGACTTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAAt                                                                                                                                    <  6:637125/150‑1 (MQ=17)
                  cccGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTTGTTTTAAGCACTTTTCAAAtt                                                                                                                                   >  8:1220997/1‑151 (MQ=255)
                  cccGCTTGGGGGGTCTGGGCCTTACAGCCCTTGCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAAtt                                                                                                                                   <  5:638810/151‑1 (MQ=255)
                    cGCTTGGGTGGTGTGTGCCTTACATCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGACTGTGCTTTGCAGCACTTTTCAAAtttt                                                                                                                                 <  8:1375818/151‑1 (MQ=255)
                     gCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATTTTTTTTTAAGAACTTTTCAAAttttt                                                                                                                                >  4:1434236/1‑150 (MQ=17)
                      ccggggAGGTCTGGGCCTTACAGCACGTGCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGGGGTCTGTGCTTTGCAGCACTTTTCAAATTTTgg                                                                                                                               <  5:853700/148‑1 (MQ=17)
                       ttGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTCGTCTGTTCTCAGCCGCAATTTTCCAATTTTgg                                                                                                                               >  5:1367799/1‑150 (MQ=14)
                        tGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTgg                                                                                                                               >  7:1029323/1‑149 (MQ=255)
                        tGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGGAGGTATGTTATATTAAGAAATTTTCAAAtttttg                                                                                                                               >  6:794240/1‑147 (MQ=14)
                         gggTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTAAGTTTGTTGCAGGACTTTTCAAATTTAGGAGc                                                                                                                            >  5:576810/1‑151 (MQ=17)
                            tggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGCggg                                                                                                                         >  8:674406/1‑151 (MQ=255)
                            tggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTCGTCTGTGATTTGCAGCACTTTTCTAATTTAGGAGAggg                                                                                                                         >  8:665462/1‑151 (MQ=18)
                             ggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTTGTGTGCAGGAATTTTCAAATTTTGGAGAgggt                                                                                                                        >  3:674280/1‑150 (MQ=17)
                              gtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTTTTTTGAAGCACTTTTCTAATTTTTGAGAggta                                                                                                                        >  5:769240/1‑148 (MQ=17)
                               tCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTCTGTGCTTTGCCGCACTTTTCAAATTTAGGAGCGGGaa                                                                                                                       >  6:576795/1‑150 (MQ=255)
                                gtgggcattACAGCACTTTAAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAAc                                                                                                                     <  7:189605/144‑1 (MQ=255)
                                  gtcactaccagccatttCATATTTTGCCGCGTAAAACGAGCCTCGACCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGGCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACga                                                                                                                   <  7:699865/137‑1 (MQ=14)
                                   tgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTAGTTGAAGCACTTTTCCAATTTTGGAGCGGGTAACg                                                                                                                    >  3:324444/1‑149 (MQ=17)
                                         aCAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCCGTCGTAAGTTATATGAAGAAATTTTCAAATTTATGAGACGGAAACGAGAcacc                                                                                                             >  6:511781/1‑147 (MQ=11)
                                             cACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGTTCTGTTATTTGAAGAACTTTTCAAATTTAGGAGACGGAAACgcgccacgatat                                                                                                         >  2:1452295/1‑139 (MQ=17)
                                             cACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGA‑TCt                                                                                                        >  4:1810580/1‑150 (MQ=255)
                                              tcgttCAAATTTTGGATCGGGAAACGAGCCTCGAAATCGCGACCCCGACCTTGGCAAGGTAGTGCGCTAACAACTGAGCTATTCCCGCTTGGGTGGGGTGTGATTTGCACCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTc                                                                                                       <  5:528290/147‑1 (MQ=14)
                                              aCTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGTTCTGTGATTTGCAGGACTTTTCAAATTTTTGAGGGGGTAACGAGACTCGA‑ACTcg                                                                                                      >  3:1091799/1‑151 (MQ=14)
                                              aCTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGGTGTTATTTTTTGTGAAGAAATTTTCAAATTTTTGAGAGGTAAACGAGAcaccatcta                                                                                                        >  4:1297668/1‑142 (MQ=14)
                                                  tCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCACCTGAGCTATTCCCGCGTGGGTGGTCTGTGGGTTGGCGCACTTTTCCAATTTTTGAGCGGGAACCGAGCGTCGA‑TCTCGCGAc                                                                                                  >  3:646112/1‑151 (MQ=14)
                                                  tCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTATGTTATGTGGCGGAATTTTCTAATTTTTGAGCGGGAACGGAGGGTGGA‑TCTCGCGAc                                                                                                  >  1:1100488/1‑151 (MQ=14)
                                                   cAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGGGTTGACGCACTTTTCAAATTTTGGAGAGGGTAACGCGACTGGA‑ACTAGCGAcc                                                                                                 >  4:774566/1‑151 (MQ=14)
                                                             aGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGATTTGAAGGAATTTTCAAATTTTTGAGAGGGAAACGAAGCTCGA‑ACTCGCGACACCGAACtacg                                                                                       >  6:1583116/1‑148 (MQ=14)
                                                                   aaaCGCGACTCGAACTCGCGTCCCGGACCTTGGCATGGTCGTGGTCTCCAAACGGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGt                                                                                 <  7:25181/151‑1 (MQ=14)
                                                                   aaaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGA‑ACTCGCGACACCGACGTTGGACAGGt                                                                                 >  4:999681/1‑151 (MQ=14)
                                                                    aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGAGGGAACGGAGACTCGA‑ACTAGCGACCCCGACATAGGCAAggac                                                                                >  7:173759/1‑149 (MQ=14)
                                                                        agaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTAGCGACCCCGACCTTGGCAAGGTCGTGc                                                                            >  3:303308/1‑151 (MQ=38)
                                                                         gaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGACGTAAACGAGACTCGA‑ACTAGCGACCCAGAACTACGAAAGGTCCTGc                                                                            >  6:845481/1‑150 (MQ=14)
                                                                           cTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGGAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGA‑ACTCGCGACCCCGCCCTTGGCAAGGTCGTGctct                                                                         >  5:1743929/1‑151 (MQ=255)
                                                                           cTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTTGAGCACTTTTCAAATTTTGGAGCGGTAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGCTCGTCctct                                                                         >  7:1198615/1‑151 (MQ=11)
                                                                            tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGCAGGACTTTTCAAATTTTGGAGCGGGAAACAAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGctct                                                                         >  1:519720/1‑150 (MQ=255)
                                                                              gAACTCGCGACCCCGAACTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTCATTTATTTGCAGCACTTTTCAAATTTAGGAGAGGGAAACGAGACTCGA‑ACTCTCGACCACGAAATTGGAAAGGTCGTGcacaaa                                                                       >  8:1000043/1‑145 (MQ=14)
                                                                                     cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTCTTATGTGGTGTTACGAAATTTTCACATTTAGGCGAGGTAAACGA‑ACGCCACAAACGAAACCCCTCCATTAGCAAGGTAGTGCTCAACCAACTGa                                                                >  6:1861489/1‑150 (MQ=2)
                                                                                      gACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTACCAACTGAGc                                                              >  3:277813/1‑151 (MQ=38)
                                                                                      gACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAgg                                                              >  3:799821/1‑150 (MQ=38)
                                                                                         cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAACCGAGACGCGA‑ACTCGCGACCCCGCCCTTGGCAAGGTCGTGCGCTACCAACTGAGCTAt                                                           >  3:932881/1‑151 (MQ=255)
                                                                                          ccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGTTCTGTGCTTTGCAGGACTTTTCAAATTTTGGAGCGGTAAACGAGCCTCGA‑ACTCGCGACCCCGACCTTGGCAAGGACGTGCTCTAACAACTGAGCTAtt                                                          >  5:1530294/1‑151 (MQ=14)
                                                                                           cGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTc                                                         >  8:710282/1‑151 (MQ=38)
                                                                                            gACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGTTGGTATGTTATATTAAGCAATTTTCAAATTTATGAGAGGGAAAGGAGACACGA‑TCTAGCGACCACTCAGTTCCGAAGGTCCTCCGCAAACAACTGAGCTATTcc                                                        >  8:1794826/1‑151 (MQ=2)
                                                                                               cTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCAATTTTCAAATTTTGGAGAGGGAAACGAGACTCGA‑ACTCGCGACCCAGACCTTGGCAAGGTCGTCCTCTAACAACTGAGCTATTccagg                                                     >  8:28601/1‑148 (MQ=14)
                                                                                               cTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCACGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCcgc                                                     >  1:746211/1‑151 (MQ=255)
                                                                                                gaggcaccgTCGGGCTCAACCAACTGCGGTATTGCCGGGGGGGTGGGCGGGGGTCTCCAGCAATTTTAAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCcgcg                                                    <  7:381648/143‑1 (MQ=11)
                                                                                                  ggCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGt                                                   >  7:1297851/1‑150 (MQ=40)
                                                                                                   gCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTAGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGtac                                                 >  3:421383/1‑151 (MQ=255)
                                                                                                   gCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGACGGACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACATTGGCAAGCTCGGGCTCTACAAACTGAGCTATGCCGGCGta                                                  >  6:957691/1‑150 (MQ=14)
                                                                                                    cAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGGACTTTTCTAATTTTGGAGGGGGAAACGAGCCTCGA‑ACTCGCGACCCCGCGCTAGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGtact                                                >  3:1377508/1‑151 (MQ=14)
                                                                                                     aaGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTGGGGAAGGTCGTGCTCTACAAACTGAGCTATTCCCGAGtactc                                               >  5:1012003/1‑150 (MQ=255)
                                                                                                     aaGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGATGTGCAGCACTTTTCAAATTTTGGAGGGGGAAACGAGACTCGA‑ACTCTCGACCCCGACCTTGGCATGGTCGTGCTCTAACAACTGAGCTATTCCCGAGtagg                                                >  2:695165/1‑148 (MQ=14)
                                                                                                         tCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACtt                                            >  1:1484885/1‑150 (MQ=255)
                                                                                                           gTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACACCGACCTTGGCAAGGTCGTGCGCTACAAACTGAGCTATTCCCGCGTACTACTAAAt                                         >  7:461653/1‑151 (MQ=255)
                                                                                                           gTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGAGGGAAACGAGACTCGA‑ACTCTCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCAGCGTACTACTTAAt                                         >  3:1752828/1‑151 (MQ=255)
                                                                                                           gTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTGATGTGGAGCACTTTTCAAATTTTGGAGAGGGAAACGAGACTCGA‑AATAGCGACCCAGAAATAGGCCAGCTCGTGCACTACAAACTGAGATATTCCGGCGTAGTACTTAAt                                         >  2:292749/1‑151 (MQ=14)
                                                                                                             gCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑AGTAGCGACCCCGAGCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAAttt                                       >  3:489072/1‑151 (MQ=255)
                                                                                                             gCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAAttt                                       >  1:972548/1‑151 (MQ=255)
                                                                                                                ctACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTGTTTGCAGTAATTTTCAAATTTTGGAGAGGGAAACGAGACTCGA‑ACTCGCGACAACGACCTTGGCAAGCTCGTCCGCTACAAACTGAGCTATTCCCGCTTACTACTTAATTTTg                                     >  1:1548069/1‑150 (MQ=17)
                                                                                                                ctACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTACCAACTGAGGTATTCCCGCGTACTACTTAATTTTGc                                    >  5:1581447/1‑151 (MQ=255)
                                                                                                                ctACCAACTGAGCTATTCCCGCTTGGGAGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCACGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGc                                    >  4:619291/1‑151 (MQ=255)
                                                                                                                 tACCAACTGAGCTATTCCCGCTTGGGTGGTCTTTGATTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCAGACCTTGGCAAGGTCGTGCTCTAACAACTGAGCTATTCCCGAGTAATACTTAATATTGCt                                   >  2:976750/1‑151 (MQ=14)
                                                                                                                  aCCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTGTTGAGGAATTTTCAAATTTTTGAGAGGGAAACGAGACTCCA‑AATAGCGACCACGACCTAGCCTAGGTGGTGCTCTACCAACTGAGCTATTCCGGCGTAGTAGTTAATTTTGGtt                                  >  6:1261899/1‑151 (MQ=9)
                                                                                                                   ccAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTCCCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCttt                                 >  7:848856/1‑151 (MQ=255)
                                                                                                                   caaaCTGAGCTATTCGAGCTTTTGTGGTCTGTGCTTTGCATTACTTTTCAATTTTTGCTTCGGTTATCGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCttt                                 <  4:1349449/149‑1 (MQ=17)
                                                                                                                     aaCTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCg                               >  1:873175/1‑151 (MQ=255)
                                                                                                                     aaCTGAGCTATTCCCGCTTGGGTGGTATGTTGTGTTCAGCACTTTTCAAATTTTTGAGGGGTAAACGAGCCACGA‑AATAGCGACCACTCGCTGGGGAAGGAAGGGCGCTACAAACTGATATATTCCCGATTACTACTAAATATTGAtttat                               >  5:951286/1‑149 (MQ=9)
                                                                                                                      aCTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGCAACGAGACTCGA‑ACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTGCCAACTGAGCTATTCACGCGTACTACTTAATTTTGCTTTCGt                              >  7:750664/1‑151 (MQ=255)
                                                                                                                      aCTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGt                              >  2:1216354/1‑151 (MQ=255)
                                                                                                                       cTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGt                              >  3:226462/1‑150 (MQ=255)
                                                                                                                        tGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGA‑ACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTa                             >  6:381670/1‑150 (MQ=255)
                                                                                                                          aGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAtt                          >  1:1797896/1‑151 (MQ=255)
                                                                                                                             tATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTc                       >  2:1373317/1‑151 (MQ=255)
                                                                                                                                tCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     >  6:91199/1‑150 (MQ=255)
                                                                                                                                tCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     >  4:275383/1‑150 (MQ=255)
                                                                                                                                   cGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAttt                 >  4:1709447/1‑151 (MQ=255)
                                                                                                                                   cGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAttt                 >  4:1107761/1‑151 (MQ=255)
                                                                                                                                    gCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAGACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTc                >  1:225638/1‑151 (MQ=255)
                                                                                                                                    gCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACCCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTc                >  7:140140/1‑151 (MQ=255)
                                                                                                                                      ttGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTg              >  1:10125/1‑151 (MQ=255)
                                                                                                                                      gttggggggcgtggCTTTGCCGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTg              <  5:1168553/138‑1 (MQ=255)
                                                                                                                                       tGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACACAGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAAATTTGCTTTAGTAATTTTCGAATTTCTGc             >  6:1511777/1‑151 (MQ=255)
                                                                                                                                            ggtCAGTGCTTTTCATATCTTTTCAAATATTGGAGACGGAAACGAGACTGGA‑AAAGGCTTCGCCGACCTTTGCTATTGCGTTCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtcgt        <  1:725017/151‑1 (MQ=19)
                                                                                                                                                tgtgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACg    >  5:811211/1‑151 (MQ=255)
                                                                                                                                                 gtgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACgg   >  2:515022/1‑151 (MQ=255)
                                                                                                                                                 gtgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACgg   <  3:1702419/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGA‑ACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121976‑4122272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑