Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 482,807 A→T D44V (GAT→GTT)  mreB → rod shape‑determining protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1482,8070AT94.5% 1496.3 / 47.1 476D44V (GAT→GTT) mreBrod shape‑determining protein
Reads supporting (aligned to +/- strand):  ref base A (16/10);  new base T (225/225);  total (241/235)
Fisher's exact test for biased strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

Only 100 of 476 total aligned reads displayed.

TCAGGATTATCCCTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGATCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGAAAAAATGCTCCAGCA  >  DH1/482659‑482954
                                                                                                                                                    |                                                                                                                                                   
tCAGGATTATCCCTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTc                                                                                                                                                   >  4:231621/1‑151 (MQ=255)
 cAGGATTATCCCTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTc                                                                                                                                                   <  5:40262/150‑1 (MQ=255)
  atgATTATCCCTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGt                                                                                                                                                 <  4:1563009/149‑1 (MQ=255)
  aGGATTATCCCTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGt                                                                                                                                                 <  8:1301064/151‑1 (MQ=255)
    gATTATCCCTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGATCGTGc                                                                                                                                               >  5:865718/1‑151 (MQ=255)
     aTTATCCCTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGcc                                                                                                                                              >  8:600826/1‑151 (MQ=255)
     aTTATCCCTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGcc                                                                                                                                              >  1:1311436/1‑151 (MQ=255)
     aTTATCCCTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATAAACTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTACGTGGTGGCCATTCGTCAGGTTCGTGc                                                                                                                                               >  8:876167/1‑150 (MQ=255)
      ttATCCCTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCg                                                                                                                                             >  3:428737/1‑151 (MQ=255)
          cccTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTc                                                                                                                                         >  6:834877/1‑151 (MQ=255)
          cccTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTc                                                                                                                                         >  5:1533165/1‑151 (MQ=255)
             ttAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCAcc                                                                                                                                      >  5:986468/1‑151 (MQ=255)
              tAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCCCCg                                                                                                                                     >  3:1722773/1‑151 (MQ=255)
                 tATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCGGGTTCACCGaa                                                                                                                                   >  3:195742/1‑150 (MQ=255)
                    gTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCAACGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGc                                                                                                                               <  7:1588628/151‑1 (MQ=255)
                     ttGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCg                                                                                                                              >  2:591325/1‑151 (MQ=255)
                      tGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGt                                                                                                                             >  3:85830/1‑151 (MQ=255)
                       gAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGt                                                                                                                             <  8:471889/150‑1 (MQ=255)
                        aaaaaaTTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGt                                                                                                                             >  6:31058/1‑149 (MQ=255)
                          aaaaTTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCt                                                                                                                         <  2:344825/151‑1 (MQ=255)
                           aaaTTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTg                                                                                                                        >  6:69736/1‑151 (MQ=255)
                             aTTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCa                                                                                                                      <  5:1045475/151‑1 (MQ=255)
                               ttCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAg                                                                                                                     <  8:1396863/150‑1 (MQ=255)
                               ttCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGt                                                                                                                    >  1:1083488/1‑151 (MQ=255)
                                 cGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAg                                                                                                                  <  5:1143582/151‑1 (MQ=255)
                                    ggCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGt                                                                                                                <  3:139626/150‑1 (MQ=255)
                                    ggCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTc                                                                                                               <  7:296642/151‑1 (MQ=255)
                                         gTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGAc                                                                                                          <  7:1544041/151‑1 (MQ=255)
                                         gTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGAc                                                                                                          >  7:1787363/1‑151 (MQ=255)
                                            ttCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGAcgc                                                                                                        <  1:1449762/150‑1 (MQ=255)
                                             tCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGAcgcg                                                                                                       >  2:1016012/1‑150 (MQ=255)
                                              ccAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGa                                                                                                      <  2:1199355/150‑1 (MQ=255)
                                                  tGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATCTAAAAGGACAAGGCATCCTATTCAATGAGCCTTCCGTGGTGGCCATTCGTCAGGATCGTGCCGGTTCACCGAAAAGCGTAGCTCCAGTAGGACATGACGCGAAGc                                                                                                   >  4:1204122/1‑149 (MQ=255)
                                                      ttGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGc                                                                                             >  2:787179/1‑151 (MQ=255)
                                                           cATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGc                                                                                        >  5:1649788/1‑151 (MQ=255)
                                                               gACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTa                                                                                    <  3:1806751/151‑1 (MQ=255)
                                                                 ccTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCTGATGCTGGGCCGTagg                                                                                  >  2:84523/1‑149 (MQ=255)
                                                                   tGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGcc                                                                                >  3:528596/1‑151 (MQ=255)
                                                                   tGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGcc                                                                                >  4:1059477/1‑151 (MQ=255)
                                                                   tGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGcc                                                                                >  5:54372/1‑151 (MQ=255)
                                                                       tACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGc                                                                            >  1:83071/1‑151 (MQ=255)
                                                                        aCTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCa                                                                           <  2:1346337/151‑1 (MQ=255)
                                                                          tGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAat                                                                         >  3:567013/1‑151 (MQ=255)
                                                                          tGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAat                                                                         >  6:1417744/1‑151 (MQ=255)
                                                                          tGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAat                                                                         >  2:1792603/1‑151 (MQ=255)
                                                                          tGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGATCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAat                                                                         >  8:109430/1‑151 (MQ=255)
                                                                           gCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAat                                                                         <  5:1302287/150‑1 (MQ=255)
                                                                             gAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATAtt                                                                      <  3:333952/151‑1 (MQ=255)
                                                                               aTACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATtgc                                                                    >  4:1749871/1‑151 (MQ=255)
                                                                                  cccTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGATCGTGCCGGTTCACCGAAAAGCGTATCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATtgctgc                                                                 <  3:96920/151‑1 (MQ=255)
                                                                                       aTTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTc                                                            <  7:249053/151‑1 (MQ=255)
                                                                                        tttATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAAGATTGCTGCCATTCg                                                           >  2:52054/1‑151 (MQ=255)
                                                                                         ttATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGCCGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGc                                                          >  6:442342/1‑151 (MQ=255)
                                                                                         ttATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGc                                                          >  8:296324/1‑151 (MQ=255)
                                                                                             gTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCaa                                                      <  5:69863/151‑1 (MQ=255)
                                                                                             gTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCa                                                       >  5:344452/1‑150 (MQ=255)
                                                                                             gTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGGGGCCATTCGTCAGGTGCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCa                                                       <  6:1220169/150‑1 (MQ=255)
                                                                                               aaaaGGACAAGGCATCGTATTGACTGAGCCTTCCGGGGGGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATg                                                    <  8:1579789/151‑1 (MQ=255)
                                                                                               aaaaGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATg                                                    <  6:1037674/151‑1 (MQ=255)
                                                                                               aaaaGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATg                                                    <  7:429418/151‑1 (MQ=255)
                                                                                                 aaGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGa                                                   >  5:1372064/1‑150 (MQ=255)
                                                                                                   ggACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTAACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGaa                                                  >  7:851698/1‑149 (MQ=255)
                                                                                                    gACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGaa                                                  >  3:913598/1‑148 (MQ=255)
                                                                                                       aaGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACgg                                            >  7:1086056/1‑151 (MQ=255)
                                                                                                       aaGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACgg                                            <  5:325508/151‑1 (MQ=255)
                                                                                                       aaGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACgg                                            <  4:960756/151‑1 (MQ=255)
                                                                                                           cATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGtt                                        >  6:178792/1‑151 (MQ=255)
                                                                                                              cGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATc                                     <  1:541403/151‑1 (MQ=255)
                                                                                                              cGTATTGAATGAGCCTTCCGTGGTGGCCATTCGGCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATc                                     <  1:1593744/151‑1 (MQ=255)
                                                                                                               gTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGAGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCg                                    <  2:1634329/151‑1 (MQ=255)
                                                                                                                   tGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCg                                 <  4:719034/150‑1 (MQ=255)
                                                                                                                   tGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCg                                 <  2:990839/150‑1 (MQ=255)
                                                                                                                     aaTGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGAct                              >  6:1649424/1‑151 (MQ=255)
                                                                                                                      aTGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGActt                             >  7:725732/1‑151 (MQ=255)
                                                                                                                      aTGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCCGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGActt                             >  8:1657860/1‑151 (MQ=255)
                                                                                                                       tGAGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTATCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGActtc                            >  5:1206184/1‑151 (MQ=255)
                                                                                                                         aGCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGActtctt                          <  3:1631851/151‑1 (MQ=255)
                                                                                                                          gCCTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGGCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACttcttc                         <  8:1713084/151‑1 (MQ=255)
                                                                                                                          cccTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACttcttc                         <  3:1219683/150‑1 (MQ=255)
                                                                                                                           ccTTCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCg                        >  3:807811/1‑151 (MQ=255)
                                                                                                                              tCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTg                      >  1:1756481/1‑150 (MQ=255)
                                                                                                                              tCCGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCAATCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTg                      <  6:1150230/150‑1 (MQ=255)
                                                                                                                              tCCGTGGTGGCCATTCGTCAGGTGCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCTAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGt                       <  4:1267752/149‑1 (MQ=255)
                                                                                                                                cGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACt                   >  6:1004161/1‑151 (MQ=255)
                                                                                                                                cGTGGTGGCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGAAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCCGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCAGACTTCTTCGTGACt                   >  4:31170/1‑151 (MQ=255)
                                                                                                                                cGTGGTGGCCATTCGTCAGGATCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACt                   >  7:597106/1‑151 (MQ=255)
                                                                                                                                  tggtggCCATTCGTCAGGATCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTg                  >  1:684495/1‑150 (MQ=255)
                                                                                                                                   ggtggCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGaa                <  1:327408/151‑1 (MQ=255)
                                                                                                                                   ggtggCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGa                 <  6:592010/150‑1 (MQ=255)
                                                                                                                                   ggtggCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGa                 <  7:1433417/150‑1 (MQ=255)
                                                                                                                                   ggtggCCATTCGGCAGGTTCGTGCCGGTGCACCTAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGa                 <  4:625709/150‑1 (MQ=255)
                                                                                                                                     tggCCATTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGaaa               >  4:1415579/1‑150 (MQ=255)
                                                                                                                                          aTTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGAAAAAAtc          >  7:1702317/1‑149 (MQ=255)
                                                                                                                                          aTTCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGAAAAAATGc         >  1:1250514/1‑151 (MQ=255)
                                                                                                                                           ttCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGACCGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGGTATCGCCCCCTTCTTCGTGACTGAAAAAATGCt        >  1:1002739/1‑151 (MQ=255)
                                                                                                                                            tCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGAAAAAATGCt        <  5:1784667/150‑1 (MQ=255)
                                                                                                                                            tCGTCAGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGAAAAAATGCTc       >  7:1323374/1‑151 (MQ=255)
                                                                                                                                              gtgaGGTTCGTGCCGGTGCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGAGGCTGGGCGGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGAAAAAATGCTCCa     <  8:1033990/148‑1 (MQ=255)
                                                                                                                                              gTCAGGTTCGTGCCGGTGCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGAAAAAATGCTCCa     <  6:270775/151‑1 (MQ=255)
                                                                                                                                                 aGGTTCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGAAAAAATGCTCCAGCa  <  2:138985/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCAGGATTATCCCTTAGTATGTTGAAAAAATTTCGTGGCATGTTTTCCAATGACTTGTCCATTGACCTGGGTACTGCGAATACCCTCATTTATGTAAAAGGACAAGGCATCGTATTGAATGAGCCTTCCGTGGTGGCCATTCGTCAGGATCGTGCCGGTTCACCGAAAAGCGTAGCTGCAGTAGGTCATGACGCGAAGCAGATGCTGGGCCGTACGCCGGGCAATATTGCTGCCATTCGCCCAATGAAAGACGGCGTTATCGCCGACTTCTTCGTGACTGAAAAAATGCTCCAGCA  >  DH1/482659‑482954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑