Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 635,638 A→C F118L (TTT→TTG)  yqjA ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1635,6380AC96.2% 1753.8 / 33.2 470F118L (TTT→TTG) yqjAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (12/6);  new base C (223/228);  total (235/235)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01

Only 100 of 485 total aligned reads displayed.

AGGATCAATACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGAAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCGTTTGC  >  DH1/635489‑635785
                                                                                                                                                     |                                                                                                                                                   
  gATCAATACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGcaaa                                                                                                                                                  <  6:665674/151‑1 (MQ=255)
     cAATACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAg                                                                                                                                                <  6:269486/150‑1 (MQ=255)
      aaTACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGAt                                                                                                                                              <  5:859168/151‑1 (MQ=255)
         aCCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGgt                                                                                                                                           >  3:276573/1‑151 (MQ=255)
         aCCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGgt                                                                                                                                           <  4:928766/151‑1 (MQ=255)
            cacaGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGCCCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGtgtg                                                                                                                                        >  1:1487932/1‑151 (MQ=255)
            cacaGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGtgtg                                                                                                                                        >  7:767630/1‑151 (MQ=255)
            cacaGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGtgtg                                                                                                                                        >  7:1022648/1‑151 (MQ=255)
               agcagACCGCTAATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTgcgc                                                                                                                                     <  1:71357/151‑1 (MQ=255)
                gcagACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGcgcg                                                                                                                                    >  4:338363/1‑151 (MQ=255)
                 cagACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGt                                                                                                                                   >  7:974612/1‑151 (MQ=255)
                  cgACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATGCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGtt                                                                                                                                  <  2:37320/150‑1 (MQ=255)
                  agACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGtt                                                                                                                                  >  2:932241/1‑151 (MQ=255)
                     ccGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTtgag                                                                                                                               <  4:445801/151‑2 (MQ=255)
                       gCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTtgatga                                                                                                                             <  2:1234772/151‑1 (MQ=255)
                       gCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTtgatg                                                                                                                              >  3:1489471/1‑150 (MQ=255)
                         tCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTgc                                                                                                                                       >  5:406451/1‑139 (MQ=255)
                          cATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTgatgat                                                                                                                            <  7:1249259/149‑1 (MQ=255)
                          cATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATaa                                                                                                                          <  1:376890/151‑1 (MQ=255)
                          cATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATaa                                                                                                                          <  5:1069714/151‑1 (MQ=255)
                            tCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATg                                                                                                                        >  1:324367/1‑151 (MQ=255)
                             ccAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACATCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATgc                                                                                                                       >  1:1155099/1‑151 (MQ=255)
                              cAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATgcg                                                                                                                      <  7:1786965/151‑1 (MQ=255)
                               aGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATgcgc                                                                                                                     <  6:1672228/151‑1 (MQ=255)
                                gTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGcgcg                                                                                                                    <  1:473382/151‑1 (MQ=255)
                                    aaaaaCTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGt                                                                                                                 >  8:1184588/1‑150 (MQ=255)
                                      aaaCTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGAAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTaaa                                                                                                              <  5:1738927/151‑1 (MQ=255)
                                      aaaCTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGGTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTaa                                                                                                               >  4:1644351/1‑150 (MQ=255)
                                       aaCTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAAt                                                                                                             <  7:692066/151‑1 (MQ=255)
                                        aCTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATg                                                                                                            >  1:964773/1‑151 (MQ=255)
                                        aCTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATg                                                                                                            <  3:406468/151‑1 (MQ=255)
                                          tGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATg                                                                                                            >  7:486270/1‑149 (MQ=255)
                                           gAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATga                                                                                                           <  2:11051/149‑1 (MQ=255)
                                             aaGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGAt                                                                                                        >  5:295111/1‑150 (MQ=255)
                                               gcgcgcGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGc                                                                                                     >  7:1459912/1‑151 (MQ=255)
                                               ccgcgcgGTATTCAGCCCCGCTAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGGGTGCGCGTTGATGCTAATGCGCGGGTAAATGAGATAGc                                                                                                     <  2:185774/150‑1 (MQ=255)
                                                cgcgcgTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGcc                                                                                                    <  8:18534/151‑1 (MQ=255)
                                                cgcgcgTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGcc                                                                                                    >  1:1107887/1‑151 (MQ=255)
                                                  cgcgTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGAAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGcc                                                                                                    >  3:1688923/1‑149 (MQ=255)
                                                  cgcgTTATTCAGCCCTGATAACCCGGCAATCGGCGGCAGCAGTGGTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAg                                                                                                  <  8:1648709/151‑1 (MQ=255)
                                                   gcgTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGt                                                                                                 >  7:54309/1‑151 (MQ=255)
                                                    cgTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGGGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGtt                                                                                                >  8:131780/1‑151 (MQ=255)
                                                     gTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGttt                                                                                               >  7:158164/1‑151 (MQ=255)
                                                      ttATTCAGCCCTGATAACCCGGCAGTCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGtttt                                                                                              >  5:1795830/1‑151 (MQ=255)
                                                      ttATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGttt                                                                                               <  7:1632475/150‑1 (MQ=255)
                                                       tATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTg                                                                                             >  2:583331/1‑151 (MQ=255)
                                                       tATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTg                                                                                             >  8:673495/1‑151 (MQ=255)
                                                        aTTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTg                                                                                             <  2:1700821/150‑1 (MQ=255)
                                                         ttCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGt                                                                                            >  1:1265193/1‑150 (MQ=255)
                                                         ttCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGt                                                                                            >  1:897647/1‑150 (MQ=255)
                                                           cAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGAGCGGGTAAATGAGATAGCCAGTTTTGTACg                                                                                         >  3:850688/1‑151 (MQ=255)
                                                              cccTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTg                                                                                      <  3:1050447/151‑1 (MQ=255)
                                                              cccTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTg                                                                                      >  5:1295730/1‑151 (MQ=255)
                                                              cccTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTg                                                                                      >  7:357638/1‑151 (MQ=255)
                                                                 tGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCgg                                                                                   >  1:649308/1‑151 (MQ=255)
                                                                   aTAACCCGGCAATCGGCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAATCCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGt                                                                                 <  6:1265401/151‑1 (MQ=255)
                                                                     aaCCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTa                                                                                <  7:412771/150‑1 (MQ=255)
                                                                         cGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGcc                                                                           >  5:69435/1‑151 (MQ=255)
                                                                               tcgtcgGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCAAGcc                                                                      >  2:1510841/1‑150 (MQ=255)
                                                                                 gtcgGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATc                                                                   <  3:924161/151‑1 (MQ=255)
                                                                                  tcgGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCg                                                                  >  4:1792856/1‑151 (MQ=255)
                                                                                  tcgGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCg                                                                  >  3:655436/1‑151 (MQ=255)
                                                                                  tcgGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCg                                                                  >  6:1574948/1‑151 (MQ=255)
                                                                                      cagcagTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGcccc                                                              >  5:1160423/1‑151 (MQ=255)
                                                                                      cagcagTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGcccc                                                              <  1:100704/151‑1 (MQ=255)
                                                                                       agcagTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCt                                                             >  1:1607986/1‑151 (MQ=255)
                                                                                        gcagTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGCTGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTg                                                            <  1:203525/151‑1 (MQ=255)
                                                                                          agTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTAT‑TCAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGCGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAat                                                         <  7:1521687/151‑1 (MQ=255)
                                                                                             gtTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAatat                                                       <  5:166809/151‑1 (MQ=255)
                                                                                              ttCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAatat                                                       >  8:489985/1‑150 (MQ=255)
                                                                                               tCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAatat                                                       >  2:1661924/1‑149 (MQ=255)
                                                                                                  gACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGc                                                    <  5:808108/149‑1 (MQ=255)
                                                                                                      aaCGCAATATAGCGACCATTTAACAGCGCCGATAAACCGTCTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCACTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGAccc                                              <  1:1811211/151‑1 (MQ=255)
                                                                                                       aCGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGAccc                                              >  5:1466434/1‑150 (MQ=255)
                                                                                                       aCGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCca                                             <  2:1773791/151‑1 (MQ=255)
                                                                                                          cAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCGCAGCCATCGCCCCTGAATATAGCTGACCcagc                                           >  6:1078/1‑150 (MQ=255)
                                                                                                           aaTAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCcagc                                           >  4:622824/1‑149 (MQ=255)
                                                                                                            aTAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCagcagc                                        <  2:730786/151‑1 (MQ=255)
                                                                                                              aaaGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGcc                                       <  8:243095/150‑1 (MQ=255)
                                                                                                              aaaGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGcc                                       <  6:1444335/150‑1 (MQ=255)
                                                                                                              aaaGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCg                                      <  8:330616/151‑1 (MQ=255)
                                                                                                                aGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAg                                    <  4:798725/151‑1 (MQ=255)
                                                                                                                 gCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGGGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAgg                                   <  3:119974/151‑1 (MQ=255)
                                                                                                                  cGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGc                                  >  7:1850706/1‑151 (MQ=255)
                                                                                                                        aTTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTggcgg                            >  2:81897/1‑151 (MQ=255)
                                                                                                                        aTTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTggcgg                            >  4:1332225/1‑151 (MQ=255)
                                                                                                                        aTTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTggcgg                            >  6:503310/1‑151 (MQ=255)
                                                                                                                             cAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACg                       <  6:1795670/151‑1 (MQ=255)
                                                                                                                              aGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACgg                      >  7:862056/1‑151 (MQ=255)
                                                                                                                                  ccGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGGcag                  <  2:1379432/151‑1 (MQ=255)
                                                                                                                                   cGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTcagc                 >  8:1229926/1‑151 (MQ=255)
                                                                                                                                    caTAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTcagca                <  6:800398/150‑1 (MQ=255)
                                                                                                                                      tAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTcagcagc              >  6:947751/1‑151 (MQ=255)
                                                                                                                                      tAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGAACCAGCAGCCGAGGCTGGCGGCAACGGTcagcagc              >  8:1718117/1‑151 (MQ=255)
                                                                                                                                            gtgtTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATc        >  4:218377/1‑151 (MQ=255)
                                                                                                                                             tgtTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGAACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGGCAGCAGCAGAATCg       >  2:512880/1‑151 (MQ=255)
                                                                                                                                              gtTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCg       <  1:1680307/150‑1 (MQ=255)
                                                                                                                                              gtTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCg       <  2:1438784/150‑1 (MQ=255)
                                                                                                                                                  aTGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCGTTTGc  >  4:1190743/1‑151 (MQ=255)
                                                                                                                                                  aTGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCGTTTGc  <  4:1099902/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AGGATCAATACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGAAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCGTTTGC  >  DH1/635489‑635785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑