Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,571,230 C→A R67S (CGC→AGC)  cls → cardiolipin synthase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,571,2300CA96.0% 1565.4 / 22.6 449R67S (CGC→AGC) clscardiolipin synthase A
Reads supporting (aligned to +/- strand):  ref base C (13/5);  new base A (219/212);  total (232/217)
Fisher's exact test for biased strand distribution p-value = 9.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 453 total aligned reads displayed.

GTTGCTCATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATCGCTGGGG  >  DH1/2571082‑2571377
                                                                                                                                                    |                                                                                                                                                   
  tGCTCATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCagcg                                                                                                                                                  <  3:445783/150‑1 (MQ=255)
   gCTCATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCt                                                                                                                                                >  3:223004/1‑151 (MQ=255)
   gCTCATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGGTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCt                                                                                                                                                <  6:1118608/151‑1 (MQ=255)
      cATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAg                                                                                                                                             <  4:1004761/151‑1 (MQ=255)
        ttGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAgcg                                                                                                                                           >  2:156632/1‑151 (MQ=255)
             ggCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCAGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCa                                                                                                                                      <  2:930733/151‑1 (MQ=255)
              gCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGcc                                                                                                                                       >  1:1335192/1‑149 (MQ=255)
              gCGTAACTTTACGCATGCTACTGAAACGACGCGCAGGTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCag                                                                                                                                     <  2:357293/151‑1 (MQ=255)
                 tAACTTTACGCATTCTAATGAAACGACGCGCGGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGc                                                                                                                                  >  5:441238/1‑151 (MQ=255)
                 tAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGc                                                                                                                                  >  7:1273262/1‑151 (MQ=255)
                  aaCTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGc                                                                                                                                  <  6:1406879/150‑1 (MQ=255)
                     tttACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGAtg                                                                                                                              >  4:1489759/1‑151 (MQ=255)
                      ttACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGCGCTCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGAtgt                                                                                                                             >  4:747983/1‑151 (MQ=255)
                         cGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGc                                                                                                                          >  3:828249/1‑151 (MQ=255)
                              tCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTcc                                                                                                                     >  5:1211612/1‑151 (MQ=255)
                               cTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTccc                                                                                                                    >  6:764068/1‑150 (MQ=255)
                                  aTGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCg                                                                                                                 >  5:926267/1‑151 (MQ=255)
                                     aaaCGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCa                                                                                                               >  7:1062329/1‑150 (MQ=255)
                                      aaCGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGGCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCaaa                                                                                                             <  2:221188/151‑1 (MQ=255)
                                        cgacgCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGATGAGCGCGCCAGAGCGATGTGGCCTGCCCCCGCAAAAt                                                                                                           >  4:1664727/1‑151 (MQ=255)
                                         gacgCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATg                                                                                                          >  7:274599/1‑151 (MQ=255)
                                         gacgCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATg                                                                                                          >  7:116344/1‑151 (MQ=255)
                                           cgcgCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGc                                                                                                        >  5:1006317/1‑151 (MQ=255)
                                             cgcAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCtt                                                                                                      <  4:327914/151‑1 (MQ=255)
                                              ggaGTTCCCTCCGCGATGGCCTGGCTGTTGATTA‑TTACAATAATCCGTTACTAGGAATTAATTCCAATCATTCCGTTTGGGAACAACATTTACGCAAACCCCCCGCTGACGGCGCCAGAGCGCTGTGGCCTTACACCGCAAAATGGCTTa                                                                                                     >  1:45346/3‑150 (MQ=255)
                                              gcAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCtt                                                                                                      >  1:636647/1‑150 (MQ=255)
                                                aGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCCTAAc                                                                                                   <  2:849840/151‑1 (MQ=255)
                                                 gTTCGCTCCGCGATGGCCTGGCTGCTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTCGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACAGCAAAATGGCTTAACg                                                                                                  <  6:1701653/151‑1 (MQ=255)
                                                     ccTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCt                                                                                              >  1:909486/1‑151 (MQ=255)
                                                        ccGCGATGGCGTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCtt                                                                                             <  2:1700588/149‑1 (MQ=255)
                                                        ccGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTa                                                                                            >  1:1654425/1‑150 (MQ=255)
                                                         cgcgATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTaaa                                                                                          <  6:334821/151‑1 (MQ=255)
                                                           cgATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGc                                                                                        >  1:533688/1‑151 (MQ=255)
                                                             aTGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCt                                                                                      <  3:1521262/151‑1 (MQ=255)
                                                                 ccTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGGCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTaa                                                                                  <  2:1374946/151‑1 (MQ=255)
                                                                 ccTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTaa                                                                                  <  3:583468/151‑1 (MQ=255)
                                                                   tGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGc                                                                                >  5:1786383/1‑151 (MQ=255)
                                                                     gCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCAGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCat                                                                              >  4:1298624/1‑151 (MQ=255)
                                                                      cagTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCata                                                                             <  7:1840070/149‑1 (MQ=255)
                                                                       tggtGATTATTTACATTCTGCCGTTAGGCGGAATTATTGGCTATCTTGCCGTTGGCGAGCTCCCGTTAGGCAAACGCAGCCCTGAGCGCGCCAGAGCGATGTGGCCTGGCACCGCAAAATGGCTTATCGACCTTAATGCCTGTAAGCatat                                                                            >  8:1011972/4‑151 (MQ=255)
                                                                       tGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCatat                                                                            >  2:1461242/1‑151 (MQ=255)
                                                                          tGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCtt                                                                         >  5:1599969/1‑151 (MQ=255)
                                                                                 ttACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCga                                                                   >  3:290865/1‑150 (MQ=255)
                                                                                 ttACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCga                                                                   >  3:387453/1‑150 (MQ=255)
                                                                                  tACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCgatg                                                                 >  6:288579/1‑149 (MQ=255)
                                                                                     aTTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCgaagaa                                                               >  3:1267963/1‑150 (MQ=255)
                                                                                       tCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAAt                                                            >  4:503349/1‑151 (MQ=255)
                                                                                       tCTGCCGTTAGGCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGaaaa                                                             <  8:24484/150‑1 (MQ=255)
                                                                                          gCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATa                                                           <  7:1753360/149‑1 (MQ=255)
                                                                                          gCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGc                                                         <  6:1814411/151‑1 (MQ=255)
                                                                                          gCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGc                                                         >  1:1562533/1‑151 (MQ=255)
                                                                                          gCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGc                                                         >  5:1113890/1‑151 (MQ=255)
                                                                                          gCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGc                                                         >  3:1424342/1‑151 (MQ=255)
                                                                                           ccGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGaaaagtgc                                                         >  6:921666/1‑146 (MQ=255)
                                                                                            cGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGc                                                         >  1:239796/1‑149 (MQ=255)
                                                                                             gTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAgt                                                      <  8:1134390/151‑1 (MQ=255)
                                                                                             gTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAgt                                                      <  1:1478688/151‑1 (MQ=255)
                                                                                              ttAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAgtg                                                     >  5:1708869/1‑151 (MQ=255)
                                                                                               tAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAgtg                                                     <  5:646218/150‑1 (MQ=255)
                                                                                                aGTCGGAATTATTGCCTATCTTGCCGGTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAgtgt                                                    <  8:220522/150‑1 (MQ=255)
                                                                                                 gTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCg                                                  <  1:346187/151‑1 (MQ=255)
                                                                                                  tCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGc                                                 >  7:744474/1‑151 (MQ=255)
                                                                                                   cGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCt                                                <  5:1456118/151‑1 (MQ=255)
                                                                                                    ggAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTg                                               <  6:1474716/151‑1 (MQ=255)
                                                                                                    ggAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTg                                               >  4:1752309/1‑151 (MQ=255)
                                                                                                    ggAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTg                                               <  1:1077679/151‑1 (MQ=255)
                                                                                                       attattGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTgcgc                                            >  8:1329522/1‑151 (MQ=255)
                                                                                                          attGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCat                                         <  8:1502775/151‑1 (MQ=255)
                                                                                                            tGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCatt                                        <  6:1521855/150‑1 (MQ=255)
                                                                                                             gCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCattat                                      >  6:457619/1‑151 (MQ=255)
                                                                                                              ccTATCTCGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCattatt                                     <  1:72420/151‑1 (MQ=255)
                                                                                                                   cTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGc                                >  4:1213313/1‑151 (MQ=255)
                                                                                                                    ttGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCt                               >  7:564174/1‑151 (MQ=255)
                                                                                                                     tGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAg                                 <  3:1055728/148‑1 (MQ=255)
                                                                                                                     tGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGc                                <  2:790180/149‑1 (MQ=255)
                                                                                                                      gCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCttt                             <  6:1262574/151‑1 (MQ=255)
                                                                                                                      gCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCttt                             <  6:1056854/151‑1 (MQ=255)
                                                                                                                       ccGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTg                            >  2:1203908/1‑151 (MQ=255)
                                                                                                                       ccGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTg                            >  2:593008/1‑151 (MQ=255)
                                                                                                                        cGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTAAAGCTTTGc                           >  2:729455/1‑151 (MQ=255)
                                                                                                                        cGTTGGCGAGCTCCAGTTAGGCAAACGCAGCGCTGAGCGCGCCAGCGCGATGTGGCCTTCCCCCGCAAAATGGCTGAACGACCTTAAAGCCTGTAAGCATATCTGCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGc                           >  2:111172/1‑151 (MQ=255)
                                                                                                                         gTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGTAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCg                          >  8:1711546/1‑151 (MQ=255)
                                                                                                                         gTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTgtg                          <  5:331957/151‑3 (MQ=255)
                                                                                                                         gTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCg                          >  5:1341326/1‑151 (MQ=255)
                                                                                                                         attGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCg                          <  2:488806/150‑1 (MQ=255)
                                                                                                                           tGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCg                          >  4:950038/1‑149 (MQ=255)
                                                                                                                            ggCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAg                        <  8:581469/150‑1 (MQ=255)
                                                                                                                            ggCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGc                       <  8:735512/151‑1 (MQ=255)
                                                                                                                            ggCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGc                       <  3:889615/151‑1 (MQ=255)
                                                                                                                             gCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGcg                      <  1:423778/151‑1 (MQ=255)
                                                                                                                              cGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGcgt                     >  8:1305999/1‑151 (MQ=255)
                                                                                                                                   tCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCgtcgtc                 >  7:1403597/1‑150 (MQ=255)
                                                                                                                                   tCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCCCCCCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAAAAGCAGTGACGCAGCGCCATTATTCAAGCAATGAGAGCgtcgtc                 >  4:215313/1‑150 (MQ=255)
                                                                                                                                        ttAGGCAAAGGCAGCGCTGAGCGCTGCAGAGCGTTGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAATGCCTGTAATCATATCTTCGCCGAAGAAAATAGCAGTGGGGCTGGGCGATTATTGAAGCTTTGCGAGCGTCGTCAgggg            <  5:682931/150‑1 (MQ=255)
                                                                                                                                          aGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGAt          <  3:1751674/150‑1 (MQ=255)
                                                                                                                                          aGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGAt          <  8:337606/150‑1 (MQ=255)
                                                                                                                                             cAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATCGCt      <  2:419807/151‑1 (MQ=255)
                                                                                                                                              aaaCGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATCGCTg     <  3:1152126/151‑1 (MQ=255)
                                                                                                                                                aCGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATCGCTggg   >  1:1712869/1‑151 (MQ=255)
                                                                                                                                                aCGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATCGCTggg   <  7:1611337/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTTGCTCATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATCGCTGGGG  >  DH1/2571082‑2571377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑