Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,5890GT22.2% 402.8 / 13.8 223intergenic (+450/+573)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base G (66/92);  new base T (8/37);  total (77/146)
Fisher's exact test for biased strand distribution p-value = 2.87e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

AAGGTACGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACT  >  DH1/613439‑613736
                                                                                                                                                        |                                                                                                                                                   
aaGGTACGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTgg                                                                                                                                                     <  6:901838/151‑1 (MQ=255)
 aGGTACGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGa                                                                                                                                                    <  1:875671/151‑1 (MQ=255)
  ggTACGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTgg                                                                                                                                                     >  8:807026/1‑149 (MQ=255)
    tACGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCTGAGCGTATTTGCTTATGTAggg                                                                                                                                                 >  3:238476/1‑151 (MQ=38)
     aCGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAgggg                                                                                                                                                >  5:997982/1‑151 (MQ=255)
     aCGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAgggg                                                                                                                                                <  2:203948/151‑1 (MQ=255)
      cGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGc                                                                                                                                               <  4:973412/151‑1 (MQ=255)
         cccGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAgggga                                                                                                                                               >  1:697328/1‑147 (MQ=255)
         cccGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                                                                                                                                            <  3:45428/151‑1 (MQ=255)
         cccGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                                                                                                                                            <  6:1125992/151‑1 (MQ=255)
           cGTACTGAACCGGGGTAGGCTGCTTGAGCCAGTGCGCGATTGCTGCCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaaa                                                                                                                                          <  4:1055561/151‑1 (MQ=255)
            gTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGgagaggt                                                                                                                                         >  1:1363925/1‑148 (MQ=255)
             tACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGTTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTACTTTTGGAGGGGAAgtacg                                                                                                                                         >  1:37394/1‑146 (MQ=255)
              aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGat                                                                                                                                       <  2:1474563/151‑1 (MQ=255)
                 gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaa                                                                                                                                    <  7:1444314/151‑1 (MQ=255)
                 gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                                                                                                                                      <  3:1050381/149‑1 (MQ=255)
                 gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTCCTTTTGGAGGTGCAGAAAGatca                                                                                                                                     >  4:519546/1‑148 (MQ=255)
                 gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGAAAAAACA‑AG‑CTACGCCGGGTTTTTCATTTTGTAGGGGCATAAAGatga                                                                                                                                     >  8:545635/1‑150 (MQ=37)
                 gAACCCGGGGAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatga                                                                                                                                     <  4:1369330/150‑1 (MQ=255)
                   aCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg                                                                                                                                  <  3:1241658/151‑1 (MQ=255)
                   aCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg                                                                                                                                  <  5:134941/151‑1 (MQ=255)
                      cGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACt                                                                                                                               <  1:1431531/151‑1 (MQ=255)
                       gggTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTg                                                                                                                              <  5:1095799/151‑1 (MQ=255)
                           aGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCa                                                                                                                          <  4:1152603/151‑1 (MQ=255)
                             gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacg                                                                                                                        <  3:182743/151‑1 (MQ=255)
                              ctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAAACCCATTATCAATCACTTTAACCAGAATTACATAAAAACA‑CT‑CTTCCTCGAGTTTTTGATTTTGGAGGTGCAGAAAGATGAATAACtataaacg                                                                                                                        >  2:535243/1‑143 (MQ=255)
                               tgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAACCCCCTAATCAATCAGATACAAATAATTTACTTAAAAAAA‑AG‑ATTCTGCCTCTTTTTGATTTTGTAGGGGCAGAAAGattaatgaatataaaagac                                                                                                                      >  2:1414187/1‑140 (MQ=255)
                                gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgacg                                                                                                                     <  2:795952/151‑1 (MQ=255)
                                gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCacgac                                                                                                                      <  2:1020381/150‑1 (MQ=255)
                                  ttGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACACGGCTTATCCGTCAGTTTCAGCTGATTAACGTAAAAACC‑CC‑CTTCGGCCCCTTTTTGGTATTGCAGGTGCAGAAAGATGAATGACTGTCCACGACCCt                                                                                                                   >  1:1045228/1‑151 (MQ=21)
                                   tgcgCCAGTGAGCCATTCCTGCCCTAGATGAATGACTGTCCACGACAGAACCGGGCTTAGCGGTCAGTTTCACCTTATTTACTTAAAAACC‑TG‑CTTCGGGGGTTTTTTGTTTTTGTAGGGTCATAAATATTAATTACTGTCCACGACGCt                                                                                                                   <  4:440201/147‑1 (MQ=18)
                                   ggAGCCAGGGAGCCATGGCTGGCCTAGCTGAATGACGGTCCACGACAGAACCCGGCTTATCGGGCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGAGGAATGACTGTCCACGACGCta                                                                                                                  <  2:932509/150‑1 (MQ=18)
                                       ccAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACTCTATAcc                                                                                                              >  7:1501260/1‑151 (MQ=255)
                                       ccAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAAACCGCTAATAAGACAGTTTAAAATGATTTAAGTAAAAAAC‑AA‑CTTCTAAATGTTTTTGCTATTGGATTGACAGAAATATGAATGACTGTACACGACACTATAcc                                                                                                              >  8:1323907/1‑151 (MQ=255)
                                        cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTTATGTAACTAAAAACC‑CG‑AATCGCCTGGTTTTTGCTATTTGAGTGGCTCAAAGATGACTGCCTGTCCTAGACACtaacac                                                                                                              >  6:822029/1‑146 (MQ=19)
                                        cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATAGGTCAGTTTAACCTGATTTACGTAAAAACC‑AG‑CTTAGGCGGGTGTTTGGTTTTGGAGGGGCAGATAGATGAATGACTGTCCACGACGCTATAccc                                                                                                             >  5:236888/1‑151 (MQ=21)
                                            gAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAAACCCCAAATCAATCAGATTCAAATGAATTACGTAAAAACA‑AG‑CTTCTGCCGCTTTTTGATTTTGTAGGTGCAGAAAGATGAATTAATATAAAAGACACTATACCCaaa                                                                                                          >  2:822779/1‑150 (MQ=12)
                                               cGATGGCTGGCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTAGCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAaaagaa                                                                                                      <  4:197084/151‑1 (MQ=255)
                                                gATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAaaagaa                                                                                                      >  7:85753/1‑150 (MQ=255)
                                                 aTTGCTGGCGTAGATCAATGACTGTCCCCCACAGACCCCGGCTTAGCGGCCTGTTTCACCTGATTTACGTAAAACCC‑GG‑CTTCGGGGGGTTTTTGGTTTTGGAGGGGCAGAAAGATCAATGACTGGCCACGACGCTATACCCAaaagaaa                                                                                                     <  7:1303324/150‑1 (MQ=255)
                                                       ggCCTATATGAATGCCTGGCCACCACATAACCCGGCTTAGGGGTCAGTTTCACCTTATTTCCGTAAAACCC‑CG‑CTTCGGCGGTTTTTTGGTTTTGTAGGGGCACAAAGATGAATGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                              <  7:93206/151‑1 (MQ=14)
                                                       ggCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACTTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCt                                                                                               <  7:484432/150‑1 (MQ=255)
                                                        gCCTAGATGAATGACTGCCCACGACAGAACCCGGGTTAGCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGGTTTTGGAGGGGCAGAAAGATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTa                                                                                             <  7:259232/151‑1 (MQ=255)
                                                         ccTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGTTTTTTGCTTTTGTCGGGGCACAAATATCAATGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTAt                                                                                            <  8:206981/151‑1 (MQ=18)
                                                            ggacaactccctagccaccaCAGAACGCGGTTTATCGGTCAGTTTCACCTTTTTTACTTAAAAACC‑GG‑CTTCGGCGGGTTTTTGGTTTTGCTGGGGCCCAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCg                                                                                          <  1:850937/136‑1 (MQ=11)
                                                            aGATGAATGACTGTCCCCGACACAACCCGGCTTAGGGGTCAGTTTCACCTGATTTACGTAAAACCC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCgg                                                                                         <  5:1256710/151‑1 (MQ=255)
                                                            aGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCATAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCgg                                                                                         <  6:647398/151‑1 (MQ=255)
                                                             tattaattagtGTCCCCGACAGAACCCGGCTTTTCGTTCAGTTTCACATGATTTACTTAAAAACC‑GG‑CTGCTGGGGTTTTTTGGTTTTGTAGGGGAATAAAGTTTACGACCTGTCCACGACGGTATACCCAAAAGAAAGCGGCTTATCgg                                                                                         <  7:458102/140‑1 (MQ=9)
                                                             gATTAATGACTGACGACTACAGACCCCTGTTTATCTTTCAGTTTAACCTGATTTACGTAAAAACC‑CT‑CTTGTGCGGGTTTTTGCTTTTGTAGGGGCTTAAACATTATTGACTGTCCACTACGCTATACCCAAAAGAAACCGGCTTATCgg                                                                                         <  8:1013224/150‑1 (MQ=255)
                                                             gATTAATGACTGACCACGACAGAACCCGCGTTATGGGTCTGTTTCACCTGATTTACTTAAAAACC‑CG‑CTGGGGGGGTTTTTTGGTTTTGGTGGGGCACAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                        <  5:500809/151‑1 (MQ=17)
                                                              atgaatgaCTTACCACGACAGAACCCGTCTTATCGGTATGTTTCTCCTGATTTACTTAAAAACC‑CG‑CTGCGGCGGGTTTTTTTTTTTGGTGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                        <  1:1068886/150‑1 (MQ=17)
                                                              atcaatgaCTGTCCACGCCACAACCCGGCTTTTCGGTCAGTTTCACGTCATTTACGTAAAAACC‑CG‑CTTGGGCGGGTTTTTGCTTTTGGCGGGGCACAAACTTCAATCACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                        <  3:847212/147‑1 (MQ=14)
                                                                  atgaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACCCTATACCCAaaggaaaaccggagaggggtc                                                                                       >  4:1532065/1‑133 (MQ=17)
                                                                   ggccgccccACAACACAACCCGGCTTAGGGGGAAGTTTCCCCTGATTTCCTTAAACACC‑CG‑CTGCGGCGGTTTTTTGGTTTTGGCGGGGAACAACTATAAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                                                                  <  7:633902/144‑1 (MQ=9)
                                                                    gaCTCTCCACTACAGAACCCGGCTTATCGGTAATTTTCACCTGATTTACTTAAAAACC‑CG‑CTTCGGCGGTTTTTTGTTTTTGGTGGGGCATAAATATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                                                                  <  6:1057421/150‑1 (MQ=14)
                                                                    caCTGGCCACCACAGAACCCGGCTAATCGGCCAGTTTCCCCTGATTTACTTAAAACCC‑CG‑CTGCGGCGGGTTTTTGCTTTTGCAGGGGCACAAACATCAAGGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTc                                                                                 <  7:123239/150‑1 (MQ=14)
                                                                     acatccccccaCAGAACCCGGCTTATGGGCCATTTGCCCCTGATTTCGTTAAAAACC‑GC‑CTGCGCCGGGTTTTTGTTTTTGCTGGGGCATAAACATGAATGACTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTc                                                                                 <  2:464710/140‑1 (MQ=9)
                                                                       tGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAATATGAAGGCCTGTGCACTCCGCTCTACACATACGAAAGCGGCTTATCGGTCAGgttgtc                                                                               >  5:960228/1‑147 (MQ=14)
                                                                        cgccAGGACAGAACCCGGCTTAGCGGCCAGTTGCACTTG‑TTTTCGTTAAAACCGCG‑CTTCGGCGGGTTTTTGCTTTTGTTGGCGCTCAAATATGATGGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCt                                                                             <  6:286567/149‑1 (MQ=9)
                                                                          ccaccaCACAACCCGGCTTATCGGTAAGTTTCAGGTGATTTACTTAAAAACC‑GT‑CTTCTGCGGGTTTTTGTTTTTGTTGGTGCTTAACTATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTgg                                                                           <  2:232129/151‑1 (MQ=14)
                                                                             ggACAGAACCCGGCTTATCGGGCAGTTTCACCTGATTTACGTAAAAACC‑GT‑CTGCGGCGGGTTTTTGCTTTTGGTGGCGCTTAAAGATGACTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt                                                                        <  3:564074/150‑1 (MQ=14)
                                                                              aaccgAACCCGGCTTATCGGACAGTTGCCCGTGATATCCTAAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGCAGGGGCACAAAGATCAATGACTCGAAACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTa                                                                       <  3:596681/147‑1 (MQ=14)
                                                                               acacAACCCGGCTCATCGGCAATTTTCCCCTCATTTCCTTAATACCG‑CG‑CTTCGCCGGGTTTTTGTGTTTGTCGGCGCCCTACTATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTAc                                                                      <  6:436519/151‑1 (MQ=9)
                                                                                   aaCCCGGCTTCTGGGCCATTTGCGCGTGATTTACGTAACACCG‑CG‑CTGCTGCGGGTTTTTGCTTTTGTGGGGGCCCAACGATGAATGACTCGCCACGACGCTTTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaa                                                                  <  4:1332634/151‑1 (MQ=9)
                                                                                   aaCCCGGCTTATGGGTAAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGGTTTTGCTGGGGCTTAAAGATGATTGACTTTCCACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaa                                                                  <  4:1110965/151‑1 (MQ=14)
                                                                                    aCCCGGCTTATCGGTCATTTTCACGTCATTTACGTAAAAACG‑GC‑CTTCTGCGGGTTTTTGCTTTTGTTGGCGCATAATTATGATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                                 <  2:1236839/151‑1 (MQ=14)
                                                                                      ccGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGc                                                                                                   >  3:886376/1‑115 (MQ=11)
                                                                                            tATGGGTAATTTTCAGGTGATTTAGTTAAAACCC‑GG‑CTGCTGCGGGTTTTTGTTTTTGGTGGTGCTGAACTATGATTGACTTGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGc                                                          <  8:249580/150‑1 (MQ=2)
                                                                                            tATCGGTCAGTTTGTCGTTATTTACGTAAAAACC‑CG‑CTGCGGCGGGTTTTTGCTTTTGTAGGGGCACAAAGATCAAGCACTCGCCACTACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGc                                                          <  1:153971/150‑1 (MQ=11)
                                                                                            tATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTTCTTTTGTAGGGGCAGAAAGATGAATGAGTGTCGACGAAACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGAGCTGGTTGAGGTGAAGAGCCGcg                                                         >  1:113406/1‑150 (MQ=11)
                                                                                            tATCGGTCAGTTTCACCTGATTTACGAAACACCC‑CG‑CTGCGGCGGGTTTTTGCTTTTGTCGGGGCTCAATTTTGAATGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCg                                                           <  1:202148/149‑1 (MQ=11)
                                                                                             atgggtaattttGACCTGATTAACGTAAAAACG‑CG‑CTTCGGCGGGTTTTTGGTTTTGGAGGGGCTGAAATTTGATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCt                                                         <  5:771755/141‑1 (MQ=11)
                                                                                              gcgggaaGTTTCCCGTGATTAACTAAAACCCC‑CG‑CTGCGGGGGTTTTTTGTTTTTGGCGGGGCCCAAATATGAATGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                                       <  7:953048/145‑1 (MQ=11)
                                                                                               ggggaatttgcccgtGATTTA‑GTAAAAACC‑CGTCTGCTGCGGGTTTTTGTTTTTGCCGGCGCCCAACCATGATTGCCTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                      <  1:1205168/137‑1 (MQ=2)
                                                                                               cGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑ATACGGCGGGTTTAAGATTTTTGAGTGCCATAAATAAAAAACACTTTCCACGACACAAAAAACAAAAGCAAACAACATATCGCAAAATATCACATGATATACGTaaaaaaaaaata                                                        >  8:1105325/1‑142 (MQ=255)
                                                                                                 gTCAGTTGCACGTGATTTCCTTAACCACC‑CT‑CTTCGGCGGTTTTTTGTGTTTGTTGGTGTTTAATTTTGATTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc                                                    <  4:280218/151‑1 (MQ=2)
                                                                                                   cAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTAGTAGGGGCAGAAAGATGAATGAGTGTCCACGAAAATATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTGAGGTGAAGAGCCGcgggggggg                                                  >  5:763328/1‑143 (MQ=11)
                                                                                                   cAGTTTCACCTGATCTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATGTACGTaaaaaacagatacga                                                     >  8:701471/1‑138 (MQ=11)
                                                                                                    aGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTGTGCTTTTGGAGGGGCAGTAAGATGAATGACTGTCCACCACGCGATTCCCATAAGAAAGCGGATTAGCGGTCAGTTTGACCTGTTGTACGTAAAAAACAGATGCGGCggg                                                 >  5:688567/1‑151 (MQ=11)
                                                                                                      tttgcggtGATTTACTTAAAACGC‑GT‑CTTAGGGGGGTTTTTGTTTTTGTTGGCGCCGAATTTTGAATGACTTGCCACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGt                                                <  6:21158/143‑1 (MQ=2)
                                                                                                      tttCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGACTGACTGTCCACGCCGCTCTTCCCAAAAGAAAGCGGCTGATCGGTCAGATTCACCTGGATTACGTAAAAACCAGCATCGGCGGGtt                                               >  1:1328702/1‑151 (MQ=11)
                                                                                                       ttCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCGACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATGCGGCGGGttt                                              >  7:1078882/1‑151 (MQ=255)
                                                                                                       ttCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGGTACGGCGGGttt                                              >  7:647124/1‑151 (MQ=255)
                                                                                                       gaccccTGA‑TTACGTTAAAACC‑CGTCTCGGGGGGGTTTTGGCTTTGGGCGGGGCGTAATAATGATGCACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGtt                                               <  5:296887/146‑1 (MQ=2)
                                                                                                        gcccttcaTTTACTTAAAACCC‑CT‑CTGCGGCGGGTTTTTGTTTTGGTCGGGGCTCAACTATCATTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttt                                              <  8:618390/143‑1 (MQ=11)
                                                                                                         cACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACGTGGTGTACGTAAAAAACCGGGTCGGCGGGtttct                                            >  3:1451097/1‑149 (MQ=11)
                                                                                                              gATTTCCGTAAAACCC‑CG‑CGTCGGCGGGTTTTTGGTTTTGCCGGCGCCCAAAGATGAAGGCCTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                       <  3:1468790/151‑1 (MQ=11)
                                                                                                               aTTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCCCGCCGCTATACGCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttgt                                      >  7:1211704/1‑149 (MQ=255)
                                                                                                                  accgaaaacaCC‑CG‑CGGCCGCGGGTTTTTGGTTTTGGCGGCGCACAAACATCAAGGCCTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTgg                                    <  7:310627/145‑1 (MQ=11)
                                                                                                                    cGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGCAAGAGGACTGCCTGTCCACGACGCTATACCCCGAAGAACGCGGCTGATCGGGCAGTTTCCCCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGGTGTTGGCgg                                 >  3:506756/1‑151 (MQ=11)
                                                                                                                        aaaaCC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCCCGACCACGTAACCGAACCACTGGGGCGTAGCGGGCAGTTTAAGCTGGTTTACTTAAAAAACCGCGTCCGCCGGTATTTCGGGTTGGAgggggc                             >  7:1409028/1‑149 (MQ=2)
                                                                                                                          aaCG‑CG‑CTGCGCCGGGTTTTTGCTGTTGGCGGCGCCCACTCTTGCAGGCCTGGCCACGACTCTATACCCAACAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                           <  5:1537774/151‑1 (MQ=11)
                                                                                                                             cccgccTTCTGCGGGTTTTTGTTTTTGTTCGCGCTATAACTTGATTGACTTGCGTCTACGCTATCCCCTAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaa                          <  8:391223/146‑1 (MQ=2)
                                                                                                                              ccttctttcgcggGTTTTTGTTTTTGTTCGTGTATAATTTTGATTGACTTTCAAATAGGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaaa                         <  6:980946/142‑1 (MQ=11)
                                                                                                                                   ttCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAAAGAAAGTAAAAGACTCTATAACCAAAAGAAAGAGCCTAAAAGTTAAGATAAAGCTGGTTTACGTAAAAAAAAAAAAAAAAGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgg                    >  2:329267/1‑150 (MQ=2)
                                                                                                                                     cggcggGTTTTTGGTTTTGCCGGGGCCCACCGATGAAGGCCTGTCCACGACGCTATACCCAACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                  <  3:1145613/151‑1 (MQ=11)
                                                                                                                                     ccgcggGTTTTTGGTTTTGTCGGGGCGAACAATTGATTGACTTGCCAAGACGCTATCCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                  <  1:1392386/149‑1 (MQ=11)
                                                                                                                                             ttttGTTTTTGGCGGGGCATACCGTTGAATGACTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTc           <  7:545283/150‑1 (MQ=11)
                                                                                                                                                     ttGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCt                                                                                               >  3:780108/1‑58 (MQ=35)
                                                                                                                                                     ttGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCGGGTTTAAGCTTTTTGAGTGGCAGAAAGAAAAATCAATGTCAAAGacaat  >  8:1386660/1‑149 (MQ=11)
                                                                                                                                                        |                                                                                                                                                   
AAGGTACGGCCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑CG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACT  >  DH1/613439‑613736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑