Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,5970GC28.3% 281.0 / 38.7 206intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base G (56/63);  new base C (4/43);  total (66/140)
Fisher's exact test for biased strand distribution p-value = 1.63e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

CCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAA  >  DH1/613448‑613745
                                                                                                                                                        |                                                                                                                                                     
cccGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                                                                                                                                                      <  6:1125992/151‑1 (MQ=255)
cccGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                                                                                                                                                      <  3:45428/151‑1 (MQ=255)
  cGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCATCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaaa                                                                                                                                                    <  8:177293/151‑1 (MQ=255)
  cGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaa                                                                                                                                                     >  7:645696/1‑150 (MQ=255)
   gTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAg                                                                                                                                                   <  5:356470/151‑1 (MQ=255)
    tACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACACAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAGACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaa                                                                                                                                                     >  7:296044/1‑148 (MQ=255)
     aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAGCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑at                                                                                                                                                <  5:1531549/151‑1 (MQ=255)
     aCTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACACGCAATATAGGTCACTTTAACCAGATTTACGTAAAAAAA‑C‑G‑CTTCTGCTGGGTTTTCCTTTTGGAGGGAAAGaa                                                                                                                                                     >  5:225420/1‑147 (MQ=255)
      cTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACACGGATTATCGGTCAGTATCGCAAGATTTAAGTAAAAACC‑A‑G‑CTTAGGCGGGTTTTTCGTTTTGGAGGGGCAGAAAG‑att                                                                                                                                               >  5:1198989/1‑150 (MQ=255)
        gatccggggttgtgtgctTGATGGAGTGATCGATTGCTGGCCTTGATTAATGACTTTACACTACAGAACGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAG‑at                                                                                                                                                <  6:1000656/134‑1 (MQ=14)
        gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑atgaa                                                                                                                                             <  7:1444314/151‑1 (MQ=255)
        gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑atga                                                                                                                                              <  4:1027333/150‑1 (MQ=255)
        gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑atg                                                                                                                                               <  3:1050381/149‑1 (MQ=255)
        gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTCCTTTTGGAGGTGCAGAAAG‑atca                                                                                                                                              >  4:519546/1‑148 (MQ=255)
        gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGAAAAAACA‑A‑G‑CTACGCCGGGTTTTTCATTTTGTAGGGGCATAAAG‑atga                                                                                                                                              >  8:545635/1‑150 (MQ=37)
        gAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACACGCCTTATCGATCAGTTTCACCTGATTTACGTAAAAACC‑A‑G‑CTGCGGCGGGTTTTTGGTTTTGGAGGGGCAGAAAG‑atga                                                                                                                                              >  1:1492815/1‑150 (MQ=37)
        gAACCCGGGGAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑atga                                                                                                                                              <  4:1369330/150‑1 (MQ=255)
         aaCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑atgaat                                                                                                                                            <  2:511011/151‑1 (MQ=255)
         aaCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTCCTTTTGGAGGGGCAGAAAG‑atgaa                                                                                                                                             >  4:254016/1‑150 (MQ=255)
          aCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑atgaatg                                                                                                                                           <  3:1241658/151‑1 (MQ=255)
          aCCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCATCGGCATATAGATTTTGAAGGTGAAGAAAT‑aagaat                                                                                                                                            >  4:105995/1‑150 (MQ=37)
             cGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACt                                                                                                                                        <  1:1431531/151‑1 (MQ=255)
               ggTAGGCTGCTTGAGCCAGTGAGCGAGTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGt                                                                                                                                      <  8:65951/151‑1 (MQ=255)
                 tAGGCTGATTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑A‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGTGGCATATAT‑TTGAATCGCTGTcc                                                                                                                                    >  7:1016975/1‑151 (MQ=25)
                    gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAGCCGGCTTATAGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTAGGCGGGTTTTTGCTTTTGGAGGGGCTGAAAG‑ATGAATGAATGTCCacg                                                                                                                                 >  5:1319323/1‑151 (MQ=255)
                    gctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCacg                                                                                                                                 <  3:182743/151‑1 (MQ=255)
                     ctgctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACACAAAACCCATTATCAATCACTTTAACCAGAATTACATAAAAACA‑C‑T‑CTTCCTCGAGTTTTTGATTTTGGAGGTGCAGAAAG‑ATGAATAACtataaacg                                                                                                                                 >  2:535243/1‑143 (MQ=255)
                       gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCacgacg                                                                                                                              <  2:795952/151‑1 (MQ=255)
                       gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCacgac                                                                                                                               <  3:646174/150‑1 (MQ=255)
                         ttGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACACGGCTTATCCGTCAGTTTCAGCTGATTAACGTAAAAACC‑C‑C‑CTTCGGCCCCTTTTTGGTATTGCAGGTGCAGAAAG‑ATGAATGACTGTCCACGACCCt                                                                                                                            >  1:1045228/1‑151 (MQ=21)
                          tGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAACCGGCTTATATCTCAATTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCATCTGGTTTTTTCTTTTGGAGGGGCAGAAAT‑ATGAATGACTGTCCACGACCCt                                                                                                                            >  2:922773/1‑150 (MQ=255)
                            cgCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAT‑ATGAATGACTGTCCACGACGCtata                                                                                                                         <  1:877927/150‑1 (MQ=255)
                              ccAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAAACCGCTAATAAGACAGTTTAAAATGATTTAAGTAAAAAAC‑A‑A‑CTTCTAAATGTTTTTGCTATTGGATTGACAGAAAT‑ATGAATGACTGTACACGACACTATAcc                                                                                                                       >  8:1323907/1‑151 (MQ=255)
                                   gAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGGTTCACCTGATTTACGTAAAAACC‑A‑G‑CTTCCGCGCGTTGTTGCTTTTGTAGGGGGAGAAAG‑ATGAATGAATGTCCACGACACTATACGCaaa                                                                                                                   >  7:162495/1‑150 (MQ=18)
                                   gAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAAACCCCTAATAAATCAATTTCACATGATATACGTAAAAACA‑A‑G‑CTTAGGCGGGTTTTTGCTTTAGGAGGGGCATAAAG‑ATGAATGAATATAAAAGACACTATACCCaaa                                                                                                                   >  8:78874/1‑150 (MQ=12)
                                   gAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAAACCCCAAATCAATCAGATTCAAATGAATTACGTAAAAACA‑A‑G‑CTTCTGCCGCTTTTTGATTTTGTAGGTGCAGAAAG‑ATGAATTAATATAAAAGACACTATACCCaaa                                                                                                                   >  2:822779/1‑150 (MQ=12)
                                      cGATGGCTGGCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTAGCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAaaagaa                                                                                                               <  4:197084/151‑1 (MQ=255)
                                       gATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAaaagaa                                                                                                               >  7:85753/1‑150 (MQ=255)
                                        aTTGCTGGCCTTGATGAATGACTTTCCACTACAGAACCCGGCTTATCGTTCATTTTCACCTGATTTACTTAAAAACC‑C‑T‑CTTCGGCGGTTTTTTGATTTTTTAGGTTATTAAAT‑ATGAATTACTGTCCACGACGCTATACCCAaaagaaa                                                                                                              <  6:466724/150‑1 (MQ=255)
                                        aTTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCCGCTTATCGGTCAGTTTCACGGGGTTTACGTAAAAACC‑C‑G‑ATTCGGCGGGGTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATTACTGTCCACGACGCTATACCCGaaagcacg                                                                                                             >  3:592702/1‑147 (MQ=18)
                                            cTGGCCTAGATGAATGACTGTCCACGACAGAAAACGCATTATAGCTCCGTTTCACATGATTTAAGTAAAAAAC‑A‑C‑CTTCGGCGGGTTTTTGGTTTTGGAGGGGCAGAAAG‑ATGAATGAATGTCCACGACGCTATACCCCACAGAAAGCggg                                                                                                         >  7:344998/1‑150 (MQ=17)
                                              ggCCTATATGAATGCCTGGCCACCACATAACCCGGCTTAGGGGTCAGTTTCACCTTATTTCCGTAAAACCC‑C‑G‑CTTCGGCGGTTTTTTGGTTTTGTAGGGGCACAAAG‑ATGAATGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCtt                                                                                                       <  7:93206/151‑1 (MQ=14)
                                                   ggacaactccctagccaccaCAGAACGCGGTTTATCGGTCAGTTTCACCTTTTTTACTTAAAAACC‑G‑G‑CTTCGGCGGGTTTTTGGTTTTGCTGGGGCCCAAAG‑ATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCg                                                                                                   <  1:850937/136‑1 (MQ=11)
                                                   acattactgaCTGTCCACTACAGAGCCCTGATTAGCGGTCATTTTCACCTGATTTACTTAAAAACC‑C‑T‑CTTCGGCGGGTTTTTGCTTTTGTATGGGCACAAAG‑ATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                                   <  6:1428644/143‑1 (MQ=14)
                                                     atgaatgaCTTACCACGACAGAACCCGTCTTATCGGTATGTTTCTCCTGATTTACTTAAAAACC‑C‑G‑CTGCGGCGGGTTTTTTTTTTTGGTGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                                 <  1:1068886/150‑1 (MQ=17)
                                                     atgaatgaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCAACTGATTTACGTAAAAAAC‑C‑G‑CTTCGGCGTGTTTATGATTTTGGAGGTGCATAAAG‑ATGAATGACTTTCCACAACAATAAAAACAAAAGAAAGCGGCTTATCGGTc                                                                                                >  6:795193/1‑151 (MQ=17)
                                                      tgaatgaCTGTCCACGACAGAAACCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑T‑CTTCGTCGGGATTTTGCTTTTTGAGGGTCATAAAC‑ATGAATGAATAACAAAGAATATATACCAAAAAGAAAGCGGCTTATCGGTc                                                                                                >  4:1201592/1‑150 (MQ=14)
                                                      taactgaCTCTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAG‑a                                                                                                                                                 <  3:333176/97‑1 (MQ=255)
                                                         atgaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACCCTATACCCAaaggaaaaccggagaggggtc                                                                                                >  4:1532065/1‑133 (MQ=17)
                                                           gaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCa                                                                                                                                   >  4:1148355/1‑110 (MQ=255)
                                                           caCTGGCCACCACAGAACCCGGCTAATCGGCCAGTTTCCCCTGATTTACTTAAAACCC‑C‑G‑CTGCGGCGGGTTTTTGCTTTTGCAGGGGCACAAAC‑ATCAAGGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTc                                                                                          <  7:123239/150‑1 (MQ=14)
                                                             cTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAACC‑A‑C‑CGTAGACCGTATTTTACTTTAAGAGTGTCACAAAA‑ATTAATGAAATACGACGACGCAATAACCAAAAAAAAGAGGCTTATCGGTCAgggtg                                                                                          >  2:1453167/1‑145 (MQ=255)
                                                             cTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAAC‑C‑T‑CTTAAGCGCGTATTTTATTTAAAAAGGGCAGAAAT‑ATGAATAACTGTCCAAGAACCTATAAACAAAAAAAAAAAGCTTATCGGTCAGTTTGAc                                                                                        >  2:387722/1‑151 (MQ=255)
                                                               cgccAGGACAGAACCCGGCTTAGCGGCCAGTTGCACTTG‑TTTTCGTTAAAACCGC‑G‑CTTCGGCGGGTTTTTGCTTTTGTTGGCGCTCAAAT‑ATGATGGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCt                                                                                      <  6:286567/149‑1 (MQ=9)
                                                                cccACGACACAACCCGGCTTCTCGGGCAGTTTCACCTGATTAAGGAAAAACCC‑C‑G‑CTGCGGGGGTTTTTTGGTTTTGGAGGGGCACAAAG‑ATCAATCACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTg                                                                                     <  7:537048/150‑1 (MQ=14)
                                                                 cctctactgAACCCTGATTATGGGTAATTTTCTCGTCATTTA‑GTTAAAACC‑G‑GTCTGCTGCGGGTTTTTGTTTTTGTTGGTGGTCAATG‑ATGAATGACTTTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTgg                                                                                    <  2:286551/143‑1 (MQ=2)
                                                                  caccacACAACCCGGCTTATGGTTAATTTTCACTTGATTTACTTAAAAACG‑G‑T‑CTGCTGCGGTTTTTTGTTTTTGTTGGTGTTTAATT‑ATGATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGt                                                                                   <  2:1226206/151‑1 (MQ=9)
                                                                  cACGACAGAACCCGGATTAGCGGTAAGTTTCACGTGATTTACTTAAAACCG‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGTGGGGCACAACC‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGt                                                                                   <  3:844758/151‑1 (MQ=14)
                                                                    ggACAGAACCCGGCTTATCGGGCAGTTTCACCTGATTTACGTAAAAACC‑G‑T‑CTGCGGCGGGTTTTTGCTTTTGGTGGCGCTTAAAG‑ATGACTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGttt                                                                                 <  3:564074/150‑1 (MQ=14)
                                                                      acacAACCCGGCTCATCGGCAATTTTCCCCTCATTTCCTTAATACCG‑C‑G‑CTTCGCCGGGTTTTTGTGTTTGTCGGCGCCCTACT‑ATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTAc                                                                               <  6:436519/151‑1 (MQ=9)
                                                                       cAGACCCCGGCTTCGCGGTCAGTTTCACCTCATTAACTAAAAACCC‑C‑G‑CTGCGGCGGTTTTTTGGTTTGGGCGGGGCACAAAG‑ATGAATGACGGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACg                                                                              <  8:568327/151‑1 (MQ=14)
                                                                        aGACCCCGGGTTATCGGGAATTTTCACGTGATTTACGTAAAACCC‑C‑C‑CTGCGGCGGGTTTTTGCTTTTGGAGGGGCACAAAG‑TTGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGt                                                                             <  1:527201/151‑1 (MQ=14)
                                                                          aaCCCGGCTTCTGGGCCATTTGCGCGTGATTTACGTAACACCG‑C‑G‑CTGCTGCGGGTTTTTGCTTTTGTGGGGGCCCAACG‑ATGAATGACTCGCCACGACGCTTTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaa                                                                           <  4:1332634/151‑1 (MQ=9)
                                                                            cccGGATTATGTGTAAGTTTCACCTCATTTAAGTAAAAACC‑G‑G‑CTTCTGCTTGTTTTTGGTTTTGCAGGTTAATAAAG‑ATGACTTACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaa                                                                         <  7:1293733/151‑1 (MQ=11)
                                                                             ccGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACACTATACCCAAAAGAAAGc                                                                                                            >  3:886376/1‑115 (MQ=11)
                                                                              cGGCTTAGCGGGAATTTGCACGTCATTAACTAAACAACG‑G‑G‑CTTCTGCGGTTTTTTGGTTTTGGAGGGGCACAAAG‑ATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAc                                                                       <  1:267184/151‑1 (MQ=11)
                                                                                gCTTATGGGTATTTTTCAGGTTATTTACG‑AAAAACC‑G‑GCCTGCGGGGGTTTTTTGGTTTTGCTGCCGGATAAAT‑TTGATTGAGTTGCCAAGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAccc                                                                     <  7:460031/151‑1 (MQ=2)
                                                                                   tatggcgaactttCTCCTGATTTACTTAATACCG‑G‑T‑CTTCGGCGGGTTTTTGTTTTTGTTGGTGGCCTATC‑ATGAATGACTTGCCAAGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGc                                                                   <  2:1340191/140‑1 (MQ=2)
                                                                                   tATGGGTAATTTTCAGGTGATTTAGTTAAAACCC‑G‑G‑CTGCTGCGGGTTTTTGTTTTTGGTGGTGCTGAACT‑ATGATTGACTTGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGc                                                                   <  8:249580/150‑1 (MQ=2)
                                                                                   tATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTTCTTTTGTAGGGGCAGAAAG‑ATGAATGAGTGTCGACGAAACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGAGCTGGTTGAGGTGAAGAGCCGcg                                                                  >  1:113406/1‑150 (MQ=11)
                                                                                    atgggtaattttGACCTGATTAACGTAAAAACG‑C‑G‑CTTCGGCGGGTTTTTGGTTTTGGAGGGGCTGAAAT‑TTGATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCt                                                                  <  5:771755/141‑1 (MQ=11)
                                                                                     gcgggaaGTTTCCCGTGATTAACTAAAACCCC‑C‑G‑CTGCGGGGGTTTTTTGTTTTTGGCGGGGCCCAAAT‑ATGAATGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                                                <  7:953048/145‑1 (MQ=11)
                                                                                      ggggaatttgcccgtGATTTA‑GTAAAAACC‑C‑GTCTGCTGCGGGTTTTTGTTTTTGCCGGCGCCCAACC‑ATGATTGCCTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                               <  1:1205168/137‑1 (MQ=2)
                                                                                      cGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑ATACGGCGGGTTTAAGATTTTTGAGTGCCATAAAT‑AAAAAACACTTTCCACGACACAAAAAACAAAAGCAAACAACATATCGCAAAATATCACATGATATACGTaaaaaaaaaata                                                                 >  8:1105325/1‑142 (MQ=255)
                                                                                          cAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTAGTAGGGGCAGAAAG‑ATGAATGAGTGTCCACGAAAATATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTGAGGTGAAGAGCCGcgggggggg                                                           >  5:763328/1‑143 (MQ=11)
                                                                                          aattttCACCTGATTT‑CCTAAAACCC‑CGG‑CTTCGGCGGGTTTTTGGTTTTGGCGGCGCACACCC‑ATGATTGACTCGCCACTACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggcgg                                                           <  5:918278/148‑1 (MQ=11)
                                                                                           aGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTGTGCTTTTGGAGGGGCAGTAAG‑ATGAATGACTGTCCACCACGCGATTCCCATAAGAAAGCGGATTAGCGGTCAGTTTGACCTGTTGTACGTAAAAAACAGATGCGGCggg                                                          >  5:688567/1‑151 (MQ=11)
                                                                                             tttCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGACTGACTGTCCACGCCGCTCTTCCCAAAAGAAAGCGGCTGATCGGTCAGATTCACCTGGATTACGTAAAAACCAGCATCGGCGGGtt                                                        >  1:1328702/1‑151 (MQ=11)
                                                                                              ttCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCGACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATGCGGCGGGttt                                                       >  7:1078882/1‑151 (MQ=255)
                                                                                                cACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACGTGGTGTACGTAAAAAACCGGGTCGGCGGGtttct                                                     >  3:1451097/1‑149 (MQ=11)
                                                                                                    tGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGCCTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTGTGACCTGGTGTACGTAATAAGCCGGTCCGGCGGGTTTGAGCtt                                                 >  5:265368/1‑151 (MQ=11)
                                                                                                     gATTTCCGTAAAACCC‑C‑G‑CGTCGGCGGGTTTTTGGTTTTGCCGGCGCCCAAAG‑ATGAAGGCCTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                <  3:1468790/151‑1 (MQ=11)
                                                                                                     gATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGGGGCGTAGCGGTCAGTTTCACGTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                >  7:600102/1‑151 (MQ=255)
                                                                                                     gATTTACGAAAAACCC‑C‑G‑CTGCGGCGGGTTTTTGCTTTTGCCGGGGCACAACC‑ATCAAGGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                <  7:651497/151‑1 (MQ=11)
                                                                                                     caTTTACTAAAAACCC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGTTGGGGCTCAAAC‑ATGAAGGACTCTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCt                                                  <  6:844882/148‑1 (MQ=11)
                                                                                                      aTTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCCCGCCGCTATACGCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttgt                                               >  7:1211704/1‑149 (MQ=255)
                                                                                                           cGTAACACCC‑C‑G‑CTGCGGCGGGTTTTTGGTTTTGGAGGGGCCCAAAG‑ATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAgg                                          <  5:667764/151‑1 (MQ=11)
                                                                                                           cGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGCAAG‑AGGACTGCCTGTCCACGACGCTATACCCCGAAGAACGCGGCTGATCGGGCAGTTTCCCCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGGTGTTGGCgg                                          >  3:506756/1‑151 (MQ=11)
                                                                                                               aaaaCC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCCCGACCACGTAACCGAACCACTGGGGCGTAGCGGGCAGTTTAAGCTGGTTTACTTAAAAAACCGCGTCCGCCGGTATTTCGGGTTGGAgggggc                                      >  7:1409028/1‑149 (MQ=2)
                                                                                                                    aaccgccTTCTGCGGGTTTTTGCTTTTGTACGTGACGACAT‑ATCATTGACTTTCCCAGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                                    <  8:1042186/145‑1 (MQ=11)
                                                                                                                     cccggctgccgcggGTTTTTGATTTTGCAGGCGCCTAAAG‑ATGAATGACTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaa                                   <  1:169147/141‑1 (MQ=11)
                                                                                                                            tCGCCGGGTTTTTGCTTTTGGCGCGGCCCAACG‑ATGAAGGACTGCCCACGACGCTATACCCAACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                              <  4:405982/149‑1 (MQ=11)
                                                                                                                             cggcggGTTTTTGGTTTTGCCGGGGCCCACCG‑ATGAAGGCCTGTCCACGACGCTATACCCAACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                           <  3:1145613/151‑1 (MQ=11)
                                                                                                                             ccgcggGTTTTTGGTTTTGTCGGGGCGAACAA‑TTGATTGACTTGCCAAGACGCTATCCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                           <  1:1392386/149‑1 (MQ=11)
                                                                                                                              ggcggGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTACAACACAATATACACATATAAAAAAAGCTTATCGCTCAGTTTCAACTGGTTTAAGTAAAAACAATCTTATAAATAATTTTGCTTTTGGAGGGGCAGAAAGatgaatg                          >  2:1496282/1‑151 (MQ=2)
                                                                                                                              gccggGTTATTGGTTTTGAAGGAGC‑GAAAGAATGATTGCCTTGACACCACGCTCTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg                          <  5:1029871/149‑1 (MQ=11)
                                                                                                                                 gggTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATTTACGTAATAAACCGATCCGGCGGGTTTCAGATTTTTGAGTGCCAGatataaataacac                        >  2:206286/1‑138 (MQ=14)
                                                                                                                                             ttGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCGGGTTTAAGCTTTTTGAGTGGCAGAAAGAAAAATCAATGTCAAAGacaat           >  8:1386660/1‑149 (MQ=11)
                                                                                                                                                  ggggCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCGGGTTTAAGATTTTTGAGTGACATAAAAATAAATTCCTGTACACAAAAATATAcc      >  8:33611/1‑151 (MQ=2)
                                                                                                                                                       aGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATACGGCGGGTTTTAGATTTTTGAGTGCCATAAAGAGGTAAGACAGTCCACGAGACtgaagacaca  >  8:462090/1‑141 (MQ=11)
                                                                                                                                                        |                                                                                                                                                     
CCCGTACTGAACCCGGGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACC‑C‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAA  >  DH1/613448‑613745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑