Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,6130TG24.8% 177.4 / 23.6 165intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base T (51/67);  new base G (0/39);  total (53/112)
Fisher's exact test for biased strand distribution p-value = 1.74e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGG  >  DH1/613463‑613762
                                                                                                                                                         |                                                                                                                                                     
ggTAGGCTGCTTGAGCCAGTGAGCGAGTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGt                                                                                                                                                       <  8:65951/151‑1 (MQ=255)
  tAGGCTGATTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCA‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGTGGCATATAT‑TTGAATCGCTGTcc                                                                                                                                                     >  7:1016975/1‑151 (MQ=25)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCacgacg                                                                                                                                               <  2:795952/151‑1 (MQ=255)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCacgac                                                                                                                                                <  3:646174/150‑1 (MQ=255)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCacgac                                                                                                                                                <  2:1020381/150‑1 (MQ=255)
        gctTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCacga                                                                                                                                                 <  7:1227848/149‑1 (MQ=255)
          ttGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACACGGCTTATCCGTCAGTTTCAGCTGATTAACGTAAAAACCC‑C‑CTTCGGCCCCTTTTTGGTATTGCAGGTGCAGAAAG‑ATGAATGACTGTCCACGACCCt                                                                                                                                             >  1:1045228/1‑151 (MQ=21)
           tgcgCCAGTGAGCCATTCCTGCCCTAGATGAATGACTGTCCACGACAGAACCGGGCTTAGCGGTCAGTTTCACCTTATTTACTTAAAAACCT‑G‑CTTCGGGGGTTTTTTGTTTTTGTAGGGTCATAAAT‑ATTAATTACTGTCCACGACGCt                                                                                                                                             <  4:440201/147‑1 (MQ=18)
           ggAGCCAGGGAGCCATGGCTGGCCTAGCTGAATGACGGTCCACGACAGAACCCGGCTTATCGGGCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑AGGAATGACTGTCCACGACGCta                                                                                                                                            <  2:932509/150‑1 (MQ=18)
              gCCAGTGTGCGATTGGTGGCCTAGATTAATCAGTGTCCTGGACAGAACCCGGCTTATCGTTCATTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGTTTTTGTAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCtata                                                                                                                                          <  4:224866/150‑1 (MQ=17)
               ccAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACTCTATAcc                                                                                                                                        >  7:1501260/1‑151 (MQ=255)
                cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTTATGTAACTAAAAACCC‑G‑AATCGCCTGGTTTTTGCTATTTGAGTGGCTCAAAG‑ATGACTGCCTGTCCTAGACACtaacac                                                                                                                                        >  6:822029/1‑146 (MQ=19)
                cAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATAGGTCAGTTTAACCTGATTTACGTAAAAACCA‑G‑CTTAGGCGGGTGTTTGGTTTTGGAGGGGCAGATAG‑ATGAATGACTGTCCACGACGCTATAccc                                                                                                                                       >  5:236888/1‑151 (MQ=21)
                    gAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAAACCCCTAATAAATCAATTTCACATGATATACGTAAAAACAA‑G‑CTTAGGCGGGTTTTTGCTTTAGGAGGGGCATAAAG‑ATGAATGAATATAAAAGACACTATACCCaaa                                                                                                                                    >  8:78874/1‑150 (MQ=12)
                    gAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAAAACCCCAAATCAATCAGATTCAAATGAATTACGTAAAAACAA‑G‑CTTCTGCCGCTTTTTGATTTTGTAGGTGCAGAAAG‑ATGAATTAATATAAAAGACACTATACCCaaa                                                                                                                                    >  2:822779/1‑150 (MQ=12)
                     cgCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAaaag                                                                                                                                  <  4:154388/150‑1 (MQ=255)
                         aTTGCTGGCGTAGATCAATGACTGTCCCCCACAGACCCCGGCTTAGCGGCCTGTTTCACCTGATTTACGTAAAACCCG‑G‑CTTCGGGGGGTTTTTGGTTTTGGAGGGGCAGAAAG‑ATCAATGACTGGCCACGACGCTATACCCAaaagaaa                                                                                                                               <  7:1303324/150‑1 (MQ=255)
                         aTTGCTGGCCTTGATGAATGACTTTCCACTACAGAACCCGGCTTATCGTTCATTTTCACCTGATTTACTTAAAAACCC‑T‑CTTCGGCGGTTTTTTGATTTTTTAGGTTATTAAAT‑ATGAATTACTGTCCACGACGCTATACCCAaaagaaa                                                                                                                               <  6:466724/150‑1 (MQ=255)
                         aTTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACGGGATTTACGTAAAAACGC‑G‑CTTCGGCGGGGTTTTGCTTTTGGAGTGTCAGAAAG‑ATGAATTACTGTCCACGACCCTATACCCAaaagaa                                                                                                                                >  3:585860/1‑149 (MQ=18)
                            ggtGGCCTAGATGAATGACGGTCCACCACACAACCCGGGTTAGCGGTCAGTTTCACGTGATTTACGAAAAACCCC‑G‑GTGCGGGGGGTTTTTGCTTTTGGCGGGGCAGAAAG‑ATGAATGAGTGTCCACGACGCTATACCCAAAAGAAAGCgg                                                                                                                           <  7:1324015/149‑1 (MQ=17)
                              tGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCA‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGACAGCGGcg                                                                                                                         >  3:206870/1‑150 (MQ=255)
                               ggCCTAGTTTAATGACTGTCCACGACAGAACCGGGCTTATGGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTGGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCt                                                                                                                         <  1:141358/150‑1 (MQ=17)
                               ggCCTAGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACTTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCt                                                                                                                         <  7:484432/150‑1 (MQ=255)
                                gCCTAGATGAATGACTGCCCACGACAGAACCCGGGTTAGCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGGTTTTGGAGGGGCAGAAAG‑ATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTa                                                                                                                       <  7:259232/151‑1 (MQ=255)
                                 ccTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGTTTTTTGCTTTTGTCGGGGCACAAAT‑ATCAATGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTAt                                                                                                                      <  8:206981/151‑1 (MQ=18)
                                    ggacaactccctagccaccaCAGAACGCGGTTTATCGGTCAGTTTCACCTTTTTTACTTAAAAACCG‑G‑CTTCGGCGGGTTTTTGGTTTTGCTGGGGCCCAAAG‑ATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCg                                                                                                                    <  1:850937/136‑1 (MQ=11)
                                    atattattgaCGGCCCACGACAGACCCCGCTTTATCGGTTTTTTTCACTTTATTTACTTAAAATCCG‑G‑TTTGGGGGGTTTTTTGCTTTTTGATGGCAAAAAAT‑ATCAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCgg                                                                                                                   <  8:1380392/144‑1 (MQ=9)
                                    acattactgaCTGTCCACTACAGAGCCCTGATTAGCGGTCATTTTCACCTGATTTACTTAAAAACCC‑T‑CTTCGGCGGGTTTTTGCTTTTGTATGGGCACAAAG‑ATTAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                                                    <  6:1428644/143‑1 (MQ=14)
                                    aGATGAATGACTGTCCCCGACACAACCCGGCTTAGGGGTCAGTTTCACCTGATTTACGTAAAACCCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCgg                                                                                                                   <  5:1256710/151‑1 (MQ=255)
                                    aGATGAATGACTGGCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCATAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCgg                                                                                                                   <  6:647398/151‑1 (MQ=255)
                                    aGATGAATGACTGACCACGACAGACCCCGGGTTATCGGTCAGTTTCACCTGATTTACGTAAAACCCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGCGGGGCAGAAAG‑ATGAATGACTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCg                                                                                                                    <  7:924350/150‑1 (MQ=17)
                                     tattaattagtGTCCCCGACAGAACCCGGCTTTTCGTTCAGTTTCACATGATTTACTTAAAAACCG‑G‑CTGCTGGGGTTTTTTGGTTTTGTAGGGGAATAAAG‑TTTACGACCTGTCCACGACGGTATACCCAAAAGAAAGCGGCTTATCgg                                                                                                                   <  7:458102/140‑1 (MQ=9)
                                     gATTAATGACTGACGACTACAGACCCCTGTTTATCTTTCAGTTTAACCTGATTTACGTAAAAACCC‑T‑CTTGTGCGGGTTTTTGCTTTTGTAGGGGCTTAAAC‑ATTATTGACTGTCCACTACGCTATACCCAAAAGAAACCGGCTTATCgg                                                                                                                   <  8:1013224/150‑1 (MQ=255)
                                     gATTAATGACTGACCACGACAGAACCCGCGTTATGGGTCTGTTTCACCTGATTTACTTAAAAACCC‑G‑CTGGGGGGGTTTTTTGGTTTTGGTGGGGCACAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                                                  <  5:500809/151‑1 (MQ=17)
                                     gATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTAGGAAAACACCA‑C‑CTGAGACCGCGATTGTCTAATGGAGGGCCAGAATG‑CTGAATGCGTGTCGAAGACCCTATACCCAAAAGAAAGCggggggggggg                                                                                                                  >  7:125930/1‑142 (MQ=12)
                                     gATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTAAGTAAAAACCC‑G‑CTGCGCAGGGGTTTTGAGTTTGGAGGGGCAGAAAG‑ATGAATTACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                                                  >  7:93612/1‑151 (MQ=17)
                                      tttaatgaCTGTCTACGACAGAACCCGGCTTATCGGTCAGTTTCACCTTATTTAGGTAAAAACCC‑G‑CTTCGGGGGTTTTTTGGTTTTGGAGGGGCAGAAAT‑ATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGt                                                                                                                  <  5:681335/147‑1 (MQ=17)
                                       tgaatgaCTGTCCACGACAGAAACCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑T‑CTTCGTCGGGATTTTGCTTTTTGAGGGTCATAAAC‑ATGAATGAATAACAAAGAATATATACCAAAAAGAAAGCGGCTTATCGGTc                                                                                                                 >  4:1201592/1‑150 (MQ=14)
                                          atgaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACCCTATACCCAaaggaaaaccggagaggggtc                                                                                                                 >  4:1532065/1‑133 (MQ=17)
                                           agacttacctaaacacAACCCGGATTATGGGTAATTTTCTCTTCATTTACTTAAACCCGG‑T‑CTTCTGCGGGTTTTTGTTTTTGTTGGGGCTCAATT‑ATGAATGACTTTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGttt                                                                                                            <  2:583532/139‑1 (MQ=2)
                                            gaCTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTACTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCa                                                                                                                                                    >  4:1148355/1‑110 (MQ=255)
                                             ccTGCCCACTACACAACCCGTCTTATCGGTCATTTTCCGCTGATTTACTTAAAACCCG‑T‑GTTCTGGGTGTTTTTGTTTTTGCTGGGGCTCAATT‑TTTATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCa                                                                                                          <  6:1094683/150‑1 (MQ=9)
                                             acatccccccaCAGAACCCGGCTTATGGGCCATTTGCCCCTGATTTCGTTAAAAACCG‑C‑CTGCGCCGGGTTTTTGTTTTTGCTGGGGCATAAAC‑ATGAATGACTCTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTc                                                                                                           <  2:464710/140‑1 (MQ=9)
                                              cTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTAACCTGATTTACGTAATAACCA‑C‑CGTAGACCGTATTTTACTTTAAGAGTGTCACAAAA‑ATTAATGAAATACGACGACGCAATAACCAAAAAAAAGAGGCTTATCGGTCAgggtg                                                                                                           >  2:1453167/1‑145 (MQ=255)
                                               tGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAT‑ATGAAGGCCTGTGCACTCCGCTCTACACATACGAAAGCGGCTTATCGGTCAGgttgtc                                                                                                         >  5:960228/1‑147 (MQ=14)
                                                 cccACGACACAACCCGGCTTCTCGGGCAGTTTCACCTGATTAAGGAAAAACCCC‑G‑CTGCGGGGGTTTTTTGGTTTTGGAGGGGCACAAAG‑ATCAATCACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTg                                                                                                      <  7:537048/150‑1 (MQ=14)
                                                  cctctactgAACCCTGATTATGGGTAATTTTCTCGTCATTTA‑GTTAAAACCG‑GTCTGCTGCGGGTTTTTGTTTTTGTTGGTGGTCAATG‑ATGAATGACTTTCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTgg                                                                                                     <  2:286551/143‑1 (MQ=2)
                                                   cACGACAGAACCCGGATTAGCGGTAAGTTTCACGTGATTTACTTAAAACCGC‑G‑CTTCGGCGGGTTTTTGCTTTTGGTGGGGCACAACC‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGt                                                                                                    <  3:844758/151‑1 (MQ=14)
                                                      caCAGAACCCGGCTTGTCGGGCAGTTTCACCTGATTTAGGTAAAACCCC‑T‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAACG‑TTGATTGCCTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTa                                                                                                 <  1:1129911/150‑1 (MQ=14)
                                                      aaccgAACCCGGCTTATCGGACAGTTGCCCGTGATATCCTAAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGCAGGGGCACAAAG‑ATCAATGACTCGAAACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTa                                                                                                 <  3:596681/147‑1 (MQ=14)
                                                       acacAACCCGGCTCATCGGCAATTTTCCCCTCATTTCCTTAATACCGC‑G‑CTTCGCCGGGTTTTTGTGTTTGTCGGCGCCCTACT‑ATGAATGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTAc                                                                                                <  6:436519/151‑1 (MQ=9)
                                                         aGACCCCGGGTTATCGGGAATTTTCACGTGATTTACGTAAAACCCC‑C‑CTGCGGCGGGTTTTTGCTTTTGGAGGGGCACAAAG‑TTGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGt                                                                                              <  1:527201/151‑1 (MQ=14)
                                                           aaCCCGGCTTCTGGGCCATTTGCGCGTGATTTACGTAACACCGC‑G‑CTGCTGCGGGTTTTTGCTTTTGTGGGGGCCCAACG‑ATGAATGACTCGCCACGACGCTTTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaa                                                                                            <  4:1332634/151‑1 (MQ=9)
                                                            aCCCGGCTTATGGGTCATTTTCACCTTATTTAGGTAAAAACGC‑C‑CTTCGGCGGGTTTTTGCTTTTGTAGGGGCCCAAAC‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                                                           <  1:1201165/151‑1 (MQ=14)
                                                            aCCCGGCTTATCGGTCATTTTCACGTCATTTACGTAAAAACGG‑C‑CTTCTGCGGGTTTTTGCTTTTGTTGGCGCATAATT‑ATGATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaa                                                                                           <  2:1236839/151‑1 (MQ=14)
                                                             cccGGATTATCGGGCATTTTCACGTCATTAAGGTAAAAACCC‑G‑CTTCGGGGGGTTTTTGCTTTTGGCGGGGCAAAATC‑ATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaa                                                                                          <  1:1463166/151‑1 (MQ=11)
                                                              ccGGCTTATGGCGCAGTTGCACGTCATTAAGGTAAAACCGC‑T‑CTGCGGCGGGTTTTTGCTTTTGTGGGCGCGCAACT‑TTGAATGACTTTCCACGACTCTATACCCAAAAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTaaaaa                                                                                         <  3:533672/151‑1 (MQ=2)
                                                              ccGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACACTATACCCAAAAGAAAGc                                                                                                                             >  3:886376/1‑115 (MQ=11)
                                                               cGGCTTAGCGGGAATTTGCACGTCATTAACTAAACAACGG‑G‑CTTCTGCGGTTTTTTGGTTTTGGAGGGGCACAAAG‑ATGAATGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAAc                                                                                        <  1:267184/151‑1 (MQ=11)
                                                                     atgggtaattttGACCTGATTAACGTAAAAACGC‑G‑CTTCGGCGGGTTTTTGGTTTTGGAGGGGCTGAAAT‑TTGATTGACTTTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCt                                                                                   <  5:771755/141‑1 (MQ=11)
                                                                     atggggcggTTGCACCTGATTAACGTAAACACCC‑G‑ATGCGGCGGGTTTTTGTTTTTGCTGGCGCCCAAAC‑ATGAAGGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCt                                                                                   <  3:104340/142‑1 (MQ=11)
                                                                      gcgggaaGTTTCCCGTGATTAACTAAAACCCCC‑G‑CTGCGGGGGTTTTTTGTTTTTGGCGGGGCCCAAAT‑ATGAATGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                                                                 <  7:953048/145‑1 (MQ=11)
                                                                       gtgtaaGTTGCACCTGATTTAGGTAAAACCCG‑G‑CTTCGGCGGGTTTTTGGTTTTGTTGGGGCATAAAG‑TTGATTGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTc                                                                                 <  3:173842/145‑1 (MQ=11)
                                                                       gggtaattttCACGTGATTTAGGTAAAAAGGG‑G‑ATTCGGTGGGTTTTTGTTTTTGGTGTTGCTTAATT‑TTGATTGACTGTGCACGACGCTATACAGAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACTTAAAAACCCGCTTcg                                                                                <  4:1226947/144‑1 (MQ=2)
                                                                       ggggaatttgcccgtGATTTA‑GTAAAAACCC‑GTCTGCTGCGGGTTTTTGTTTTTGCCGGCGCCCAACC‑ATGATTGCCTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcg                                                                                <  1:1205168/137‑1 (MQ=2)
                                                                       cGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑ATACGGCGGGTTTAAGATTTTTGAGTGCCATAAAT‑AAAAAACACTTTCCACGACACAAAAAACAAAAGCAAACAACATATCGCAAAATATCACATGATATACGTaaaaaaaaaata                                                                                  >  8:1105325/1‑142 (MQ=255)
                                                                         gtaattttCACCTGATTTACGTAAAAACCG‑G‑CTTCGGCGGGTTTTTGGTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggc                                                                              <  3:875195/146‑1 (MQ=255)
                                                                           atgTTTCCCTTGATTT‑CCTAAATACCC‑GTCTTCTGCGGGTTTTTGTTTTTGTTGGTGCTTAATT‑TTGATTGACTTTACACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggcg                                                                             <  8:323271/148‑1 (MQ=2)
                                                                           aattttCACCTGATTT‑CCTAAAACCCCGG‑CTTCGGCGGGTTTTTGGTTTTGGCGGCGCACACCC‑ATGATTGACTCGCCACTACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTcggcgg                                                                            <  5:918278/148‑1 (MQ=11)
                                                                            aGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTGTGCTTTTGGAGGGGCAGTAAG‑ATGAATGACTGTCCACCACGCGATTCCCATAAGAAAGCGGATTAGCGGTCAGTTTGACCTGTTGTACGTAAAAAACAGATGCGGCggg                                                                           >  5:688567/1‑151 (MQ=11)
                                                                              tttgcggtGATTTACTTAAAACGCG‑T‑CTTAGGGGGGTTTTTGTTTTTGTTGGCGCCGAATT‑TTGAATGACTTGCCACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGt                                                                          <  6:21158/143‑1 (MQ=2)
                                                                              tttCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGACTGACTGTCCACGCCGCTCTTCCCAAAAGAAAGCGGCTGATCGGTCAGATTCACCTGGATTACGTAAAAACCAGCATCGGCGGGtt                                                                         >  1:1328702/1‑151 (MQ=11)
                                                                               ttCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCGACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATGCGGCGGGttt                                                                        >  7:1078882/1‑151 (MQ=255)
                                                                               ttCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGGTACGGCGGGttt                                                                        >  7:647124/1‑151 (MQ=255)
                                                                                gcccttcaTTTACTTAAAACCCC‑T‑CTGCGGCGGGTTTTTGTTTTGGTCGGGGCTCAACT‑ATCATTGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttt                                                                        <  8:618390/143‑1 (MQ=11)
                                                                                 cACCTCATTTCCGTAAAACCCC‑G‑CTGCGGCGGGTTTTTGCTTTTGGCGGGGCACAAAC‑ATGAAGGACTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGttttt                                                                      <  1:1066453/151‑1 (MQ=11)
                                                                                     tGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGCCTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTGTGACCTGGTGTACGTAATAAGCCGGTCCGGCGGGTTTGAGCtt                                                                  >  5:265368/1‑151 (MQ=11)
                                                                                      gATTTCCGTAAAACCCC‑G‑CGTCGGCGGGTTTTTGGTTTTGCCGGCGCCCAAAG‑ATGAAGGCCTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                                 <  3:1468790/151‑1 (MQ=11)
                                                                                      gATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGGGGCGTAGCGGTCAGTTTCACGTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                                 >  7:600102/1‑151 (MQ=255)
                                                                                      gATTTACGAAAAACCCC‑G‑CTGCGGCGGGTTTTTGCTTTTGCCGGGGCACAACC‑ATCAAGGCCTGGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttt                                                                 <  7:651497/151‑1 (MQ=11)
                                                                                       aTTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCCCGCCGCTATACGCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCttgt                                                                >  7:1211704/1‑149 (MQ=255)
                                                                                          accgaaaacaCCC‑G‑CGGCCGCGGGTTTTTGGTTTTGGCGGCGCACAAAC‑ATCAAGGCCTGCCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTgg                                                              <  7:310627/145‑1 (MQ=11)
                                                                                            cGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGCAAG‑AGGACTGCCTGTCCACGACGCTATACCCCGAAGAACGCGGCTGATCGGGCAGTTTCCCCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGGTGTTGGCgg                                                           >  3:506756/1‑151 (MQ=11)
                                                                                                  aaCGC‑G‑CTGCGCCGGGTTTTTGCTGTTGGCGGCGCCCACTC‑TTGCAGGCCTGGCCACGACTCTATACCCAACAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGa                                                     <  5:1537774/151‑1 (MQ=11)
                                                                                                     cccggctgccgcggGTTTTTGATTTTGCAGGCGCCTAAAG‑ATGAATGACTCGCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaa                                                    <  1:169147/141‑1 (MQ=11)
                                                                                                     cccgccTTCTGCGGGTTTTTGTTTTTGTTCGCGCTATAAC‑TTGATTGACTTGCGTCTACGCTATCCCCTAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaa                                                    <  8:391223/146‑1 (MQ=2)
                                                                                                      cggtctgaggcggGTTTTTGTTTTTGGCGGCGCTTAATA‑TTGATTGCCTTGCCTAGTCTCTTTACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaaa                                                   <  8:1535962/143‑1 (MQ=2)
                                                                                                      ccttctttcgcggGTTTTTGTTTTTGTTCGTGTATAATT‑TTGATTGACTTTCAAATAGGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGaaa                                                   <  6:980946/142‑1 (MQ=11)
                                                                                                      c‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATACGGCGGGTTTATGATTTTTGAGGGCCatat                                                    >  8:560235/1‑145 (MQ=11)
                                                                                                           ttCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAAAGAAAGTAAAAGACTCTATAACCAAAAGAAAGAGCCTAAAAGTTAAGATAAAGCTGGTTTACGTAAAAAAAAAAAAAAAAGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgg                                              >  2:329267/1‑150 (MQ=2)
                                                                                                            tctgcggGTTTTTGTTTTTGTCGGCGCTCTATT‑ATGATGGACTTGCCACGACGCTATACCCTACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                                               <  8:1060073/146‑1 (MQ=11)
                                                                                                            tCGCCGGGTTTTTGCTTTTGGCGCGGCCCAACG‑ATGAAGGACTGCCCACGACGCTATACCCAACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                                               <  4:405982/149‑1 (MQ=11)
                                                                                                            gctgcggGTTTTTGTTTTTGCCGGTGCCTAACT‑ATGATTGCCTTGCTACGTCTCTATACACCAAAGAACGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatg                                               <  2:1188423/146‑1 (MQ=11)
                                                                                                             cggcggGTTTTTGGTTTTGCCGGGGCCCACCG‑ATGAAGGCCTGTCCACGACGCTATACCCAACAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaat                                            <  3:1145613/151‑1 (MQ=11)
                                                                                                              ggcggGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTACAACACAATATACACATATAAAAAAAGCTTATCGCTCAGTTTCAACTGGTTTAAGTAAAAACAATCTTATAAATAATTTTGCTTTTGGAGGGGCAGAAAGatgaatg                                           >  2:1496282/1‑151 (MQ=2)
                                                                                                              gccggGTTATTGGTTTTGAAGGAGC‑GAAAGAATGATTGCCTTGACACCACGCTCTACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGatgaatg                                           <  5:1029871/149‑1 (MQ=11)
                                                                                                                 gggTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATTTACGTAATAAACCGATCCGGCGGGTTTCAGATTTTTGAGTGCCAGatataaataacac                                         >  2:206286/1‑138 (MQ=14)
                                                                                                                   gTTTTTGCTTTTGGAGGGGCAGAAAG‑ATAAATCACAATCCACTACTCTATACAAAAAATAAAGCTCATTATCTATCACTTTCACCTGGTATAATAAAAAAAAAAAATCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAgatggatgggagg                                      >  8:947033/1‑146 (MQ=2)
                                                                                                                                       aGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATACGGCGGGTTTTAGATTTTTGAGTGCCATAAAGAGGTAAGACAGTCCACGAGACtgaagacaca                   >  8:462090/1‑141 (MQ=11)
                                                                                                                                           aaattgattgcCTTTCCAATACTCTATACCAAAAATAACCCGGCTTATCGGTCATTTTCACGTGGTTTACGTAAAAACCCGATTCGGCGGGTTTTTGGTTTTGGAGGGGCACAAAGATGAATGACTGTCCACGACACTATACCCAaaagaa               <  4:706766/147‑1 (MQ=11)
                                                                                                                                                         |                                                                                                                                                     
GGTAGGCTGCTTGAGCCAGTGAGCGATTGCTGGCCTAGATGAATGACTGTCCACGACAGAACCCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCC‑G‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAG‑ATGAATGACTGTCCACGACGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGG  >  DH1/613463‑613762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑