Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA30.1% 246.9 / 21.1 177intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base C (90/19);  new base A (46/1);  total (156/21)
Fisher's exact test for biased strand distribution p-value = 7.86e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

CGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613620‑613919
                                                                                                                                                      |                                                                                                                                                       
ggCTAAACCCAAAAGAAACACTTTTAGCGATAATTTTACCCTCGATTACGTAATACCGATCTTCGGCGGGTTTTTGCTTTTGGAGGGGCATAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTc                                                                                                                                                         <  8:1085920/150‑1 (MQ=2)
        ccAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGAATAGCCGCGTTTTTGCTAATAGAGTGGCACAAAGATTAAAGAAAGTACAAGCCACTATAAAAAAAAAAAAGCGGCTTATCGGTCAGTTTGACCTGttg                                                                                                                                                 >  8:205733/1‑150 (MQ=2)
              gaaagCGGCTTATCGGTCAGTTTAACCTGGTTTACGTAAAAAACCGATGCGGCGGGTTTATGATTTTTGAGTGCCATAAATAGAAATAACTGTAGACCAGACTATACCCAAAAAAAAGCGCAGTATCGGTAAGTTTCCCATGtagtactt                                                                                                                                            >  8:1541521/1‑143 (MQ=2)
               cacgcgtattATGGGTAATTTTCACATGATTTACTTAAAAACGGGCTGCGCCGGGTTTTTGCTTTTGTAGGCGCAAAATTTTGATTGCCTCGCCACGACACTATACACAAAAGAACGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTaa                                                                                                                                          <  1:830728/143‑1 (MQ=2)
                   cctgggtgcGGGCAGTTGCGCCGGGTGTACGTCCGACCGCGCTTCGGCGGGTTTTTCCTTTTGGAGGGGCAGAAAGATGAATGACTGGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaataaa                                                                                                                                      <  5:111126/143‑2 (MQ=14)
                    ggCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGAGTGTCCACGACACTAGACACAAAAGAAAGCGGCTTATCGGTCAGTTTAAAATGTTTTAAGtaataaac                                                                                                                                     >  1:1194061/1‑149 (MQ=11)
                    ggCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTGCGGCGGGTGTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCAACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGAGCTgggatgcgcacacggc                                                                                                                                     >  3:878647/1‑136 (MQ=11)
                    ggCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACCGATACGGCGGGTTTTTGCTTTTGGAGGGCCATAAATAAGAAACACATTCCACGACAAAATACAAAAAAGCAAAAGACATATCGCACAGTATAAAAAGTTGAACGTAAAAAc                                                                                                                                      >  8:1445601/1‑150 (MQ=2)
                     gCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATAGGTCAGTTTCACCTGTTTTACGTAAAAAacc                                                                                                                                    >  7:967901/1‑151 (MQ=25)
                       ttATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCATATCGGTCAGTTTAACCTGTTGTACGTAAAAAACCgt                                                                                                                                  >  4:543817/1‑150 (MQ=255)
                        tATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTATGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTAAGTTTAACCTGTTTTACGTAAAAACCCGcg                                                                                                                                 >  2:742029/1‑150 (MQ=255)
                          tCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCgatac                                                                                                                               >  6:1328921/1‑147 (MQ=255)
                            ggTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTTcgg                                                                                                                             >  5:385022/1‑151 (MQ=25)
                             gTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACCGCTTcggc                                                                                                                            >  6:571274/1‑151 (MQ=18)
                              tCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGAATTATCGGTCAGTTTAACATGTTATACGTAAAAAACCgatacgccg                                                                                                                           >  2:24892/1‑143 (MQ=11)
                                aGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAACCCGGTGCGGCggg                                                                                                                         >  4:1287307/1‑151 (MQ=255)
                                     cACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGCCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGtttgt                                                                                                                    >  3:234402/1‑149 (MQ=255)
                                     cACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTGTACGTAATAACCCGTTGCGGCGGGtttgt                                                                                                                    >  5:1462710/1‑149 (MQ=11)
                                      acatGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATCCACAAAAGAGAGCGGCTCTTCGCTCAATTTCACATTTTGTAAGTAAAAACCCGATTCGGCGTGgtt‑tt                                                                                                                   >  5:132389/4‑150 (MQ=11)
                                      aCCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGtttgtc                                                                                                                   >  3:1526622/1‑148 (MQ=25)
                                      aCCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAACCCGCATCGGCGGGttt‑ta                                                                                                                   >  6:715703/1‑149 (MQ=255)
                                         tGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCGGGTTT‑GAACtt                                                                                                               >  4:1198646/1‑151 (MQ=11)
                                           gTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGtttgagatttt                                                                                                              >  1:1087868/1‑143 (MQ=21)
                                           gTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGGTGCGGCGGGtttgtgattgt                                                                                                              >  6:30643/1‑143 (MQ=11)
                                             ttACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTT‑TTACTTTTgg                                                                                                           >  1:427710/1‑151 (MQ=25)
                                              tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGGCGGGTTT‑GTGCTTGTggc                                                                                                          >  4:1519049/1‑150 (MQ=9)
                                              tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACACGATACGGCGAGTTt‑ataatagtgg                                                                                                           >  4:203233/1‑143 (MQ=11)
                                              tACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGGCGGGTTT‑ATACtttttg                                                                                                           >  8:1174256/1‑148 (MQ=11)
                                               aCGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACCGATACGGCGGGTTT‑GAAATTTTgg                                                                                                           >  8:437699/1‑149 (MQ=11)
                                                    accaCCGGCGTCGGCGGGTTTTTGGGTTTGCCGGGGCACCCAGATGAAGGCCTCTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCa                                                                                                    <  3:251985/148‑1 (MQ=11)
                                                     acaCCCTCTGCGGCGGGTTTTGGCGTTTGCCGGGGCCCCCCCATGCAGGACTCGCCACCACACTATACCCAAAAGAAACCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAg                                                                                                   <  7:129904/149‑1 (MQ=11)
                                                     aaaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGTCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTGCGGCGGGTTT‑GTCCTTTTGGAgggcc                                                                                                     >  8:1533828/1‑147 (MQ=11)
                                                     aaaCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGTGGc                                                                                                     >  6:1401196/1‑149 (MQ=25)
                                                       aCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTACGGCGGGTTT‑GTAATTTTTGAGTGGCatt                                                                                                  >  2:904289/1‑148 (MQ=11)
                                                       aCCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGGCGGGTTT‑AAAATTTTGGAGTGGCata                                                                                                  >  6:1577376/1‑148 (MQ=11)
                                                         ccGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGTGGCAGaaat                                                                                               >  7:1003417/1‑150 (MQ=25)
                                                         ccGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAACCCGATTCGGCGGGTTT‑ATACTTTTTGAGTGgaagaatg                                                                                               >  4:507644/1‑149 (MQ=11)
                                                         ccGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGTTGCGGCGGGTTTGTTATTTTTgagtgggagtaat                                                                                                >  2:1412618/1‑139 (MQ=11)
                                                           gCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGCTACGGCGGGTTT‑TTCATTTTGGAGTGGGAGtaata                                                                                              >  5:1283222/1‑148 (MQ=11)
                                                            cTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGATTCGGCGGGTTT‑ATTATTGTTGAGGGGCatttata                                                                                              >  5:527910/1‑143 (MQ=11)
                                                            cTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTTCGGCGGGTTT‑TACATTTTTGAGTGCCAGaaat                                                                                               >  5:35273/1‑147 (MQ=11)
                                                              tCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAACCCGATACGGCGGGTTT‑AAACTTTTTGAgtgcgagatattcgtg                                                                                          >  2:960282/1‑136 (MQ=11)
                                                                ggcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCGGGTTT‑ATAATTTTTGAGTGCCATaaataaataa                                                                                         >  6:731017/1‑143 (MQ=11)
                                                                  cggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTGCGGCGGGTTT‑GTAATTTTGGAGTGCCAGATATAGAAATG‑Ac                                                                                     >  5:410270/1‑151 (MQ=11)
                                                                  cggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACAGCTTCGGCGGGTTT‑AAAATTTTGGAGGGCGAGaaatagcaaaccc                                                                                      >  1:657231/1‑141 (MQ=11)
                                                                   gggTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGTTTTACGTAAAAACCCGGTACGGCGGGTTT‑TTCATTTTGGAGTGGCatttataactatccc                                                                                      >  1:1304128/1‑136 (MQ=11)
                                                                      tttttGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTT‑GTCCTTTTGGAGTGGGAGAAAGaggatgccctggc                                                                                  >  1:595696/1‑142 (MQ=11)
                                                                       ttttGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGGTGCGGCGGGTTT‑TAGCTTTTGGAGTGGGAGAAagaagattcccattcc                                                                                 >  7:372832/1‑141 (MQ=11)
                                                                               ttGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGGCGGGTTT‑ATAATTTTTGAGTGCCAGAAAGAAGAAAG‑ACTATcgacgagaa                                                                         >  8:353166/1‑147 (MQ=11)
                                                                                   aGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAACCCGCTGCGGCGGGTTT‑TTACTTTTTGAGTGGCAGATATATGAGAG‑ACTGTCGACGACACtaga                                                                     >  8:571037/1‑148 (MQ=11)
                                                                                      ggCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTT‑GTGCTTGTGGAGGGGGAGAAAGAGGTCTG‑GCAGTCCGCGACACtggacca                                                                  >  7:472944/1‑144 (MQ=11)
                                                                                         aGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGTGGCAGAAAGATGAATG‑ACAGTCCACGACACTATACACaaa                                                               >  8:614842/1‑150 (MQ=255)
                                                                                                ccacccactccccACGACCCAAACCCCAAAACAACCCGCCTAAGCGGTCAGTTTAACCTTTTTAAAGTAAAAACCCGCTTCGGCGTTTTT‑TTTCTTTTTGAGGCGCACAAACCTGAACC‑CATCCCAACTACACTATACCCAAAAGAAAGCg                                                       <  3:804837/140‑1 (MQ=2)
                                                                                                     gattttttttttcacTTTACACTAAAGAAACGGGCTTTTTGTTACGTTTCAGGTGTTTTATGTAAAACGCGGCTTCGGCGGGTTT‑TTTTTTTTGTTGGGGGTTAAAGTTGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCtt                                                   <  6:1446469/138‑1 (MQ=2)
                                                                                                         gTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTT‑TTCATTTTGGAGTGCCAGAAAGAAGAAAC‑ACTGTCCACGACACGATAGCCAAAAGACAACCCCTTATCgg                                              >  8:259505/1‑151 (MQ=11)
                                                                                                         gTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATACGGCGGGTTT‑GAAATTTTGGAGGGGGAGATAGAACAAAC‑ACTTTCGCCGACACTAGAAACTCATGAAAGCGGATTATCgg                                              >  2:671068/1‑151 (MQ=2)
                                                                                                             aCGACACTATACCTAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTGCGGCGGGTTT‑TTTCTTTTTGAGTGGCATAAAGAAGAAAC‑ACAGTCCCCGACACTATACCCAAAAGAAAGCACCTTATCGCTCat                                          >  1:1408971/1‑150 (MQ=11)
                                                                                                             aCGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGATTCGGCGGGTTT‑TTACTTTTTGAGGGGCAGAAAGAAGAATG‑ACTGTCGACGACACTAGAAACAAAAGAAAGcctcagatcggacat                                          >  4:235685/1‑137 (MQ=11)
                                                                                                               gACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACGATACGCAAAAGAAAGCGGCATAACGGTCAGt                                         >  4:133243/1‑150 (MQ=255)
                                                                                                               gACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑ATACTTTTTGAGGGCCATAAATAGTAAAC‑ACAGTCGACGACACTCTAAGAAAAAGAATACAGATTAACGGTCAGtt                                        >  3:892677/1‑151 (MQ=2)
                                                                                                                acacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTGCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGAAGAAAC‑ACTGTCCCCGACACGATACTCACAAGAAAGCCCATGATCGGTCAGtat                                       >  3:1421756/1‑149 (MQ=11)
                                                                                                                 cacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTTGAGTGGCAGAAAGATGATTG‑CCTGTCCCCGAGAATATACCCAAAAGAAAGCTGCAGATCGGTCATtttt                                      >  1:1050558/1‑151 (MQ=11)
                                                                                                                     ataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTGCGGCGGGTTT‑ATACTTTTGGAGTGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCAAAAAGAAAGCGGCTTATCGGTAAGTTTTAACt                                  >  3:1188616/1‑151 (MQ=11)
                                                                                                                     ataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTTGAGTGGCAGAAAGATGAATC‑CCTGTCGCCGACACTAGACCAAAAAGAAACCGGCTTATCGGTCAGTTTTACCt                                  >  1:640185/1‑151 (MQ=11)
                                                                                                                     ataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGGAGAAAGATGAATG‑ACTGTCGACGACACTATACGCAAAAGAAAGCGGCTTATCGCTCAGTGTTACCt                                  >  8:578108/1‑151 (MQ=255)
                                                                                                                        cccAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑GTACTTTTTGAGGGGCAGAAATAAGAAGC‑ACAGTCCACGACACTATCCAAAAAAGAAAGCGCATTATCGGACAGTATTACCTGAt                               >  4:1464534/1‑151 (MQ=11)
                                                                                                                        cactaaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAggg                                                                                                       <  2:629092/76‑1 (MQ=255)
                                                                                                                         ccAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTAATTTTTGAGTGGCAGATAGATGAAAG‑ACTGTCGACGACACAATACGCAAAAGAAAGCGGATTATCGGTGAGTTTTAGCTGATg                              >  3:588528/1‑151 (MQ=14)
                                                                                                                          cAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATC‑ACTGTCCACGACACGAGACCCTACAGCCAGCGGATTAACGGTCAGTTTTTCCTGAGGt                             >  5:1524287/1‑151 (MQ=11)
                                                                                                                           aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGt                             >  5:1009453/1‑150 (MQ=39)
                                                                                                                            aaagaaagCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGTGGCAGAAAGATGAATG‑ACTGTCCACGACACTATAACCAAAAGAAAGCGGCTTATCGGTCAGTATTACATGATGTAc                           >  4:1319287/1‑151 (MQ=255)
                                                                                                                            aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑GCTGTCGCCGACACGATAGCCAAAAGACAGCGGCTGATCGGTCAGTGTTACCTGATCTa                            >  2:1100673/1‑150 (MQ=11)
                                                                                                                            aaagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTAc                           >  1:1524166/1‑151 (MQ=40)
                                                                                                                               gaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAGTG‑CCTGTCCCCGACACTCTACCCAAAAGAAAGCCCCTTATCGGTCAGTTTTACCTGATGTACGt                         >  5:107987/1‑150 (MQ=14)
                                                                                                                                 aagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑GTACTTTTGGAGGGCCAGATAGATGAAAG‑AGTGTCCACGACACAATCCGCAAAAGAAAGCCGATTAACGGTCATTTTTAATTGAGGTACGaaat                      >  7:1491614/1‑151 (MQ=11)
                                                                                                                                  agCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGtaat                      >  7:1220791/1‑150 (MQ=255)
                                                                                                                                   gCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGttata                     >  7:759386/1‑150 (MQ=255)
                                                                                                                                     gggtccgcGGTCCGTTTCACCGGTTATACGTAAAACCCCGCTGCGGGGTTTTT‑TTTCTTTTGGAGGGCAAGAAAGA‑GTATGTACGCTCCACGACCCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                  <  7:697387/144‑1 (MQ=9)
                                                                                                                                     ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                  >  6:371631/1‑151 (MQ=255)
                                                                                                                                       cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTTCTTTTGGAGGGGCAGAAAGatga                                                                                           <  1:528103/77‑1 (MQ=18)
                                                                                                                                       cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTTTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                >  8:1282837/1‑151 (MQ=255)
                                                                                                                                       cTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCg                >  8:1275894/1‑151 (MQ=255)
                                                                                                                                        ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACATGATGTACGTAATAAACCGt               >  8:315390/1‑151 (MQ=255)
                                                                                                                                        ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTGTTGGAGGGGCAGCAAGCTGAATG‑ACTGGCACCGACACTATACCCAAAAGAAAGAGGCTGATCGGTCAGTGTTACCGGATGTACGTAATAAACCGt               >  3:1497560/1‑151 (MQ=14)
                                                                                                                                        ttATCGGTCAGTTTCACCTATTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACCCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt               >  2:339256/1‑151 (MQ=255)
                                                                                                                                         tctctgccgttttCACCTGTTTTACTTAAAAACCCGCTTCGGCGGGTTT‑TTTCTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAGCCGt               <  1:1232248/140‑1 (MQ=14)
                                                                                                                                         tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGtt              >  4:1077062/1‑151 (MQ=255)
                                                                                                                                         tATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTGTTACCTGATGTACGTAATAAACCGtt              >  2:1464232/1‑151 (MQ=255)
                                                                                                                                           tCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTcc            >  4:1553856/1‑151 (MQ=255)
                                                                                                                                            cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg           >  2:592987/1‑151 (MQ=255)
                                                                                                                                            cGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg           >  2:117431/1‑151 (MQ=255)
                                                                                                                                             ggTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcgg          >  5:560009/1‑151 (MQ=255)
                                                                                                                                              gTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggc         >  3:1237242/1‑151 (MQ=255)
                                                                                                                                               tCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcg        >  4:851257/1‑151 (MQ=255)
                                                                                                                                                cAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg       >  5:444827/1‑151 (MQ=255)
                                                                                                                                                atgtatCACCTGATTTCCTTAAATCCACGCATTGGGGGGTTT‑TTTGCTTAGGTGGGCGCGAAAGATGAAGG‑CCTCGACACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg       <  7:1027599/146‑1 (MQ=9)
                                                                                                                                                 attttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑AATGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTAAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg      >  8:779323/3‑151 (MQ=255)
                                                                                                                                                    ttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt   >  8:831221/1‑151 (MQ=255)
                                                                                                                                                    ttCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt   >  4:1006817/1‑151 (MQ=255)
                                                                                                                                                      cACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  6:1093056/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
CGCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCGCTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGGCGGGTTT‑TTACTTTTGGAGGGGCAGAAAGATGAATG‑ACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613620‑613919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑