Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,1690T.40.5% 157.3 / 116.3 80intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (1/43);  new base . (2/28);  total (3/77)
Fisher's exact test for biased strand distribution p-value = 5.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGC  >  DH1/936019‑936306
                                                                                                                                                      |                                                                                                                                           
gAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                             <  3:597859/151‑1 (MQ=255)
gAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                             <  6:691565/151‑1 (MQ=255)
cacagcagcgagtttAAGGCAGACCAGAAACAACAACAGATCCTTTAGCGTGAAAAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCt                                                                                                                                             <  3:1218641/139‑1 (MQ=11)
 aaTGGCGCTTTTTTAAGGGACACCAGCAACAAAAAAACCCCCGTTAGGGTGATCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                            <  3:890369/151‑1 (MQ=255)
  aTGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                           <  3:598536/151‑1 (MQ=255)
   ttgttcTTTTTTATTGGAGACCAGTAACAAATAAACACCTTTTAGGTTGTTAAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttt                                                                                                                                           <  2:380456/145‑1 (MQ=17)
    ggctcgtgtttATGGGAGACCAGCACCCACAAAACCCGCTTCAGGGTGATCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCttta                                                                                                                                          <  7:898963/142‑2 (MQ=14)
    ggagcTTTTTTATGGGAGACCAGAAACAAAAACACACCCGTTAGGGTGTTCAATAATTGGTTTCGTGGGCCGGATTTGAACCGACTACATTCGTGTTATGAGCCAGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                          <  2:368309/147‑1 (MQ=21)
    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCATTAATTGGGTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTTCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                          <  1:951059/150‑1 (MQ=255)
    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGTGGCCTGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttt                                                                                                                                          <  2:814700/150‑1 (MQ=255)
    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                         <  5:1408321/151‑1 (MQ=255)
    ggCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTa                                                                                                                                         <  4:1106171/151‑1 (MQ=255)
     gcgcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTAc                                                                                                                                        <  8:201227/151‑1 (MQ=255)
      ggcTTTGTTATGGGAGACCAGAACAAAAAACACAGCCGTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttactt                                                                                                                                       <  6:70843/150‑5 (MQ=17)
       gcTTTTTTATGGGAGACCAGAAACAAAAAAAGACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTtc                                                                                                                                     <  2:1331412/151‑1 (MQ=255)
       gcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttacat                                                                                                                                       >  8:332878/1‑146 (MQ=255)
       gcTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACtt                                                                                                                                      <  4:743347/151‑1 (MQ=255)
       gcTTTTGTATTGGAGACCTGAAACAAAAACACACCGTTTATGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTtc                                                                                                                                     <  3:630924/151‑1 (MQ=14)
        cgtttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtc                                                                                                                                     <  4:935885/149‑1 (MQ=255)
         gtttttATGGGAGACCAGATAAAAAAAAACAGCAGTATTGGTGTTCTATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTtcat                                                                                                                                   <  7:860275/150‑1 (MQ=14)
          ttttttttGGAGACCAGTTAAAATAAAACATCCGTTATGTTGTTAAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTtc                                                                                                                                     <  2:855590/148‑1 (MQ=11)
          ttttttTGGGAGACCAGAAAAAAAAAAACACCCTTTAGGTTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTtcat                                                                                                                                   <  6:1167084/150‑1 (MQ=17)
          tttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtc                                                                                                                                     <  7:1330998/149‑1 (MQ=255)
          tttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtc                                                                                                                                     <  3:1536191/149‑1 (MQ=255)
          tttttATGGGAGACCAGAAAAAAATAAAGCCCGGTGTGGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTtcat                                                                                                                                   <  3:75638/150‑1 (MQ=14)
           ttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcat                                                                                                                                   <  1:772420/150‑1 (MQ=255)
           ttttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcat                                                                                                                                   <  5:461378/150‑1 (MQ=255)
            ttttTGGGAGACCAGAAACAAAAACACACCCGTGAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTACTCCACCCCGCGTCACCGTACTGCTTTACTtcatca                                                                                                                                <  5:744632/150‑1 (MQ=17)
            tttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatc                                                                                                                                 <  7:1455088/150‑1 (MQ=255)
            tttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatc                                                                                                                                 <  8:954051/150‑1 (MQ=255)
             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtttacgtcatgaa                                                                                                                                 >  6:812737/1‑140 (MQ=255)
             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                                <  1:1079137/150‑1 (MQ=255)
             ttATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGCTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTtcatca                                                                                                                                <  6:836867/150‑1 (MQ=255)
             ttATGGGAGACCACAAACACTAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGGTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCaa                                                                                                                               <  5:1175909/150‑1 (MQ=17)
             gtATGGGAGCCCACAAACAAAAACTCCCCCGAGAGGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCaa                                                                                                                               <  1:942285/149‑1 (MQ=14)
              tATGGGAGACCAGAAACAAATAAACACCCGTTAGTGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACGTACGACCTTCGTGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGGCACCGTACTGCTTTTACTTCATCaa                                                                                                                               <  4:502836/150‑1 (MQ=18)
              tATGGGAGACCAGAAACAAAAAAACTCGCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACTACCTTCGGGTTATGAGCCCGACGAGCTACCATGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaa                                                                                                                               <  6:1301638/150‑1 (MQ=255)
               aggggAGACCAGCACCAAAAACAGCCCGGTGAGGGTGTTCAATCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGGTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAt                                                                                                                            <  7:1235959/148‑1 (MQ=14)
               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCaaa                                                                                                                              <  3:1166737/150‑1 (MQ=255)
               aTGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAt                                                                                                                            <  8:291241/151‑1 (MQ=255)
                tGGGAGACCCGAAACAAATAATGACCCTTTAGGGTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAtt                                                                                                                           <  6:1040544/150‑1 (MQ=14)
                 tggAGCCCACAAACAAAAAAAGCCCCTTTAGGGTGTTAAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttt                                                                                                                          <  5:1168920/149‑1 (MQ=14)
                 gggAGACCAGAAACAAAAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttt                                                                                                                          <  7:677225/150‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAttt                                                                                                                          <  6:993549/151‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAttt                                                                                                                          <  4:782595/151‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAttt                                                                                                                          <  4:145434/151‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAtt                                                                                                                           <  7:90588/150‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAAtt                                                                                                                           <  5:54661/150‑1 (MQ=255)
                 gggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAtttt                                                                                                                         <  1:648498/151‑2 (MQ=255)
                 gggACACCAGAAACAAAAAAACACCCGTGAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttt                                                                                                                          <  5:810351/150‑1 (MQ=255)
                 ggcacacctcaaaaaaaaaaACACCCTTAAGGTTGATCTATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAtttt                                                                                                                         <  1:35890/141‑2 (MQ=11)
                  ggagccctttaaCAATAAAACACTCTTTTTGTTGTTAAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAtttta                                                                                                                        <  2:1150633/141‑3 (MQ=1)
                  ggAGCCCACCAAAAAAAACACACGCTTGAGCGTGTACAATAATTGGGTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAtttta                                                                                                                        <  3:277466/151‑3 (MQ=11)
                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTg                                                                                                                         <  5:596413/151‑1 (MQ=255)
                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTg                                                                                                                         <  3:1376038/151‑1 (MQ=255)
                  ggAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTg                                                                                                                         <  1:220820/151‑1 (MQ=255)
                   gagaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGa                                                                                                                        <  8:607723/151‑1 (MQ=255)
                   gagaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGa                                                                                                                        <  1:391088/151‑1 (MQ=255)
                    agaCCAGAAACAAAAAAACACCCGTGATGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaat                                                                                                                      <  3:863886/151‑5 (MQ=255)
                    acccccgcaccAAAAAAACACCCTTGAGCGTGTTCATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaat                                                                                                                      <  7:331026/141‑5 (MQ=11)
                     gaCCTGAACCAATAAAACACCGGTTTGGGTGATCAATTATTGGTTCCGGGGGCCGGATTTGAAGCGACGACGTTCTGGTTATCAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTAGTGGTTTACTTCATCAAAttttaat                                                                                                                      <  1:1319805/150‑5 (MQ=11)
                     gaCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGAtt                                                                                                                      <  3:628836/151‑1 (MQ=255)
                      aCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCCGTACTGCtttacgtcctgca                                                                                                                                 >  3:174418/1‑131 (MQ=255)
                      aCCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTg                                                                                                                     <  3:555546/151‑1 (MQ=255)
                      aCCAGAAAAAAAAAAACACTGTTTAGGTTGTTAAATTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaattg                                                                                                                    <  4:142450/151‑7 (MQ=11)
                       ccAGAAACAAAAAAACCCCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaattgg                                                                                                                   <  3:584533/151‑8 (MQ=255)
                       ccAGAAACAAAAAAACAGCCTTTTGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAttttaattgg                                                                                                                   <  6:1193987/151‑8 (MQ=14)
                       ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTgg                                                                                                                    <  1:1154163/151‑1 (MQ=255)
                       ccAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTgg                                                                                                                    <  3:896322/151‑1 (MQ=255)
                        cAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACACCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGt                                                                                                                   <  7:1473359/151‑1 (MQ=255)
                         agcaacaacAAAACCCCCTTGAGGGTGTTCATTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGtt                                                                                                                  <  7:734902/148‑1 (MQ=11)
                         agatccAAATAAACACCTTTTTGGGTGTTAATTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGtt                                                                                                                  <  4:1477325/146‑1 (MQ=11)
                         acaaaCAAAAACACCCCCGACAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGtt                                                                                                                  <  1:1234039/149‑1 (MQ=11)
                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTtca                                                                                                                                    >  8:1259875/1‑134 (MQ=255)
                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGtt                                                                                                                  <  6:1173447/151‑1 (MQ=255)
                         aGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTAATTAATAAAAATTTAATTGGtt                                                                                                                  >  8:738985/1‑151 (MQ=14)
                          gAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTg                                                                                                                 <  6:1469983/151‑1 (MQ=255)
                          gAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTg                                                                                                                 <  5:1371392/151‑1 (MQ=255)
                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGc                                                                                                                <  8:161294/151‑1 (MQ=255)
                           aaaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGc                                                                                                                <  5:325739/151‑1 (MQ=255)
                            aaCAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCg                                                                                                               <  2:1362103/151‑1 (MQ=255)
                              cAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCtt                                                                                                                                            >  6:1427278/1‑122 (MQ=255)
                              cAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCggg                                                                                                             <  1:527299/151‑1 (MQ=255)
                               aaaaaaaCACCCGTTAGGGTGTTGAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCgggg                                                                                                            <  8:563838/151‑1 (MQ=255)
                               aaaaaaaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGACCCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCgggg                                                                                                            <  7:820676/151‑1 (MQ=255)
                                 aaacaCACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGc                                                                                                          <  1:1370244/151‑1 (MQ=255)
                                  aaaaCACCCGTTAGGGTGTTCAATAATTGTTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTGTTACTTCATCAAATTTGATTGGTTGCGGGGGcc                                                                                                         <  3:752159/151‑1 (MQ=255)
                                         acGTTCGGGTGTTAAATATTTTTTTGCGGTGGCCGGACGCGCCCCGCCGACGTTCGCTTTATCAGCCCGACCAGCGACGAGTTTCCTCGACACCCCCTAACACTACTCCCTTTACTTCCTCAAAATTCATTGGTTGCGGGGGCCGGATTTg                                                                                                  <  3:1374269/150‑1 (MQ=255)
                                          cgggaGGGTGTTAAATAAATGGTTGCGCTGGCCGGAATGGACCCGCAGCCGTTGGTGTTAAGCGCCCGACGAGCTACCATGCTCCGGGACCCCCCGTCAACGTACTGCGTTTACATCCTCATAATTGATTGGTTGCGGGGGCCGGATTTGa                                                                                                 <  1:751934/147‑1 (MQ=9)
                                             aggggTGTTCAATCATTGGTGGCGCTGGCCGGACGCGCGCCGTCGGCCTTCGGGATATCAGCCCGACGAGCGCCCAGGCTGCGGGACCCCGCGTCACCCAACTGCCTGTACATCATGTAAATTGATTGGTTGCGGGGGCCGGATTTGAAc                                                                                               <  7:105224/148‑1 (MQ=9)
                                              ggggTGTTCAATCATTGGTGGCGCTGGCCGGACGCGCGCCCCCGCGCTTGGCGTTATGCGCCCGCCGAGCTACGAGGCTGCGGGAGCCCCCGTCACCGTACTGCTTCTACTTGCTCAAAATTCATTGGTTGCGGGGGCCGGATTTGAACcg                                                                                             <  7:768101/150‑1 (MQ=9)
                                                  tgtTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAtt                                                                                                                           <  2:971685/116‑1 (MQ=18)
                                                              ggTTGCGGGGGCCGGAGTTTATCCCACCCCCTTCGGTTAATGCGCCCGACGTGGTACCAGTCTGCTCCACCCCGCTACACCGAACTGCGTTTACTTCATCATATATGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                             <  7:1114515/151‑1 (MQ=11)
                                                                                                                          tcttcgcgaCCCCACTTCACCGTACTCCGTCGACTGCCTCATATTTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTtcatca                  <  5:971152/142‑1 (MQ=14)
                                                                                                                                           cACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGTAATCCATGACTTCATCACATTTTAATTCGTTGc  >  4:775942/1‑149 (MQ=17)
                                                                                                                                                      |                                                                                                                                           
GAATGGCGCTTTTTTATGGGAGACCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGC  >  DH1/936019‑936306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: