Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2930T.32.0% 229.6 / 120.2 99intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (61/5);  new base . (29/2);  total (92/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.29e-02

CACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAT  >  DH1/936158‑936442
                                                                                                                                        |                                                                                                                                                     
cACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGTAATCCATGACTTCATCACATTTTAATTCGTTGc                                                                                                                                          >  4:775942/1‑149 (MQ=17)
                                                         gacgacCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCCGGACTGGGTTTCTTCTGCAATGTGGTATTGGTTGCGGCGGCCGGATTTGACCCGCCGGCCTTAGGGTTCTGATCGAGCCGAGCGAACAGgctgc                                                                               >  7:952632/1‑151 (MQ=2)
                                                               cTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGTGCTAGTCTTCTGCGAATTTTAATTGGTTGCGGGGTCGGGTTATGACCCGCCGCCCTTCGGGTTCTTAGCCCGCCGAGCGACCAGGCTGCAcccc                                                                          >  7:1014158/1‑148 (MQ=2)
                                                                ttCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACGTCATGAAATTTTa                                                                                                                                                   >  5:909856/1‑76 (MQ=255)
                                                                                       tACCAGGCTGCTCCACCCCGCGTCAGGGTAGTGGTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCGGGATTTGAACCGACGACCTTAGGGTTATGATCCAGAAGAGCTACCAGGCTGCTCCCCCCCGAGTCAGTGGATTCGCACtat                                                  >  3:1504848/1‑150 (MQ=17)
                                                                                              ctgctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCTGATTTGAACCGACGCCCTTCGGGTTATGAGCCAGACGAGCTACCAGGCTGCTCCACCCCGCTTCGGTTGATGGGCACTATACTCCGCt                                          >  1:461826/1‑151 (MQ=17)
                                                                                              ctgctCCACCCCGCGTCACCGTACTGCGTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACTATACTCCGcg                                          >  7:1056501/1‑150 (MQ=255)
                                                                                              ctgctCCACCCCGCGTCACAGTAATCCATTAATTCAGCAAAGTATAATTCATTCCGCAGGACGGATTTGAAACGACGACCTTCGGGTTATGAGCCAGACTAGATACCAGGCTGCTCCACCCAGCGTAAGAAGATGCGCACTATACTCCGCt                                          >  6:1378821/1‑151 (MQ=12)
                                                                                               tgctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACATTAGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTc                                         >  7:1166016/1‑151 (MQ=255)
                                                                                                  tCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                                      >  3:658780/1‑151 (MQ=255)
                                                                                                   ccACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGCGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGt                                     >  5:61396/1‑151 (MQ=255)
                                                                                                    cACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                    >  7:127704/1‑151 (MQ=255)
                                                                                                     aCCCCGCGTCACCGTACTGCTTTTACTTAATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGAGTCGCAGTATACACCCCTCTCGttt                                   >  3:1096078/1‑151 (MQ=11)
                                                                                                     aCCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                                   >  2:750206/1‑151 (MQ=255)
                                                                                                     aCCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                                   >  5:996259/1‑151 (MQ=255)
                                                                                                     aCCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATAATCCGCTCGCGttt                                   >  6:575157/1‑151 (MQ=255)
                                                                                                       cccGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGATCGCGttttg                                 >  1:480857/1‑150 (MQ=14)
                                                                                                       cccGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAAGCCGACTATAcatccaaagagttttc                                 >  7:500767/1‑136 (MQ=11)
                                                                                                       cccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                 >  1:1270177/1‑151 (MQ=255)
                                                                                                        ccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                 >  2:434263/1‑150 (MQ=255)
                                                                                                         cgcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                               >  7:673001/1‑151 (MQ=255)
                                                                                                           cgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGGGCAGTATACTACAATCGTGTTTTcgttg                             >  5:719431/1‑147 (MQ=11)
                                                                                                           cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                             >  3:936101/1‑151 (MQ=255)
                                                                                                             tatctgtacttcttttACTTATTAAATTTTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACGAGGCTGCTCCACCGCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                            <  8:873305/137‑1 (MQ=18)
                                                                                                             tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTACGCTAGAGTTttcattca                            >  8:1201263/1‑150 (MQ=14)
                                                                                                             tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                            >  5:1541545/1‑150 (MQ=255)
                                                                                                             tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                            >  6:1149148/1‑150 (MQ=255)
                                                                                                              cACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGTCGACCTTCGCGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                          <  5:1373289/151‑1 (MQ=255)
                                                                                                               aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAATATACACCAATCGCTTATTCATGCAAcc                         >  3:1414503/1‑151 (MQ=11)
                                                                                                               aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                         >  4:842146/1‑151 (MQ=255)
                                                                                                               aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                         >  7:946638/1‑151 (MQ=255)
                                                                                                                ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGGAATATACTACGCTCGAGATTTCATGCAACCt                        >  4:971276/1‑151 (MQ=11)
                                                                                                                ccGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGCGCCCGACGAGCTACCAGCCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                        >  2:1189175/1‑151 (MQ=255)
                                                                                                                ccGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                        >  6:281523/1‑151 (MQ=255)
                                                                                                                 cGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGTCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGATCGCGTTTTCATGCACCCtt                       >  1:301676/1‑151 (MQ=14)
                                                                                                                 cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                       >  2:810800/1‑151 (MQ=255)
                                                                                                                  gTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAGTGGGAGACTACTCCGCTCGCTTTTTcgttccgggggg                      >  6:689598/1‑140 (MQ=11)
                                                                                                                  gTACTGCTTTACTTCCACAAATTTTTATTGGTTGCGGCGGCCGGATTTGAACCGACGACCTTCGGTTTATGAGCCCGACGAGCTAGCAGGCTTCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttt                      <  7:375072/151‑1 (MQ=21)
                                                                                                                   tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCaa                           <  1:1491998/145‑1 (MQ=255)
                                                                                                                   tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTAGCGTTTTCATGCaaagatcc                     >  2:1508779/1‑145 (MQ=14)
                                                                                                                   tACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACTATACTCCCCTCGAGTTTTCATGCAACCtttt                     >  2:876605/1‑151 (MQ=14)
                                                                                                                   tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                     >  1:109663/1‑151 (MQ=255)
                                                                                                                   tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                     >  7:1499944/1‑151 (MQ=255)
                                                                                                                   tACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                     >  4:1475609/1‑151 (MQ=255)
                                                                                                                    aCTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAAGCGGTCTATTCTCCGATCGAGttttgtttcacccgtttt                    >  3:1040012/1‑137 (MQ=11)
                                                                                                                    aCTGCTTTACTTCATCAAATTTAAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCTGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                    <  8:1062321/151‑1 (MQ=255)
                                                                                                                     ctgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGAGTATACTCCTCAAGAGTTTTCATGCATCCttgatt                   >  5:460265/6‑147 (MQ=11)
                                                                                                                     ctgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATTCGCTCTATACTACGCTCGAGTTTTCCTGCAACCtttttt                   >  1:361968/6‑151 (MQ=14)
                                                                                                                     cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                   >  2:916878/1‑151 (MQ=255)
                                                                                                                      tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                  >  8:764472/1‑151 (MQ=255)
                                                                                                                      tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                  >  7:124214/1‑151 (MQ=255)
                                                                                                                      tGCTTTACTTCATCAAATTTTAATTGGTTGCGCGGCCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGCTGCGCACTATACTCCGCTCGCGGTTTCATGCAACCtgttttt                  >  6:97702/1‑151 (MQ=255)
                                                                                                                       gCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                 >  4:1380917/1‑151 (MQ=255)
                                                                                                                       gCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                 >  1:869463/1‑151 (MQ=255)
                                                                                                                       gCTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTa                <  5:1306495/151‑1 (MQ=255)
                                                                                                                        cttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCtttttttt                 >  1:822043/3‑150 (MQ=255)
                                                                                                                        cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                 >  3:1025057/1‑150 (MQ=255)
                                                                                                                        cTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                 >  2:1137808/1‑150 (MQ=255)
                                                                                                                          tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCCCTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAc               >  8:1286783/1‑151 (MQ=255)
                                                                                                                          tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAc               >  8:761683/1‑151 (MQ=255)
                                                                                                                          tttACATCATCAAATTTTAATTGGTAGCGGGGGCCGGATATGAACCGACGACCTTCGGGTTATCAGCCCGACGAGCTACCAGGCTGCTCCACCACGCGTCAGTGGAAGCGCACTATAATACGCTCGCGTATTCATGCAACCTATTTTTTa                >  6:912534/1‑150 (MQ=18)
                                                                                                                           ttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTACGATAGCGTTTTCATTCAACCTTATTTTTa                >  6:1312007/1‑148 (MQ=17)
                                                                                                                            tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc             >  4:752069/1‑151 (MQ=255)
                                                                                                                            tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGCACTATACTCCTCTCTATTTTTCATTCAGCGTTTTTTTTTccc             >  7:1440116/1‑150 (MQ=14)
                                                                                                                            tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAGGGGGTGTATACTCCGCTGGGGTTTTCGTGCAGCGGTGTTTTTaggcc            >  7:232116/1‑147 (MQ=1)
                                                                                                                            tACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAATCCGTCTATACACCCCTTGCTTTTTCATTCAACGTTTATTTTaaca             >  1:74197/1‑147 (MQ=1)
                                                                                                                               ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCgc          >  5:382682/1‑150 (MQ=255)
                                                                                                                               ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg           >  8:1180991/1‑150 (MQ=255)
                                                                                                                               ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg           >  1:1423733/1‑150 (MQ=255)
                                                                                                                               ttCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAGTATACTCCGCTCTCGTTTTCAt                               >  1:395748/1‑129 (MQ=17)
                                                                                                                               ttCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCGGAGTATACTCCGATCTGTTTTTCATTCACGCTGTTTTTTACCCCgct         >  7:1180913/1‑149 (MQ=14)
                                                                                                                               ttCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACTGGATTCCAAAAATACTACAATCTATTTTTCATTCAACCTTTTTTTTaacaaga          >  2:589688/1‑144 (MQ=11)
                                                                                                                                tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg           >  4:853064/1‑149 (MQ=255)
                                                                                                                                tcatcaAATTTGAATGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGATATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGACCCAGGAAGCGCAGAATACTACACAGGAGATTTCCTGCAGCCTGATTATTACCCCGaac        >  4:1114900/1‑149 (MQ=2)
                                                                                                                                tcatcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAGTATACTCCGCTCGCGATTTCCTTGCACCGTTGTTTTAGCCCCAt         >  5:992320/1‑150 (MQ=14)
                                                                                                                                tcatcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGGACTATACTACGATCGAGTTTTCATGCAACCTTATTTTTAACCCCATc        >  4:158620/1‑151 (MQ=17)
                                                                                                                                tcatcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCGGACTATACTCCGCTCGAGTTTTCATGCACCCTTTGTTTTACCCCGATc        >  5:1045065/1‑151 (MQ=18)
                                                                                                                                tcatcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGAGTATGCGAAAAATACAACGCTAGATAATTCATTCAGCCTGTATTTTAACACGATc        >  4:955575/1‑151 (MQ=11)
                                                                                                                                tcatcaAATTTGATTGGTTGCGGGGGCCGCATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGAACGCCGAGTATACAACCATCTAGTTTTCATGCAGCCtgaatattatcccgct         >  8:998904/1‑135 (MQ=2)
                                                                                                                                 taataaaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt         <  8:1187204/146‑1 (MQ=255)
                                                                                                                                 catcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATGCGCACTATACTCCGCTCGATTTTTCATGCAACCTGTTTTTTAACCCGATc        >  6:358865/1‑150 (MQ=17)
                                                                                                                                  atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATc        >  3:192033/1‑150 (MQ=255)
                                                                                                                                  atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTACGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGGTTTCATGCAACCTTTTTTTTACCCCGATc        >  8:154615/1‑150 (MQ=255)
                                                                                                                                  atcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATGCGAAAAATACTCCGCTAGATTATTCATGCAACCTTTATTTTAGCCACATCAc      >  8:793023/1‑151 (MQ=14)
                                                                                                                                   tcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGGTTTCATGCAACCTTTTTTTTACCCCGATCAc      >  4:1378858/1‑151 (MQ=255)
                                                                                                                                   tcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc      >  3:87021/1‑151 (MQ=255)
                                                                                                                                   tcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc      >  2:279944/1‑151 (MQ=255)
                                                                                                                                    caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg     >  3:59761/1‑151 (MQ=255)
                                                                                                                                    caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg     >  2:652486/1‑151 (MQ=255)
                                                                                                                                     aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg    >  8:1082396/1‑151 (MQ=255)
                                                                                                                                     aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg    >  3:1057663/1‑151 (MQ=255)
                                                                                                                                      aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg    >  1:1256683/1‑150 (MQ=255)
                                                                                                                                      aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg    >  1:547690/1‑150 (MQ=255)
                                                                                                                                      aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg    >  3:1510482/1‑150 (MQ=255)
                                                                                                                                      aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg    >  5:705541/1‑150 (MQ=255)
                                                                                                                                       aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCACGCGTCCGAGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTGTTTTTAACACGATCACGGAt  >  3:1348122/1‑151 (MQ=21)
                                                                                                                                       aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt  >  7:565114/1‑151 (MQ=255)
                                                                                                                                       aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt  >  1:1531905/1‑151 (MQ=255)
                                                                                                                                       aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt  >  6:824196/1‑151 (MQ=255)
                                                                                                                                        |                                                                                                                                                     
CACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAT  >  DH1/936158‑936442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: