Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2970AG27.6% 257.0 / 107.3 138intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (82/15);  new base G (35/2);  total (118/20)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-01

Only 100 of 143 total aligned reads displayed.

CACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936158‑936447
                                                                                                                                            |                                                                                                                                                      
cACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACAGTAATCC‑ATGACTTCATCACATTTTAATTCGTTGc                                                                                                                                               >  4:775942/1‑149 (MQ=17)
                                                                ttCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACGTCATGAAATTTTa                                                                                                                                                        >  5:909856/1‑76 (MQ=255)
                                                                                       tACCAGGCTGCTCCACCCCGCGTCAGGGTAGTGG‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCGGGATTTGAACCGACGACCTTAGGGTTATGATCCAGAAGAGCTACCAGGCTGCTCCCCCCCGAGTCAGTGGATTCGCACtat                                                       >  3:1504848/1‑150 (MQ=17)
                                                                                              ctgctCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCTGATTTGAACCGACGCCCTTCGGGTTATGAGCCAGACGAGCTACCAGGCTGCTCCACCCCGCTTCGGTTGATGGGCACTATACTCCGCt                                               >  1:461826/1‑151 (MQ=17)
                                                                                              ctgctCCACCCCGCGTCACCGTACTGC‑GTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACTATACTCCGcg                                               >  7:1056501/1‑150 (MQ=255)
                                                                                               tgctCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACATTAGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTc                                              >  7:1166016/1‑151 (MQ=255)
                                                                                                  tCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTcgcg                                           >  3:658780/1‑151 (MQ=255)
                                                                                                   ccACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGCGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGt                                          >  5:61396/1‑151 (MQ=255)
                                                                                                    cACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                         >  7:127704/1‑151 (MQ=255)
                                                                                                     aCCCCGCGTCACCGTACTGCTTTTACTTAATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGAGTCGCAGTATACACCCCTCTCGttt                                        >  3:1096078/1‑151 (MQ=11)
                                                                                                     aCCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                                        >  5:996259/1‑151 (MQ=255)
                                                                                                     aCCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATAATCCGCTCGCGttt                                        >  6:575157/1‑151 (MQ=255)
                                                                                                       cccGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGATCGCGttttg                                      >  1:480857/1‑150 (MQ=14)
                                                                                                       cccGCGTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAAGCCGACTATAcatccaaagagttttc                                      >  7:500767/1‑136 (MQ=11)
                                                                                                        ccGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                      >  2:434263/1‑150 (MQ=255)
                                                                                                         cgcgTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCAt                                    >  7:673001/1‑151 (MQ=255)
                                                                                                           cgTCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGGGCAGTATACTACAATCGTGTTTTcgttg                                  >  5:719431/1‑147 (MQ=11)
                                                                                                             tatctgtacttcttttACTT‑ATTAAATTTTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACGAGGCTGCTCCACCGCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                                 <  8:873305/137‑1 (MQ=18)
                                                                                                             tCACCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTACGCTAGAGTTttcattca                                 >  8:1201263/1‑150 (MQ=14)
                                                                                                             tCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                                 >  6:1149148/1‑150 (MQ=255)
                                                                                                              cACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGTCGACCTTCGCGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAc                               <  5:1373289/151‑1 (MQ=255)
                                                                                                               aCCGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAATATACACCAATCGCTTATTCATGCAAcc                              >  3:1414503/1‑151 (MQ=11)
                                                                                                               aCCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                              >  7:946638/1‑151 (MQ=255)
                                                                                                                ccGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGGAATATACTACGCTCGAGATTTCATGCAACCt                             >  4:971276/1‑151 (MQ=11)
                                                                                                                ccGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGCGCCCGACGAGCTACCAGCCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                             >  2:1189175/1‑151 (MQ=255)
                                                                                                                ccGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                             >  6:281523/1‑151 (MQ=255)
                                                                                                                 cGTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGTCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGATCGCGTTTTCATGCACCCtt                            >  1:301676/1‑151 (MQ=14)
                                                                                                                 cGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                            >  2:810800/1‑151 (MQ=255)
                                                                                                                  gTACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGCCGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAGTGGGAGACTACTCCGCTCGCTTTTTcgttccgggggg                           >  6:689598/1‑140 (MQ=11)
                                                                                                                   tACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCaa                                <  1:1491998/145‑1 (MQ=255)
                                                                                                                   tACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTAGCGTTTTCATGCaaagatcc                          >  2:1508779/1‑145 (MQ=14)
                                                                                                                   tACTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCACTATACTCCCCTCGAGTTTTCATGCAACCtttt                          >  2:876605/1‑151 (MQ=14)
                                                                                                                   tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                          >  1:109663/1‑151 (MQ=255)
                                                                                                                   tACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttt                          >  7:1499944/1‑151 (MQ=255)
                                                                                                                    aCTGCTTTTACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAAGCGGTCTATTCTCCGATCGAGttttgtttcacccgtttt                         >  3:1040012/1‑137 (MQ=11)
                                                                                                                    aCTGC‑TTTACTTCATCAAATTTAAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCTGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttt                         <  8:1062321/151‑1 (MQ=255)
                                                                                                                     ctgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGAGTATACTCCTCAAGAGTTTTCATGCATCCttgatt                        >  5:460265/6‑147 (MQ=11)
                                                                                                                     ctgcttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATTCGCTCTATACTACGCTCGAGTTTTCCTGCAACCtttttt                        >  1:361968/6‑151 (MQ=14)
                                                                                                                      tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                       >  7:124214/1‑151 (MQ=255)
                                                                                                                      tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                       >  8:764472/1‑151 (MQ=255)
                                                                                                                      tGC‑TTTACTTCATCAAATTTTAATTGGTTGCGCGGCCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGCTGCGCACTATACTCCGCTCGCGGTTTCATGCAACCtgttttt                       >  6:97702/1‑151 (MQ=255)
                                                                                                                       gC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                      >  4:1380917/1‑151 (MQ=255)
                                                                                                                        cttttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCtttttttt                      >  1:822043/3‑150 (MQ=255)
                                                                                                                        c‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttttt                      >  3:1025057/1‑150 (MQ=255)
                                                                                                                          tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCCCTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAc                    >  8:1286783/1‑151 (MQ=255)
                                                                                                                          tttACATCATCAAATTTTAATTGGTAGCGGGGGCCGGATATGAACCGACGACCTTCGGGTTATCAGCCCGACGAGCTACCAGGCTGCTCCACCACGCGTCAGTGGAAGCGCACTATAATACGCTCGCGTATTCATGCAACCTATTTTTTa                     >  6:912534/1‑150 (MQ=18)
                                                                                                                           ttACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTACGATAGCGTTTTCATTCAACCTTATTTTTa                     >  6:1312007/1‑148 (MQ=17)
                                                                                                                            tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc                  >  4:752069/1‑151 (MQ=255)
                                                                                                                            tACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGCACTATACTCCTCTCTATTTTTCATTCAGCGTTTTTTTTTccc                  >  7:1440116/1‑150 (MQ=14)
                                                                                                                            tACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAGGGGGTGTATACTCCGCTGGGGTTTTCGTGCAGCGGTGTTTTTaggcc                 >  7:232116/1‑147 (MQ=1)
                                                                                                                            tACTTCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAATCCGTCTATACACCCCTTGCTTTTTCATTCAACGTTTATTTTaaca                  >  1:74197/1‑147 (MQ=1)
                                                                                                                            cgttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGAGGCGCACTATACGCCGATCGAGTTTTCATACCACCTGTTTTTTAccc                  >  3:592365/16‑151 (MQ=14)
                                                                                                                             gttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAAGCCAACTATACAACCATAGCGTATTCATGCACCCTTGTTTATAaccc                 >  8:446550/15‑151 (MQ=11)
                                                                                                                              ttagggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                        <  8:60803/130‑1 (MQ=255)
                                                                                                                               ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCgc               >  5:382682/1‑150 (MQ=255)
                                                                                                                               ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                >  1:1423733/1‑150 (MQ=255)
                                                                                                                               ttCATCAAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACTGGATTCCAAAAATACTACAATCTATTTTTCATTCAACCTTTTTTTTaacaaga               >  2:589688/1‑144 (MQ=11)
                                                                                                                                tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                >  4:853064/1‑149 (MQ=255)
                                                                                                                                tcatcaAATTTGAA‑TGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGATATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGACCCAGGAAGCGCAGAATACTACACAGGAGATTTCCTGCAGCCTGATTATTACCCCGaac             >  4:1114900/1‑149 (MQ=2)
                                                                                                                                tcatcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAGTATACTCCGCTCGCGATTTCCTTGCACCGTTGTTTTAGCCCCAt              >  5:992320/1‑150 (MQ=14)
                                                                                                                                tcatcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGGACTATACTACGATCGAGTTTTCATGCAACCTTATTTTTAACCCCATc             >  4:158620/1‑151 (MQ=17)
                                                                                                                                tcatcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCGGACTATACTCCGCTCGAGTTTTCATGCACCCTTTGTTTTACCCCGATc             >  5:1045065/1‑151 (MQ=18)
                                                                                                                                attacgatctatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                <  5:1393921/138‑1 (MQ=255)
                                                                                                                                agggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                         >  3:1518726/12‑124 (MQ=255)
                                                                                                                                agggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtt                                         <  4:1532093/113‑1 (MQ=255)
                                                                                                                                 taataaaTTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt              <  8:1187204/146‑1 (MQ=255)
                                                                                                                                  ggtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCa            <  6:558485/142‑1 (MQ=255)
                                                                                                                                  atcaAA‑TTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATGCGAAAAATACTCCGCTAGATTATTCATGCAACCTTTATTTTAGCCACATCAc           >  8:793023/1‑151 (MQ=14)
                                                                                                                                   tcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGCCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGGTTTCATGCAACCTTTTTTTTACCCCGATCAc           >  4:1378858/1‑151 (MQ=255)
                                                                                                                                   tcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc           >  2:279944/1‑151 (MQ=255)
                                                                                                                                   tcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc           >  3:87021/1‑151 (MQ=255)
                                                                                                                                   gtgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCAc           <  2:1001437/143‑1 (MQ=255)
                                                                                                                                    tgttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCGTTTTTTTACCCAGATCACg          >  1:1180587/8‑151 (MQ=255)
                                                                                                                                    caAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACg          >  2:652486/1‑151 (MQ=255)
                                                                                                                                     gttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCTGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCg                <  6:868218/138‑1 (MQ=255)
                                                                                                                                     gttcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTCGCGTTTTCATGGAACCTTTTTTTTACCCCGATCACgg         >  3:1144680/7‑151 (MQ=255)
                                                                                                                                     aaaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  8:1082396/1‑151 (MQ=255)
                                                                                                                                      aaatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAATATACTCCGATCGCTATTTCATGCAACCTTTTTTTTACCCCCATCACgc         >  4:845159/4‑149 (MQ=18)
                                                                                                                                      aaatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGAAGCGCAGTATACACCGCACTAGTTTTCATGCAACCTTATTTTTAACCAGATCACgc         >  2:1094683/4‑149 (MQ=17)
                                                                                                                                      aaatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCAGTGGATGCGAACTATACTCCGATATAGTTTTCATTCAACCtt                            >  2:120976/4‑131 (MQ=14)
                                                                                                                                      aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  3:1510482/1‑150 (MQ=255)
                                                                                                                                      aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  1:547690/1‑150 (MQ=255)
                                                                                                                                      aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  1:1256683/1‑150 (MQ=255)
                                                                                                                                      aaTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg         >  5:705541/1‑150 (MQ=255)
                                                                                                                                       tcaatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGTATGCGCACTATACTCCGCTCGAGTTTTCATGCAACCTTTATTTTACCCAGATCACGGAt       >  4:1094324/5‑151 (MQ=255)
                                                                                                                                       aatttGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCCCTGTATACTCCGCTCGCTTTTTCATGCAACCGTGTTTTTACCCCGATCAGGGAt       >  7:205060/3‑151 (MQ=18)
                                                                                                                                       aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCACGCGTCCGAGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTGTTTTTAACACGATCACGGAt       >  3:1348122/1‑151 (MQ=21)
                                                                                                                                       aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt       >  7:565114/1‑151 (MQ=255)
                                                                                                                                       aTTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAt       >  6:824196/1‑151 (MQ=255)
                                                                                                                                        caatAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAGGCGCACtat                                                       >  8:774552/4‑102 (MQ=255)
                                                                                                                                         tttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAttt     >  7:584776/1‑151 (MQ=255)
                                                                                                                                         attAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAgg                                                                                        <  5:109558/67‑1 (MQ=14)
                                                                                                                                          ttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTg    >  2:849431/1‑151 (MQ=255)
                                                                                                                                          atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAGTATACTCCGCTCGCGTTTTCCTGCACCCTTTTTTTTACGCCGATCCCGGATTTg    >  3:3260/2‑151 (MQ=255)
                                                                                                                                          atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTg    <  3:894413/150‑1 (MQ=255)
                                                                                                                                          atAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACACCGCTCTCTTATTCATTCAACCTTTTTTTTaacaag                >  6:1399403/2‑134 (MQ=18)
                                                                                                                                           tAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGTATACCCAATATACTACCATCTCGTATTCATGCAACCTTATTAATAACCCCATCACACATTTgg   >  4:201349/1‑151 (MQ=14)
                                                                                                                                            aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGc  >  5:897456/1‑151 (MQ=255)
                                                                                                                                            aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGc  >  7:1281892/1‑151 (MQ=255)
                                                                                                                                            aaTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAACCCGATCACGGATTTGGc  >  6:1171632/1‑151 (MQ=255)
                                                                                                                                            |                                                                                                                                                      
CACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGC‑TTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATTTGGC  >  DH1/936158‑936447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑