Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,591,5130TG24.1% 242.2 / 38.7 241D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (89/65);  new base G (17/32);  total (120/121)
Fisher's exact test for biased strand distribution p-value = 5.40e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

ACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAGGATG  >  DH1/2591364‑2591660
                                                                                                                                                     |                                                                                                                                                    
                        cgttgTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGc                                                                                                                             <  2:11197/146‑1 (MQ=255)
                        cTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTAGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGc                                                                                                                             >  2:730071/1‑150 (MQ=255)
                         tGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGACTAGAGCGACAGCGAGAGAGCGTTGC‑CGCAGGCa                                                                                                                            >  4:497405/1‑150 (MQ=255)
                          ttgTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAATCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCaa                                                                                                                           <  8:1212995/148‑1 (MQ=255)
                            gTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAACCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGa                                                                                                                        <  3:1438444/151‑1 (MQ=255)
                             tattCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATTAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGa                                                                                                                        <  8:1030777/147‑1 (MQ=255)
                              aaTCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGGCTAGAGCGGCAGCGAGAGAGCGTTGC‑CGCAggagagcgg                                                                                                                       >  7:64111/1‑143 (MQ=14)
                                tCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTGGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCg                                                                                                                    >  6:885685/1‑151 (MQ=255)
                                 ccTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTTGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCg                                                                                                                    >  1:1150597/1‑150 (MQ=255)
                                   ttaccccTCCACCACCATTTTCAAAATTTCGTGAAATTTTATTTCAATCCCCTATCATTTTTTGTTCTCATGGGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGaa                                                                                                                  <  8:1076013/147‑1 (MQ=2)
                                   ttCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTAACAAACGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAgg                                                                                                                              >  4:115926/1‑138 (MQ=255)
                                    tCCCCCACCACCATCACTTTAAAAAGTCCTTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGTAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGaa                                                                                                                  <  8:101437/149‑1 (MQ=255)
                                     cccccACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAggg                                                                                                               <  6:560763/151‑1 (MQ=255)
                                     cccccACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGGCAGCGAGAGCCCGTTGC‑CGCAGGCAACGACCCGAAggg                                                                                                               >  5:1136505/1‑151 (MQ=255)
                                     ccccaACCACAATCACTTTAAAAATTCCCTGAACTTACAACCTAATCCGAAATAAAATATTCTGCCCATGCGGGTATGTATGAGAAGCTTCTACTAAGGTTCGGCTCGAGCGCCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAggg                                                                                                               <  2:495963/151‑1 (MQ=11)
                                      ccccACCACCATCACTTTCAAAAGTCCCTGAACTCACAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGc                                                                                                              <  3:145793/151‑1 (MQ=255)
                                        ccacaactaTCATTTTTAAAAGTCCTTTAACTTTAAATCGAATCCGCAATAAAATATTTTGCCCATGGGGGGATGGATTATAAGCTTCGACTAAGGTTCGTCTCGAGGGTCAGCTAGAGTGGGTTGC‑CGCAGGCAACGACCCGAAGGGCGa                                                                                                            <  8:318042/151‑1 (MQ=2)
                                         cctacccaTCACTTTCAAAAGTCCCTGAACTCCCCACCGAATCCGCAATAAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTGCGGCTCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGaa                                                                                                           <  1:1276425/146‑1 (MQ=11)
                                         caccaccaTCACTTTCAAAAGTCCCTGAACTTTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGaa                                                                                                           <  2:838047/151‑1 (MQ=255)
                                         caccaccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACAAAGGTTCGGCTAGAGCGACAGCGAGAGAGCGTTGC‑CGCAGGCAACGAcagaacggaaga                                                                                                            >  2:1331424/1‑139 (MQ=14)
                                          accaccaTCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGa                                                                                                            >  4:1288269/1‑149 (MQ=255)
                                                 tCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCac                                                                                                   >  7:920290/1‑150 (MQ=255)
                                                  cccttacaaaccccccTCAACTCGCAACCGAATCCCCAATCAAATAGTCTCCCCATGCGGCGAAGGATGAGAAGCTTCGACTAAGGTGCGGCGCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCa                                                                                                    <  3:539796/136‑1 (MQ=14)
                                                  cACTTTCAAACGTCCCTGAACTCTCAACCGAATCCGCAATCAAATATTCTGCCCCTGCGGGGAAGGATGAGAAGCTTCGACTAAGGTGCGGCTCGAGCGTCCGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgc                                                                                                  <  5:1560502/151‑1 (MQ=255)
                                                   aCTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTGGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgcg                                                                                                 >  3:1287960/1‑151 (MQ=255)
                                                   aCTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgcg                                                                                                 >  5:823773/1‑151 (MQ=255)
                                                   aCTTTCAAAAGGCCCTGAACTCTCAACCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgcg                                                                                                 <  3:591678/151‑1 (MQ=255)
                                                    cTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAgcgc                                                                                                >  2:1447359/1‑151 (MQ=255)
                                                    cTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGCCTCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAgcgcgagggcg                                                                                                >  6:628796/1‑145 (MQ=255)
                                                     tttCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACGAAGGTTCGGCTAGAGCGACAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCt                                                                                               >  2:291370/1‑151 (MQ=255)
                                                       tCAAAAGGCCCTCAACTCCCAACCCAAGCCCCAAAAAAATATTCTGCCCATGCGGGGACGGATGAGAAGCTTCGACTAAGGTTCGCCGCGCGCGCCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTg                                                                                              <  5:534367/150‑1 (MQ=11)
                                                        cAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTg                                                                                              >  2:212990/1‑149 (MQ=255)
                                                        cAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTg                                                                                              >  4:345958/1‑149 (MQ=255)
                                                         aaaaGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGt                                                                                           >  7:776766/1‑151 (MQ=255)
                                                           aaGTCCCTGAACTCTCAACCGAATCCGCAATCAAATATTCTGCCCATGGGGGGAAGGATGAGAAGCTTCGACTATGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTaa                                                                                         <  8:520750/151‑1 (MQ=255)
                                                            aggccatcaaCTCTCCCCCGAATCCCCAAGCAAAAATTCTGCCCCTGCGCGGAAGGATGAGAAGCTTCGACCAAGGTTCGCCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAAt                                                                                        <  7:908585/143‑1 (MQ=11)
                                                            aGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAAt                                                                                        >  6:1560880/1‑151 (MQ=255)
                                                              tCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATcc                                                                                      >  5:660546/1‑151 (MQ=255)
                                                               cccTTAACTATCAATCTAATCCGCAATTAAATTTTCTGCCCATGCGGGTAAGTATGAGAAGCTTCGACTATGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCt                                                                                     <  4:257218/151‑1 (MQ=11)
                                                               cccTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCt                                                                                     >  1:280396/1‑151 (MQ=255)
                                                               cccTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACCAAGGTTCGACTCGAGCGACAGCCTGGGAACCGTCC‑AGCAGTCAACGCCACGAACGCATAAGATCCCAGCGCTGAGTAATCCt                                                                                     >  7:581051/1‑151 (MQ=2)
                                                                ccTGAACTCCCAACCGAATCCGCAATAAAATATTCTGCCCAGGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGCGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCtt                                                                                    <  7:156411/151‑1 (MQ=11)
                                                                 cTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTc                                                                                   >  3:154584/1‑151 (MQ=255)
                                                                 cTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTc                                                                                   >  7:1405000/1‑151 (MQ=255)
                                                                 cTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTc                                                                                   >  4:1007384/1‑151 (MQ=255)
                                                                  tGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTcc                                                                                  >  8:904062/1‑151 (MQ=255)
                                                                   cacctcaCAACCCAAGCCGAAATAAAATTTTCGCCCCCTGGGGGGACGGAGGAGACGCTGCGACAACGGTGCGCCGCGCGCGCCCGCGAGAGCGGGTGCC‑CGCAGCCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTc                                                                                   <  7:869043/146‑1 (MQ=2)
                                                                    aaCTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTcccc                                                                                >  3:1131599/1‑151 (MQ=255)
                                                                      ctctCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTccccc                                                                               >  8:722103/1‑150 (MQ=255)
                                                                      ctccccaccGAATCCGCAATAAAATATTCTGCCCTTGCGGGGAAGGATGAGAAGCTTCGACTACGGTGCGGCGCGCGCGCCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTccc                                                                                 <  5:1222613/140‑1 (MQ=11)
                                                                          cAAGCGAAACCCCAATCAAATATTCTGCCCATGAGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCCAGAGACAGCCAGAGACCGTTGC‑CGCAGGCAACGACCCGAAGTGCGAAGCGCGCAGCGCTGAGTAATCCTTCACccaccac                                                                          >  8:1474700/1‑151 (MQ=14)
                                                                            aGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTGGAGCGACAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTATTCCTTCCCccaccacc                                                                         >  5:1467759/1‑150 (MQ=255)
                                                                              gaagtCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTGGGCCTGGAGCGTCAGGGAGTGAGGGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATc                                                                      <  5:1043743/147‑1 (MQ=11)
                                                                              cGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATc                                                                      >  2:584980/1‑151 (MQ=38)
                                                                                   ccGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCtt                                                                  >  4:1541502/1‑150 (MQ=38)
                                                                                   acGCAATCAAATATTATGCCCATGCGCGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGA‑CGCAGGCAAAGACCCGAAGGGCGAAGCGCCCAGACCTGAGTAATCCATACCCCACCACCATCAcat                                                                  >  6:1046297/2‑148 (MQ=14)
                                                                                    cGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCttcc                                                                >  5:126348/1‑149 (MQ=37)
                                                                                       aaTCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCAt                                                                       >  7:1151644/1‑141 (MQ=38)
                                                                                        gacaaatttagtGCGCATGGGGGTTAGGATGTGAGCTTTCCCCTACGGTTCGGCTCGCGCGCCAGCGATAGAGGGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCaaaa                                                            <  3:1024892/140‑1 (MQ=11)
                                                                                          taaaTATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGc                                                                                                                                                  <  2:39386/63‑1 (MQ=255)
                                                                                          cAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATcgagatcgcaagc                                                          >  6:681505/1‑139 (MQ=255)
                                                                                           aaaTATTCTGCCCATGCGGGTAAGTATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGcc                                                         <  8:542626/151‑1 (MQ=255)
                                                                                             atatTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGACAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGAGCAGCGCTGAGTAATCCTTCCACCACCACCATCACTTACaaaaaaac                                                        >  6:196952/1‑146 (MQ=11)
                                                                                                ttCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATc                                                                      >  3:21822/1‑133 (MQ=37)
                                                                                                 tCTGCCCATCCGGGGAAGGAGGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGa                                                    <  3:271495/150‑1 (MQ=255)
                                                                                                   tGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCCCTTCCaaaaaaacaccaatc                                                 >  3:1041181/1‑140 (MQ=11)
                                                                                                   ggctgccgctctgAAGGATGAGAAGCTTCGACCAAGGTTCGCCTCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAAtt                                                 <  5:261665/139‑1 (MQ=11)
                                                                                                    gCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGTCATCTATTTAGCGTTGC‑GGCATGCAACGACCCGAAGTGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGaa                                                   <  8:822073/148‑1 (MQ=14)
                                                                                                    gCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGGCAGCTAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGaa                                                   <  3:230173/148‑1 (MQ=255)
                                                                                                    gCCAATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGCCTCGAGCGGCAGCGATAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAAtt                                                 <  5:340225/150‑1 (MQ=255)
                                                                                                     gccttGCTGGTAAGGATGAGAAGCTTCGACTAAGGTTCGCCGCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATtct                                               <  7:356715/147‑1 (MQ=14)
                                                                                                     agcttGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATtct                                               <  1:1081846/147‑1 (MQ=255)
                                                                                                      ccATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATtctc                                              <  5:444531/151‑1 (MQ=255)
                                                                                                        aTGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCttc                                                                 >  1:862654/1‑129 (MQ=37)
                                                                                                          gCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAAc                                          <  1:209976/151‑1 (MQ=255)
                                                                                                            gggtAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGa                                        <  5:1356607/151‑1 (MQ=255)
                                                                                                            ggggAAGTATGAGAAGCTTCGACTAAGGTTCGTCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGa                                        <  4:619192/151‑1 (MQ=255)
                                                                                                              ggatcttggtttatcTTCGACAAAGGTTCGACTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAAt                                      <  6:1464135/137‑1 (MQ=14)
                                                                                                              ggAAGGATGAGACGCTGCGACAAAGGTTCGGCTCGAGCGCCAGCGATAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAAt                                      <  7:837952/151‑1 (MQ=255)
                                                                                                                  ggATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCTTGCAAAAGCAACGGAAATCTCATACGACTcccc                                  >  1:463730/1‑149 (MQ=14)
                                                                                                                   gctctgccgatgggACCAAGGTGCGCCTCGAGCGCCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCa                                 <  3:995123/138‑1 (MQ=11)
                                                                                                                      gagaAGCTTCGACTAAGGTTCGTCTCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATc                              <  4:186612/151‑1 (MQ=255)
                                                                                                                         aaGCTTCTACTAAGGTTCGTCTCGAGCGTCAGCTAGAGAGCGTTGC‑CGCAGTAAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCaa                            <  2:1030159/150‑1 (MQ=255)
                                                                                                                          cgCTTCGACTAAGGTGCGGCTCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAat                          <  3:394441/150‑1 (MQ=255)
                                                                                                                           tcctatatcaaGGTTCGACTCGAGCGCCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATc                              <  4:149902/137‑1 (MQ=255)
                                                                                                                           gCTTCGACCAAGGGTCGCCTCGAGCGTCAGCCAGAGAGCGTTCC‑CCCAGGCAACGACCCGAAGGTCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGGCCCGGAATTCTCAAACGAATCCGCAATCAAata                         <  3:1335456/151‑1 (MQ=11)
                                                                                                                             ttCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCGCtt                                                                  >  1:303997/1‑108 (MQ=255)
                                                                                                                             ttCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCCCTTTCAAAAGGCACGGAATTCTCATACGCATCCGGAATctattaat                       >  7:394643/1‑144 (MQ=14)
                                                                                                                              tCGACTAAGGTTCGCCTCGAGCGCCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAatat                        <  1:1434505/149‑1 (MQ=255)
                                                                                                                               cGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATACATCCCAAACCCCCCGCAGTTTCAAAAGCCCCGGAATTATCAAAAGAAGCCGCAATGAAAGAttat                     >  6:275367/1‑149 (MQ=14)
                                                                                                                                gACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTACAAAAGCAAACGAATTCTCAAACTCCTCCCCAATGAAACATTCTg                    >  1:1033508/1‑151 (MQ=14)
                                                                                                                                   tAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGccc                 >  6:1130802/1‑151 (MQ=255)
                                                                                                                                       gTTCGCCTCGAGCGCCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGc             <  1:708177/151‑1 (MQ=255)
                                                                                                                                       gTTCGACTCGAGCGCCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGc             <  8:619770/151‑1 (MQ=25)
                                                                                                                                        gggggCTCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCg            <  3:518052/148‑1 (MQ=255)
                                                                                                                                         tCGGCTCGAGCGGCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCg            <  3:849541/150‑1 (MQ=255)
                                                                                                                                         gcgccgggggggTCAGCGAGCGCGCCTTGCGCGC‑TGCAACGACCCGAAGGGCGATGCGCGCAGCGCGGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCg            <  5:93215/139‑1 (MQ=14)
                                                                                                                                            attCGAGCGAAAGCGAGAGAACGTTGC‑CACAGGCAACGGCCCGAAGGGTGAGGAGCGAAGCGACGAATAATCCTTCCCCCACCACCATCACATTCAAAAGAACAAGAAATCACAAACCAATCCGCAATGAAAGATTCTGCCCatgggatcg        >  6:1199678/3‑143 (MQ=9)
                                                                                                                                               cGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCGGGGa        <  6:981553/148‑1 (MQ=255)
                                                                                                                                                 aGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTACCCCACCACCATCACTTTCAAAAGCCCCCGAATTCTCAAACGAATCCGCAATGAAATATTCTGAGCAAGCGGGGAAGGAt   >  5:1414755/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCCTGAACTCTCAAGCGAATCCGCAATCAAATATTCTGCCCATGCGGGGAAGGATGAGAAGCTTCGACTAAGGTTCGGCTCGAGCGTCAGCGAGAGAGCGTTGC‑CGCAGGCAACGACCCGAAGGGCGAAGCGCGCAGCGCTGAGTAATCCTTCCCCCACCACCATCACTTTCAAAAGCCCCGGAATTCTCAAACGAATCCGCAATCAAATATTCTGCCCAAGCGGGGAAGGATG  >  DH1/2591364‑2591660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑