Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,184,5850CA26.9% 245.1 / 34.8 135intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
Reads supporting (aligned to +/- strand):  ref base C (60/38);  new base A (33/3);  total (93/42)
Fisher's exact test for biased strand distribution p-value = 5.69e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

AATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184437‑3184734
                                                                                                                                                    |                                                                                                                                                     
aaTCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACACCAGCCAAATTAATct                                                                                                                                                      >  5:752975/1‑149 (MQ=255)
    ccGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACg                                                                                                                                                 >  7:345249/1‑151 (MQ=255)
       tcgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTATTAAAGagggct                                                                                                                                              >  5:62304/1‑146 (MQ=255)
       tcgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAGatcgct                                                                                                                                              >  3:1186818/1‑146 (MQ=255)
            gCCACCAAATTCTGAATGTATCGAATATGTTCTGCAAATTCAAAACCAATTTGTTGTGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCtt                                                                                                                                         <  3:484874/151‑1 (MQ=255)
              caccaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATAAAGACGCTTAcggggt                                                                                                                                       >  5:187734/1‑146 (MQ=255)
                ccaTATTCTGAATGTATGGAATATGTTTTGCAAATTCAAAACCTTTTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGt                                                                                                                                       <  6:949417/149‑1 (MQ=25)
                      tCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATAAAGATCCTTTCCGGGTAAGTGAAc                                                                                                                               >  7:388621/1‑151 (MQ=18)
                        tGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGAACCTTACCTGGTAAGTGCAccc                                                                                                                             >  7:285629/1‑151 (MQ=255)
                         gAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCt                                                                                                                                               <  1:88652/132‑1 (MQ=255)
                         gAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCt                                                                                                                                               >  2:88481/1‑132 (MQ=255)
                         gAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGAGCGTTACGTGGTAAGTggtccct                                                                                                                            >  7:1201271/1‑145 (MQ=18)
                         gAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCATGGTACCCCACCCAATTTAAGCAAGAGCCTGACCTGGTAAGTGCAcacc                                                                                                                            >  3:1534260/1‑148 (MQ=21)
                           aTGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAGAGCCGTACCTGGATAGTGCTccgatt                                                                                                                          >  3:715850/1‑147 (MQ=18)
                           aTGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAGAAGCATACCTGGTCAGTGCAGCCatt                                                                                                                          >  3:644081/1‑149 (MQ=18)
                                  gAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTa                                                                                                                   <  3:801648/151‑1 (MQ=255)
                                      attttCGGCAAATTCAAACGCAATGTCTTGGGGAATCGCGCAGCGGTAAGCCCCCGGATTCTGATGCCCGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACCCTTACCTTGTAAGTGCACCCAGTTGGGGTATCg                                                                                                                <  5:1177145/147‑1 (MQ=25)
                                      acgtttttgaacatCACAACGATTTTATTGGGGTATCGCCAAGCGGTAAGGCACCGGATTGTGTTTCCTTCATTCCGTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTAt                                                                                                                  <  7:421188/135‑1 (MQ=21)
                                         ttcttcAAATTCAAAACCAATTTGTTGGGTTATAGCCAAGCTGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCa                                                                                                             <  4:1140213/145‑1 (MQ=255)
                                               aaaTTCAATATCATTTTGTTCGGGTTTCTCATATCGTTAATGCTCAGGATTCTGATTGAGGCATTACGCGGTTCGTATCCTCGTACCCCAGCATATTTATTCAATACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTa                                                                                                      <  4:111456/151‑1 (MQ=255)
                                                aaTTCAAAACCTATGTGTGGGGGTATCGCCACCCGTGAAGGCACAGGATTCTGACTCCGGCATTCAGAGCGTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTCACTTGGAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTaa                                                                                                     <  7:1528241/151‑1 (MQ=25)
                                                     aaaaCCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGCAGCATACCGTGTAAGTGCACCCAGTTGGGGTATCGCGACGCGGttaccg                                                                                                  >  7:1389644/1‑144 (MQ=21)
                                                        gttgtacggatgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTgg                                                                                                                       <  1:154365/115‑1 (MQ=255)
                                                        gcccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCAGGTTCGAATCCACGTACCCCAGCCAATTTATTCAAGACGCTTACCATGTAAg                                                                                                                                    >  2:1480642/10‑112 (MQ=11)
                                                         ccctgttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTa                                                                                                                                             <  2:1145884/94‑1 (MQ=255)
                                                         cccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATTCCGGCATTCCGAGGTTCGAATCCACGTACCCCAGCCAATTTATTCAAGAGGCTTa                                                                                                                                             >  1:1135197/9‑102 (MQ=14)
                                                           cggttattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACg                                                                                                                                                 >  1:1031768/7‑96 (MQ=255)
                                                            ggttattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTATGATACCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTCaa                                                                                                                                                     >  8:1083949/6‑91 (MQ=14)
                                                            ggttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCaa                                                                                                                                                     <  7:1078177/86‑1 (MQ=255)
                                                                attgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCAAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAAGCTTACCTTGTAAGTGCTCCCAGttcg                                                                                                                       >  1:306841/2‑115 (MQ=18)
                                                                 ttgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCt                                                                                                                                               >  5:1519373/1‑92 (MQ=14)
                                                                 ttgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGAAGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGACAAGCGGTAAGGCACCGGATTCTAAtt                                                                                    >  5:176764/1‑151 (MQ=255)
                                                                       tATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGc                                                                               >  7:875272/1‑150 (MQ=255)
                                                                        aTCGCCAAGCGGTAAGGCACCGGATTCTCATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTATACAAGAGCCCTTCCTGGAGAGTGCTCCCAGTCGGGGTATCGGCAAGCTGGAAGGCCCCGGAGACTACTCGCGGCAt                                                                             >  3:1523205/1‑151 (MQ=9)
                                                                         ggcccgacccgcacggCACCTGATTCTGATACCGGCATTCCTAGTTTATAATACTCTTACGGCATCCAATTTATTCTAGACTCTTACCTTGTAAGTTCACCTAGTTGTGGTATCTCCAAGCTGTAAGGCACCGGATTCTAATTCCGGCAtt                                                                            <  4:1345796/137‑1 (MQ=255)
                                                                          cGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAAGCTTACCTTGTAAGTGAACCGAGTTTGGGTATCGACAAGCTGTAAGGCACCGGATTCTAATTCCGTCATTc                                                                           >  4:1554866/1‑151 (MQ=25)
                                                                             cAAGCGGTAAGGCACCGGTTTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCt                                                                                                                                          <  2:938517/85‑1 (MQ=255)
                                                                              aatgggTAAGTCTACGGTTTTTGATTCTGGTATTAATAGGTTCGTATCCTCGTAACTCAGAATATTTATTAAATACTCTTAAATTTTAATTGCAATTAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAg                                                                       <  8:56037/147‑1 (MQ=9)
                                                                               acctggaatcatcatgATTCTGACTCAGGCACTACTAGCTTCGTATCCTCGTAATCCAGAAAATATATTCTATCCGCTTACATTGTAACTGCACCGAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAgg                                                                      <  5:608651/136‑1 (MQ=12)
                                                                                 cGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGATTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAg                                                                                                           >  6:1129976/1‑112 (MQ=21)
                                                                                 cGGTAAGGCACCGGTTTTTGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGATTACATTGTaatt                                                                                                                                   >  8:1417734/1‑86 (MQ=17)
                                                                                 cGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGt                                                                                                                                   <  7:1411283/88‑1 (MQ=255)
                                                                                 cGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAg                                                                                                           <  5:1127242/112‑1 (MQ=255)
                                                                                 cGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAAGCTTACCTAGTCAGTGAAGCCTGTTGGGGTATCGACAAGCTCTAAGGCACGCTAGACTAATACCGTCCTTCCGAgtat                                                                    >  8:577814/1‑148 (MQ=18)
                                                                                    tAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGCATACCTAGTAAGTGAACCAAGTTTGGGTATAGCCAATCGGTAATGCACAGGATTCTAATACAGGCATTCCGAgtatcg                                                                  >  4:1552424/1‑145 (MQ=21)
                                                                                      aGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGCAGCGTACCGTGAAAGTGAACCGAGTGGGCGTTAAGGCAAGCTGTAAGGCGACGGTGTCTAATGGCGTCATTccctgtctccacc                                                               >  5:907037/1‑140 (MQ=2)
                                                                                        gCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCTGGTACCCCAGCCAATTTATTCAAg                                                                                                                                                    >  4:671905/1‑64 (MQ=1)
                                                                                         cacagcaTTCTGTATCAGGCTCTCCGCGGTTCGTATCCACGTACTGCTGAATTTTTCTTCTAGACGCTTTACTTTGAATTTCACCGAGTTGGGGTATCTCCAAGCGGTAAGGCACCGGATTCTCATTCCGGCATTCCGAGGTTCGAATCCt                                                            <  2:1337768/145‑1 (MQ=2)
                                                                                          aCCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAGGGTTACGTGGTAATTGAACCAATTTGGGGTAACGGCAAGCTGTAATGCACCGTATTCTAATGAGAGCATTCAGAGTTTCGagaccgg                                                           >  3:462196/1‑145 (MQ=16)
                                                                                             ggATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGAACCCAGTTGGGGTATCGCCAAGCGGTAAGGCGCGGGAGTCTAATTCCGTCATTCCGAGGTTCGAATCCTcctc                                                        >  7:1180887/1‑148 (MQ=38)
                                                                                              gatggggctaccGGCATTCCGAGGTTCGTATCCTCTTATTTAATCATATTTCTTCAAGAGTCTTATATTGTTAGTGCAACTAGTTTTGGTTTCTCGAAGCGGTTAGGATCTGGTTTTTTATTCCGGCATTCCGAGGTTCGAATCCTCGTAc                                                       <  2:1552741/141‑1 (MQ=255)
                                                                                              gATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTAc                                                       >  7:340421/1‑151 (MQ=255)
                                                                                                    gATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAGAAGCATACGTAGAACTTTAACCAAGTTGGCGTATCGACAAGCGCTAATGCAATGGATACTAATTAAGGCATTCAGTGGATCAAAACAGCGTCCCCCatc                                                 >  8:1335637/1‑149 (MQ=9)
                                                                                                    gATTCAGGCATTCCGAGGTTCGAATCCTCGTACCCCAGACTATATATTCAAGCCGCTTACCTTGTAACAGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGc                                                 <  8:1395415/151‑1 (MQ=25)
                                                                                                       accGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTAcc                                                                                                                                           <  1:671713/57‑1 (MQ=255)
                                                                                                         cGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGCAGCGTACCTGGTAAGTGCACCGAGTTGGGGTATCGCCAAGCTGTAAGGCGCCGTAGTCTAATTAAGTCATTCCCAGGTTCGACTCCTCGTACCGCCGCCACAt                                            >  3:1356495/1‑151 (MQ=25)
                                                                                                         cGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGCTTACGTGGTAAGTGGACCGAGTGGGGGTTGCGGCAAGCGGTAAGGCCCCGGATACTAATTCCGTCATTCCCAGGGTGGAAGCATCGTCCCCCAGCCACAt                                            >  1:1246701/1‑151 (MQ=25)
                                                                                                             aTTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGCGTACCTTGTGAGTGCACCGATTGGGGGTTGAGCCAATCGGGAAGGCTCGGTATTCTGATGGGGGCATTCCGAGTTGCGAGTGCGCGTCCCCCGGCGACtttta                                         >  7:1405930/1‑148 (MQ=255)
                                                                                                             aTTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATAAAGCGGCGTACGTGGTAATTTGACCGTGTGTGCGTAGATACAATCTGTAAGGCGCCGGATACAAGTTCCGGCATTCAGAGGCTCGAAACATCGTCCCCCCGCTACATTaa                                         >  1:860972/1‑150 (MQ=9)
                                                                                                              ttCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGCGTTCCTTGTAAGTGCACCCAGTTGGGGTATCTCCAAGCGCGAATGCGCCGGATACTAATTGCGGCATTCCGAGTGTCGAGCCATGCTCCCCCCGCCTCATTaaa                                        >  3:158830/1‑150 (MQ=255)
                                                                                                                ccGAGGTTCGAATCCTCGTACCCCAGCCAATTTAAAAAAGCACCGTACGTGGAAAGTGAACCGATTGGGGGTAGCTACAAGCTCTAATGCACAGTATACTACTACCGTCATTCCGAGTATCGAGACCACCTCCCCCGTCGACATTaaaca                                      >  6:1330513/1‑148 (MQ=255)
                                                                                                                   aGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTCCCCCAGCCACATTAAAAAAGCt                                  >  7:579728/1‑151 (MQ=255)
                                                                                                                   aGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGGTTACCTGGTAAGTGAACCGAGTGGGCGTAGCGCCAAGCGGTAAGGCACCGGAGTCTAATACCGGCATTCCCAGTAGCCAGACCTCCTCCCCCCGCCAAATAAAAAAAGCt                                  >  3:915082/1‑151 (MQ=255)
                                                                                                                    ggTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAGCCGTACGTGGAAATTGAACCAAGTAGGCGTAGATACAATCTCTAATGCACAGTATTCTAATAAAGTCATTCCTAGTGTCGAATCCTCGTCCCCCAGCAACATTAAAAAAGCTc                                 >  2:362613/1‑151 (MQ=255)
                                                                                                                     gTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTACTGCCGGCATTCCGAGGTTCGACTCCTCGTCCCCCAGCCACATTAAAAAAGCTcc                                >  5:353892/1‑150 (MQ=255)
                                                                                                                      ttCGAATCCTCGTACCCCAGCCAATTTATTCAAGAGGCTTACGTTGTAAGTGGACCCAGTTGGGGTATCGCCAAGCGCTAAGGCACAGGATTCTAATAAAGGCATTCCCAGGTTCGAATCCTCGTCCCCCCGCCACATTAAAAAAGCTCGc                               >  2:279363/1‑151 (MQ=255)
                                                                                                                       gcGAAGCCACCTACCCCAGCCATTTTATTTTATATGCTTCCCTTTCAACTGCAATCTGTTGTGGTATGGCCAAGCTGTAAGTCAACGGATTTTTTTTGAGGTATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCt                              <  4:277642/150‑1 (MQ=12)
                                                                                                                        cGAATCCTCGTACCCCAGCCAATTTATTAAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACAGTATTCTAATTACGGCATTCCGAGGTTCGAATCCTCCTACCCCAGCCTCATTAAAAAAGCTCGCtt                             >  2:78624/1‑151 (MQ=37)
                                                                                                                        cGAATCCTCCTAACCCAGCCACTTTCTTCAAGCCGCTTACCTTGGAAGAGCACGCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCt                              <  3:1259893/150‑1 (MQ=21)
                                                                                                                         gAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTc                            >  3:357548/1‑151 (MQ=255)
                                                                                                                         gAATCCTCGTACCCCAGCCAATTTATTAAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTACGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTc                            >  7:1368302/1‑151 (MQ=255)
                                                                                                                          aagccacgaaCCCCAGCCACTATATAAAAGCCTCTTAAATTTTAACTCCACCGAGTTGGGGTATCGCCAATCGGCAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCg                           <  5:407571/142‑1 (MQ=17)
                                                                                                                            tCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGc                         >  7:704049/1‑151 (MQ=255)
                                                                                                                              cacGTACCCCAGCCAATATATTCAAGCCTCTTAAATTTTAACACCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGa                       <  1:1089913/149‑1 (MQ=21)
                                                                                                                              cTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  5:145645/1‑150 (MQ=255)
                                                                                                                               tCGTAACCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGAGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCATAGTACCCCAGCCACATTAAAAAAGCTCGATTCGGCg                        >  4:855865/1‑149 (MQ=255)
                                                                                                                                cGTACGCCAGCCAATATCTTCAAGCCGCGTCAATTGTAACTGCACGCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGc                     <  3:310386/151‑1 (MQ=21)
                                                                                                                                   aCCCCAGCCACTATCTTCAATTTTCTGACCTTGAAACAGCACCACGTTGGTGTATCGCGATTCGGTATGGCAGCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCt                    <  5:347778/149‑1 (MQ=18)
                                                                                                                                     cccAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttttt                >  7:625532/1‑151 (MQ=255)
                                                                                                                                      ccAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTg               >  5:1215966/1‑151 (MQ=255)
                                                                                                                                      ccAGCCAATTTATTCAAGACGCGTACCATGGAACAGCACCCAGTTGGGGTAGCGCCAAGCGGTAAGGCACCGGATGCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTg               <  3:751981/151‑1 (MQ=25)
                                                                                                                                       cAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGc              >  5:1086732/1‑151 (MQ=255)
                                                                                                                                        aGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCt             >  8:115620/1‑151 (MQ=255)
                                                                                                                                           cAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          >  2:406528/1‑151 (MQ=255)
                                                                                                                                           cAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          >  4:1096334/1‑151 (MQ=255)
                                                                                                                                           cAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          >  6:544218/1‑151 (MQ=255)
                                                                                                                                            aaTTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTc         >  3:1050004/1‑151 (MQ=255)
                                                                                                                                             aTTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCt        >  6:407278/1‑151 (MQ=255)
                                                                                                                                               ttATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGc      >  1:649020/1‑151 (MQ=255)
                                                                                                                                               atcgacAAGCCGCGTCCCTTGCAACACCACCCAGTGGGGGTAGCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGc      <  3:570956/146‑1 (MQ=21)
                                                                                                                                               atcgacAAGCCGCGTACCTTTGAACTCCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTg       <  1:491787/145‑1 (MQ=21)
                                                                                                                                                 cgacAAGCCGCTTAACTTGAAACAGCACCCAGTTGGGGTATCCCCAAGCGGTAAGGCACCGGATGCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTg       <  1:1449418/145‑1 (MQ=21)
                                                                                                                                                  ttCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGtt   >  7:1506266/1‑151 (MQ=255)
                                                                                                                                                  ttCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGtt   >  7:1316831/1‑151 (MQ=255)
                                                                                                                                                  ttCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGtt   >  7:492528/1‑151 (MQ=255)
                                                                                                                                                  ttCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTAACCCAGCCACATTAAAAAAGCTCGCTTCGGCGATCTTTTTGCTTTTCTGAGtt   >  4:1339547/1‑151 (MQ=255)
                                                                                                                                                   tCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAAGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  8:1274314/1‑151 (MQ=255)
                                                                                                                                                    cAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  2:1026975/1‑150 (MQ=255)
                                                                                                                                                    cAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  2:187923/1‑150 (MQ=255)
                                                                                                                                                    cAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGCCGAGCTTTTTGCTTTTCTGCGttc  >  3:988219/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AATCCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGCTTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184437‑3184734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑