Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,447,4030AC22.2% 612.5 / 53.1 293G346G (GGT→GGG) malZalpha‑glycosidase
Reads supporting (aligned to +/- strand):  ref base A (62/166);  new base C (64/1);  total (126/167)
Fisher's exact test for biased strand distribution p-value = 8.07e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

GCCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGCA  >  DH1/3447253‑3447553
                                                                                                                                                      |                                                                                                                                                       
      aTAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTTGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGcc                                                                                                                                                   <  5:325892/151‑1 (MQ=255)
      aTAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGcc                                                                                                                                                   >  4:68134/1‑151 (MQ=255)
        aGTTCATGGCGGCATCTTCCACATCGCCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTc                                                                                                                                                 <  6:937387/151‑1 (MQ=255)
          ttCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGccc                                                                                                                                               >  2:25256/1‑151 (MQ=255)
           tCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTcccc                                                                                                                                              >  1:696899/1‑151 (MQ=255)
            cATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTTCATCGCCAAAATGTTCTCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTccccc                                                                                                                                             <  8:759578/151‑1 (MQ=255)
              tGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGccccccc                                                                                                                                           >  7:326250/1‑149 (MQ=255)
                 gggcATCTTGCACATCGGCCTGTTACCATTGCGGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCTCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCat                                                                                                                                        <  4:85604/150‑1 (MQ=255)
                  ggcAGCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCata                                                                                                                                       <  6:235127/151‑1 (MQ=255)
                   gcATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCatat                                                                                                                                      <  7:5079/151‑1 (MQ=255)
                      tCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCCGCATAT‑Gcc                                                                                                                                  >  6:1408410/1‑150 (MQ=255)
                        ttCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCCGCATAT‑Gcccc                                                                                                                                >  3:826937/1‑151 (MQ=255)
                         tCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑Gcacc                                                                                                                                <  5:1547059/150‑1 (MQ=255)
                         tCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑Gcac                                                                                                                                 <  5:468499/149‑1 (MQ=255)
                           cacaTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCTGCCCCCCCATAA‑Gcaccac                                                                                                                              >  8:1499841/1‑150 (MQ=255)
                             caTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCTCCCCCCGCAAAT‑GCCCCACATc                                                                                                                           >  5:662599/1‑151 (MQ=255)
                              aTCGGCCTGTAACCCTTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATcc                                                                                                                          <  4:23994/151‑1 (MQ=255)
                              aTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCCCCCCCCCCCTCT‑GCCCCACCTcc                                                                                                                          >  3:995079/1‑151 (MQ=255)
                              aTCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATcc                                                                                                                          <  8:36127/151‑1 (MQ=255)
                                   ccTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATccagccg                                                                                                                     <  8:457499/151‑1 (MQ=255)
                                   ccTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATccagccg                                                                                                                     <  7:886315/151‑1 (MQ=255)
                                     tGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCACCGCCTACCCCCGCAAAT‑TCAACACAAccagccgcc                                                                                                                   >  7:1439443/1‑151 (MQ=255)
                                      gTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGAGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATccagccgcca                                                                                                                  <  1:1403344/151‑1 (MQ=255)
                                        aaCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCCGCATAT‑GCCCCACATccagccgcccgc                                                                                                                >  5:747643/1‑151 (MQ=255)
                                         aCCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATccagccgccagcc                                                                                                               <  7:1244154/151‑1 (MQ=255)
                                           cATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCAACACATCCAGCCGCCAGCCGt                                                                                                             >  1:432042/1‑151 (MQ=255)
                                            aTTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGCCCCCCCCATAT‑TCCCCACAAACACCCGCCACCCGTc                                                                                                            >  8:1240515/1‑151 (MQ=255)
                                            aTTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTc                                                                                                            <  3:84642/151‑1 (MQ=255)
                                             ttGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCACCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTcc                                                                                                           >  6:642283/1‑151 (MQ=255)
                                              tGCGGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTcc                                                                                                           <  8:1446185/150‑1 (MQ=255)
                                               gCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCat                                                                                                         <  4:353546/151‑1 (MQ=255)
                                                    gCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTcc                                                                                                    <  1:1326344/151‑1 (MQ=255)
                                                      aTCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCAc                                                                                                  <  8:74143/151‑1 (MQ=255)
                                                         gCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGgc                                                                                               <  5:1234675/151‑1 (MQ=255)
                                                          ccAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGgcg                                                                                              <  2:1011612/151‑1 (MQ=255)
                                                          ccAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGgcg                                                                                              <  3:434214/151‑1 (MQ=255)
                                                            aaaaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCTCCCCCCCCATAT‑GCACCCCCTCCCGCCGCCCGCCGGCCATATTCCACCgcgcg                                                                                            >  5:996260/1‑150 (MQ=255)
                                                            aaaaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCt                                                                                            <  4:592787/151‑1 (MQ=255)
                                                            aaaaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCCCCAACCCCACCCAAT‑TCACCACAAACCCCCGCCCCCCGGCCATATTACACCGCGCt                                                                                            >  2:1554010/1‑151 (MQ=255)
                                                              aaTGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCTCCCCCCGCATAT‑GCCCCCCATCCACCCCCCAGCCCGCCCTATTACACGGCGCgtt                                                                                          >  3:232009/1‑151 (MQ=255)
                                                                tGTTCGCCGACAATATACTCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCACCACGCCAACCCCAACAAAT‑GCACCCAAAACACCCCACAGCCGTCCAAAATACACGGCGCTTTc                                                                                         >  4:1366635/1‑150 (MQ=255)
                                                                 gTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAg                                                                                       <  3:1343311/151‑1 (MQ=255)
                                                                    cGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCACGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGcc                                                                                     <  4:1137146/150‑1 (MQ=255)
                                                                    cGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCCTATTACACGGCGCGTTCAGccc                                                                                    >  5:1058950/1‑150 (MQ=255)
                                                                        gACAATATACGGTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTgg                                                                                <  2:1238384/151‑1 (MQ=255)
                                                                          cAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCg                                                                              <  1:846952/151‑1 (MQ=255)
                                                                              ataCGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGAc                                                                           <  8:1084658/150‑1 (MQ=255)
                                                                              ataCGCTTCCGGCTGGGTGTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGAc                                                                           <  7:215900/150‑1 (MQ=255)
                                                                                  gCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCTCCCCCACCATAT‑GCACCACAACCACCCCCCCGCCGTCCATATTACACGGCCCGTTAAGCCAGTGGCGGGCAATa                                                                       >  3:1243101/1‑150 (MQ=255)
                                                                                  gCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCG‑CCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACt                                                                     <  7:1490441/151‑1 (MQ=255)
                                                                                     tCCGTCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGTCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATCTTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTg                                                                    <  8:21065/150‑1 (MQ=255)
                                                                                      ccGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTc                                                                  <  3:67461/151‑1 (MQ=255)
                                                                                      ccGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACAACCAGCCGCCAGCCGGCCATATTCCACGGCGCGTTCAGCCAGTGGCGGACAATACTggg                                                                  >  6:660952/1‑149 (MQ=255)
                                                                                        ggCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATAT‑GCACCCCATCCAGCCGCCAGCCGTCCATATTCCACGGCGCGTTCAGCCAGTGGCGGACAATACTGTCtt                                                                >  1:579884/1‑151 (MQ=255)
                                                                                        ggCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCAGCATAA‑GCACCACATCCACCCGCCCGCCGTCCATATTCCACGGCGCGTTCAGCCAGTGGCGGCCAATACTGTCtt                                                                >  5:528071/1‑151 (MQ=255)
                                                                                        ggCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCtt                                                                <  4:926874/151‑1 (MQ=255)
                                                                                               gttctttcGCCGCTTCGGGGATCCCGGCAACGTGCGGCATATTATTGCGCGCCCCACTCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCcgcg                                                         <  2:611807/150‑1 (MQ=255)
                                                                                                ttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCcgcg                                                         <  1:168298/150‑1 (MQ=255)
                                                                                                ttctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCcgcg                                                         <  5:899098/150‑1 (MQ=255)
                                                                                                 tctttcGCCGCTTCGGTGATCCCTGCAACGTGCTGCATATTATTGCGAGCCCCACCCGCCACCCCCACCATAT‑GCACCAAATCAAACCCCCACCCCTCCATATTCCAAGGACCTTTAAGACAGTGGAGGCCAATACAGTCTTCCCCGCGa                                                        >  4:80649/1‑150 (MQ=255)
                                                                                                 tctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCAGCCCCCCCCTAT‑GCACCACAAACACCCCCCCACCGGCCATATTACACCGCGCGTTAACCCAGTGGCGGACAAAAAAGTCATACCCGCGa                                                        >  6:456998/1‑150 (MQ=255)
                                                                                                 tctttcGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGa                                                        <  5:1006557/150‑1 (MQ=255)
                                                                                                      cgccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCGCCCCCCCCATATGGCCCCCCAACC‑GCCCCCCCCCCGACAAATTACACGGCGCGTTGAGCCACTGGGGGACAAAACAGTGGTGCCCGCGCTAAAtt                                                  >  4:504655/1‑151 (MQ=255)
                                                                                                      cgccgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAtt                                                  <  2:648140/151‑1 (MQ=255)
                                                                                                      cgccgcCTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCGTAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAAtt                                                  >  3:913941/1‑151 (MQ=255)
                                                                                                         cgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCAGCATAT‑GCACCACATACAGCCGCCCGCCGGGCATATTACACGGCGCGTTCAGCCAGTGGCGGACAATAATGTGATCCCCCCGATAAAttta                                                >  6:1298935/1‑149 (MQ=255)
                                                                                                         cgcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑CCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttca                                               <  2:1265619/151‑1 (MQ=255)
                                                                                                          gcTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATttcat                                              <  8:886274/151‑1 (MQ=255)
                                                                                                           cTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCGCCCCCCCCCGCCCCCAGCAGAT‑GGACCCCATACCCCCCCCCGCCGGGCATATTACCCCGCGCGTTGAGCCAGGGGGGGGCAATAAGGTGGTGCCCGCGGTAAAAttgatt                                             >  8:1116642/1‑151 (MQ=255)
                                                                                                             gcGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATTCTGTCTTCCCCGCGATAAATttcattca                                           <  2:427466/150‑1 (MQ=255)
                                                                                                              cGGTGATCCCGGCAACGTGCGGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCGAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCAc                                          <  1:1260686/151‑1 (MQ=255)
                                                                                                                 tGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATACAGCCGCCCCCCGTCCATATTCCACGGCGCTTTAAGCCAGTGGCGGACAAAACTGTCTTGCCCGCGATAAATTTAATTAACCac                                       >  6:815392/1‑150 (MQ=255)
                                                                                                                 tGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCAACCGCCTGCCCCCACATAT‑TAAACACATCAAGCCCCCACCCCTGAAAATTACACCGCGCATTCAGCCACTGGCGGACAAAACTGTGGTGCCCGAGAAAAAATTCATTAACCa                                        >  8:12986/1‑150 (MQ=255)
                                                                                                                  gATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAg                                       <  5:723411/150‑1 (MQ=255)
                                                                                                                    tCCCGGCAACGTGCTGCATATTATTGCGCGCCCCCCCCCCCCGCCCCCCCCTAT‑GCACCCCAACCAGCCGCCACCCGGGCCAATTACCCCGCCCGTTGAGCCAGTGGCGGGCAATACAGGGGTCCCCCCGCTAAATTTGATTAACCAGAcc                                    >  3:1025719/1‑150 (MQ=255)
                                                                                                                     cccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACt                                    <  7:848552/150‑1 (MQ=255)
                                                                                                                      ccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGTCAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACttt                                  <  1:618376/151‑1 (MQ=255)
                                                                                                                      ccGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACttt                                  <  7:1384910/151‑1 (MQ=255)
                                                                                                                        ggCAACGTGCTGCATATTAATGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTcc                                <  8:338194/151‑1 (MQ=255)
                                                                                                                         gCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCg                               <  3:112270/151‑1 (MQ=255)
                                                                                                                          cAACGTGCTGCATATTATTGCGCGCCCCCCCCGCCTCCCCCCGCATAT‑GCACCCCAAACAGCCGCCCGCCCGCCATATTACACCGCGCGTTCAGCCAGTGGCCGCCCAGACTGTCTTACCCGCGCAAAATTTCATTCACCACACTATcccc                              >  5:14121/1‑149 (MQ=255)
                                                                                                                          cAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTAATTCACCAGACTTTCCg                               >  1:908090/1‑150 (MQ=255)
                                                                                                                           aaCGTGCTGCATATTATTGCGCGCCCCCCCCGCCGCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCGTTCAGCCAGTGGCGGGCAATACGGGCTTCCCCGCGATAAATTTCATTCACCAGACGTTCCGAc                             >  2:95324/1‑151 (MQ=255)
                                                                                                                            aCGTGCTGGATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACt                            <  7:942493/151‑1 (MQ=255)
                                                                                                                              gTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGa                          <  7:651288/151‑1 (MQ=255)
                                                                                                                               tgctgcATATTATTGCGCGCCCCACCCGCCTCCCCCCGCATAT‑GCCCCACATCCCGCCGCCCGCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGCCAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACGTTCCGACTGAt                         >  5:15118/1‑151 (MQ=255)
                                                                                                                                gctgcATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATa                        <  1:977025/151‑1 (MQ=255)
                                                                                                                                   gcATATTATTGCGGGCCCCACCCGCCTCCGCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCAGCAGACTTTCCGACTGATAATc                     <  3:1422796/151‑1 (MQ=255)
                                                                                                                                     atatTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCa                   <  3:1475122/151‑1 (MQ=255)
                                                                                                                                      tattatTGCGCGCCCCCCCCGCCTCCCCCCGCATAT‑GCACCACATACAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCCATAAATTTCATTGACCAGACTTTGCGACTGATAATca                    >  2:174270/1‑148 (MQ=255)
                                                                                                                                         tattGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCt                <  3:591125/150‑1 (MQ=255)
                                                                                                                                          attGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTc              <  2:899932/151‑1 (MQ=255)
                                                                                                                                           ttGCGCGCCCCACCCCCCTCCCCCAGCATAT‑GCACCACATCCACCCGCCAGCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGCCAATACTGTCTTCCCCGCGATAAAATTAATTAACCACACTTTACGACTGATAATACAGCtt               >  1:1017306/1‑149 (MQ=255)
                                                                                                                                            tGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCg             <  5:1568753/150‑1 (MQ=255)
                                                                                                                                             gcgcgcCCCACCCGCCTCCCCCAGCATAT‑GCACCAGATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCAGCAGACTTTCCGACTGATAATCCAGCTTCGGc           <  1:1496385/151‑1 (MQ=255)
                                                                                                                                               gcgcCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCaa         <  7:1143097/151‑1 (MQ=255)
                                                                                                                                                cgcCCCCCCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTACACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCaac        >  4:1174985/1‑150 (MQ=255)
                                                                                                                                                cgcCCCACCCGCCGCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGGCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAg        <  6:1214529/151‑1 (MQ=255)
                                                                                                                                                 gcCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGc       <  2:703084/151‑1 (MQ=255)
                                                                                                                                                  ccccACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCt      <  2:1498338/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
GCCACGATAGTTCATGGCGGCATCTTCCACATCGGCCTGTAACCATTGCCGTGCATCGCCAAAATGTTCGCCGACAATATACGCTTCCGGCTGGGTTTCTTTCGCCGCTTCGGTGATCCCGGCAACGTGCTGCATATTATTGCGCGCCCCACCCGCCTCCCCCAGCATAT‑GCACCACATCCAGCCGCCAGCCGTCCATATTCCACGGCGCTTTCAGCCAGTGGCGGACAATACTGTCTTCCCCGCGATAAATTTCATTCACCAGACTTTCCGACTGATAATCCAGCTTCGGCAAGCTGGCA  >  DH1/3447253‑3447553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑