Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,906,8370CG33.3% 104.2 / 19.6 76intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base C (30/16);  new base G (23/0);  total (59/17)
Fisher's exact test for biased strand distribution p-value = 6.43e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGGTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTT  >  DH1/3906687‑3906987
                                                                                                                                                       |                                                                                                                                                      
ggccccgccaGTACCCCCCCTCCGACCTACAACCTCTTTTATATTCCTGTCAGTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGc                                                                                                                                                        <  3:1298135/142‑1 (MQ=14)
 gggcggcctcgacgcccccTCGGACCAATAACCACGTTGATATTGCTCTCCCTTGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCg                                                                                                                                                       <  7:334946/137‑1 (MQ=18)
  gtgTTCAACTCACCCGCCTCGGACCTAAAAACACTTTTATATTTCTCTCACTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGa                                                                                                                                                      <  8:1400770/149‑1 (MQ=17)
     cccaactccggcgccTCGCAGCAACCACGACTTTGATAGTTCTCCCCGTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGatat                                                                                                                                                   <  4:695692/138‑1 (MQ=14)
       caactcctgccccTCGCACGAATAAAGACGTTTATATTTCTGTCTCTTTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGatat                                                                                                                                                   <  8:1315493/140‑1 (MQ=14)
                  ccTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCCCACCAAATAACGGGACATCcaaaaatgattc                                                                                                                                      >  4:903164/1‑145 (MQ=18)
                    gcccACCAACAAACTCGTTTATATTCCTGTGTCTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGAt                                                                                                                                    <  7:1325149/148‑1 (MQ=17)
                     cGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAAAGACGATAAATCAAAAAAAGTAAAAt                                                                                                                                    >  4:770864/1‑150 (MQ=18)
                      gCACCAAAAACCCCTTTTATATTGCTCGCCCTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGa                                                                                                                                  <  7:859678/151‑1 (MQ=255)
                      gCACCAAAAAACACGTTGATATTTCTCGCACTGGGCGAAGGTGGCGGTATTTGTAGACGCGCTAGCGTCAGGTGTTAGTTTTCTTACGGACGTGGGGGGTGAAATGCCCCCCCCCGCCCCCAAAGAGGGGAAATCaaaaaaatttttttgg                                                                                                                                  >  4:1519511/1‑142 (MQ=255)
                       cACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCCCCCCAAAAGAGTGTAGATCCAATAAAGTatcatgc                                                                                                                                  >  4:1533768/1‑144 (MQ=18)
                       cACCAAAAACCACGTTGATATTGCTCGCACTCGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAAACACGCGAGATCAAAAAAACTAAAAtca                                                                                                                                  >  4:1273028/1‑148 (MQ=18)
                        aCCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAAAAAAGTTAAATAAAAAAAAGTTAAATGaat                                                                                                                                >  8:825688/1‑149 (MQ=17)
                        aCCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGGTGAAGTACCCCCCCGCGCACCAAAACAGGGGATTTACAATAAAGTTTGATGACt                                                                                                                                >  7:70937/1‑151 (MQ=18)
                          cAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTAAAGTACCCCCCCAACCACCAAAAAAAGATAAAAAAAATAAAGTAAGATGaattt                                                                                                                              >  2:499780/1‑147 (MQ=17)
                          cAAAAACCACGTTGAAATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAAACGGTATATCAAATAAAGTTAGATGAgtgt                                                                                                                              >  8:1000255/1‑151 (MQ=18)
                           aaaaaaCACTTTGATATTGATCGGTCTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACtgtg                                                                                                                             <  3:512471/151‑1 (MQ=255)
                             aaaCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAACGGGAAATCCATAAAAGTTAgttccatgtttt                                                                                                                           >  8:504880/1‑140 (MQ=17)
                               aCCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTAAAGTCCCCCCCCGCGCCCCAAACTAGGGTATATCAAAAAAAGTTATTTGACTTTGTGat                                                                                                                         >  7:706247/1‑150 (MQ=18)
                                  aCGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCACCAAAAGAGGGGATTTCAAAAAAAGTTAGTTGACGTTGAGAAGGt                                                                                                                      >  7:76273/1‑151 (MQ=18)
                                      tGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAGTCGTGAAATCAATAAAAGTTTGTTGACtgtttgttgttggcg                                                                                                                  >  7:1322751/1‑139 (MQ=17)
                                        atatTGCTCGCAGTTTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGTGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGa                                                                                                                <  1:538662/151‑1 (MQ=255)
                                         tattattgttccTGGGCGAAGGTGGCTGAATTGGTAGACGCGCTTGCTTCAGGTGTTAGTGGTCTTACGGAGGTGGGGGTGCAAGTCCCCCCCCTCGCACCAACCGCGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGa                                                                                                                <  5:868941/139‑1 (MQ=21)
                                         atgatttgtatgtttgCGAAGGTGTGGTAATTTTTAGACGACATAGCTTTATGTGTAATTGTTCTTACGGACGTGGTGGTGCAATTCACACCCCTCGGACCAACCGATCCGATATCAAAAAAAGTAATATGACTGTGCGAAGGTGGCGGa                                                                                                                <  8:1298842/135‑1 (MQ=255)
                                          atTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGGTAAAGTCCCCCCCCGCGCCCCAAAACACGGGATATCAAAAATAGTTAGGTGCCTGTTGTTAGGTGGCGGAAt                                                                                                              >  1:1538775/1‑151 (MQ=17)
                                            acctccaagttagCGAAGGGCGCGGAATTGGTAGACCCTCTAGCTTCAGGTGTAAGTGTTCTTACGGACGTGTGGGTGCAAGTCCCTCCCCTCGCACCAAACGCGGCGAAATCAAAAAAAGTAAGCAGAGTGTGCGAAGGTGGCGGAAtt                                                                                                             <  8:1209670/138‑1 (MQ=255)
                                               tCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTAAAGTCCCCCCCCCACCCACCAAAACCGGGGCTAACAAAAAAAGTTATATCACTGTTGGAATGTTGCGCAGTTgtt                                                                                                          >  7:1325024/1‑149 (MQ=9)
                                                  atgtactcGAAGGTGGAGGAATTGGTAGACGCGGTAGCTTTTGGTTTTTTTGTAATTATTGACGTTTGGGTGCAAGTCCCTCGCCTGGGACTAACCGCTTCGATATCTTTTAAAGTAATTTGTGTTTGCGAAGGTGGCGGAATTGGTAGa                                                                                                       <  6:1130919/143‑1 (MQ=255)
                                                   aCTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAAAAAGTAAAAAACAAAAAGAGTAATTTAACTGAAAGAAGGTCCCCGAATTGGTataa                                                                                                      >  6:19002/1‑147 (MQ=11)
                                                    cTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAACACGGGATATCAAAAAAAGTTTGATGACTGTGGGTAGGTGGCGGAATTCGTataatc                                                                                                    >  7:1097330/1‑146 (MQ=17)
                                                      gggCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAAAACGGGAAATAAAAAAAAGTATGATGAATGTTATATCATGGCGGAATTggattacaaa                                                                                                   >  8:1124945/1‑142 (MQ=14)
                                                          gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAg                                                                                                                                      <  2:835283/111‑1 (MQ=255)
                                                           aaGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCACCAAAACAGGCGATATCAAAAAAAGTTTGATGCATGTGGTTTGGTGGCTGAATTGGTTGAAGGGGTAGCt                                                                                             >  7:244611/1‑151 (MQ=14)
                                                            aggggccGGAAGTGGGAGACGGTGTAGCTTCAGGTGATAGTGTTCTTCCGCACGGGGTGGTTGACTTCCCCGCCCTCGCACCAACCTCCGATATATCAAAAAAAGTAATCTGACTCTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCtt                                                                                            <  7:353080/145‑1 (MQ=255)
                                                            aGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAAAGGATATATCAAAAAAACGAAGATTACTGTAAGAAGGTGGCGGAATTGGTAGaacgccttggtt                                                                                            >  4:1247010/1‑140 (MQ=17)
                                                                     aaatgctacacccgcTAGCGGCAGTAGTTAGTGTTCATCGGGCCGTGGGGGTGGCAGTCCCCCCCCTCCCCCGACCCCACCCGATCTCAATCAAATTCATATCACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTa                                                                                   <  7:384799/139‑1 (MQ=9)
                                                                                 cgcTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGTATATCAAAAAAAGTTTGATGCATGTTATATGGTGCCGGAATTTATATAAGAGGAATCTTCATGTGTTActgtaaatacgc                                                                       >  5:973063/1‑143 (MQ=9)
                                                                                  gcTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCACGCCCCAAACGAGGGGATATCATAAAAAGTAAGTTGCATGTTCTAAGGTGGCGGCATTGGTAGAAGCACTAGCTGCAGGTGTGAgtgtacatatggc                                                                      >  3:375006/1‑142 (MQ=18)
                                                                                    tAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTGAAGTCCCCCCCCGCGCCCCAAAAGATGGGAAATCAAAAAAAGTTAGATGACAGTTGGTAGGTGGCGGAATTGCTAGACAGGCTAGCATCATGTGTTAgtgtaagaaaggacc                                                                    >  7:56849/1‑140 (MQ=255)
                                                                                    tAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGCGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTg                                                                                                                     >  3:299526/1‑102 (MQ=255)
                                                                                    tAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTg                                                                                                                     <  4:301743/102‑1 (MQ=255)
                                                                                            ggTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGGGAGTTCAAAAAATGTTTGATGAGTGTGGGATGGTGGCGGAATTGGTTGAAGGTGTAGCTTCATGTGTTAGTGTAATTATGGCCGTGGGGGtg                                                            >  3:121610/1‑150 (MQ=16)
                                                                                                ttAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGATATCAAAAAAAGTTAGATGCCTGTGCTTAGGTGGCGGAATTGGTATACTGGGGATCTTCAGGTGGTAGTGTCCTTAAGGCCGTGGGGGttga                                                          >  7:490993/1‑147 (MQ=25)
                                                                                                    tgtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGATTTCAAAAAAAGTAAGATGACTGTGGGAAGGTGGGGGTATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTAGGGACGTGGGGGTTGAAGTcc                                                     >  5:716741/1‑150 (MQ=37)
                                                                                                    tgtTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGGGATATCAATAATAGTTTGATGCCTGTGTTTTGGTGGCGGATTTGGTATACGGGGTAGCTTCAGGTTTTACTGTACTAAGGGCCGTGGGGGttgtaggca                                                     >  5:771163/1‑143 (MQ=16)
                                                                                                        cTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAAGGACGGGATATCAAAAAAAGTTTGATGCGTGTGGGTAGGTGGCGGATTTGGATGCCGGGGGAGCGTCAGGTGTTCGTGTAATTAGGGCCGGGGGGGtggtaggccgcccc                                                >  2:467832/1‑138 (MQ=12)
                                                                                                         ttACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAAGAGGGGAAATCAAATAAAGTTAGATGCCTGAGAGAAGGTGGCGGAATTGGTAAACGCGGGATGGTCCGGTTTTAGTGTCCATAAGGACGTGGGGGGGGAAGTcccccccc                                               >  4:514952/1‑151 (MQ=19)
                                                                                                             ggACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAAACTAGGCGATATCAAAAAAAGTAAGATGACTGTTGGAAGGTGCCGGAATAGGTAGACTAGCTAGCTGCAGGTGTTAGTGTCAATACGGACGTTGGGGTGGAAGTccaccccaagcc                                           >  1:1070240/1‑146 (MQ=25)
                                                                                                              gACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAAGGAGGCGAGATCAAAAAAAGTAAGATGACTGTGGGAAGGTGGCGGAATTGGTAGAAGGGCTAGCATCAGGTGTTAGTGTCGTTACGGACGTGGGGGTTGTAGTcaacgccatggcc                                          >  3:289762/1‑139 (MQ=25)
                                                                                                                        ttCAAGTCCCCCCCCTCGCACCAAAGGAGGGGATATCAAAAAATGTATGATGAGTGTTGGATGGTGGCGGAATGGGTTGCAGGGGGATCTGCAGGTGTTAGTGTCGTTAGGGCCGGGGGGCTGGTAGGACCCCCCCTGGCCCCAATTAttt                                >  3:938294/1‑149 (MQ=255)
                                                                                                                         tCAAGTCCCCCCCCTCGCACCAAACGAGGGGATATCAAAAAAAGTAAGATGACTGTGGGTAGGTGGCTGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTTCGGACGTGGGGGTTGAAGTCCCCCCCCAGGCCCCaagtgtgtt                               >  7:1004008/1‑144 (MQ=21)
                                                                                                                                ccccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATCTTttcttc                         >  7:376525/1‑151 (MQ=255)
                                                                                                                                 cccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCACCCCCAAGCCCCAATTATCTTTACTTcc                        >  1:605814/1‑151 (MQ=255)
                                                                                                                                  ccccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGCTAGACGCGCTATCTTCAGGTGTTAGTGTCCTTACGGACGTGGAGGTTAAAGGACCCCCCCTAGCAACAAATATCttaaagtac                        >  2:1490655/1‑143 (MQ=21)
                                                                                                                                   cccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCtt                      >  3:726008/1‑151 (MQ=255)
                                                                                                                                    ccccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTGAAGGCCCCCCCCGCGCCCCAATTATGTTTACTTCCgtt                     >  5:62700/1‑151 (MQ=21)
                                                                                                                                      ccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCt                   >  4:907825/1‑151 (MQ=255)
                                                                                                                                      ccTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTAAAGTCCCCCCCCGCGCCCCAATTATGTTTAATTCCattat                   >  6:28130/1‑149 (MQ=21)
                                                                                                                                        tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTg                 >  3:710201/1‑151 (MQ=255)
                                                                                                                                        tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTg                 >  4:135052/1‑151 (MQ=255)
                                                                                                                                        tCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTACAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTg                 >  2:1189070/1‑151 (MQ=255)
                                                                                                                                          gCACCAAACGAGTCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTAAGGACGTGGGGGTTAAACTCCCCCCCCGCGCACCAAATATCATTACTTACTTTCTTGtt               >  8:895827/1‑151 (MQ=21)
                                                                                                                                          gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGtt               >  5:500994/1‑151 (MQ=255)
                                                                                                                                          gCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAGTCCCCCCCCGCGCCCCCATTATCTTTACTTCCTTTCTtttt               >  8:23296/1‑151 (MQ=255)
                                                                                                                                              cAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCGCGCCCCCATTATCTTTACGTGCTTTCTTGTttctt           >  5:53512/1‑151 (MQ=255)
                                                                                                                                               aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTGCTTTCTtgtttgttg          >  6:141319/1‑150 (MQ=255)
                                                                                                                                               aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGGCCCCCCCCTCGCACCAATTATATTTACTTCCTTTCTTGTttattc          >  4:1487802/1‑151 (MQ=255)
                                                                                                                                               aaaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGCTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCACCCCCTCGCACCAAATATCTTTACTTCCTTTCTTGTttcttc          >  6:1260209/1‑151 (MQ=255)
                                                                                                                                                aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGTCCCCCCCCGCGCCCCAATTATGTTTTATTACTTTGTTTTTTTTTcc         >  3:1541622/1‑151 (MQ=18)
                                                                                                                                                aaCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCGCCCCCGCGCACCAATTATATTTTCATCCTTTGGTGtttggtc          >  8:552086/1‑146 (MQ=18)
                                                                                                                                                 aCGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTGAAGTCCCCCCCCTCGCCCCAATTATCTTTACTTCCTTTCTTGTTTCTTGCt        >  6:1334965/1‑151 (MQ=255)
                                                                                                                                                  cGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATGTTTACTTCCTTTCTTGTTTGTTCGtt       >  4:905813/1‑151 (MQ=255)
                                                                                                                                                  cGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGGTTCTTCCtt       >  4:320082/1‑151 (MQ=255)
                                                                                                                                                   gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCCAGTCCCCCCCCGCGGCCCAATTATCTTTTCTTCCTTTCTTGTTTCTTGCttt      >  5:882345/1‑150 (MQ=21)
                                                                                                                                                   gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTAtggtt                              >  4:244229/1‑123 (MQ=255)
                                                                                                                                                   gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCttt                              <  3:242181/127‑1 (MQ=255)
                                                                                                                                                   gAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTGTTGTTTcttgcttg      >  6:1161481/1‑151 (MQ=255)
                                                                                                                                                       cGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCACGTCACCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGAttt  >  2:1102769/1‑151 (MQ=255)
                                                                                                                                                       cGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCCCAATTATGTTTACTTCCTTTCTTGTTTGGTCCTTGAttt  >  1:988973/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
GGGGGTTCAAGTCCCCCCCCTCGCACCAAAAACCACGTTGATATTGCTCGCACTGGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAAACGAGGCGATATCAAAAAAAGTAAGATGACTGTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCAATTATCTTTACTTCCTTTCTTGTTTCTTCCTTGATTT  >  DH1/3906687‑3906987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: