Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,122,1240CG23.4% 309.9 / 38.8 245intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (92/59);  new base G (43/3);  total (178/67)
Fisher's exact test for biased strand distribution p-value = 9.27e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

TCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121976‑4122272
                                                                                                                                                    |                                                                                                                                                    
      aaCTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTAGTCACGCGTGGGTGGTCTGTAATTTggagc                                                                                                                                              >  2:1379992/1‑151 (MQ=14)
          gAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGGTTTGTTGGTCTTTGtttatgagtaatt                                                                                                                                          >  3:1412929/1‑141 (MQ=14)
           cccaaacccccgggggggggGCTGTCCATTAAAGCACTTTAACATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACttt                                                                                                                                         <  7:688862/137‑1 (MQ=14)
            gCTTTTCGCGCTTGGGGGGTCTGTGCCTTTCAGGACTTTCATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACttt                                                                                                                                         <  4:512378/150‑1 (MQ=17)
             catatCCCGCTTGGGCGCTCTGTCCCTTACAGCCCGTGCATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTc                                                                                                                                       <  7:1239593/147‑1 (MQ=17)
             cTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTTGGTGGTCTTTGTTTTGCAGCACTTTTc                                                                                                                                       >  7:115203/1‑151 (MQ=255)
              tATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGCTGTGCCGCACTTTTcc                                                                                                                                      >  1:98918/1‑150 (MQ=255)
              aattcccaggtGGGGGGTCTGTACCTTATAGATCTATAATAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCa                                                                                                                                      <  3:536911/141‑1 (MQ=14)
               aTTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTGCCGCGTGGGTGGTCTGTGCTTTGCAGCACTTTTCa                                                                                                                                      >  7:537729/1‑150 (MQ=255)
                ttCCGGGTTGGGTGGTGTGTTCCTTAGATGACTTTCATATTTTGGAGCGGGAAACGAGACTGGATGTCGCGACCCCGACCTTGGCAAGGTCGTGGTCTACGTACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTGaa                                                                                                                                     <  8:331996/150‑1 (MQ=17)
                aacgcGCCTGGGCGGTCTGTCCATTACATCCCTAGAATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCaaa                                                                                                                                    <  4:1171607/147‑1 (MQ=14)
                 tCCCGCGTGGGTGGTCTGTCCCTTACAGCACTTTCACATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCaaa                                                                                                                                    <  3:785206/150‑1 (MQ=255)
                 accccCTTCGGGGGTCTGGCCATTTCATCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAAt                                                                                                                                   <  5:728612/150‑1 (MQ=17)
                   ccGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCTTGGTATGTGGTTTGAAGGAATTTTCAAAttt                                                                                                                                 >  4:1490/1‑151 (MQ=17)
                   ccGCGTGGGCGCTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAAttt                                                                                                                                 <  3:240023/151‑1 (MQ=255)
                    cGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTTGTTTGAAGCACTTTTCAAAtttt                                                                                                                                >  6:7563/1‑151 (MQ=255)
                    cGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTCGTCTGTGATTTGCAGGACTTTTCAAAtttt                                                                                                                                >  6:1234002/1‑151 (MQ=255)
                      gggggggggtgggggCCTTACAGCACTAGAAAATTTTGGAGGGGGAAACGAGACGCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTgg                                                                                                                              <  7:832971/137‑1 (MQ=14)
                      cTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGATTATCCGCACTTTACAAATTTTgg                                                                                                                              >  7:1091095/1‑151 (MQ=17)
                       ttGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGAGGTCATTTATATGGAGCAATTTTCCAAtttgtgc                                                                                                                             >  5:989771/1‑147 (MQ=14)
                        tGGGTGGTCTGTGCCTTACACCACATTCAAATTTTGCAGCGGCAAACGAGACTCCAACTCGCGACCCCGACCATGGCAAGCTCGTGCTCTACCAACTGATCTATTCCCCCATCCGTCCTCTCTGCTATGACGCACTTTTAAAAgttagc                                                                                                                              >  6:1498997/1‑146 (MQ=255)
                         gggTGGTGTGGTCATTTCATCTCTTTCATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGc                                                                                                                           <  2:177894/151‑1 (MQ=14)
                         gggTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGTTTTGGAGGACTTTTCAAATTTTGGAgg                                                                                                                           >  1:1511671/1‑150 (MQ=255)
                         gggTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTGGTCTGTGCGTTGGAGGACTTTTCATAGTTTGGAGc                                                                                                                           >  3:656701/1‑151 (MQ=17)
                          gggggtGTGTGCATTACATCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCg                                                                                                                          <  1:747605/148‑1 (MQ=255)
                           gtggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGGGTGCAGGACTTTTCTAATTTTGGAGCgg                                                                                                                         >  5:408957/1‑151 (MQ=255)
                             ggtCTTTGCCTTACAGCACTTTCAAATTTTCTAGCGTGAAACGAGACACTACATCGCGACCCCGACCTTGGCAAGGTAGTTATCTACCAACTGATCTATTAACGCTTTGTAGGTATTTGCTATGTAGAAATTTTCAAATTTTGGAggcgg                                                                                                                        >  2:986614/1‑146 (MQ=12)
                               tCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCTTGGTATGTGATGTGCCGCAATTTTCTAATTTATGAGCgggtac                                                                                                                     >  2:649366/1‑148 (MQ=14)
                               tCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAAGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGGTGTGACGCGCTGTTAAAATTTTGGCGCGGCaa                                                                                                                      >  7:750357/1‑150 (MQ=17)
                                 tgtgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTAGGAGTTCTTTTATGTGACGGAATTTACGAATTTTTGAGGGGTAAACg                                                                                                                   >  5:966699/1‑151 (MQ=14)
                                   tgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTGCTGTGTAGCACTTTTCTAATTTAGGAGAGGGAAACg                                                                                                                   >  1:1400788/1‑149 (MQ=17)
                                    gCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTTGTTTTCAGCACTTTTCAAATTTTTGAGCGGGAAACgagc                                                                                                                >  1:114182/1‑150 (MQ=255)
                                         aCAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGTTATGTTGTTTTTAGTAATTTTCTAATTTTTGAGAGGGaacggcgcctcg                                                                                                            >  1:604720/1‑140 (MQ=14)
                                          cAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGTTGGTCTGTGCTTTGAAGGAATTTTCAAATTTTGGAGCGGGAAACGAGACGCGa                                                                                                           >  4:82320/1‑150 (MQ=17)
                                          cAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGAGGTATGTGGGATGAAGCACTTTTCAAATTTTTGAGAGGGAACAGAGCCTCGa                                                                                                           >  3:198066/1‑150 (MQ=17)
                                                    aaaTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTGTGCCGGAATTTTCTAATTTTGGAGCGGGAAACGAGCCTCGATCTCGCGAccc                                                                                                >  1:267904/1‑151 (MQ=14)
                                                     aaTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGGTCTGTGCTTTGCAGAACTTTTCAAATTTTGGAGAGGGAAACGAGACTCCAAATACCGAcca                                                                                                >  2:1319384/1‑149 (MQ=14)
                                                     aaTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTCGTCTTTTATTTGAAGAAATTTTCAAATTTTTGAGAGGGTAACGATAGTCCAAATAGCGAcaac                                                                                               >  4:1001337/1‑148 (MQ=14)
                                                      aTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCCGTCGTATGTGGTATGAAGAAATTTTCAAATTTTTGAGAGGGAAACGAGCCTCGAACTAGCGAccccc                                                                                              >  8:775509/1‑150 (MQ=14)
                                                          tGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTGTTTGCAGGACTTTTCTAATTTTGGAGCGGTAAACGAGCCTCGAAGTCGCGACCCAGCCCt                                                                                          >  1:501738/1‑151 (MQ=14)
                                                             aGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGAGGGAACCGAGCCTCGAACTCGCGACACCGACCTTgg                                                                                       >  5:1399700/1‑151 (MQ=255)
                                                              gCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGAGGTATGTGCTTTGAAGCAATTTTCAAATTTTGGAGCGGGAAACGAGACTCGATCTCTCGACCACGACCTTCGc                                                                                      >  6:652062/1‑151 (MQ=14)
                                                               cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATGTGCAGAACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTGGGc                                                                                      >  8:1174446/1‑150 (MQ=255)
                                                               cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTTATTTGGCGGACTTTTCTAATTTTGGAGAGGGAACCGAGGCTCGAACTAGCGACCCCGAGCTTggg                                                                                      >  7:1503440/1‑149 (MQ=14)
                                                                 ggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTATGTGTTGTGGAGGACTTTTCTAATTTTGGAGCGGGAAAGGCGCCTCGCACTCGCGCACCAGCCCTTGGctat                                                                                   >  5:498442/1‑148 (MQ=14)
                                                                    aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTCTTTGAAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAAATCGCGACCCCGAGCTTGGCAAGGTc                                                                                >  2:1437901/1‑151 (MQ=255)
                                                                     aagagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGATATTACAGCATCGTTCGTATGTGATTTTAAGAACTATTCAAATTTATGAGCCGGAAACGAGACACGAACAACCGACACCGACCtagcaaagct                                                                                 >  6:3254/3‑140 (MQ=2)
                                                                      cGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGTTGGTCTGTGATTTGACGAACTTTTCAAATTTTTGAGCGGGAACCGAGACTCGAAATAGCGACCCCGACCTTGGAAAGGTCgg                                                                              >  6:936547/1‑150 (MQ=14)
                                                                      cGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGTTGGTATGTGGTGTGGAGAAATTTTCTAATTTTGGAGGGGGAAACGCGACTCGAACTCCCGACCCCTACGTTGGCAAGGTCgg                                                                              >  1:873565/1‑150 (MQ=14)
                                                                       gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGATCTCGCGACCCCGACCTTGGGAAGGTCGtc                                                                             >  8:552678/1‑150 (MQ=255)
                                                                       gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGGTCTGTGCTTTGACGCACTTTTCAAATTTTGGAGCGGGAAACGCGACTCGAACTCGCGACCCATCGCTTGGCATGGTCGTg                                                                             >  7:916244/1‑151 (MQ=14)
                                                                             cGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGATCTCGCGACCCCGACCTCGGCAAGGTCGTGCTCTAc                                                                       >  1:1219809/1‑151 (MQ=255)
                                                                                 cTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTATGTGCTGTGCAGAACTTTTCAAATTTTGGAGGCGGAAACGAGACTCGAACTCGCGACACCGACCTTGGCAAGGTCGTGCTCTACAAAc                                                                   >  1:1458794/1‑151 (MQ=14)
                                                                                   cgggACCCCGACCGTGGCTGGGGCGTGCCCTACCAACTGCGCTATTCCCGCGTGGGTGGGCTGTGCGTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTg                                                                 <  1:1366160/148‑1 (MQ=11)
                                                                                       aCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGTTCTGTGCTTTGAAGCACTTTTCAAATTTATGAGCGGTAAACGAGACTCGAACTCGCGACCCCGACATAGGAAAGGTCGTCCACTACAAACtt                                                                 >  8:702397/1‑146 (MQ=14)
                                                                                        ccccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCtt                                                            >  2:152852/1‑150 (MQ=38)
                                                                                         cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTTCGACGTCTTTGATATTCAGGAATTTTCAAATTTAGGAGCCGGAAACGAGACACGAACTCTCGACCCCTACCTTCCACAGCTCGAGCTCTACCAACAGCGCTAt                                                           >  6:1086620/1‑151 (MQ=2)
                                                                                          ccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTAGCGACACCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTAtt                                                          >  1:90117/1‑151 (MQ=255)
                                                                                            gcccTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTTTGCTTTGCAGCACTTTTCCAATTTTGGAGCGGGAAAGGAGACTCGACCTCGCGACCCCGACCTGGGCAAGGTCGTGCTCTACCAACTGAGCTTTTcc                                                        >  4:384300/3‑151 (MQ=14)
                                                                                            gACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGTTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGCGACGCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTcc                                                        >  5:524743/1‑151 (MQ=255)
                                                                                            caCCTTGGTAATGTCGGGCTCTACCCACTGAGGTCTTCCCCATTGGGGGGTCTGGGCGTGTCTGCTGTTTTTAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTcc                                                        <  5:1007122/150‑1 (MQ=11)
                                                                                              ccTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGCGGTAAACGAGACTCGAACTCGCGACACCGACCTGGGCAAGGTACTGCTCTAACAACTTAGCTATTCCcg                                                      >  5:544622/1‑151 (MQ=14)
                                                                                              acTTGGCTAGGTGGGTCTCTACGACGTGCGTTATTCCCTCTGGGGTGGTGTGGGATTTTAATAAGTTTTAAAATTTTGGAGCGGGAAACGAGACTCTAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCcg                                                      <  7:1387324/150‑1 (MQ=2)
                                                                                               gttGGCAATGTTGGGCTCTACGAAGTGATGTATTCCGGATGGGGTGGTTGTTGATTTTTATTATTTTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCcgc                                                     <  8:341426/150‑1 (MQ=2)
                                                                                               cTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCcgc                                                     >  5:676512/1‑151 (MQ=38)
                                                                                                    cAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGtact                                                >  5:196212/1‑151 (MQ=255)
                                                                                                       ggTCGTGCGCTACCAACTGAGCAATACCCGCTTGGGTGGGGTTGGCTTTGAAGCACTTTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTAGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTactact                                             <  7:344428/151‑1 (MQ=14)
                                                                                                         tCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTCGTCTGTTGTTTGGAGCAATTTTCAAATTTTGGAGAGGGAAACGAGCGTCGAAGTCGCGACCCCGACATACGCAAGGACGGCCGCTAACAACTGAGCTATTccatggtaggtcta                                            >  2:492247/1‑138 (MQ=9)
                                                                                                          cGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGGAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTGGCCAAGGTCCTGCTCAACCAACTGAGCTATTCCCGCTTACTACTTa                                           >  7:640099/1‑150 (MQ=14)
                                                                                                            tGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAAtt                                        >  3:1097543/1‑151 (MQ=255)
                                                                                                            tGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGGTGTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGCCCCGCGAACTGGCGACCCCGCGCTTGGCCAGCTCGAGCGCTACCAACTGAGCTATTCCCGTGTACTACAGAAtt                                        >  6:372929/1‑151 (MQ=14)
                                                                                                            ggccgtcccAACTGAGTTAGTCCCGCTTTGGTGGTGGGTGCTTTTAACCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAAtt                                        <  5:855772/144‑1 (MQ=14)
                                                                                                               tctACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTTGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTg                                     >  1:1303755/1‑151 (MQ=255)
                                                                                                               tctACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTg                                     >  3:1270525/1‑151 (MQ=255)
                                                                                                               tctACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGAGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTg                                     >  8:501275/1‑151 (MQ=255)
                                                                                                               tctACCAACTGAGCTATTCCCGCTTGGGTGGTATGTTCTTTGAAGGACTTTTCAAATTTTGGAGCGGGAAACGAGGGTCGAACTAGCGACAACGACATGGGGAAGGTCGTGCTCTACAAACTGAGCTATTCCAGAGTACGACTTAATTTTg                                     >  8:1260200/1‑151 (MQ=14)
                                                                                                               ccaacaaacccaGCAATTCCGCCTGGGGTGGGGTGGGCTATTCACCACTTATCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTg                                     <  7:241831/140‑1 (MQ=14)
                                                                                                                 tACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCt                                   >  2:723599/1‑151 (MQ=255)
                                                                                                                     aaCTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCg                               >  6:1574372/1‑151 (MQ=255)
                                                                                                                     aaCTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCg                               >  2:385404/1‑151 (MQ=255)
                                                                                                                         gAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAt                           >  5:1470390/1‑151 (MQ=255)
                                                                                                                             tATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTc                       >  4:895753/1‑151 (MQ=255)
                                                                                                                              atttcttcttttcttgtCTGTGTTTTGCAGAACTTTTCTATTTTTGGATCTGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCg                      <  6:500122/136‑1 (MQ=18)
                                                                                                                              aatCCCGCTTGGGGGGGCTGGGCCTTGCAGCAATTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTc                       <  7:1043728/148‑1 (MQ=18)
                                                                                                                              aTTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCg                      >  1:1023517/1‑151 (MQ=255)
                                                                                                                               ttCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     >  5:257969/1‑151 (MQ=255)
                                                                                                                               atgcccgaggggTGCTCTGCGGATTTCAACACATTTAAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     <  7:480638/142‑1 (MQ=18)
                                                                                                                                tCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     >  5:605651/1‑150 (MQ=255)
                                                                                                                                 cccGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAt                   >  3:941780/1‑151 (MQ=255)
                                                                                                                                 cccGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTACTTTCGTAATTTTCGCAt                   >  2:953368/1‑151 (MQ=255)
                                                                                                                                  ccGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAtt                  >  6:790157/1‑151 (MQ=255)
                                                                                                                                  ccGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAtt                  <  5:1228337/151‑1 (MQ=255)
                                                                                                                                  ccGCTTGGGTGGGGTGGGATTTTCATCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCAtt                  <  5:444341/151‑1 (MQ=18)
                                                                                                                                     gttGGGGGGGGTGTGCTTTGCAGCAGTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCt               <  7:592765/150‑1 (MQ=255)
                                                                                                                                      ttGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTg              >  2:1134584/1‑151 (MQ=255)
                                                                                                                                          gtgggttttgCTATGTATCAATTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtc          <  8:1001618/143‑1 (MQ=18)
                                                                                                                                           tggtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtcg         >  7:1315446/1‑151 (MQ=255)
                                                                                                                                             gtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGtt       >  2:455316/1‑151 (MQ=255)
                                                                                                                                                   gCTTTTCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  <  6:359843/150‑1 (MQ=255)
                                                                                                                                                    cTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  >  3:1341720/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121976‑4122272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑