Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 1,284,869 G→A G290S (GGT→AGT)  dapA → 4‑hydroxy‑tetrahydrodipicolinate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,284,8690GA96.4% 1320.0 / 21.2 362G290S (GGT→AGT) dapA4‑hydroxy‑tetrahydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base G (6/7);  new base A (151/197);  total (158/204)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 362 total aligned reads displayed.

CAACAAACTATTTGTCGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCGGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAGTGGTGATGAAGCC  >  DH1/1284721‑1285016
                                                                                                                                                    |                                                                                                                                                   
 aacaaACTATTTGTCGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGtt                                                                                                                                                  >  2:1471984/1‑151 (MQ=255)
 aacaaACTATTTGTCGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGtt                                                                                                                                                  >  2:1098486/1‑151 (MQ=255)
 aacaaACTATTTGTCGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGtt                                                                                                                                                  >  8:1510838/1‑151 (MQ=255)
     aaCTATTTGTCGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTTAGACGTGAAGAGCGGCGGTAAGGCTTGCAAGTTTGcg                                                                                                                                              >  2:1399339/1‑150 (MQ=255)
      aCTATTTGTCGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCGGTTTGCTg                                                                                                                                             >  1:1034260/1‑151 (MQ=255)
          tttGTCGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTaa                                                                                                                                          >  4:1159717/1‑150 (MQ=255)
           ttGTCGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGCAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTaaa                                                                                                                                         <  6:1572888/150‑1 (MQ=255)
            tGTCGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAg                                                                                                                                        <  3:246439/150‑1 (MQ=255)
            tGTCGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGt                                                                                                                                       >  8:1125363/1‑151 (MQ=255)
             gTCGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGtt                                                                                                                                      <  1:355197/151‑1 (MQ=255)
              gcGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGttt                                                                                                                                     <  7:1025775/150‑1 (MQ=255)
               cGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGCCCGATACGCTGCGCCTGCCAATGCCCCCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCGTAAGCATGCCAGTTTGCTGTAAAGttt                                                                                                                                     >  3:1500459/1‑150 (MQ=255)
               cGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTa                                                                                                                                    <  1:1155805/151‑1 (MQ=255)
               cGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTa                                                                                                                                    <  6:1439303/151‑1 (MQ=255)
                gAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGtat                                                                                                                                     >  4:799491/1‑147 (MQ=255)
                   cccAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAggg                                                                                                                                 >  8:322263/1‑150 (MQ=255)
                   cccAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAggg                                                                                                                                 >  8:826973/1‑150 (MQ=255)
                    ccaatccaatCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGgag                                                                                                                               <  3:394869/151‑1 (MQ=255)
                    ccaatccaatCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGATTAggg                                                                                                                                 >  7:230812/1‑149 (MQ=255)
                      aatccaatccCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGAt                                                                                                                             <  1:520847/151‑1 (MQ=255)
                         ccaatccCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTg                                                                                                                          <  5:313875/151‑1 (MQ=255)
                          caatccCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGa                                                                                                                         <  7:175453/151‑1 (MQ=255)
                           aatccCGGTGAAATGGGCATGTAAGGAACTGGGGCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACAGCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCGTAAGCATGCAAGTTTGCTGTAAAGTTTAGGGAGATTTGAt                                                                                                                        <  7:1326471/151‑1 (MQ=255)
                            atccCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATg                                                                                                                       >  5:390954/1‑151 (MQ=255)
                              cccGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGCCCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGc                                                                                                                     >  8:567965/1‑151 (MQ=255)
                                  gTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCTATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTAc                                                                                                                 >  6:1108331/1‑151 (MQ=255)
                                  gTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTAc                                                                                                                 <  7:1496649/151‑1 (MQ=255)
                                    gAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTActc                                                                                                               >  1:6972/1‑151 (MQ=255)
                                     aaaTGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTActct                                                                                                              <  4:1015733/151‑1 (MQ=255)
                                     aaaTGGGCATGTAACGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTActc                                                                                                               <  4:872002/150‑1 (MQ=255)
                                      aaTGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTg                                                                                                             >  4:1516774/1‑151 (MQ=255)
                                      aaTGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTg                                                                                                             >  2:1524179/1‑151 (MQ=255)
                                      aaTGGCCATGTAAGGAACTGGGTCTTGTGGCGCCCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCATAAGCATGCGAGTTAGCTGTAAAGTTTAGGGAGATATGATGGAGTACTCTg                                                                                                             >  6:683039/1‑151 (MQ=255)
                                         gggCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGtt                                                                                                           <  8:798462/150‑1 (MQ=255)
                                             aTGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCaaa                                                                                                       >  2:1222109/1‑150 (MQ=255)
                                               gTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCGGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGt                                                                                                    >  8:1070708/1‑151 (MQ=255)
                                                 aaGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTcg                                                                                                  <  2:452200/151‑1 (MQ=255)
                                                     aaCTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGcc                                                                                              >  5:174698/1‑151 (MQ=255)
                                                         ggggCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGTTGGCTTACTCTGTTCAAAAGTCGCGCCTg                                                                                            <  8:1214358/149‑1 (MQ=255)
                                                         gggTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTgg                                                                                           <  7:446296/150‑1 (MQ=255)
                                                            tCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCt                                                                                             <  4:197361/145‑1 (MQ=255)
                                                            gcTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCaaa                                                                                       <  7:593111/150‑1 (MQ=255)
                                                            gcTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATGTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCaaa                                                                                       <  7:1244960/150‑1 (MQ=255)
                                                               tgtgGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAgg                                                                                     <  6:493050/150‑1 (MQ=255)
                                                               tgtgGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAgg                                                                                     <  1:120392/150‑1 (MQ=255)
                                                               tgtgGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAg                                                                                      <  5:15171/149‑1 (MQ=255)
                                                                 tgGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTg                                                                                  >  5:1247495/1‑151 (MQ=255)
                                                                  ggCGACGGTTACGCTGCGGCGGCCAATGACAGCAATCAGCGAGAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGc                                                                                 <  2:313797/151‑1 (MQ=255)
                                                                  ggCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTg                                                                                  <  6:1553669/150‑1 (MQ=255)
                                                                     gACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGCCGCTTAAGCATGCGAGTTTCCTGTAAAGTTTAGTGAGATTTGATGGCTTACTCTGTTCAAAACTCGCGCCTGGCAAAGGTTGCggg                                                                              >  7:958370/1‑151 (MQ=255)
                                                                       ccGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGAGTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGgtg                                                                            <  1:899772/151‑1 (MQ=255)
                                                                        cGATACGCTGCGCCTTCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGgtgt                                                                           <  1:797191/151‑1 (MQ=255)
                                                                            aCGCTGCGCCTGGCAATGACACCAATCAGCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTc                                                                        <  6:136318/150‑1 (MQ=255)
                                                                            aCGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTc                                                                        <  1:1509920/150‑1 (MQ=255)
                                                                            aCGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCg                                                                       <  5:1297144/151‑1 (MQ=255)
                                                                                tGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTg                                                                   >  8:916570/1‑151 (MQ=255)
                                                                                 gcgcCTGCCAATGACACCAATCACCGACAGGGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCtt                                                                    <  4:330144/149‑1 (MQ=255)
                                                                                   gcCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGttt                                                                >  6:611840/1‑151 (MQ=255)
                                                                                    ccTGCCAATGACACCTAGCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGtttt                                                               <  2:618187/151‑1 (MQ=255)
                                                                                     cTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGtttt                                                               >  8:1310973/1‑150 (MQ=255)
                                                                                     cTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGtttt                                                               >  3:1163636/1‑150 (MQ=255)
                                                                                       gCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTttatt                                                            <  8:212666/151‑1 (MQ=255)
                                                                                       gCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTACGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTttatt                                                            <  6:1283603/151‑1 (MQ=255)
                                                                                        ccAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCGGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTttatta                                                           <  2:1322558/151‑1 (MQ=255)
                                                                                        ccAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTttatta                                                           <  2:947441/151‑1 (MQ=255)
                                                                                        ccAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTttatta                                                           <  6:923829/151‑1 (MQ=255)
                                                                                         cAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTAc                                                          <  8:441485/151‑1 (MQ=255)
                                                                                          aaTGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACt                                                         >  5:1093315/1‑151 (MQ=255)
                                                                                            tGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCg                                                       >  4:98786/1‑151 (MQ=255)
                                                                                              acacCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGCTGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCt                                                     <  3:1380331/151‑1 (MQ=255)
                                                                                                   aaTCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTg                                                <  5:629634/151‑1 (MQ=255)
                                                                                                      cACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCGGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAg                                             <  2:1044743/151‑1 (MQ=255)
                                                                                                        ccGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGTTTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCTAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGtt                                           <  1:711211/151‑1 (MQ=255)
                                                                                                          gACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCt                                         >  8:645466/1‑151 (MQ=255)
                                                                                                           aCAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCCGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTg                                        >  5:776278/1‑151 (MQ=255)
                                                                                                             aGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGAc                                      >  2:846471/1‑151 (MQ=255)
                                                                                                              gTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACt                                     >  4:284346/1‑151 (MQ=255)
                                                                                                                   cGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACg                                 <  6:799303/150‑1 (MQ=255)
                                                                                                                    gTGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCt                               >  1:931980/1‑151 (MQ=255)
                                                                                                                     tGAGACGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCt                               >  7:557920/1‑150 (MQ=255)
                                                                                                                       agaCGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCtata                            <  1:1318625/151‑1 (MQ=255)
                                                                                                                         aCGGTCAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAg                          <  3:1015907/151‑1 (MQ=255)
                                                                                                                           ggTCAGAGCGGCGCTTAAGCATGCCGGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCg                        <  5:1165463/151‑1 (MQ=255)
                                                                                                                              cAGAGCGTCGGTTTAGCATGCCAGTTTGCTGTATATTTTAGGGAGATTTGATGGCTTACTCTGTTCTAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCgtc                      <  6:244614/150‑1 (MQ=255)
                                                                                                                              cAGAGCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCgtc                      <  7:320391/150‑1 (MQ=255)
                                                                                                                               agagCGGCGCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTCGCAAAGGATGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCAAGCTATAAGCgtc                      >  2:786331/1‑149 (MQ=255)
                                                                                                                                 agCGGCGCTTAAGCATGCCAGTTTCCTGTAAAGTTTAGCGAGATATGATGGCTAACTCTCTTCAAAAGTCGCGCCTCGCAAACGTTGCCCGTGTATCGCTAGTTTAATAACACGCAGCCAGTAGTTCTGACTCACGCTATAAGCgtcacg                   >  4:1297535/1‑148 (MQ=255)
                                                                                                                                  gcggcgCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCgtcaggtc                 >  8:1414718/1‑151 (MQ=255)
                                                                                                                                    ggcgCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCgtcaggtcag               <  7:1140290/151‑1 (MQ=255)
                                                                                                                                    ggcgCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCgtcaggtcag               <  3:1070458/151‑1 (MQ=255)
                                                                                                                                    ggcgCTTAAGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCgtcaggtc                 >  4:606195/1‑149 (MQ=255)
                                                                                                                                      cgcTTAAGTATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAgtg             <  6:338265/151‑1 (MQ=255)
                                                                                                                                          tAAGCATGCCAGTTTGCTGTAAAGTTTAGTGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAgtggtg          <  3:794550/150‑1 (MQ=255)
                                                                                                                                          tAAGCATGCCAGGTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAgtggtg          <  3:555804/150‑1 (MQ=255)
                                                                                                                                           aaGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAGTGGtgat        >  5:876213/1‑151 (MQ=255)
                                                                                                                                            aGCATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAGTGGtgatg       >  8:1009400/1‑151 (MQ=255)
                                                                                                                                              cATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAGTGGtgatga      >  8:261227/1‑150 (MQ=255)
                                                                                                                                              cATGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAGTGGTGATGaa     <  1:986916/151‑1 (MQ=255)
                                                                                                                                                tGCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAGTGGTGATGAAGc   >  4:45987/1‑151 (MQ=255)
                                                                                                                                                 gCCAGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAGTGGTGATGAAGcc  >  4:421748/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAACAAACTATTTGTCGAACCCAATCCAATCCCGGTGAAATGGGCATGTAAGGAACTGGGTCTTGTGGCGACCGATACGCTGCGCCTGCCAATGACACCAATCACCGACAGTGGTCGTGAGACGGTCAGAGCGGCGCTTAAGCATGCCGGTTTGCTGTAAAGTTTAGGGAGATTTGATGGCTTACTCTGTTCAAAAGTCGCGCCTGGCAAAGGTTGCGGGTGTTTCGCTTGTTTTATTACTCGCTGCCTGTAGTTCTGACTCACGCTATAAGCGTCAGGTCAGTGGTGATGAAGCC  >  DH1/1284721‑1285016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: