Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,730,866 T→G I39L (ATC→CTC)  fabH ← 3‑oxoacyl‑ACP synthase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,730,8660TG97.4% 1455.4 / 16.5 377I39L (ATC→CTC) fabH3‑oxoacyl‑ACP synthase III
Reads supporting (aligned to +/- strand):  ref base T (5/5);  new base G (157/210);  total (162/215)
Fisher's exact test for biased strand distribution p-value = 7.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

Only 100 of 378 total aligned reads displayed.

CGGGAAAGCGTGCGTAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGATACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATACCGTCA  >  DH1/2730717‑2731014
                                                                                                                                                     |                                                                                                                                                    
  ggAAAGCGTGCGTAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGAcc                                                                                                                                                   <  3:409525/151‑1 (MQ=255)
   gAAAGCGTGCGTAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCg                                                                                                                                                  <  4:896639/151‑1 (MQ=255)
      aGCGTGCGTAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGt                                                                                                                                                >  6:154283/1‑150 (MQ=255)
       gcgtgcgtAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTAc                                                                                                                                              <  2:788663/151‑1 (MQ=255)
       gcgtgcgtAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTAc                                                                                                                                              <  8:120767/151‑1 (MQ=255)
       gcgtgcgtAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTAc                                                                                                                                              <  4:404689/151‑1 (MQ=255)
         gtgcgtAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGa                                                                                                                                            <  5:1467936/151‑1 (MQ=255)
          tgcgtAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACgcg                                                                                                                                           >  7:938519/1‑149 (MQ=255)
             gtAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGGTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTgc                                                                                                                                        >  4:1434058/1‑150 (MQ=255)
              tAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGAc                                                                                                                                       >  2:1208431/1‑151 (MQ=255)
               aGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGAc                                                                                                                                       >  1:520292/1‑150 (MQ=255)
                gCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACaa                                                                                                                                     <  8:859362/151‑1 (MQ=255)
                gCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACaa                                                                                                                                     <  2:518003/151‑1 (MQ=255)
                 cAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAAt                                                                                                                                    <  3:1288538/151‑1 (MQ=255)
                 cAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAAt                                                                                                                                    >  5:1273279/1‑151 (MQ=255)
                    aaGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATcc                                                                                                                                  >  6:1054203/1‑150 (MQ=255)
                     aGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCAc                                                                                                                                <  7:544559/151‑1 (MQ=255)
                          ttGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTc                                                                                                                           >  1:918159/1‑151 (MQ=255)
                           tGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTc                                                                                                                           <  7:1246264/150‑1 (MQ=255)
                            gCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCa                                                                                                                          <  1:575616/150‑1 (MQ=255)
                               acaACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAgg                                                                                                                      >  3:117083/1‑151 (MQ=255)
                                catcGATCTGGCCAATCTCGGCTTTCTCATTGCCCGCCAGCTCAATTGCGCGTGTCGCCGCTTCATAGGCCATGGTTGATACGGTTTCGTTTGGCGCGTCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCagag                                                                                                                       <  6:231602/146‑1 (MQ=255)
                                   cGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTc                                                                                                                  >  2:143895/1‑151 (MQ=255)
                                       cAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTccacc                                                                                                              <  6:668226/151‑1 (MQ=255)
                                        aGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTccacca                                                                                                             <  8:1302758/151‑1 (MQ=255)
                                         ggCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCAt                                                                                                            <  8:352656/151‑1 (MQ=255)
                                             aaTCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCAttttt                                                                                                        <  5:1114460/151‑1 (MQ=255)
                                                cTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTcc                                                                                                     >  6:114238/1‑151 (MQ=255)
                                                 tGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTcc                                                                                                     >  6:955587/1‑150 (MQ=255)
                                                  ggTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTc                                                                                                      <  6:1368525/148‑1 (MQ=255)
                                                  ggTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCaa                                                                                                   <  3:1332579/151‑1 (MQ=255)
                                                  ggTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTGTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTc                                                                                                      <  1:1099818/148‑1 (MQ=255)
                                                  cgTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCAtttttt                                                                                                       <  7:170533/146‑1 (MQ=255)
                                                   gTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGCCGCGGCAATGTGGGGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCaa                                                                                                   >  5:264672/1‑150 (MQ=255)
                                                       ttCTCACTGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATc                                                                                                <  7:10496/149‑1 (MQ=255)
                                                       ttCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATc                                                                                                <  2:957095/149‑1 (MQ=255)
                                                       ttCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATc                                                                                                <  2:1276180/149‑1 (MQ=255)
                                                       ttCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATc                                                                                                <  8:24140/149‑1 (MQ=255)
                                                       ttCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCgg                                                                                              >  5:406950/1‑151 (MQ=255)
                                                       ttCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCg                                                                                               <  1:46243/150‑1 (MQ=255)
                                                           cAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGc                                                                                             <  4:1168868/148‑1 (MQ=255)
                                                           cAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGtt                                                                                          >  4:607853/1‑151 (MQ=255)
                                                            aaTGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGttt                                                                                         <  4:1111519/151‑1 (MQ=255)
                                                            aaTGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGATTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGttt                                                                                         >  6:525036/1‑151 (MQ=255)
                                                              tGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGt                                                                                       >  5:811367/1‑151 (MQ=255)
                                                               gCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCCCTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTc                                                                                      >  1:70272/1‑151 (MQ=255)
                                                               gCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTc                                                                                      >  8:17601/1‑151 (MQ=255)
                                                                 ccGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCg                                                                                    <  6:1485596/151‑1 (MQ=255)
                                                                    ccATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCAc                                                                                 >  1:282506/1‑151 (MQ=255)
                                                                    ccATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCCTTTGTCCGCAc                                                                                 <  1:1479945/151‑1 (MQ=255)
                                                                     cATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACt                                                                                <  5:1022985/151‑1 (MQ=255)
                                                                      aTCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACtt                                                                               >  8:1405833/1‑151 (MQ=255)
                                                                          cAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTg                                                                              <  1:641378/148‑1 (MQ=255)
                                                                          cAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGt                                                                             <  3:650135/149‑1 (MQ=255)
                                                                           aaTTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCg                                                                          >  5:922599/1‑151 (MQ=255)
                                                                           aaTTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCg                                                                          >  1:1048451/1‑151 (MQ=255)
                                                                            aTTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCgg                                                                         <  5:1174902/151‑1 (MQ=255)
                                                                              tGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGc                                                                       >  5:614316/1‑151 (MQ=255)
                                                                               gcgcGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGc                                                                       >  1:196630/1‑150 (MQ=255)
                                                                                cgcgTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCAGCACTTGTTCGGGc                                                                       >  4:492866/1‑149 (MQ=255)
                                                                                  cgTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGAt                                                                   <  5:848493/151‑1 (MQ=255)
                                                                                   gtgtCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATa                                                                  <  5:1557288/151‑1 (MQ=255)
                                                                                   gtgtCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATa                                                                  <  3:935673/151‑1 (MQ=255)
                                                                                     gtCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGc                                                                <  6:407912/151‑1 (MQ=255)
                                                                                     ggcgccgcTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGGCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGc                                                                <  8:641588/149‑1 (MQ=255)
                                                                                      tCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCt                                                               >  3:286737/1‑151 (MQ=255)
                                                                                        gccgcTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGc                                                             >  5:327831/1‑151 (MQ=255)
                                                                                        gccgcTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGc                                                             >  7:728047/1‑151 (MQ=255)
                                                                                          cgcTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGcc                                                            >  2:1528515/1‑150 (MQ=255)
                                                                                          cgcTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGATCGCGGAGCCCGGTACGAGTGCCAATCGACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGcc                                                            >  2:405142/1‑150 (MQ=255)
                                                                                           gcTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAg                                                          <  1:1241884/151‑1 (MQ=255)
                                                                                             ttCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGt                                                         >  7:833900/1‑150 (MQ=255)
                                                                                               cAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTAc                                                       <  1:481920/150‑1 (MQ=255)
                                                                                                 aaGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCaa                                                    <  4:1298851/151‑1 (MQ=255)
                                                                                                 aaGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCa                                                     >  2:715704/1‑150 (MQ=255)
                                                                                                    cccATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCaata                                                  >  7:211553/1‑150 (MQ=255)
                                                                                                     ccATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCaataat                                                <  7:347723/151‑1 (MQ=255)
                                                                                                       aTGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCt                                              <  3:1450171/151‑1 (MQ=255)
                                                                                                          gTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCg                                           <  3:1501640/151‑1 (MQ=255)
                                                                                                               aaCGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATAc                                      <  1:395195/151‑1 (MQ=255)
                                                                                                                aCGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACa                                     <  5:19398/151‑1 (MQ=255)
                                                                                                                 cGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACAt                                    <  5:1218770/151‑1 (MQ=255)
                                                                                                                  ggTTTCGTTTGGCGCGGCAATGTGGCGTTCGCTGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATg                                   <  2:880804/151‑1 (MQ=255)
                                                                                                                  ggTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATGCACTCGTCAGAGGTGTCCTCCATTTTTTCCAAATCGGAGTTTCTCCGCACTTGTTCGGGCAGATAGCTGCAAGTACCAATAATCTTCGTATACAtt                                   <  2:609186/151‑2 (MQ=255)
                                                                                                                  ggTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATg                                   >  7:1502638/1‑151 (MQ=255)
                                                                                                                     ttCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTa                                 <  4:770083/150‑1 (MQ=255)
                                                                                                                      tCGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTAc                                <  4:244309/150‑1 (MQ=255)
                                                                                                                       cggttGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGATTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGc                              <  4:1352703/148‑1 (MQ=255)
                                                                                                                       cGTTTGGCGCGGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACg                               <  7:1351876/150‑1 (MQ=255)
                                                                                                                               gcgGCAATGTGGCGTTCGCGGATACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCAc                      >  3:451089/1‑151 (MQ=255)
                                                                                                                               gcgGCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCAc                      >  5:739353/1‑151 (MQ=255)
                                                                                                                                  gCAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACttt                   <  5:997430/151‑1 (MQ=255)
                                                                                                                                   cAATGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACtttt                  <  3:1076044/151‑1 (MQ=255)
                                                                                                                                    aaTGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTc                 <  1:798186/151‑1 (MQ=255)
                                                                                                                                    aaTGTGGCGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTc                 <  2:32021/151‑1 (MQ=255)
                                                                                                                                           cGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTtat           >  6:303946/1‑150 (MQ=255)
                                                                                                                                           cGTTCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTtat           >  2:1119391/1‑150 (MQ=255)
                                                                                                                                              tCGCGGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTtatata        <  4:3287/150‑1 (MQ=255)
                                                                                                                                               cgcgGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATAc       <  8:284482/150‑1 (MQ=255)
                                                                                                                                                 cgGAGACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATACCGt    >  2:466883/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGGGAAAGCGTGCGTAGCAGAAGTCGTTGCCACAACGATCAGGCCAATCTGGTCTTTCTCAATGCCCGCCATCTCAATTGCGCGTGTCGCCGCTTCAAAGCCCATGGTTGAAACGGTTTCGTTTGGCGCGGCAATGTGGCGTTCGCGGATACCGGTACGAGTGACAATCCACTCGTCAGAGGTGTCCACCATTTTTTCCAAATCGGCGTTTGTCCGCACTTGTTCGGGCAGATAGCTGCCAGTACCAATAATCTTCGTATACATGTACGCTCAGTCACTTTTCGGTTATATACCGTCA  >  DH1/2730717‑2731014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑