Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 4,137,737 G→A intergenic (‑31/+145) sugE ← / ← ecnB quaternary ammonium compound‑resistance protein SugE/entericidin B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,137,7370GA98.7% 1701.2 / 4.8 443intergenic (‑31/+145)sugE/ecnBquaternary ammonium compound‑resistance protein SugE/entericidin B
Reads supporting (aligned to +/- strand):  ref base G (1/5);  new base A (241/196);  total (242/201)
Fisher's exact test for biased strand distribution p-value = 9.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 444 total aligned reads displayed.

ATCATCGCCGTCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGTTATTGCTTA  >  DH1/4137588‑4137884
                                                                                                                                                     |                                                                                                                                                   
  catcGCCGTCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTg                                                                                                                                                  <  7:658386/151‑1 (MQ=255)
   atcGCCGTCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGa                                                                                                                                                 <  1:1280803/151‑1 (MQ=255)
      gCCGTCACAGTAATAACACTCGGCGTCAAACGACTAAAGCAGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGaaaa                                                                                                                                              <  7:1308352/151‑1 (MQ=255)
          tCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAgg                                                                                                                                          >  1:117046/1‑151 (MQ=255)
           cacaGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGc                                                                                                                                         <  7:767138/151‑1 (MQ=255)
            acaGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCg                                                                                                                                        >  6:151847/1‑151 (MQ=255)
                       acTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGgtcg                                                                                                                             >  7:1030247/1‑151 (MQ=255)
                        cTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGgtcgt                                                                                                                            >  2:53674/1‑151 (MQ=255)
                          cGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGgtcgt                                                                                                                            <  4:1129620/149‑1 (MQ=255)
                            gCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTg                                                                                                                        >  1:36216/1‑151 (MQ=255)
                                cAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCaa                                                                                                                     >  2:1487437/1‑150 (MQ=255)
                                cAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGCCGTTTGCAGGGCGTCCTGCaa                                                                                                                     >  6:104208/1‑150 (MQ=255)
                                 aaaCGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCaa                                                                                                                     >  5:925621/1‑149 (MQ=255)
                                  aaCGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGc                                                                                                                  >  8:651173/1‑151 (MQ=255)
                                   aCGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCt                                                                                                                 <  4:864677/151‑1 (MQ=255)
                                    cGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGcat                                                                                                                >  8:1542053/1‑149 (MQ=255)
                                    cGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTg                                                                                                                <  1:155076/151‑1 (MQ=255)
                                     gACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTg                                                                                                                >  5:1277668/1‑150 (MQ=255)
                                      aCTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGAt                                                                                                              <  5:1552690/151‑1 (MQ=255)
                                      aCTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGAt                                                                                                              >  7:880249/1‑151 (MQ=255)
                                      aCTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGAt                                                                                                              >  4:439588/1‑151 (MQ=255)
                                      aCTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCACGTCGTCCTGCAAAGCTGAt                                                                                                              >  1:244838/1‑151 (MQ=255)
                                       cTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATg                                                                                                             >  3:1433697/1‑151 (MQ=255)
                                           aGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTg                                                                                                         >  2:1376590/1‑151 (MQ=255)
                                             ccGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGa                                                                                                        >  8:320304/1‑150 (MQ=255)
                                              cGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAAc                                                                                                      <  4:669418/151‑1 (MQ=255)
                                              cGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAAc                                                                                                      <  7:1105446/151‑1 (MQ=255)
                                                  ggTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGat                                                                                                  >  6:1363964/1‑151 (MQ=255)
                                                  ggTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGGCCTGCAAACCTGATGGTTGAACGCat                                                                                                  >  1:949028/1‑151 (MQ=255)
                                                   gTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGat                                                                                                  >  2:307987/1‑150 (MQ=255)
                                                       atTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATAttt                                                                                              <  4:1248123/150‑1 (MQ=255)
                                                         ttCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTg                                                                                            <  2:61737/150‑1 (MQ=255)
                                                         ttCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTg                                                                                            <  6:925267/150‑1 (MQ=255)
                                                         ttCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTg                                                                                            <  3:899077/150‑1 (MQ=255)
                                                         ttCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGc                                                                                           >  2:667183/1‑151 (MQ=255)
                                                         ttCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGc                                                                                           >  8:1324584/1‑151 (MQ=255)
                                                           cAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGcc                                                                                          >  2:913967/1‑150 (MQ=255)
                                                           cAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCa                                                                                         <  3:184069/151‑1 (MQ=255)
                                                           cAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGCACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAACAGGCGTTTGCAGGTCGTCCTGCACAGCTGATGGTTGAAGGGATATTTTGcc                                                                                          >  4:1460232/1‑150 (MQ=255)
                                                            aGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGcc                                                                                          >  1:680587/1‑149 (MQ=255)
                                                               ccAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTg                                                                                     <  1:581767/151‑1 (MQ=255)
                                                                cAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTgg                                                                                    <  1:492566/151‑1 (MQ=255)
                                                                  aCGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTAGCCAGGTGGCa                                                                                  <  8:1157291/151‑1 (MQ=255)
                                                                        cATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGa                                                                             >  8:403450/1‑150 (MQ=255)
                                                                        cATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGa                                                                             >  3:1347424/1‑150 (MQ=255)
                                                                        cATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGa                                                                             >  4:1029570/1‑150 (MQ=255)
                                                                         aTACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAAt                                                                           >  8:771762/1‑151 (MQ=255)
                                                                             cACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCa                                                                       <  6:599482/151‑1 (MQ=255)
                                                                                 tCCAGAAGACCAGCACTAACTAAGATAATCGAGGACATATCAGGCTCCGGAGAATTGGGGCAGTCGAAAGTGAAAAGAGGCGTTTGCATGGCGTCCTGAAAAGCTGATGGTTGAACTGATATTTTGCCAGTTGTCAGGGGAATTTCAACt                                                                    <  5:214606/150‑1 (MQ=255)
                                                                                  ccAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTAt                                                                  >  8:19728/1‑151 (MQ=255)
                                                                                   cagcagACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTAtt                                                                 >  5:690436/1‑151 (MQ=255)
                                                                                   cagcagACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTAtt                                                                 >  3:29359/1‑151 (MQ=255)
                                                                                    agcagACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTa                                                                <  6:168179/151‑1 (MQ=255)
                                                                                    agcagACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTa                                                                <  3:1102723/151‑1 (MQ=255)
                                                                                     gcagACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTAc                                                               <  5:869316/151‑1 (MQ=255)
                                                                                     gcagACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTAc                                                               <  7:1348371/151‑1 (MQ=255)
                                                                                      cagACCAGCAATAACTAAGATAATCAAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAAAGGATATTTTGACAGGTGGCAGGGGAATTTCAACTATTAcc                                                              >  3:1376666/1‑151 (MQ=255)
                                                                                        gACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCtt                                                            <  5:936241/151‑1 (MQ=255)
                                                                                         aCCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCttt                                                           <  6:393375/151‑1 (MQ=255)
                                                                                         aCCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCttt                                                           >  6:1179348/1‑151 (MQ=255)
                                                                                         aCCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCttt                                                           <  4:958063/151‑1 (MQ=255)
                                                                                          ccAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCtttt                                                          >  8:560068/1‑150 (MQ=255)
                                                                                          ccAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTtg                                                          <  2:1340188/151‑1 (MQ=255)
                                                                                          ccAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTtg                                                          <  3:1524596/151‑1 (MQ=255)
                                                                                           cAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTtgt                                                         >  5:1036818/1‑151 (MQ=255)
                                                                                               aaTAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACt                                                     <  5:416736/151‑1 (MQ=255)
                                                                                                aTAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGGTGCTGGTTGCACGGATATTTTTCCAGGTGGGAGGGGAATTTAAACTATTACCTTTTTGCCTAt                                                   >  3:634027/1‑151 (MQ=255)
                                                                                                 tAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTAt                                                   >  7:262518/1‑151 (MQ=255)
                                                                                                  aaCTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATc                                                  >  5:354254/1‑151 (MQ=255)
                                                                                                    cTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGATTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGc                                                >  3:387327/1‑151 (MQ=255)
                                                                                                      aaGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGc                                                <  2:768286/149‑1 (MQ=255)
                                                                                                      aaGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGc                                                <  7:159668/149‑1 (MQ=255)
                                                                                                         aTAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGaa                                            >  5:263002/1‑150 (MQ=255)
                                                                                                            aTCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGaaaaa                                         >  2:1269228/1‑150 (MQ=255)
                                                                                                            aTCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGaaaaa                                         >  7:407903/1‑150 (MQ=255)
                                                                                                            aTCCAGGACATATCAGGCTCCGGAGAATTGCGGCCGTCCCCGCTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGCTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGaaaaaa                                        <  6:772095/151‑1 (MQ=255)
                                                                                                             tCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAaaac                                       >  5:744290/1‑151 (MQ=255)
                                                                                                               cAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAaaaca                                      >  1:817403/1‑150 (MQ=255)
                                                                                                                aGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAaaacaa                                     >  4:614051/1‑150 (MQ=255)
                                                                                                                aGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAaaacaa                                     >  4:1422104/1‑150 (MQ=255)
                                                                                                                  gACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACg                                  <  5:1510505/151‑1 (MQ=255)
                                                                                                                     atatCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGc                                >  3:1237563/1‑150 (MQ=255)
                                                                                                                     atatCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGc                                >  2:112196/1‑150 (MQ=255)
                                                                                                                       atCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACg                             <  2:996380/151‑1 (MQ=255)
                                                                                                                       atCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACg                             >  1:1000718/1‑151 (MQ=255)
                                                                                                                        tCAGGCTCCGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACg                             >  4:274869/1‑150 (MQ=255)
                                                                                                                                cGGAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTg                    <  4:1540579/151‑1 (MQ=255)
                                                                                                                                 ggAGAATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGCCACAGCTGt                   >  5:578423/1‑151 (MQ=255)
                                                                                                                                  gagaATTGGGGCCGTCCCCAGTTAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTc                  <  3:479349/151‑1 (MQ=255)
                                                                                                                                  gagaATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTc                  <  2:43342/151‑1 (MQ=255)
                                                                                                                                   agaATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCg                 >  8:1012020/1‑151 (MQ=255)
                                                                                                                                    gaATTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTAGCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTGGt                >  5:1384106/1‑151 (MQ=255)
                                                                                                                                     aaTTGGGGCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTa               <  2:1229661/151‑1 (MQ=255)
                                                                                                                                         ggggCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGt           <  7:554707/151‑1 (MQ=255)
                                                                                                                                         ggggCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGt           >  3:1466488/1‑151 (MQ=255)
                                                                                                                                            gCCGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGttat        >  4:775478/1‑151 (MQ=255)
                                                                                                                                             ccGTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGttatt       >  2:194781/1‑151 (MQ=255)
                                                                                                                                               gTCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGttattgc     <  5:1175570/151‑1 (MQ=255)
                                                                                                                                                tCCCCAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGttattgct    >  3:799023/1‑151 (MQ=255)
                                                                                                                                                 ccccAGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGttattgctt   >  8:884063/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATCATCGCCGTCACAGTAATAACACTCGGCGTCAAACGACTAAAGCCGTGGGTATATTTCAGGCCAACGGCCCATACCACTTCCAGCAGACCAGCAATAACTAAGATAATCCAGGACATATCAGGCTCCGGAGAATTGGGGCCGTCCCCGGTGAAAAGAGGCGTTTGCAGGTCGTCCTGCAAAGCTGATGGTTGAACGGATATTTTGCCAGGTGGCAGGGGAATTTCAACTATTACCTTTGTGACTATCGCAGAAAAAACAAACGGCACGACACAGCTGTCGTACCGTTATTGCTTA  >  DH1/4137588‑4137884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: