Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 4,547,872 C→T G61E (GGA→GAA)  rho ← transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,547,8720CT98.2% 1234.4 / 5.8 328G61E (GGA→GAA) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base C (2/4);  new base T (147/174);  total (149/179)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

Only 100 of 336 total aligned reads displayed.

ATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTCGCCGAGAGTGATCAGCTCAG  >  DH1/4547724‑4548019
                                                                                                                                                    |                                                                                                                                                   
 tAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGTAGCTGTCTGCGGAACGGAGGAAACCAAattca                                                                                                                                                   >  5:673297/1‑150 (MQ=255)
 tAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAattcat                                                                                                                                                  >  2:403935/1‑151 (MQ=255)
  aGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAattcatc                                                                                                                                                 <  7:719880/151‑1 (MQ=255)
  aGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatccat                                                                                                                                                  <  5:395292/150‑1 (MQ=255)
       tCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGc                                                                                                                                             >  2:1136270/1‑150 (MQ=255)
        cACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCaa                                                                                                                                           <  2:886914/151‑1 (MQ=255)
        cACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCa                                                                                                                                            <  6:552838/150‑1 (MQ=255)
         aCCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATGACCAGTGCGGAGGTTGAAACGGCGGATTTCGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTGTGGGCAACGGAGGAAACCAAATTCATCCTGCaa                                                                                                                                           >  4:1123438/1‑150 (MQ=255)
         aCCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAat                                                                                                                                          >  3:433379/1‑151 (MQ=255)
         aCCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAat                                                                                                                                          >  1:596837/1‑151 (MQ=255)
          ccTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGACACCAAATTCATCCTGCAat                                                                                                                                          >  7:1502748/1‑150 (MQ=255)
             tcttTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATAtct                                                                                                                                      >  4:662719/1‑151 (MQ=255)
                    gcgggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTAc                                                                                                                               <  5:1311142/151‑1 (MQ=255)
                    gcgggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTAc                                                                                                                               >  1:1517784/1‑151 (MQ=255)
                     ggggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACg                                                                                                                              <  3:621189/150‑1 (MQ=255)
                     agggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGCCCGGCGAGGTAGGAGCTGTCTGCGGAACTGAGGAAACCAAATTCATCCTGCAATATATCCAGTACg                                                                                                                              >  8:7398/2‑151 (MQ=255)
                         cgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAACCCAAATTCATCCTGCAATATCTCCAGTACGCCAt                                                                                                                          >  3:1531607/1‑151 (MQ=255)
                         cgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCAt                                                                                                                          >  7:629676/1‑151 (MQ=255)
                         cgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCAt                                                                                                                          >  8:1266509/1‑151 (MQ=255)
                         cgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCAt                                                                                                                          >  7:1217808/1‑151 (MQ=255)
                             tCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCAcc                                                                                                                      >  8:1193636/1‑151 (MQ=255)
                                taacAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCAcc                                                                                                                      <  1:714291/145‑1 (MQ=255)
                                 aCCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCaa                                                                                                                    >  7:137741/1‑149 (MQ=255)
                                 aCCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCaa                                                                                                                    >  1:1010431/1‑149 (MQ=255)
                                  ccAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCaa                                                                                                                    >  6:589034/1‑148 (MQ=255)
                                   cAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCaa                                                                                                                    >  7:443926/1‑147 (MQ=255)
                                   cAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGa                                                                                                                 >  6:1182730/1‑150 (MQ=255)
                                   cAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATATGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCAACCTGAAATATCTCCAGTACGCCATCACCaa                                                                                                                    >  2:544294/1‑147 (MQ=255)
                                      agaTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATc                                                                                                             >  6:859197/1‑151 (MQ=255)
                                      agaTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATc                                                                                                             >  8:334052/1‑151 (MQ=255)
                                          ggTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCg                                                                                                         >  4:1483409/1‑151 (MQ=255)
                                           gTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTctc                                                                                                        >  6:1544537/1‑149 (MQ=255)
                                           gTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGc                                                                                                        <  5:230229/151‑1 (MQ=255)
                                            tATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGcc                                                                                                       >  1:1093216/1‑151 (MQ=255)
                                              tCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCAc                                                                                                     >  4:528615/1‑151 (MQ=255)
                                                cccAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCActc                                                                                                   <  5:852394/150‑1 (MQ=255)
                                                aCCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCActc                                                                                                   <  3:1000992/151‑1 (MQ=255)
                                                 ccAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCActct                                                                                                  <  5:1489459/151‑1 (MQ=255)
                                                  cAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCtt                                                                                                 >  7:192462/1‑151 (MQ=255)
                                                    gTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTg                                                                                               >  6:305711/1‑151 (MQ=255)
                                                      gCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGc                                                                                              <  7:1046371/150‑1 (MQ=255)
                                                      gCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCg                                                                                             >  6:716067/1‑151 (MQ=255)
                                                      gCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCg                                                                                             >  4:463281/1‑151 (MQ=255)
                                                        ggAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtg                                                                                           >  6:349582/1‑151 (MQ=255)
                                                         gAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtgc                                                                                          <  8:927683/151‑1 (MQ=255)
                                                         gAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTACGCCACTCTTTGCGtgc                                                                                          >  2:12198/1‑151 (MQ=255)
                                                          aGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtgct                                                                                         >  7:960417/1‑151 (MQ=255)
                                                              tGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTgctgct                                                                                      <  8:399343/150‑1 (MQ=255)
                                                               gAAAGGGCCGATTTGGCTAGCGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTCCGCAACGCAGGAAACCAAATACATCCTCCAATATCTCCACTACGCCATCCCCAAAGATATCTTCGCCACTCTTTGCGTGCTGCtt                                                                                     <  4:1280453/150‑1 (MQ=255)
                                                               gAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTc                                                                                    <  4:130897/151‑1 (MQ=255)
                                                                aaaCGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTc                                                                                    >  6:1448271/1‑150 (MQ=255)
                                                                  aCGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCATGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAgg                                                                                 >  4:27484/1‑151 (MQ=255)
                                                                   cggcggATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAgg                                                                                 >  8:1542601/1‑150 (MQ=255)
                                                                    ggcggATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGAt                                                                               <  4:946357/151‑1 (MQ=255)
                                                                      cggATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCCCCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATgg                                                                             <  5:1397107/151‑1 (MQ=255)
                                                                       ggATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGc                                                                            <  5:1063085/151‑1 (MQ=255)
                                                                       ggATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTCCGTGCTGCTTCAGGATGGc                                                                            >  6:913521/1‑151 (MQ=255)
                                                                         aTTTGGCTAGGGGAAACGCACATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaa                                                                          <  1:931900/151‑1 (MQ=255)
                                                                           ttGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaaa                                                                         >  1:1136064/1‑150 (MQ=255)
                                                                           ttGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaaa                                                                         <  3:1159856/150‑1 (MQ=255)
                                                                           ttGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaaa                                                                         >  2:803114/1‑150 (MQ=255)
                                                                           ttGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaaa                                                                         >  5:1232670/1‑150 (MQ=255)
                                                                             ggCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaat                                                                      <  3:1325703/151‑1 (MQ=255)
                                                                              gCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaata                                                                     <  6:1334940/151‑1 (MQ=255)
                                                                                tAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaataat                                                                   >  8:498810/1‑151 (MQ=255)
                                                                                 tggggAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaataat                                                                   <  3:905626/149‑1 (MQ=255)
                                                                                  ggggAAACGTAGATGTCATCTGGACCGGCGAGGTTGGAGCCGTCTGCGGAACGGACGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGt                                                                 <  4:1391838/151‑1 (MQ=255)
                                                                                    ggAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTcc                                                               <  7:847979/151‑1 (MQ=255)
                                                                                          gTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTa                                                         <  3:373800/151‑1 (MQ=255)
                                                                                            aGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACg                                                       >  6:1511811/1‑151 (MQ=255)
                                                                                              aTGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAAATCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCa                                                     <  6:1279177/151‑1 (MQ=255)
                                                                                                gTCATGAGGACCGGCGAGGTAGGAGCTGGCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGTTATCTTCGCCACTCTTTGCGTGCTGCTTCCGGATGGCAAAAATAATGTCCTGCTTACGCATa                                                   <  4:1440738/151‑1 (MQ=255)
                                                                                                gTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCAt                                                    <  3:479945/150‑1 (MQ=255)
                                                                                                 tcatcaGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATAc                                                  >  5:1136815/1‑151 (MQ=255)
                                                                                                   atcaGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGa                                                <  6:661955/151‑1 (MQ=255)
                                                                                                         aCCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAgg                                          <  4:1071445/151‑1 (MQ=255)
                                                                                                         aCCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCACTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTTCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAgg                                          <  5:600467/151‑1 (MQ=255)
                                                                                                           cGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGtt                                        >  7:330323/1‑151 (MQ=255)
                                                                                                           cGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGtt                                        >  4:574861/1‑151 (MQ=255)
                                                                                                            ggCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGttt                                       <  2:1551672/151‑1 (MQ=255)
                                                                                                             gCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCCGCTTACGCATACGAGCCAGGtttt                                      <  5:88229/151‑1 (MQ=255)
                                                                                                               gAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTcc                                    >  1:1346974/1‑151 (MQ=255)
                                                                                                               gAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTcc                                    >  2:621523/1‑151 (MQ=255)
                                                                                                                aGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTcc                                    >  3:769479/1‑150 (MQ=255)
                                                                                                                   tAGGAGCTGTCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGcc                                >  7:755446/1‑151 (MQ=255)
                                                                                                                            tCTGCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATAtttt                       >  4:1004315/1‑151 (MQ=255)
                                                                                                                               gCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAg                                  <  1:834087/137‑1 (MQ=255)
                                                                                                                               gCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATAtttt                       <  5:1178843/148‑1 (MQ=255)
                                                                                                                               gCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATAtttt                       <  4:816989/148‑1 (MQ=255)
                                                                                                                               gCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTc                      <  4:1204347/149‑1 (MQ=255)
                                                                                                                               gCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTCg                     <  2:1385328/150‑1 (MQ=255)
                                                                                                                               gCGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTCg                     <  1:400085/150‑1 (MQ=255)
                                                                                                                                cGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTCGcc                   <  2:347308/151‑1 (MQ=255)
                                                                                                                                cGGAACGGAGGAAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTCGcc                   >  6:498220/1‑151 (MQ=255)
                                                                                                                                      ggaggaAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTCGCCGAGAGt             >  8:1318878/1‑151 (MQ=255)
                                                                                                                                        aggaAACCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTCGCCGAGAGTGa           <  3:1260212/151‑1 (MQ=255)
                                                                                                                                             aCCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTCGCCGAGAGTGATCAGc      <  3:1071417/151‑1 (MQ=255)
                                                                                                                                             aCCAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTCGCCGAGAGTGATCAGc      <  3:953564/151‑1 (MQ=255)
                                                                                                                                               cAAATTCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTCGCCGAGAGTGATCAGCTc    >  5:487747/1‑151 (MQ=255)
                                                                                                                                                aaaTTCATCGTGCAATATCTCCAGTACGCCATCACCAAACTTATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTCGCCGAGAGTGATCAGCTCa   <  4:361936/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATATCTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTTACGCATACGAGCCAGGTTTTCCAGCCCCATATTTTCGCCGAGAGTGATCAGCTCAG  >  DH1/4547724‑4548019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑