| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | DH1 | 3,668,924 | (T)5→6 | pseudogene (353/455 nt) | lpxA ← | hydroxymyristoyl‑ACP dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | DH1 | 3,668,919 | 1 | . | T | 96.6% | 1025.9 / 21.8 | 292 | pseudogene (358/455 nt) | lpxA | hydroxymyristoyl‑ACP dehydratase |
| Reads supporting (aligned to +/- strand): ref base . (8/2); new base T (115/166); total (124/168) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 309 total aligned reads displayed.
CCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑TT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT > DH1/3668770‑3669064 | tCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑tt‑tt < 4:1138653/149‑1 (MQ=255) acaATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑tt‑ttttcg > 1:613842/1‑147 (MQ=255) aTGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAg < 2:187169/151‑1 (MQ=255) ggCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTg < 1:193152/151‑1 (MQ=255) ggCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTg > 6:870628/1‑151 (MQ=255) cGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGAc < 6:1210724/151‑1 (MQ=255) cGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCCGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGACCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGAc > 7:202881/1‑151 (MQ=255) ttGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTc > 3:193563/1‑151 (MQ=255) ggATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCa < 7:842198/151‑1 (MQ=255) gCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCCGCTACGAGCACACATCATCGTTTCTTAGCAAACTACTTTACCATCGACCAGAGCACCCACTTTAAAACGCTTCAGGCCGCGGCGC‑GTTT‑TTTGGAAAGTGAATTCCATGAt > 8:423655/1‑151 (MQ=255) caAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCAt < 8:487830/151‑1 (MQ=255) caAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCAt > 8:1256157/1‑151 (MQ=255) ggCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGa < 8:675302/151‑1 (MQ=255) gCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGAt < 1:1057261/151‑1 (MQ=255) aTTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGcc < 7:421160/151‑1 (MQ=255) atcaatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCGAGGc < 1:279090/151‑1 (MQ=255) atcaatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAgg < 3:95709/150‑1 (MQ=255) atcaatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGc < 5:582697/151‑1 (MQ=255) caatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGG‑TT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCAc > 7:1120836/1‑151 (MQ=255) caatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCAc < 2:1240157/151‑1 (MQ=255) aatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACg < 3:222735/151‑1 (MQ=255) aatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGA‑GTTT‑TTTCGAAATTGACTTCCATGATCATTTGATCGCCAGGCACg > 3:1108242/1‑151 (MQ=255) aatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTCCTTCGCAAACTACTTTACAATCGACCAGAGCAACACCGTTAAAACGGGTCAGGCCGCCGCGC‑GAAA‑TATCCAAAGAGACTTCCATCCTCATTTGATCGCCAGGCAcc > 6:197553/1‑150 (MQ=255) atcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGa < 5:1261904/151‑1 (MQ=255) atcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑TTATTTCGAAAGTGACTTCCATGATCATTTGATCGCcacgcacg > 4:835679/1‑150 (MQ=255) aCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCg < 2:1030994/151‑1 (MQ=255) aCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCg > 3:1158387/1‑151 (MQ=255) cGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCgg < 8:466846/151‑1 (MQ=255) aTCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTGGCAAACTACTTTACCATCGACCAGACCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgc < 5:697066/150‑1 (MQ=255) tCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgcgc > 2:388723/1‑151 (MQ=255) cggCCTCCCGGCTACGAGCCCACATGATCGTGGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGCGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCtt < 6:690960/150‑1 (MQ=255) aGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCtt > 1:855033/1‑151 (MQ=255) ccTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGaa < 8:879329/151‑1 (MQ=255) gcccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgc < 1:1004174/150‑1 (MQ=255) cGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgcg > 2:308744/1‑151 (MQ=255) ggCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgcgc < 6:214224/151‑1 (MQ=255) gCTACGAGCACACATCATCGGTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCt < 7:263569/151‑1 (MQ=255) cTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGAGTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCtt > 4:291640/1‑151 (MQ=255) tACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCATTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTc > 2:993562/1‑151 (MQ=255) gaCGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTc < 6:321147/150‑1 (MQ=255) cGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGt < 3:684823/151‑1 (MQ=255) cGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGt < 6:1261451/151‑1 (MQ=255) ccacacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGGGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCaa < 4:1241720/150‑1 (MQ=255) cacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATAc < 5:636014/151‑1 (MQ=255) acaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCGTTAAAACGGGTCAGGCCGCGGCGCGG‑TT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATAcc < 2:505357/151‑1 (MQ=255) catcatCGTTGCTTCGGAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGGCGGGGCGC‑GTTT‑TTTAGAAAGTGTCTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCa < 4:1045741/151‑1 (MQ=255) catcatCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGG‑TT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATAcc > 5:231014/1‑150 (MQ=255) catcatCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATAcc > 2:476984/1‑150 (MQ=255) atcatcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCCTTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCac > 6:164373/1‑150 (MQ=255) catcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCg > 1:1212946/1‑151 (MQ=255) catcGGTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGc < 8:591863/150‑1 (MQ=255) atcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGa < 3:1215601/151‑1 (MQ=255) tcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCCGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGa > 2:567900/1‑150 (MQ=255) cGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGa > 4:731844/1‑149 (MQ=255) cGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAag < 4:186536/151‑1 (MQ=255) ttGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTGGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACCACCCGGCGCGTGAAGCCCGCGTGGTGAATACCCGCGAagt > 8:1145520/1‑150 (MQ=255) tGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagta < 5:8594/150‑1 (MQ=255) ttCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTAc > 7:1002582/1‑151 (MQ=255) tCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAgtagta < 7:950352/149‑1 (MQ=255) tCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTAc > 5:395982/1‑150 (MQ=255) gCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACCGc > 2:713661/1‑151 (MQ=255) gCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCCAAAGTGCCTTCCATGATCATTTGATGGCCAGGCACGACCGGGCCCTTGAAGCGCGCTTCGTGTATTCCACCGAACTAGTTCAgg > 3:425632/1‑150 (MQ=255) aaaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTc > 2:265085/1‑151 (MQ=255) aaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCa < 4:787276/151‑1 (MQ=255) actactTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTTACTTCCATGATCATTTGATCGCCAGGCACGCCCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCGCAc > 3:350054/1‑151 (MQ=255) ttACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCgg < 2:741905/149‑1 (MQ=255) ttACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCgg < 4:452951/149‑1 (MQ=255) tACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGtt < 6:583116/150‑1 (MQ=255) aCCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTc < 8:30029/150‑1 (MQ=255) aCCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAAGACCAGCGAAGTAGTACAGCTCACCCGGTTcc > 3:923920/1‑151 (MQ=255) ccATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCa < 1:995110/151‑1 (MQ=255) ccATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCGTCGTCAATACCAGCGAAGTAGTACAGCTCACCCCGTTcc > 6:1185049/1‑150 (MQ=255) aaaccaCCAGAGCAACCCCTTTAAAACGCGTCAGGCCGCGGATC‑G‑TTATTTCGAAAGTGACTTCAATCATCATATGCTCGCCAGCCACGACCGGGCGCTAGAAGCGCTATTCCTAAATACCAGCCAACTAGTAAATCACACCAGGTTCCat > 4:759809/6‑150 (MQ=255) cGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGCCCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACCGCTCACCCGGGTCCAGttt > 6:504464/1‑151 (MQ=255) cGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGttt > 5:492206/1‑151 (MQ=255) gACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGtttt > 5:1219936/1‑151 (MQ=255) ccAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTcc < 6:177915/151‑1 (MQ=255) agagCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTa < 7:990667/151‑1 (MQ=255) gagCAACCCCTTTAAAACGGGTCAGGCCGCTGCGC‑GTTT‑TTTCGAAAGTGACTTCCATTATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTAc < 3:773747/151‑1 (MQ=255) aaCCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtt < 1:736831/151‑1 (MQ=255) ccccTTTAAACCGGGTCAGGCCGCGGCGC‑GTTT‑TTTCAAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtttt < 4:581015/151‑1 (MQ=255) cccTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTAGGCTTTTa < 4:693164/151‑1 (MQ=255) ccTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTACAGTTTTCCTACGCTTTTaa < 5:277548/151‑1 (MQ=255) cTTTAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTAGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGATCACCCGGTTCCAGTTTTCCTACGCTTTTaa > 5:1030431/1‑150 (MQ=255) ttAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTaaa < 7:34520/149‑1 (MQ=255) ttAAAACGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAAc < 2:1111486/150‑1 (MQ=255) aaaaCGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGcc < 7:181211/151‑1 (MQ=255) aaaCGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCATGAAGCGCGCTTCGTGAATACCAGCGAAGTAGTACAGGGCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGcc > 6:271431/1‑150 (MQ=255) aaCGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAg < 7:692342/151‑1 (MQ=255) aCGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGa < 7:74895/151‑1 (MQ=255) aCGGGTCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCCCTTGAAGCGCGCTTCGTCAATACCAGAGAAGTAGTACAGCTCACCCCGTTCCAGTTTTCCTACGCTTTTAAACGcc > 6:1225132/1‑148 (MQ=255) tCAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGCAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGGTCCAGGTTTCCTACGCTTTTAAACGCCAGCATAcc > 2:860806/1‑151 (MQ=255) cAGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCt < 4:328894/151‑1 (MQ=255) aGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTg < 2:1051617/151‑1 (MQ=255) aGGCCGCGGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTg < 3:328837/151‑1 (MQ=255) cgcgGCGC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGCCCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAAAGCCCGAAAACCTGTTGc > 8:78738/1‑151 (MQ=255) cggcgC‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCt < 6:406367/151‑1 (MQ=255) gcgc‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCtgt < 8:207543/151‑1 (MQ=255) gcgc‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCtgt > 5:935595/1‑151 (MQ=255) gcgc‑GTTT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCtgt < 3:52996/151‑1 (MQ=255) | CCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑TT‑TTTCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT > DH1/3668770‑3669064 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |