BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	DH1	2,201,912	Δ3,035 bp		[pntA]–tqsA	[pntA], pntB, tqsA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	DH1	2201912	2204946	3035	332 [8]	[5] 329	[pntA]–tqsA	[pntA], pntB, tqsA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	DH1	= 2201911	8 (0.020)	321 (0.980)	190/300	0.1	98.0%	coding (1337/1533 nt)	pntA	NAD(P) transhydrogenase subunit alpha
*	?	DH1	2204947 =	5 (0.020)	321 (0.980)	190/300	0.1	98.0%	intergenic (‑382/‑30)	tqsA/mdtJ	AI‑2 transporter TqsA/spermidine export protein MdtJ

Only 100 of 329 total aligned reads displayed.

ACATTTACCCATCTTCGTACCTCATCCACTATCTTGCTCGTAGGTGAGCAGCACTGGCGCAACGCGCTTTAGTCCTTTCTTCTGCCAAAGCTTGCGCTATGCGCGGGGCTTGACCACTTTACTTTGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGGCTTTATTTTAATGCTGGTGATG  >  DH1/2204799‑2205096

gctcaccgtggcgtaaatacgcgttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatctt                                                                                                                                                      >  1:101137‑M2/149‑150 (MQ=255)

gctcaccgtggcgtaaatacgcgttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTa                                                                                                                                                     <  4:549072‑M2/3‑1 (MQ=255)

 ctcaccgtggcgtaaatacgcgttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTa                                                                                                                                                     >  6:264356‑M2/148‑150 (MQ=255)

    accgtggcgtaaatacgcgttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAaat                                                                                                                                                 <  3:1213279‑M2/7‑1 (MQ=255)

     tACCCATCTTCGTACCTCATCCACTATCTTGCTCGTAGGTGAGCAGCACTGGCGCAACGCGCTTTAGTCCTTTCTTCTGCCAAAGCTTGCGCTATGCGCGGGGCTTGACCACTTTACTTTGGTTTCGCTGAATTAAGCGAAAATTAAaat                                                                                                                                                 >  3:1166756/1‑150 (MQ=255)

      cgtggcgtaaatacgcgttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAaataa                                                                                                                                               <  8:509030‑M2/9‑1 (MQ=255)

      cgtggcgtaaatacgcgtggatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAaataa                                                                                                                                               <  5:241597‑M2/9‑1 (MQ=255)

       gtggcgtaaatacgcgttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAaataat                                                                                                                                              <  3:827519‑M2/10‑1 (MQ=255)

         ggcgtaaatacgcgttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATtc                                                                                                                                            <  7:1060532‑M2/12‑1 (MQ=255)

          gcgtaaatacgcgttgatggcgctggtaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATtct                                                                                                                                           <  2:709886‑M2/13‑1 (MQ=255)

           cgtaaatacgcgttgatggcggtggcaatcattctttttggctggatggcaagcggtgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATtctc                                                                                                                                          <  4:279343‑M2/14‑1 (MQ=255)

           cgtaaatacgcgttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATtctc                                                                                                                                          >  7:2575‑M2/138‑151 (MQ=255)

           cgtaaatacgcgttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATtctc                                                                                                                                          <  4:148222‑M2/14‑1 (MQ=255)

            gtaaatacgcgttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTctct                                                                                                                                         >  6:708549‑M2/137‑151 (MQ=255)

              aaatacgcgttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTg                                                                                                                                       >  2:1164818‑M2/135‑151 (MQ=255)

               aatacgcgtggatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGc                                                                                                                                      <  7:901015‑M2/18‑1 (MQ=255)

                atacgcgttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGc                                                                                                                                      >  8:245361‑M2/133‑150 (MQ=255)

                      gttgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGgaga                                                                                                                                >  6:460269‑M2/127‑150 (MQ=255)

                        tgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAg                                                                                                                              <  5:800130‑M2/26‑1 (MQ=255)

                        tgatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAg                                                                                                                              <  7:474206‑M2/26‑1 (MQ=255)

                         gatggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAgg                                                                                                                             <  5:96884‑M2/27‑1 (MQ=255)

                            ggcgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACa                                                                                                                          <  7:390677‑M2/30‑1 (MQ=255)

                              cgctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATg                                                                                                                       <  5:1020191‑M2/33‑1 (MQ=255)

                               gctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttggcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGt                                                                                                                      <  8:1182717‑M2/34‑1 (MQ=255)

                                ctggcaatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGTGAAGGACAATGt                                                                                                                      >  2:1069654‑M2/117‑150 (MQ=255)

                                     aatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTAtattt                                                                                                                <  3:683273‑M2/40‑1 (MQ=255)

                                     aatcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTAtattt                                                                                                                <  7:333152‑M2/40‑1 (MQ=255)

                                      atcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTAtattta                                                                                                               <  1:292722‑M2/41‑1 (MQ=255)

                                       tcattctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTAtatttat                                                                                                              >  4:1000951‑M2/110‑151 (MQ=255)

                                        gattctttttggctggatggcaagcgttgctccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggggtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTAtatttat                                                                                                              <  8:729334‑M2/42‑1 (MQ=255)

                                         attctttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTg                                                                                                            >  7:1128086‑M2/108‑151 (MQ=255)

                                              ttttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGAt                                                                                                         <  8:133216‑M2/47‑1 (MQ=255)

                                               tttggctggatggcaagcgttgcgccgaaagaattccttgggcacttctccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGAttt                                                                                                       <  1:896351‑M2/49‑1 (MQ=255)

                                                ttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGAtttt                                                                                                      <  6:600346‑M2/50‑1 (MQ=255)

                                                ttggctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGAtttt                                                                                                      <  7:224455‑M2/50‑1 (MQ=255)

                                                   gctggatggcaatagttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTttatta                                                                                                  <  4:751334‑M2/54‑1 (MQ=255)

                                                   gctggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTttatta                                                                                                  <  4:427055‑M2/54‑1 (MQ=255)

                                                      ggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGt                                                                                               <  8:835553‑M2/57‑1 (MQ=255)

                                                      ggatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGt                                                                                               <  2:252986‑M2/57‑1 (MQ=255)

                                                       gatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTc                                                                                              <  8:1234010‑M2/58‑1 (MQ=255)

                                                       gatggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTc                                                                                              >  3:515934‑M2/94‑151 (MQ=255)

                                                        atgtcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGTGAAGGACAATGTATATTTATTGGATTTTATTAGGTCt                                                                                             <  7:843829‑M2/59‑1 (MQ=255)

                                                         tggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTg                                                                                            >  7:309051‑M2/92‑151 (MQ=255)

                                                          ggcaagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTg                                                                                            <  3:1049109‑M2/60‑1 (MQ=255)

                                                           gcaagcggtgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTT‑CAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCt                                                                                         <  5:1193053‑M2/62‑1 (MQ=255)

                                                             aagcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTa                                                                                        <  7:797686‑M2/64‑1 (MQ=255)

                                                               gcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTAtt                                                                                      >  8:335493‑M2/86‑151 (MQ=255)

                                                               gcgttgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTAtt                                                                                      <  7:116577‑M2/66‑1 (MQ=255)

                                                                cgtcgcgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTg                                                                                     <  3:189666‑M2/67‑1 (MQ=255)

                                                                     cgccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTAc                                                                                 >  6:292444‑M2/80‑150 (MQ=255)

                                                                       ccgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTAc                                                                                 >  8:413451‑M2/78‑148 (MQ=255)

                                                                        cgaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGaa                                                                             <  2:657632‑M2/75‑1 (MQ=255)

                                                                         gaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGaa                                                                             >  5:468445‑M2/76‑150 (MQ=255)

                                                                         gaaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGaa                                                                             >  1:1259995‑M2/76‑150 (MQ=255)

                                                                          aaagaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAAt                                                                           >  3:973682‑M2/75‑151 (MQ=255)

                                                                            agaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAAtt                                                                          >  6:1009472‑M2/73‑150 (MQ=255)

                                                                             gaattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTAc                                                                        <  6:230060‑M2/80‑1 (MQ=255)

                                                                              aattccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTAcc                                                                       >  4:346325‑M2/71‑151 (MQ=255)

                                                                               attccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTGTTAGGTCTGGCTATTGCTACAGAAATTACCg                                                                      >  1:992076‑M2/70‑151 (MQ=255)

                                                                                ttccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCgg                                                                     >  6:807595‑M2/69‑151 (MQ=255)

                                                                                ttccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCgg                                                                     >  1:1238163‑M2/69‑151 (MQ=255)

                                                                                 tccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCgg                                                                     <  8:601016‑M2/83‑1 (MQ=255)

                                                                                  ccttgggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGt                                                                    <  2:346155‑M2/84‑1 (MQ=255)

                                                                                      gggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCt                                                               <  7:1106699‑M2/89‑1 (MQ=255)

                                                                                      gggcacttcaccgttttcacgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCt                                                               <  8:942863‑M2/89‑1 (MQ=255)

                                                                                       ggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTg                                                              <  7:628982‑M2/90‑1 (MQ=255)

                                                                                       ggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTg                                                              >  5:858160‑M2/62‑151 (MQ=255)

                                                                                       ggcacttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTAACGGTACGCTg                                                              >  1:369604‑M2/62‑151 (MQ=255)

                                                                                          acttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTAATAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTc                                                            >  5:707648‑M2/59‑150 (MQ=255)

                                                                                           cttcaccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCa                                                           >  2:956530‑M2/58‑150 (MQ=255)

                                                                                              caccgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATg                                                        >  1:206834‑M2/55‑150 (MQ=255)

                                                                                               accgttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGa                                                       >  4:957055‑M2/54‑150 (MQ=255)

                                                                                                  gttttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATTAAATg                                                   >  5:512458‑M2/51‑151 (MQ=255)

                                                                                                    tttcgcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATgg                                                  <  5:381847‑M2/102‑1 (MQ=255)

                                                                                                        gcgctggcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAg                                             <  4:509352‑M2/107‑1 (MQ=255)

                                                                                                              gcctgcgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTc                                         >  3:401842‑M2/39‑149 (MQ=255)

                                                                                                                   cgttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATCTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGAGAGCATCAGtgacg                                  >  2:38998‑M2/34‑149 (MQ=255)

                                                                                                                    gttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTGTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAggg                                 <  4:1016648‑M2/119‑1 (MQ=255)

                                                                                                                    gttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAggg                                 <  1:49608‑M2/119‑1 (MQ=255)

                                                                                                                    gttgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAG‑AATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGa                                <  6:1173263‑M2/119‑1 (MQ=255)

                                                                                                                      tgtcggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGa                                <  8:885820‑M2/120‑1 (MQ=255)

                                                                                                                       gtcggttattacgtggtgtggattgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTAGCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGaa                               <  6:468320‑M2/121‑1 (MQ=255)

                                                                                                                       gtcgggtattacggggtgttgaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGGCAATGAAATGGGCGAGCGTCAGTGAGGGaa                               <  1:1176785‑M2/121‑1 (MQ=255)

                                                                                                                         cggttattacgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATg                            <  5:672154‑M2/124‑1 (MQ=255)

                                                                                                                                 acgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGGCtt                     <  6:516886‑M2/131‑1 (MQ=255)

                                                                                                                                  cgtggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATAGCGGCttt                    >  5:1237482‑M2/19‑150 (MQ=255)

                                                                                                                                   gtggtctggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTAAAGAAATTACCGGTACGCTGACAATGAAATGGGCGAGCGTCACTGAGGGAAATGGCGGCTGTAt                  >  2:802986‑M2/18‑151 (MQ=255)

                                                                                                                                     ggtgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGGCTTTAttt                <  7:175474‑M2/136‑1 (MQ=255)

                                                                                                                                       tgtggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGATATGGCGGCTTTAtttt               >  6:298450‑M2/14‑150 (MQ=255)

                                                                                                                                         tggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGGGAGGGAAATGGCGGCTTTATTTTa              >  6:929128‑M2/12‑149 (MQ=255)

                                                                                                                                          ggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGGCTTTATTTTAATg           >  3:320579‑M2/11‑151 (MQ=255)

                                                                                                                                          ggaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTCCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGGCTTTATTTTAATg           >  7:1209623‑M2/11‑151 (MQ=255)

                                                                                                                                           gaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGGCTTTATTTTAATGc          >  6:874282‑M2/10‑151 (MQ=255)

                                                                                                                                           gaatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGGATTTATTTTAATGc          >  4:700856‑M2/10‑151 (MQ=255)

                                                                                                                                            aatgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGGCTTTATTTTAATGCt         >  1:769987‑M2/9‑151 (MQ=255)

                                                                                                                                             atgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGGCTTTATTTTAATGCTg        >  1:707750‑M2/8‑151 (MQ=255)

                                                                                                                                              tgtatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATAGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGACAGTCAGGGAAATGGCGACTTTAATTTAATGCAgg       >  2:450629‑M2/7‑151 (MQ=255)

                                                                                                                                                tatcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGAGGCTTTATTTTAATGCTGGtg     >  8:668028‑M2/5‑151 (MQ=255)

                                                                                                                                                  tcTTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGGCTTTATTTTAATGCTGGtgat   >  5:1079944‑M2/3‑151 (MQ=255)

                                                                                                                                                    ttAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGGCTTTATTTTAATGCTGGtgatg  >  5:186077/1‑150 (MQ=255)

ACATTTACCCATCTTCGTACCTCATCCACTATCTTGCTCGTAGGTGAGCAGCACTGGCGCAACGCGCTTTAGTCCTTTCTTCTGCCAAAGCTTGCGCTATGCGCGGGGCTTGACCACTTTACTTTGGTTTCGCTGAATTAAGCGAAAATTAAAATAATTCTCTTGCAGGAGAAGGACAATGTATATTTATTGGATTTTATTAGGTCTGGCTATTGCTACAGAAATTACCGGTACGCTGTCAATGAAATGGGCGAGCGTCAGTGAGGGAAATGGCGGCTTTATTTTAATGCTGGTGATG  >  DH1/2204799‑2205096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`