Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA32.8% 149.7 / 31.9 148intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base C (76/8);  new base A (41/0);  total (140/8)
Fisher's exact test for biased strand distribution p-value = 5.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

AAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613631‑613919
                                                                                                                                            |                                                                                                                                                      
         ggCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGATTTTTGAGTGCCAGAAAGATGAAGCACTTTCGCCGACACTGAACGAAAAAGACAGCCGATTATCGGTCAGTTTAAACTTTTGTACGTaaaaaa                                                                                                                                     >  3:1016898/1‑149 (MQ=2)
         ggCTTATCGGTCAGTTTAACCTGGTTTACGTAAAAACCCG‑CTACGGCGGGTTTTTGCTTTTGGAGTGGCAGAAAGAACAAACAGATTCCACGACACAATACAAAAAAGAAACCGACTTATCGGACAGTAAAACCTCTTTAACGTaaaaaa                                                                                                                                     >  8:676385/1‑149 (MQ=2)
             tATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTTGAGGGGCAGAAAGAAGAATGACTGTCCACGACACTAAACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAAACCgat                                                                                                                                >  3:127710/1‑149 (MQ=11)
              aTCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGAGACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAATAAACCGCtg                                                                                                                               >  5:414962/1‑150 (MQ=255)
              aTCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGCtt                                                                                                                               >  1:1051159/1‑151 (MQ=25)
              aTCGGCAAGTTTCCCGTGATTTACGAAAAACCCGG‑CTGCGGCGGGTTTTTGGTTTTGCTGGCGCCCAACTATGATTGACTTGCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCt                                                                                                                                <  7:388178/150‑1 (MQ=2)
               tCGGTCAGTTTCACCTGATTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAACGCAACATATACGTCAATTTAAACAGTTTTACTAAAAAAcaagata                                                                                                                               >  8:1254373/1‑144 (MQ=11)
                 ggTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATTcgg                                                                                                                            >  3:669300/1‑151 (MQ=255)
                   tCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGCTTcgg‑cg                                                                                                                         >  5:334455/1‑151 (MQ=25)
                      gTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGATTTACGTAAAAGAACGCTTccg‑cggcg                                                                                                                      >  7:1084267/1‑149 (MQ=11)
                       tttCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTACGG‑CGGGtt                                                                                                                     >  5:518547/1‑151 (MQ=25)
                        ttCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGG‑CGGGttt                                                                                                                    >  8:1226162/1‑151 (MQ=255)
                         tCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGATTCGG‑CCGGtttt                                                                                                                   >  2:732331/1‑151 (MQ=255)
                         tCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACACGATACGG‑CGGGttt                                                                                                                    >  2:938652/1‑150 (MQ=255)
                          cACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATTCGG‑CGGGtttc                                                                                                                   >  6:1074766/1‑149 (MQ=255)
                           aCCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGttttt                                                                                                                  >  6:1103498/1‑150 (MQ=35)
                             cTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTGTACGTAAAAAACCGCTGCGG‑CGGGtttt                                                                                                                   >  5:74439/1‑147 (MQ=255)
                              tGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAATAACCCGCTGCGG‑CGGGTTTGTCCtt                                                                                                              >  7:1174959/1‑151 (MQ=255)
                              tGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGG‑CGGGTTTATAAtt                                                                                                              >  6:450050/1‑151 (MQ=11)
                                 tttACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGG‑CGGGTTTTTACTTTTg                                                                                                           >  3:841230/1‑151 (MQ=25)
                                    aCGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTGCGG‑CGGGTTTGTCCTTTTgg                                                                                                          >  7:1127426/1‑149 (MQ=255)
                                     cGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGG‑CGGGtttaaaattatgtagg                                                                                                       >  4:1114927/1‑138 (MQ=11)
                                      gTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTGCGG‑CGGGTTTGTGCTTGTGGAgtg                                                                                                      >  7:1061035/1‑149 (MQ=255)
                                       tAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATGCGG‑CGGGTTTTAAATTTTGGAgtgc                                                                                                     >  6:704515/1‑148 (MQ=11)
                                       gaaaacCCCGTCTGCGGCGGGTTTTTGCTTTTGTCGCGGCTACACTATGATGGACTGTCCAACACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAggg                                                                                                      <  7:1092540/150‑1 (MQ=11)
                                         aaaaCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTGTGCTTTTTGAgtgcgc                                                                                                   >  1:956436/1‑146 (MQ=255)
                                          aaccccG‑CGGCGGCGGGTTTTTGCTTTTGGAGGCCCACAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAg                                                                                                  <  5:1105146/148‑1 (MQ=11)
                                           aaCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAATAACCCGATACGG‑CGGGTTTATCATTGTGGAgtgcg                                                                                                    >  4:585802/1‑144 (MQ=11)
                                           aaCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTGTCATTTTTGAgtgggatt                                                                                                 >  8:641411/1‑147 (MQ=11)
                                           aaCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGG‑CGGGTTTCTGATTTTGGAGTGGGAgt                                                                                                 >  3:568009/1‑150 (MQ=11)
                                           aaCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGCTGCGG‑CGGGtttatgattttt                                                                                                           >  2:99245/1‑132 (MQ=11)
                                            aCCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGATACGG‑CGGGTTTGTACTTTTGGAGGGGCAGa                                                                                                 >  8:1210232/1‑150 (MQ=255)
                                             cccG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAAACCGATTCGG‑CGGGTTTTTGCTTTTGGAgtgggcgtt                                                                                                >  3:929378/1‑145 (MQ=11)
                                             cccG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTTCGG‑CGGGTTTTTTATTTTGGAGTGGCAGaa                                                                                                >  5:140805/1‑150 (MQ=255)
                                                  cTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGCTTCGG‑CGGGTTTTTTATTTTGGAgggcgataaattc                                                                                            >  7:778084/1‑140 (MQ=11)
                                                   ttCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCAACGACACTATACACAAAAGAAAGCGGCTTATCGGTCACTTTAACCTGATTTACGTAAAAAACCGATACGG‑CGGGTTTGAACTTGTGGAGTGCCAGAAatata                                                                                           >  4:15242/1‑149 (MQ=11)
                                                    tCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGG‑CGGCTTTTAAATTTTTGAGTGGCattaatacaa                                                                                          >  6:496761/1‑141 (MQ=11)
                                                     cggcggGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGATGCGG‑CGGGTTTATGATTGTGGAGTGGGATAAAGaacaa                                                                                         >  2:346734/1‑146 (MQ=11)
                                                          ggTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGG‑CGGGTTTTTACTTTTGGAGTGGGAGATAGAAGAAAGACtt                                                                                   >  1:355663/1‑150 (MQ=11)
                                                          ggTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATAAGCAAGGGAGAGCGGCGGTGAGAACACATTGCACTGGTTTACGTAAAAACAGGCTTCGG‑CGGGTTTTTAATATTGGAGGGGCAGTACGAGGAAGGACTg                                                                                   >  1:1024969/1‑151 (MQ=2)
                                                            tttttGCTTTTGGAGGCGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAAACCGATACGG‑CGGGTTTATAATTTTTGAGTGGCAGtaattaatatccctttc                                                                                 >  4:749001/1‑137 (MQ=11)
                                                                tGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAATAACCCGCTACGG‑CGGGTTTTAGATTTTGGAGTGCCAGTTATATGATTGAGTGTCcgcg                                                                             >  2:421717/1‑151 (MQ=11)
                                                                  cTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTAATTTTTGAGTGGCAGAAATATAAAAGACAGTCCCCGac                                                                           >  8:947470/1‑151 (MQ=11)
                                                                        gAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATATAACTAAAAACCCCAAGAGT‑CAACTAAATTCATTAGTAGGGGCAGAAAGATGAATGACTGTCCACGacaaaat                                                                      >  5:929202/1‑146 (MQ=2)
                                                                         aGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGG‑CGGGTTTTTACTTTTTGAGTGGGAGAAATATGAAAGACATTCGAAGacacaaac                                                                     >  8:620146/1‑146 (MQ=11)
                                                                             gCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTTGAGTGGCAGAAAGATGACTCTCTGTCCCCGAGACgatccaca                                                                 >  1:267758/1‑145 (MQ=11)
                                                                              cAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCaa                                                                >  7:550052/1‑150 (MQ=35)
                                                                               aGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAAACCGATACGG‑CGGGTTTATACTTTTGGAGTGGGATAAATAAATAACACTTTCCACGacacaaaaaaaaaa                                                               >  6:828924/1‑140 (MQ=11)
                                                                                 aaaGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGATACGG‑CGGGTTTATACTTTTGGAGTGGCAGAAAGAAGAAACACTGTCCCCGACACAATACCaaaa                                                               >  7:1147797/1‑148 (MQ=11)
                                                                                   aGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGTGGGAGAAAGATGAAAGACTGTCCACGACACTATACCAAaaagaa                                                           >  1:1003219/1‑150 (MQ=255)
                                                                                   aGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTGTAATTTTGGAGGGCCAGAAAGAAGAATGACTGTCCACGACACTATACCAAaaagaa                                                           >  8:850127/1‑150 (MQ=255)
                                                                                      tgaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCg                                                       >  5:192801/1‑151 (MQ=35)
                                                                                      tgaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGATTCGG‑CGGGTTTAAACTTTTGGAGTGCCAGAAATATGATACACTTTCCACGACACTATCAAAAAAAGAAAGc                                                        >  8:365779/1‑150 (MQ=11)
                                                                                       gaatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTTCGG‑CGGGTTTTTACTTTTGGAGTGGCAGATATAAGAAAGACTTTCGCCGACACTATACGCAAATGAAAGCgg                                                      >  7:289569/1‑151 (MQ=11)
                                                                                        aatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTGACCTGTTTTACGTAAAAACCCGGTTCGG‑CGGGTTTGTACTTTTTGAGTGGGAGTAAGTTGATTGCCTGTCGGCGACACTAGACCCAAaagcaagctg                                                      >  3:300280/1‑148 (MQ=11)
                                                                                        aatgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATTTACGTAAAAACCCGATTCGG‑CGGGTTTATCCTTGTTGAGTGGCAGATAGAACAGAGACAGTCCACGACACGATAGCAAaaagaaaac                                                        >  8:341802/1‑146 (MQ=11)
                                                                                          tgaCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACACAAAAGAAAGCGGCtt                                                   >  7:690208/1‑151 (MQ=25)
                                                                                                tCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTATACTTTTGGAGGGCCAGAAAGAACAAACACATTCCACGACACCAAACACAAAAGACAGccaattaacgg                                              >  4:93237/1‑140 (MQ=11)
                                                                                                  cACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGG‑CGGGTTTGTACTTTTGGAGTGGGAGAAATATGAATGACTGTCGGCGTCACGATAAGCAAAAGAATGCGGCAGATCGGTc                                            >  3:49084/1‑150 (MQ=11)
                                                                                                  cACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGTGCCAGATAGAGGAATCGCTTTCGTCGACACTATACCCAAAAGAAAGCGGATTATCGGTc                                            >  3:229644/1‑150 (MQ=11)
                                                                                                  cACGACACTATACCCAAAAGAAAGCGGCTTATCGGTAAGTTTAACCTGATTTACGTAAAAAACCGATTCGG‑CGGGTTTTTAATTTTGGATTGGCATAAATAAAAAACACTTTAAACGACACTATAAAAAAAAGAAAACAACATATCGGta                                            >  6:719990/1‑149 (MQ=2)
                                                                                                   aCGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGGAGAAAGAGCTATCGCAGTCCCCGAGACTATCGCCAAAAGCAAGCCGCTTCTCGGTc                                            >  5:58764/1‑149 (MQ=11)
                                                                                                    cGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTAATTTTGGAGGGGCAGAAAGATGAAAGACAGTCCACGACACTATACCAAAAAGAAAGCGGCTGATCGGTAAGt                                         >  7:335901/1‑151 (MQ=11)
                                                                                                      acacTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTTGAGTGGCAGAAAGATGAAACACTGTCCACGACACAATACACAAAAGAAAACGACTTATCGGTCAGta                                        >  8:752706/1‑149 (MQ=11)
                                                                                                        acTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAAACCGCTTCGG‑CGGGTTTGTACTTTTTGAGTGGCAGATATATGAGACCCTGTCCGCGACACGATCGCAAAAAGAAAGCGACATATCGGTCAGtgat                                      >  3:388375/1‑147 (MQ=11)
                                                                                                          tataCCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGATTTACGTAAAAACCCGCTACGG‑CGGGTTTATACTTTTTGAGTGGCAGAAAGATGAAAGACTGTCCACGACCCTATACACAAAAGAAAGCCGCTTTTCCGTCAGTTTTAcg                                   >  2:1162701/1‑150 (MQ=11)
                                                                                                              cccAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGAAGAATGACTGTCCCCGACACTATACGCAAAAGACAGCGGCTTATCGGTCAGTTTTACATGAt                               >  5:1072581/1‑151 (MQ=255)
                                                                                                                 aaaaGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGTGGCAGAAAGATGAATCACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGCTAAGTTTTACCTGATga                             >  2:600886/1‑149 (MQ=255)
                                                                                                                 aaaaGAAAGCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGGTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAAAGACTGTCCACGACACTATCCACAAAAGAAAGCGGCTTATCGGTCAGTGTTAAGTGATGt                             >  6:238337/1‑150 (MQ=11)
                                                                                                                  acagaaacCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGc                                                                                                    <  6:22650/76‑1 (MQ=255)
                                                                                                                   aagaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGGTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGCCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACg                          >  3:1058592/1‑151 (MQ=255)
                                                                                                                   aagaaagCGGCTTATCGGTCAGTTTAACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTAACGCACAGTCATTCCTGATGTACg                          >  1:276301/1‑151 (MQ=14)
                                                                                                                    agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTTGAGGGGCAGAAATATGAAAGACTGTCCACGACACGATAAACAAAAGAATGCGGCTTATCGGTCAGTTTTACATGATCTACGt                         >  6:124032/1‑151 (MQ=14)
                                                                                                                    agaaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGt                         >  8:194801/1‑151 (MQ=40)
                                                                                                                      aaagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCGACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGta                        >  1:535319/1‑150 (MQ=255)
                                                                                                                      aaagAGGCTTATCGGTCAGTTTCACAAGATTTACCTAAAAACCCTCAACGG‑CGGGTTTTTACTTTTGGAGGTGCATAAAGATGAAAGAATGTCCACGACACAATACCAAAAAGAATGCCACTTATCGCTAAGTTTTAAATGATCTACgaa                        >  6:1179529/1‑148 (MQ=255)
                                                                                                                       aagCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTATTTACTTTTGGAGGGGGAGAAAGATCAATGCCTGTCCACGACACTATACCCAAAAGAAAGCAGCTTAACGGTCAGATTTACCTGATGTACGtaat                      >  1:1194027/1‑151 (MQ=14)
                                                                                                                        agCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACGTGATGTACGtaat                      >  4:1233751/1‑150 (MQ=255)
                                                                                                                          cGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTATACCTGATGTACGtaataa                    >  3:522294/1‑150 (MQ=255)
                                                                                                                           ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAc                  >  7:288728/1‑151 (MQ=255)
                                                                                                                           ggCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTAAGTAATAAAc                  >  5:70124/1‑151 (MQ=255)
                                                                                                                            gcatatttttttttttCACGTGTTTTACGT‑AAAACCCGCGTGGGTTTTGTTTTTGGTTTTGCGGGGGGTGATATTCGTATCTCTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAAcc                 <  7:753360/138‑1 (MQ=9)
                                                                                                                              ttATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGt               >  2:1251983/1‑151 (MQ=255)
                                                                                                                                 tCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTcc            >  2:1092449/1‑151 (MQ=255)
                                                                                                                                 tCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTcc            >  8:540028/1‑151 (MQ=255)
                                                                                                                                  cgggccgTTGCACCGGTTTAACGTAAAACCCCGCTGGGG‑CTGTTTTTTGCTTTTGGCGGGGAAGAATGTTGACTGTAGGGCCACGACTCTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcg           <  5:883413/145‑1 (MQ=9)
                                                                                                                                   ggTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcgg          >  7:639855/1‑151 (MQ=255)
                                                                                                                                   ggTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTAACCTGATCTACGTAATAAACCGTTcctg          >  6:955960/1‑149 (MQ=255)
                                                                                                                                     tCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcg        >  4:348270/1‑151 (MQ=255)
                                                                                                                                     tCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcg        >  1:511922/1‑151 (MQ=255)
                                                                                                                                      cAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCcggcgg       >  8:173806/1‑151 (MQ=255)
                                                                                                                                      cAGTTTCACCTGATTTACGTAAAAACCCGCTTTTT‑TTTTTTTTTGATTTTGGTTGGTCAGAAATATTAATTAATTTCCACTACAATATACTCAAATTATAGCTTCTTATCTGTCAGTTTTACCTGATTTACGTAATAAACCGTTCcggcgg       <  2:1090263/151‑1 (MQ=9)
                                                                                                                                       aGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCggg      >  5:1208306/1‑151 (MQ=255)
                                                                                                                                        gTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGt     >  1:853656/1‑151 (MQ=255)
                                                                                                                                         tttCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGtt    >  5:569593/1‑151 (MQ=255)
                                                                                                                                         tttCACCTGATTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACGCTATACACAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGCTTTACGTAATaaagatcggaggcggggt    >  1:417244/1‑136 (MQ=11)
                                                                                                                                          ttCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt   >  1:1204068/1‑151 (MQ=255)
                                                                                                                                          ttCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGttt   >  7:430053/1‑151 (MQ=255)
                                                                                                                                          ttCACCTGATTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCAGAAAGTATCACCTTCTTTAAGTAAAAAAcagatactcaatgttt   >  8:750387/1‑136 (MQ=1)
                                                                                                                                           tCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATAGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTc  >  8:1275265/1‑151 (MQ=255)
                                                                                                                                            |                                                                                                                                                      
AAAGAAAGCGGCTTATCGGTCAGTTTCACCTGGTTTACGTAAAAACCCG‑CTTCGGCGGGTTTTTGCTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTCACCTGTTTTACGTAAAAACCCGCTTCGG‑CGGGTTTTTACTTTTGGAGGGGCAGAAAGATGAATGACTGTCCACGACACTATACCCAAAAGAAAGCGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTC  >  DH1/613631‑613919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑