Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2930T.38.9% 139.9 / 108.8 79intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (42/2);  new base . (28/0);  total (74/5)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-01

TTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATT  >  DH1/936169‑936443
                                                                                                                              |                                                                                                                                                       
ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGAGTCGCTTACTTCACCAAAGTATATTTGGTTGCcgggtggtgata                                                                                                                                >  3:632757/1‑142 (MQ=14)
                                   ggATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACACTAATCCATTACTTCAACAAAAATTAATTCCTTGAGGCGGCCGGATAAGACCCGACCACCTTAGGCTTAAGAGacctaac                                                                                               >  8:282292/1‑144 (MQ=2)
                                             cgacgaCCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTAGTGCTTTACTTCATCAAATTTTAATTGGTTGAGGGGGCCGGATTTGAACCGCCGCGCTTAGGGTTATGAGGCCGACGAGCTACCAGgctg                                                                                  >  1:858426/1‑151 (MQ=14)
                                                         ggTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCcg                                                                        >  7:731011/1‑149 (MQ=40)
                                                                      acgaGCTACCAGGCTGCTCCACCCCGCGTCCCCGTAGTGCGTGACTTCATCAAATTTTAATTCCTTGCGGGGGCCGGATTTGACCCGCCGCCCTTCGGGTTATGAGCCCGCCGAGCTACCACGCTGCTCCCCCCCGCGTCCGTGGATgctc                                                        >  1:76574/1‑149 (MQ=14)
                                                                        gaGCTACCAGGCTGCTCCACCCCGCGTCCCCGTACTGCTTGTCTTCATCAAATTTTATTTGCTTGCGGGGGCGGGATTTGACCCGACGCCCTTCGGGTTATGAGTCCGCCGAGCGACCAGGCTCATGCACAGAGCGTCCGAGGATGCTCAc                                                      >  7:1064806/1‑151 (MQ=14)
                                                                              ccAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCGGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACt                                                >  8:1144498/1‑151 (MQ=255)
                                                                                atgctgctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTcc                                              >  5:361519/3‑151 (MQ=255)
                                                                                aGGCTGCTCCACCCCGCGTCACGGTAGTGCTATAATTCATCAAATTTTAATTGGTTGCGGGGGCGGGATTTGAACCGCCGCCATTAGGGTTATTAGCCCGAAGAGCTACCAGGCTGCTCCAAACAGAGTCCGGGGATGCGCACTATACTcc                                              >  5:1104338/1‑151 (MQ=17)
                                                                                aGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAAGTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCGACCACGCTGCTCCACACCGCGTCCGTGGATGAGCACTATACTcc                                              >  7:314290/1‑151 (MQ=255)
                                                                                aGGCTGCTCCACCCCGCGTCACAGTAATGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCAGGATTTGAACCGAAGACCTTCGGGTTATGAGACCGACGAGCTACCAGGCTGCTCCACACCGCGTCCGTGGATGATCACTATACTcc                                              >  8:851400/1‑151 (MQ=17)
                                                                                  gctgctCCACCCCGCGTCAGCGGAGTCCGTAAATTCAGCAAAATATAATTGGTTGCGGGGGCATTATTAGACCCGACGACCTTCGGCATATGAGACCGACGAACTACCAGGCTGCTCCACCCCGAGTCAGAGGAAGCTCACTATACTCCgg                                            >  4:827458/1‑150 (MQ=9)
                                                                                  cctggtgctcgtcgctTCTCCGTACTGCTTTTCCTGATTAAATTTTCATTGGGTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGc                                            <  3:132370/139‑1 (MQ=17)
                                                                                   ctgctCCACCCCGCGTCACCGTACTGCGTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTAGAACCGACGACCTTCGGGTTATGAGACCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGACTATACTCCGcg                                           >  2:312030/1‑150 (MQ=255)
                                                                                    tgctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATACTCCGCTc                                          >  5:1256571/1‑151 (MQ=255)
                                                                                     gctCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTATTTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACACCGCGTCCGTGGATGCGCACTATACTCCGCTcg                                         >  3:136731/1‑151 (MQ=255)
                                                                                        ccACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGt                                      >  2:30809/1‑151 (MQ=255)
                                                                                          aCCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGttt                                    >  4:1121076/1‑151 (MQ=255)
                                                                                           cctcccgatatcGTACTGCTTTAACTTAATAAAATTTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGtttt                                   <  2:276177/140‑1 (MQ=17)
                                                                                            gcttcggatctgTACTGCTTTTACTTCATAAAATTTCATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  <  6:844798/140‑1 (MQ=14)
                                                                                            cccGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGAAGCCCACAATACAccccacgatatttc                                  >  1:932124/1‑139 (MQ=11)
                                                                                            cccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  >  7:209642/1‑151 (MQ=255)
                                                                                            cccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  >  4:6922/1‑151 (MQ=255)
                                                                                             ccGCGTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCCCACTATACACCGCTCGCGTTTTcg                                 >  3:53362/1‑150 (MQ=14)
                                                                                             ccGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTc                                  >  7:961623/1‑150 (MQ=255)
                                                                                              cgcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCACTATTCTCCGCTcggtatttgat                                >  2:890606/1‑142 (MQ=11)
                                                                                               gcgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCTATATACTACGATCTCGTTTTcctt                               >  5:1085089/1‑148 (MQ=11)
                                                                                               gcgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATg                               >  6:900644/1‑151 (MQ=255)
                                                                                                cgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGGGCACTATACTCCTCTCGCGTTTTCATGc                              >  3:913724/1‑151 (MQ=14)
                                                                                                cgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCCAACTATACTCCGATCGGGTATTCAttc                              >  1:743749/1‑149 (MQ=11)
                                                                                                cgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAAGGGGTATATACTGCCCTCGCGTTTTccttg                              >  1:414890/1‑147 (MQ=11)
                                                                                                cgTCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATTCCCACTATACTACCCTCGCGATTTCATGc                              >  2:113739/1‑151 (MQ=11)
                                                                                                cgTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGc                              >  4:1170461/1‑151 (MQ=255)
                                                                                                  tCACCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACAAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATTCGAAGTATACTACGCTCTAGTTttcattcac                            >  8:550309/1‑150 (MQ=1)
                                                                                                  tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                             >  6:793376/1‑150 (MQ=255)
                                                                                                  tCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCa                             >  4:943696/1‑150 (MQ=255)
                                                                                                    aCCGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATGCGCAGTATACACCGCTCGGGTTTTCATGCAAcc                          >  4:528518/1‑151 (MQ=14)
                                                                                                    aCCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAAcc                          >  1:205139/1‑151 (MQ=255)
                                                                                                     ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCCCTCTATACAGCTAACGCGTTTTCTTGGACCCt                         >  7:1256157/1‑151 (MQ=11)
                                                                                                     ccGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCAGGATGCGAAATATACTCCACAAGGTTTTTCATTCAACCt                         >  6:548227/1‑151 (MQ=11)
                                                                                                     ccGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGCCGACCTTCGGGTTATGCGCCCGACGAGCTCCCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGGTTTCATGCacgct                         >  8:1157292/1‑147 (MQ=255)
                                                                                                     ccGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                         >  3:1039368/1‑151 (MQ=255)
                                                                                                     ccGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCt                         >  2:58726/1‑151 (MQ=255)
                                                                                                      cGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAATGCCACTATAcaacacactcttatt                                   >  1:276701/1‑126 (MQ=11)
                                                                                                      cGTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGAGGATTCGCAGAATACAGCGCACTATTATTCATGCCACCtt                        >  8:56454/1‑151 (MQ=11)
                                                                                                      cGTACTGCTTTTACTTCTTAAATTTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCTCTATACTCCGCTCGCGTTTTCATGCAACCtt                        <  4:728022/151‑1 (MQ=17)
                                                                                                      cGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtt                        >  1:799085/1‑151 (MQ=255)
                                                                                                      cGTAATGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTACACCCCGCGTCCGTGGATGCGCGGTATACAGCGCTATAGTTTTCATGCCCCCtt                        >  3:729423/1‑151 (MQ=11)
                                                                                                       gTACTGCTTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATTCGCACTATACTCCGCACGCTTTTTc                                  >  5:846189/1‑140 (MQ=11)
                                                                                                          cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                    >  2:1168781/1‑151 (MQ=255)
                                                                                                          cTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCtttttt                    >  2:2478/1‑151 (MQ=255)
                                                                                                           tgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGAAGCGCACTATACTCCGATCGCGTTTTCATGGAACCttttttt                   >  3:294995/5‑151 (MQ=17)
                                                                                                           tgcttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGAGGCGCTGTATACTCCCCTCGCGTTTTGATGCAACCgtatttt                   >  1:1207423/5‑151 (MQ=14)
                                                                                                           tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   >  7:630632/1‑151 (MQ=255)
                                                                                                           tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   >  2:84719/1‑151 (MQ=255)
                                                                                                           tGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   >  2:1052612/1‑151 (MQ=255)
                                                                                                           ggCTTTACGTTATTAAAATTTTATTGGGTGCGGGGGGCGGATTTGATGCGACGACCTTCGGGTTATGACCCCGACGAGCTACCAGACTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCttttttt                   <  7:32255/150‑1 (MQ=255)
                                                                                                              ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGATGGAGTTTTCATTCACCCTTTTTTTTAc                >  7:167167/2‑151 (MQ=17)
                                                                                                              ttttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATGCGCTCTATACTCCGCTGGCGTTTTCCTGCAGCCTTCTTTATAc                >  3:480957/2‑151 (MQ=14)
                                                                                                               tttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAc                >  7:833968/1‑151 (MQ=255)
                                                                                                               tttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGGACTATACTCCGCTCGATTTTTCCTGCACCCTTTTTTATAcc               >  1:666759/1‑151 (MQ=17)
                                                                                                               tttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCAGTATACAGCGATCGAGTTTTCATGCAACCGTATTtataac               >  6:410865/1‑149 (MQ=14)
                                                                                                                ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc               >  6:735799/1‑151 (MQ=255)
                                                                                                                ttACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcc               >  5:47764/1‑151 (MQ=255)
                                                                                                                ttACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGTATGCGGTCTATACACCGCTCGGTTTTTCGTGCAACCTGGTTTTTAacc              >  7:1087300/1‑151 (MQ=14)
                                                                                                                 tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc              >  5:1034201/1‑151 (MQ=255)
                                                                                                                 tACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccc              >  6:499448/1‑151 (MQ=255)
                                                                                                                   aCTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAccac             >  6:659415/1‑149 (MQ=255)
                                                                                                                   aCTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGGGGATTCGAACTATACTCCGATCGGGTTTTCATGCACCCTGTTTTTTAacccc            >  4:763229/1‑150 (MQ=14)
                                                                                                                    cTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACTACATTCGGGTTATGAGCCAGACGAGCTACCAGGCTGCTCCACACCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTAcac              >  3:265405/1‑147 (MQ=255)
                                                                                                                    cTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCGGGATGCGAAGTATACAACGATATTGTATTCATTCAACCTGATTTTTAcgaccc           >  1:882574/1‑146 (MQ=11)
                                                                                                                     ttCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCCCCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCACCCTTTTTTTTACcgcg            >  1:244304/1‑150 (MQ=255)
                                                                                                                      tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGAt          >  1:1258495/1‑151 (MQ=255)
                                                                                                                      tcatcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCCCCTTATGAGCCCGACGAGCTACCAGGCTCCTCCAACCCGCGTCCGTTGATGCGCACTATACTCCGCTAGCGTTTTCATGCAACCTTTTTTTTACCCCGAt          >  8:1165115/1‑151 (MQ=25)
                                                                                                                      tcatcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGATGCGGAGTATACTACTATCGATTTTTCATGCAACCTTGTTTTTAACCCGATc         >  1:1170345/1‑151 (MQ=14)
                                                                                                                       catcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGCTc         >  1:1144858/1‑151 (MQ=255)
                                                                                                                       catcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCCTGGAAGCGCACTATACTCCGAAGTCGTTTTCATGCAACCTTTTTTTTACCACGAta         >  4:440566/1‑149 (MQ=17)
                                                                                                                        atcaAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCAGATc         >  6:367084/1‑150 (MQ=255)
                                                                                                                         tcaAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCCCAGTATACTCCGATCTCGTATTCATGGACCCGTGGTTTTACCACGagacgc      >  3:441952/1‑146 (MQ=14)
                                                                                                                           aaaaTTTTATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACgg     <  4:53303/151‑1 (MQ=255)
                                                                                                                           aaaaTTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTa                 <  2:778558/139‑1 (MQ=255)
                                                                                                                              ttttAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGAtt  >  8:793234/1‑151 (MQ=255)
                                                                                                                              |                                                                                                                                                       
TTTTACTTCATCAAATTTGATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCACCGTACTGCTTTACTTCATCAAATTTTAATTGGTTGCGGGGGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCACCCCGCGTCCGTGGATGCGCACTATACTCCGCTCGCGTTTTCATGCAACCTTTTTTTTACCCCGATCACGGATT  >  DH1/936169‑936443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: