Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,065,6180AG28.0% 167.7 / 81.1 117intergenic (+53/‑10)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (52/25);  new base G (25/5);  total (78/39)
Fisher's exact test for biased strand distribution p-value = 1.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.08e-01

Only 100 of 132 total aligned reads displayed.

TAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACTAG  >  DH1/1065471‑1065768
                                                                                                                                                        |                                                                                                                                                       
taacgtaaTTTGCCCCCTGCATCCGTAGCTCAGCTTTATATAGTAATCGGCTACTACCGGTTGGGTCTTAGGTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAA‑Gtt                                                                                                                                                       <  5:927485/150‑1 (MQ=9)
      aagtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTCCGTGATATTGCTCTAGGAGGGGTTTG‑‑AAG‑‑ATGTGCAGGAatatac‑atgaggcgct                                                                                                                                                >  7:1225958/1‑143 (MQ=2)
       agtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAGTACTTGATATTGATATAGTAAGGTAAGG‑‑AAG‑‑ATGAACAGTAAATTAA‑ATATCCCAAt                                                                                                                                                >  6:372191/1‑150 (MQ=11)
         tttCCCTCTGCCTCCGTAGCTCAGCTGTTTAGTCTAATCGGCTACTAACGGTTGGGTCTTCGGTTGGAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAAGTTTTCCCGATg                                                                                                                                               <  4:443628/150‑1 (MQ=2)
          ttCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTATCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTgcc                                                                                                                                                   >  6:986677/1‑144 (MQ=255)
            cccGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACGAGATATTGCGGAAGGATCGGTAGG‑‑AAT‑‑ATCACCAGTAAAATAA‑CTGTCCCGCTGCATcc                                                                                                                                          >  7:1219230/1‑151 (MQ=17)
                ttGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTa                                                                                                                                       <  5:630754/149‑1 (MQ=255)
                   cATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGATCGCTCGGGTCGGAGCGTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCt                                                                                                                                    <  8:17994/150‑1 (MQ=18)
                    aTCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTCCTTGATATGGCTTAAGTAGCGGTATC‑‑AAT‑‑ATCACCAGTAAAAAAA‑ATATCCCGATGCATCCgaatatc                                                                                                                                   >  3:203880/1‑144 (MQ=21)
                    aTCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTTCCGCTGCATGCGtta                                                                                                                                      >  1:443992/1‑145 (MQ=255)
                       cGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTGTTACTTGATATGGCGTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATACGAAGCTcttcg                                                                                                                               >  7:1128623/1‑147 (MQ=255)
                        gTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAA‑GTTTtcccgttccagcct                                                                                                                                        >  8:1059643/1‑130 (MQ=21)
                         gtgttgaGCTGTATAGTGTACTGGGCTACGATCGGATCGGTCTGAGTTTCGTATACTCCCTGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCTTTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTgg                                                                                                                             <  4:323807/145‑1 (MQ=17)
                         gtgCTCATCTGGATAGAGTACTCGGCTACGAACGGAGCGGTCGGAGGTTCTAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTg                                                                                                                              <  4:645676/148‑1 (MQ=255)
                             tCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAaaataa‑at                                                                                                                                                        <  1:422170/120‑1 (MQ=255)
                              cAGCTGGATATTGTACTCGGCTACGAGCGCTTCGGTCGTAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATag                                                                                                                         <  3:1050516/150‑1 (MQ=18)
                                           tACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCGTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACg                                                                                                           >  7:59150/1‑151 (MQ=255)
                                                ggCTACGATCGGACGGGTCGGAGGTTCTAATCCTCGCTGATGCACCATCTGTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCg                                                                                                      <  1:391103/151‑1 (MQ=17)
                                                               ggTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTAATTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAGAATAAGTTTTCCCGATGCa                                                                                                                                             <  3:330898/98‑1 (MQ=11)
                                                                   ggAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑GTTTGCCGCTGCATGCGGTGCGGAGGGGGGTTGCGTACGCGGCTACGGACCGCGCGGTCGGAgggttgcat                                                                                   >  1:569360/1‑144 (MQ=14)
                                                                   ggAGGTTCGAATCCTCCCGGATGCACCATCTCTTACGATATATGGATATAGTAGGGGTAAA‑‑AAT‑‑ATCAGCCTGAAAATAA‑ATATCACGATGCATCCCTAAAAAATAATAATAAAATACTCGGCTAGGACCCGAGCGGTCGGAGGTTCGaac                                                                                   >  2:4740/1‑150 (MQ=9)
                                                                    gAGGTTCGAATCCTCCCGGATGCACCATATGTTACTTGATATGGCTTTAGTAGCGGTTTC‑‑AAT‑‑ATCAGGAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGGTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt                                                                                   >  8:486006/1‑150 (MQ=255)
                                                                      gagtataaTTCTCCCTTATGCAACATCTTTTACTTTATATGGATTTACTAGCTTTATA‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcct                                                                                <  4:174969/145‑1 (MQ=14)
                                                                        ttCGAATCCTCCCGGATGCACCATCTCGTACTTGATATGGAGTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCCTCGGTAGCGCAGCGGGATAGAGTCCTCGGCTACGAACCGCCCGCTCGGAGGTTCGAAtcctcc                                                                              >  7:1271463/1‑151 (MQ=14)
                                                                           gAATCCTCCCGGATGCACCATCTATTACTTGATCTGGCTTTAGTAGCGTTATC‑‑AAT‑‑ATCAGCAGTAAACTAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCgg                                                                           <  3:380989/151‑1 (MQ=255)
                                                                            tatactcgCGGATGCACCCTCTTTTATTAGAATTGTCTTTACTAGTTTTATA‑‑AAA‑‑ATCAGCAGTAAAGTAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGATCGGTCGGAGGTTCGAATCCTCCCgg                                                                           <  4:321124/146‑1 (MQ=11)
                                                                            aaTCCTCCCTTATTCACCATCTCATACTTGATATCTCTATACTAGCTTTATA‑‑AAA‑‑ATCAGCAGTAAAATAA‑TTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGa                                                                          <  4:1065766/151‑1 (MQ=11)
                                                                            aaTCCTCCCGGCTGCACCATCTCTTACTTTATCTGGCTTTAGTAGCGCTATC‑‑AAT‑‑ATCAGCAGTAAACTAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAtcctcc                                                                              <  5:1270691/147‑1 (MQ=255)
                                                                                ctccCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTACCGATTCATACGTAGCAAAGCTGGATAGATTAAGCGGCTACGAACCGACCGGTAGGACGTACGAATACTGCCGGATGcc                                                                      >  6:1163093/1‑150 (MQ=14)
                                                                                 tccCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCAc                                                                     >  1:548656/1‑151 (MQ=39)
                                                                                   ccGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTACCCATGCATCCGTAGCTCAGCTGGATAGAGTACAAGGCAACGAAACCAGCGGTCCGAGGTTAGAAAACTCCCggcttccac                                                                    >  4:482269/1‑143 (MQ=11)
                                                                                       aTGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCACGGATGCACCATAtt                                                               >  6:369935/1‑151 (MQ=255)
                                                                                        tgaacaagctctTACTTCATATGGCTTTCTTAGCGGAACA‑‑AAT‑‑ATCACCATTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGa                                                                                     <  3:1073866/120‑1 (MQ=14)
                                                                                           cccATCTCTTACTTTATTTTTTTTTATTATTTCTCTC‑‑AAA‑‑ATCTTCAGTAAAATAA‑ATTTCCCGATGCATCCGTACCTCATCTGTATAGAGTACTCGGCCAGCATGGGTTGGCTCTTTGGGAGTAATCCTCCCGGATGCACCATATTCTcc                                                           <  8:1110500/150‑1 (MQ=9)
                                                                                             cATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATTCATCCGTAGCTCAGCTGGATAGAGTAATAGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCgt                                                         >  5:802266/1‑150 (MQ=255)
                                                                                                 tctTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATc                                                    >  4:1100810/1‑151 (MQ=255)
                                                                                                     attttatttGGCTTTTTTAGCGTTATA‑‑AAT‑‑ATCAGAATTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGT‑AACCTTc                                                 <  6:239724/142‑1 (MQ=11)
                                                                                                     agtttagcacgCTTAAGTCGCGGTAGC‑‑AAA‑‑TGCTGAATTAAAATTC‑GTTTCCCGACGCATCCGTAGCTCAGCTGTATAGAGTCCACGGCCAGGACCGCTTGGGGCGCCGGGTCTAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTc                                                 <  3:415728/140‑1 (MQ=2)
                                                                                                        tGATACGGCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑CAtatgc                                                 >  1:712573/1‑145 (MQ=14)
                                                                                                          atatGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAAt                                           >  3:817903/1‑151 (MQ=255)
                                                                                                           tacggCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATATTCAGct                                             >  2:135360/4‑149 (MQ=11)
                                                                                                            acggCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTGGTCCGA‑GATAttgatgaat                                           >  8:21550/3‑144 (MQ=17)
                                                                                                             cggCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATAATACTA‑AATATTCAGCAAtt                                          >  1:920770/2‑150 (MQ=14)
                                                                                                              ggCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGAAGCATCCGTAGCTCAGCTGGATAGAGTACTAGGCTACGAACCGAGCGGTAGGACGTTCGAATCCTACCGGATGCACCAAATTATCCGA‑AAACTTCAGCAATTaac                                       >  3:595064/1‑150 (MQ=14)
                                                                                                               gCTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCTA‑GATATTGAGAAAttac                                        >  3:550462/1‑148 (MQ=14)
                                                                                                                cTTTAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACTA‑AATATTCAGAAATGatg                                       >  8:1100530/1‑149 (MQ=14)
                                                                                                                cTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGt                                     >  5:354881/1‑151 (MQ=255)
                                                                                                                 tttAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACAGAGAGGTAGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCAGA‑AATCTTCATCAATTAAGGt                                     >  6:1123883/1‑150 (MQ=14)
                                                                                                                  ttAGTAGCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGt                                     >  4:459796/1‑151 (MQ=11)
                                                                                                                  ttAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTAt                                   >  1:1159606/1‑151 (MQ=255)
                                                                                                                    tttaggtttata‑‑AAT‑‑ATCTGCAGTAAATTAA‑CTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATc                                  <  8:84181/142‑1 (MQ=14)
                                                                                                                    agtagCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGT‑AATCTTCAGCAATGAAGGTATCg                                 >  7:1032719/1‑151 (MQ=37)
                                                                                                                    agtagCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AAACTTCAGCAATGAAGGTATCg                                 >  3:830882/1‑151 (MQ=255)
                                                                                                                     gtagCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATACTCCTA‑AATATTCAGAAATTATGGTaat                                  >  8:152809/1‑149 (MQ=11)
                                                                                                                      tagCGGTATCAAAAA‑‑ATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTTTACGA‑AATATTCCGGAATGaccggatcc                                 >  5:555059/1‑143 (MQ=14)
                                                                                                                       agctttATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGaa                               <  7:349174/145‑1 (MQ=255)
                                                                                                                        gCGGTATC‑‑AAAAAATCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑CATATTGAGGAATGAAGGTATGGa                                >  5:960627/1‑150 (MQ=11)
                                                                                                                            tATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGt                          >  7:381078/1‑150 (MQ=255)
                                                                                                                             aTC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGt                          >  5:1279495/1‑149 (MQ=255)
                                                                                                                              tC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCata                                                                 >  5:228574/1‑110 (MQ=40)
                                                                                                                              tC‑‑AAT‑‑ATCAGAAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGT‑AACCTTCAGCAATGAAGGTATCGAAGAGATAg                        <  8:1030949/150‑1 (MQ=9)
                                                                                                                               atcaaaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGA‑GATCTGCAGGAAGGAGGGTATCGAAGATGt                          >  1:769203/9‑151 (MQ=11)
                                                                                                                                taaaT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCata                                                                 <  6:228145/108‑1 (MQ=255)
                                                                                                                                  aaT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCgg                                                                                                                >  2:550514/1‑61 (MQ=255)
                                                                                                                                  aaT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCgg                                                                                                                <  1:551588/61‑1 (MQ=255)
                                                                                                                                   at‑‑atCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGT‑AAGCTTCAGCAATGAAGGTATCGAAGAGATAGCggt                    >  5:847967/1‑150 (MQ=12)
                                                                                                                                   at‑‑atCAGAAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATCCACCATATTCTCCGA‑AATCTACAGCAAAGAACGTATCGAAGAGGTAGccg                     >  8:318878/1‑148 (MQ=14)
                                                                                                                                   aaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACTA‑AATCTTCCTCAAGTACGGTATGCAAGAGCTAg                        >  5:1181046/5‑149 (MQ=11)
                                                                                                                                   aaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGA‑AATCTTCAGCAATTATGGTATCGAagatctag                        >  1:145719/5‑144 (MQ=11)
                                                                                                                                   aaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTACGA‑AATATTCAGGAAGTATGGTATGGAAGATGTAGCg                      >  4:1080576/5‑151 (MQ=11)
                                                                                                                                   aaaaatCTGCAGTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGA‑AATATTCAGCAATGAAGGTATCGAAGAGGTAGCg                      >  1:108415/5‑151 (MQ=14)
                                                                                                                                    t‑‑atCTGCAGTAAATTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTAt                                   <  1:942341/135‑1 (MQ=255)
                                                                                                                                    t‑‑atCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGT‑AAACTTCAGCAATGAAGGTATCGAAGAGCTAGCggt                    >  5:891282/1‑149 (MQ=255)
                                                                                                                                    t‑‑atCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGa                    >  6:216244/1‑150 (MQ=255)
                                                                                                                                    aaaatCAGCAGTAAACTAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCgg                     <  7:1102457/148‑1 (MQ=255)
                                                                                                                                        tCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAAtt                 >  4:679002/1‑151 (MQ=255)
                                                                                                                                         ctgcagTAAAGTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGGTACGA‑TATATTCAGGAATGAGGGGTTGGAAGAGCTAGAGGAAtt                 >  3:224152/3‑150 (MQ=11)
                                                                                                                                         cagcagTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCAGATCTTCCAATCCAACCTCA‑GCCCCCAATTAACCAATAATCTTCCGGAATGAAGCTATCGATGAGGTACCCGaaatt                >  7:291850/1‑148 (MQ=2)
                                                                                                                                           ggagtaaagtaa‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAAc              <  7:813746/149‑1 (MQ=255)
                                                                                                                                             agTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTa                                                                                                             >  3:1180587/1‑55 (MQ=40)
                                                                                                                                             agTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCg                                                                            >  6:825525/1‑88 (MQ=37)
                                                                                                                                             agTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCg                                                                            <  5:826880/88‑1 (MQ=37)
                                                                                                                                             agTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCg            >  6:724335/1‑151 (MQ=255)
                                                                                                                                              gTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGc           >  4:981953/1‑151 (MQ=255)
                                                                                                                                               tAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGc           >  4:871706/1‑150 (MQ=255)
                                                                                                                                                aaagTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTGTACTA‑GATCTTCAGGAATTATGGTTAGGAAGATCTGGAGGAATTaaaatttc         >  1:1054060/1‑145 (MQ=11)
                                                                                                                                                aaagTAA‑GTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATACGA‑AATCTTCAGCAATGAAGGTATGGAAGAGGTAGAGGAATTAAACGc           >  4:650788/1‑149 (MQ=14)
                                                                                                                                                aaacTAA‑TTTTCCAGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAg         <  2:496432/151‑1 (MQ=255)
                                                                                                                                                aaaaTAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCa          <  5:370451/150‑1 (MQ=255)
                                                                                                                                                aaaaTAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCAGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGT‑AAGCTTCAGCAATGAAGGTATCGAAGAGCTAGCGGAATTAACCGc           >  3:911272/1‑149 (MQ=255)
                                                                                                                                                 aaataa‑atTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAAt                                                                                   <  6:422323/77‑1 (MQ=38)
                                                                                                                                                 aaataa‑atTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAgg        >  1:808296/1‑151 (MQ=255)
                                                                                                                                                  agtaa‑gtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTATCCGA‑AATCTTCAGGAATGAAGGTATCGAAGAGGTAGAGTAATTAAcaggaccc       >  6:826566/3‑144 (MQ=14)
                                                                                                                                                  aataa‑atTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCt                                                                                                              <  6:1287787/49‑1 (MQ=40)
                                                                                                                                                  aataa‑atTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCt                                                                                                              >  5:1289627/1‑49 (MQ=40)
                                                                                                                                                  aataa‑atTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGCTCGCGGTAGTACCCGCatg        >  5:580164/1‑148 (MQ=11)
                                                                                                                                                   gtaa‑gtTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATGCTCCGA‑AATATTCAGCTATGAACGTTACGAAGATCTAGAGGAATTAAcctgagcc       >  3:1114841/2‑144 (MQ=11)
                                                                                                                                                   ataa‑atTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGACCCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCGGGcc      >  5:288168/1‑150 (MQ=255)
                                                                                                                                                        atTTCGCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACTAg  <  3:425501/151‑1 (MQ=255)
                                                                                                                                                        atTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACt                                                                                                                   >  3:530825/1‑39 (MQ=32)
                                                                                                                                                        |                                                                                                                                                       
TAAAGTAAGTTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATCTCTTACTTGATATGGCTTTAGTAGCGGTATC‑‑AAT‑‑ATCAGCAGTAAAATAA‑ATTTCCCGATGCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTACGAACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCACCATATTCTCCGA‑AATCTTCAGCAATGAAGGTATCGAAGAGGTAGCGGAATTAACCGCAGGCACTAG  >  DH1/1065471‑1065768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑