Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH11,647,6690AC23.7% 180.8 / 15.7 150intergenic (+248/+125)dusC/ECDH1_RS08050tRNA dihydrouridine synthase DusC/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (90/13);  new base C (32/0);  total (125/25)
Fisher's exact test for biased strand distribution p-value = 3.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

GCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAATACGCC  >  DH1/1647519‑1647817
                                                                                                                                                       |                                                                                                                                                      
gCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGa                                                                                                                                                        <  8:590854/151‑1 (MQ=31)
gCCGGGGCAGCCCACATGGAGGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGa                                                                                                                                                        <  6:889764/151‑1 (MQ=14)
 ccGGGGCAGCCCCCATGGAGGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGAt                                                                                                                                                       <  4:626514/151‑1 (MQ=14)
       caccccACAGGTAGGGGGGCTGCGGGCGGGTTTAACCGGTATGTAACCGCGCCCCCGACCGGTTAGCCGTATGGTATACGCGGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGgccg                                                                                                                                                 <  7:498354/148‑1 (MQ=11)
       cAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGAGGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGgccg                                                                                                                                                 <  5:453586/151‑1 (MQ=14)
       cAGCCCACATGGAGGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGgccg                                                                                                                                                 <  1:1233845/151‑1 (MQ=14)
        aGCCCACATGGATGGGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGgccgc                                                                                                                                                <  6:763595/151‑1 (MQ=14)
          cccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGC‑GGCGGCGATTGGCCGccc                                                                                                                                              >  7:664022/1‑151 (MQ=14)
          cccACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGCGGGGCCGccc                                                                                                                                              >  6:988281/1‑151 (MQ=9)
            cacaTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGccccc                                                                                                                                            >  4:943978/1‑151 (MQ=31)
               aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTg‑c                                                                                                                                        >  1:198283/1‑151 (MQ=31)
                tGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGCTTGGCCCCCCCCAg‑cg                                                                                                                                       >  2:826659/1‑151 (MQ=9)
                  gATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTg‑cgcg                                                                                                                                     >  2:494088/1‑151 (MQ=31)
                  gATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCG‑CCGCCCCCGTAGCCCTAAGGGGTAAGCCCACGGCCCCGCGCAGCGGCGCATTTGTTAGCCAGAGCCCGGGGGTTCACGGGGCGGGCGCGGATTGGCCGCCCCCTg‑cccc                                                                                                                                     >  4:395949/1‑148 (MQ=11)
                       gggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCt                                                                                                                                >  3:513887/1‑151 (MQ=31)
                        ggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGC‑GGCCGGGATTGGCCCCCCCCTG‑CCCCCGCCtt                                                                                                                               >  4:1250367/1‑151 (MQ=11)
                         gCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGCTTGGCCGCCCCCTG‑CGCGCTCCTTg                                                                                                                              >  5:380393/1‑151 (MQ=14)
                           tGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTgcg                                                                                                                            >  4:719554/1‑151 (MQ=31)
                           tGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCCGCGATTGGCCGCCCCCTG‑CGCGCTCCGTgcg                                                                                                                            >  2:1102598/1‑151 (MQ=14)
                            gAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGC‑GGCGGCGCTTTGGCGCCCCCAG‑CGCGCGCCGTgcgg                                                                                                                           >  7:441167/1‑150 (MQ=11)
                             aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGcc                                                                                                                          >  6:1281353/1‑151 (MQ=14)
                             aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑CGCGGCGATTGGCCGCCCCCTG‑CCCGCGCCATGcccc                                                                                                                          >  1:277868/1‑151 (MQ=255)
                              gggCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGTTTGGCCGCCCCCTG‑CGCCCGCCTTggcccc                                                                                                                         >  2:1076924/1‑146 (MQ=255)
                                 cgcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGGTTGGGGGCCCCCAG‑CGCGCGCCTTgcggccggg                                                                                                                      >  3:362281/1‑145 (MQ=11)
                                 cgcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTg                                                                                                                      >  5:152672/1‑151 (MQ=31)
                                  gcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGC‑GGCGGCGCGTGGCCGCCCCCGG‑CGCGCGCCTTGCGCCCGTg                                                                                                                      >  7:870252/1‑150 (MQ=11)
                                  gcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCCGCGCTTTGCCGCCCCCAG‑CGCCCCCCGTGCGCCAGTg                                                                                                                      >  8:1297606/1‑150 (MQ=11)
                                   cgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGAt                                                                                                                    >  3:729616/1‑151 (MQ=33)
                                   cgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGAt                                                                                                                    >  4:294091/1‑151 (MQ=33)
                                     ttttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGAtt                                                                                                                   >  2:453163/1‑150 (MQ=18)
                                     ttttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGGCAGTGAtt                                                                                                                   >  3:324371/1‑150 (MQ=18)
                                      tttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGCGCCCGGGGGTGGCGCGGGG‑GGCCGCGCGGGGGCGCCCCCCG‑GGCCCACCTTgcggccgggcggct                                                                                                                 >  5:1130550/1‑140 (MQ=11)
                                         aCAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑Tg                                                                                                             >  7:1174076/1‑151 (MQ=37)
                                          cAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGCTTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑Tg                                                                                                             >  5:1188030/1‑150 (MQ=38)
                                           aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCCGGGGC‑GGCGGGGCTTGGCCGCCCCCTG‑CGCGCGCCGTGGGCCCGGGATTTTA‑Tggc                                                                                                           >  3:418876/1‑149 (MQ=11)
                                           aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGCTTATA‑TGa                                                                                                            >  3:1091927/1‑150 (MQ=25)
                                                     ccTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGC‑GGCGGCGCTTGGCCGCCCCCGG‑CGCGCGCCTTGCGCCCGGGAGTATA‑TGACGCCATGgac                                                                                                  >  1:1025329/1‑150 (MQ=11)
                                                       tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGGGCGTTGCCGCCCCCGG‑CGCGCTCCGTGCGCCAGGGCTTATA‑TTAAGCCATGCACATg                                                                                               >  3:938264/1‑151 (MQ=14)
                                                       tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGCTTGGCCGCCCCCTG‑CGCGCGCCGTGGGGCAGTGGTTTTT‑TGAAGCAATGCACATg                                                                                               >  5:690779/1‑151 (MQ=14)
                                                       tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCCATGCACATg                                                                                               >  1:858203/1‑151 (MQ=255)
                                                          cgcCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGCTTGGCCGCCCCCGG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGCAGCAATGCACATGAc                                                                                             >  3:287697/1‑150 (MQ=255)
                                                          cgcCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGAc                                                                                             >  5:399800/1‑150 (MQ=255)
                                                          cgcCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCGTGCGCCAGTGCTTATA‑TGAAGCAATGCACATGAc                                                                                             >  3:72396/1‑150 (MQ=255)
                                                           gcCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGCTTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGAc                                                                                             >  4:472388/1‑149 (MQ=255)
                                                           gcCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGAc                                                                                             >  5:775739/1‑149 (MQ=255)
                                                           gcCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGGGATTATA‑TGAAGCAATGCACATGAc                                                                                             >  8:1170116/1‑149 (MQ=255)
                                                           gcCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCCCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGAc                                                                                             >  5:567388/1‑149 (MQ=255)
                                                              cGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGC‑GGCGGCGCTTTGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TTAAGCAATGCACATGCCacg                                                                                          >  1:282125/1‑147 (MQ=14)
                                                               gACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCACGGGGC‑GGCCGCCAGTGGCCGCCCCCAG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCCCATGCCAGGAgc                                                                                       >  3:58992/1‑151 (MQ=17)
                                                                aCCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGCTTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAgcg                                                                                      >  6:1152555/1‑151 (MQ=255)
                                                                  ccGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAgcgcc                                                                                    >  1:97614/1‑150 (MQ=255)
                                                                   cGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGGCGCCCCCAG‑CGCGCTCCTTGCGGCAGTGCTTATA‑TGCAGCAATGGACCTGCCAGGAgcgcc                                                                                    >  5:912840/1‑149 (MQ=14)
                                                                   cGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATTCACATGACAGGAGCGCa                                                                                    >  4:381781/1‑150 (MQ=255)
                                                                       aGCCGTAAGGGCTACCGCGAGGGCCCCGCGGAGCGGCGATTTTGTGCGCCAGCGCCGGGGGGGGGCGGCGGC‑GGCGGCGATGCGCCGCCCCCGC‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAg                                                                                <  1:368734/150‑1 (MQ=255)
                                                                       aGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAgg                                                                               >  1:470602/1‑151 (MQ=255)
                                                                         ccGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGCTTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGCTTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAggct                                                                             >  3:485153/1‑149 (MQ=255)
                                                                           gTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGGCATTGGCCGCCCCCTG‑CGCCCTCCTTGCGCCAGTGATTATA‑TGAAGGAATGCACATGACAGGAGCGCAACAGGATgc                                                                           >  1:28344/1‑150 (MQ=255)
                                                                            tAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATgac                                                                          >  1:47286/1‑150 (MQ=255)
                                                                                  aTAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CCCGCTCCTTGCGCCAGTTATTATA‑TGAAGCAATGCACAGGACCGGCGCGCAAACGGATgagagccgt                                                                    >  2:95630/1‑147 (MQ=17)
                                                                                    aaGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGC‑GGCGGCGCTTGGGCGCCCCCGG‑CGCGCGCCGTGGGCCAGTGGTTATA‑TTGAGCCATGCCCCTGACAGGCGCGCCACACGCTgcgagccaga                                                                   >  7:517268/1‑145 (MQ=9)
                                                                                    aaGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGCTTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATT‑TGAAGCCATGCACATGACAGGAGCGCAACAGGGTGAGAGACTGa                                                                   >  4:1024708/1‑150 (MQ=255)
                                                                                       ccGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGGCCAACAGGATGAGAGCCTGaattt                                                               >  6:763535/1‑148 (MQ=255)
                                                                                        cGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGGGCCAGTGATTATA‑TTAAGCAATGCACATGACAGGCGCGCAACAGGTTGAGAGACTGAAatat                                                              >  7:1237682/1‑151 (MQ=255)
                                                                                        cGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAatat                                                              >  7:831986/1‑151 (MQ=255)
                                                                                           gggCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCCGGGGC‑GGCGGGGCTTTGCCGCCCCCCG‑CCCGCGCCGTGGGCCCGGGGTTTTATTAAACCAATG‑ACATGACACGCGCGGCAAAAGATGAGAGACGGGAataattt                                                           >  6:1145759/1‑147 (MQ=2)
                                                                                                   cgcAGCGGCGATTTTGTTCGCCAGAGCCCAGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTAttt                                                   >  2:884666/1‑151 (MQ=255)
                                                                                                    gcAGCGGCGATTTTGTTCGCCAGAGCACGGGGGTGCACTGGGC‑GGCGGCGCTTGTCCGCCCCCTG‑CGCCCTCCTTGAGCCAGTTATTAAA‑TGAAGGACTGGACATGACATGAGCGCAACAGGATTATAGACAGAAATATTATAGTtttttt                                                  >  4:342702/1‑151 (MQ=17)
                                                                                                     cAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGC‑GGCGGCGCTTGGCCGCCCCCGG‑CGCGCTCCTTGCGCCAGTGATTATT‑TGAAGCAATGCCCATGCCAGGCGCGGAACAGGCTGCGAGACTGAAATATTCTCGGTAttttg                                                 >  3:926532/1‑150 (MQ=18)
                                                                                                      aGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCCGGGGGC‑GGCGGCGAGTGGCCGCCCCCTG‑CGCGCTCCATGCGCCAGTGATTATA‑TGAAGGAAAGCACATGACAGGAGCGCAACAGGATGACAGACAGAAATATTCTGGTTAtttt                                                  >  4:187551/1‑149 (MQ=18)
                                                                                                             aTTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCGTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCtgt                                         >  3:1188665/1‑150 (MQ=255)
                                                                                                               tttGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCtgatg                                       >  5:413775/1‑151 (MQ=255)
                                                                                                                ttGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCCGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCCTTATTTTCAGACCagatg                                       >  6:1262394/1‑150 (MQ=255)
                                                                                                                    tCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCATTGGCCAGTGATTATA‑TGAAGCAATTCACAAGACAGGAGCGCAAAACGATGAGAGACTGAAATATTGTCGTGTTTTTAGGACCAGATGAAatt                                  >  6:275608/1‑151 (MQ=21)
                                                                                                                     ggcccggcgggggggggGCGGGGGGG‑GGGGGGGTTTGGCCCCCCCCGGCCGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACatt                                  <  8:767190/140‑1 (MQ=255)
                                                                                                                        cAGAGCCCGGGGGTGCAGGGGGC‑AGCCGCGAATGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGCCAGGAGCGCAACAGGATGAGAGACGGAAATATTCACCTTATTTTCCGACATGATGACaataatt                              >  2:667541/1‑151 (MQ=25)
                                                                                                                         agagCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACattaatt                              >  4:735866/1‑150 (MQ=255)
                                                                                                                         agagCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGGTATTTTCGGCCCTGATGACattaatt                              >  7:630241/1‑150 (MQ=255)
                                                                                                                            gCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTaattaatt                          >  2:968491/1‑151 (MQ=255)
                                                                                                                             cccGGGGGTGCAGGGGGC‑GGCGGCGCTTGGCCGCCCCCGG‑CGCGCTCCATGGGCCAGTGATTATA‑TGAAGCAATGCCCAAGACAGGAGCGCCAAAGGATGAGAGACGGAAATATTATCGTTATTTTGGGCCCTGATGGCAATTATTAAttt                         >  6:452917/1‑151 (MQ=25)
                                                                                                                              ggggggggggggggggC‑GGGGTCTTTGGGCCCCCCCCCG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAAtttt                        <  4:163601/140‑1 (MQ=255)
                                                                                                                              ccGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATTCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTAGGACCTGATGACATTAATTAAtttt                        >  4:644195/1‑151 (MQ=255)
                                                                                                                              ccGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGCAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAAtttt                        >  6:1208190/1‑151 (MQ=255)
                                                                                                                              ccGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CCCGCTCCATGCCCCACTGATTATA‑TGAAGCAATGCACATGACACGAGCCCCACAGGATTAGAGACTGAAATATTCTGGTTATTTTAGGACCAGATGACATTAATTAAtttt                        >  6:361483/1‑151 (MQ=37)
                                                                                                                               gggggggggggggggg‑ggggGCG‑TGGGCCCCCCCCCGCCGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTa                       <  1:100435/141‑1 (MQ=255)
                                                                                                                               cGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGCCATTAATTAAtttt                        >  4:808924/1‑150 (MQ=255)
                                                                                                                                ggggggggcgggggg‑ggggggggTGGGGCGCCCCCGGCCGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTa                       <  4:478218/142‑1 (MQ=255)
                                                                                                                                  gggTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTaaa                     >  5:32238/1‑150 (MQ=255)
                                                                                                                                     gggggggggC‑GGGGGTGTTGCGCCCCCCCCGC‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAatat                 <  4:10362/147‑1 (MQ=255)
                                                                                                                                      gCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGAACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCTTTATTTTCGGACCTGATGAAATTAATTAATTTTAAAatat                 >  4:16719/1‑150 (MQ=255)
                                                                                                                                         gggggC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATatcat              >  2:716/1‑150 (MQ=255)
                                                                                                                                         gggggC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATatcat              >  6:278863/1‑150 (MQ=255)
                                                                                                                                             gc‑ggcggcgATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTa          >  4:126051/1‑150 (MQ=255)
                                                                                                                                               gggcggctTTGGGCGGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTaaa        <  3:1071940/150‑1 (MQ=255)
                                                                                                                                                gggggcgGTTGGCCCCCCCCCGCCGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATTTCATCTTaaa        <  3:509933/148‑1 (MQ=255)
                                                                                                                                                ggcggcgATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATTACATTAATTAATTTTAAAATATCATCTTAAAt       >  1:528007/1‑151 (MQ=255)
                                                                                                                                                 gcggcgATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGACGCAATGCACATGACAGGAGCGCAACAGGAGGAGAGACTGAAATATTCGCGTTAGTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAAt       >  1:153291/1‑150 (MQ=255)
                                                                                                                                                 gcggcgATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGCCAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTANTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTANAt       >  1:147528/1‑150 (MQ=255)
                                                                                                                                                      gATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATGATCTTAAATACGcc  >  3:830831/1‑150 (MQ=255)
                                                                                                                                                      gATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAATACGcc  >  8:433500/1‑150 (MQ=255)
                                                                                                                                                       aTTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAATACGcc  >  5:912664/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
GCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGCCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTG‑CGCGCTCCTTGCGCCAGTGATTATA‑TGAAGCAATGCACATGACAGGAGCGCAACAGGATGAGAGACTGAAATATTCTCGTTATTTTCGGACCTGATGACATTAATTAATTTTAAAATATCATCTTAAATACGCC  >  DH1/1647519‑1647817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑