Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,184,5850CA30.8% 164.3 / 37.4 107intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
Reads supporting (aligned to +/- strand):  ref base C (49/25);  new base A (31/2);  total (80/27)
Fisher's exact test for biased strand distribution p-value = 1.69e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

CCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184440‑3184734
                                                                                                                                                 |                                                                                                                                                      
cccGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAATAAAGa                                                                                                                                                    >  7:981745/1‑150 (MQ=255)
   gtcgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTATACAAgcacgg                                                                                                                                                >  1:412398/1‑146 (MQ=255)
     cgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTATTAAAgcggggtc                                                                                                                                              >  1:1255643/1‑144 (MQ=255)
     cgtAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAATCAAgctcggtt                                                                                                                                              >  5:646337/1‑144 (MQ=255)
     cgtAGCCACCAAATTCTGAATGTATAGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTATTCAAGaacgatt                                                                                                                                              >  3:578504/1‑145 (MQ=255)
           caccaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATAAAAGaacccttcggagt                                                                                                                                        >  7:85492/1‑139 (MQ=21)
            accaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAATCAAgctcgagatcgtga                                                                                                                                        >  1:341063/1‑137 (MQ=18)
            accaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTa                                                                                                                                      <  6:683716/151‑1 (MQ=255)
             ccaAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAAGC‑TTAcgtggtc                                                                                                                                      >  5:147273/1‑144 (MQ=255)
              caAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAATAAAGATCC‑GTACCtagaa                                                                                                                                      >  8:478456/1‑145 (MQ=18)
               aaaTTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTAAAAAAGAAGC‑GTACCTAGAAAGt                                                                                                                                   >  7:1071135/1‑151 (MQ=18)
                     tGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTATAAAAGATCC‑TTAcggagttcttgtagcc                                                                                                                             >  7:363302/1‑136 (MQ=18)
                     tGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGACGC‑TTACCTTGTAAGTTAAccc                                                                                                                             >  7:150850/1‑151 (MQ=255)
                              cGAATATTTTCTGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGCTTCCGCGATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGt                                                                                                                    <  7:896355/151‑1 (MQ=255)
                                 atatGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATc                                                                                                                 <  8:226993/151‑1 (MQ=255)
                                   tagtgcatccatttaaaatCCCATTTTTTGGGTTATGGATTAGCTTTAAGTCTCCGTTTTGTGATACCGGCATTCCGTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACATTGTAAGTGCACCCAGTTTGGGTATCg                                                                                                                <  6:682321/135‑1 (MQ=255)
                                    tGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGC‑ATACCTAGAAAGTGAACCAAGTAGGCGTagagac                                                                                                              >  4:202971/1‑146 (MQ=18)
                                             aaTTCAAAACCAATTTGTTTGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTaa                                                                                                     <  8:406173/151‑1 (MQ=255)
                                             aaTTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGAACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTaa                                                                                                     <  2:1208252/151‑1 (MQ=255)
                                                   aaaCCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGG‑TTACGTAGTGAGTGAACCCTGTTGGCGTATCGACAATCTGTAAGGCAcc                                                                                               >  7:490661/1‑151 (MQ=18)
                                                   aaaCCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAG‑CGCGGTACGGGGTGAGTTCAGCGAGTAGGGGTTGCGCCAAGCGGTAAGGcccc                                                                                               >  7:83472/1‑151 (MQ=17)
                                                     gcccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTATGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTAAATTGTAAGTGc                                                                                                                                 >  2:106076/10‑115 (MQ=18)
                                                     gccaggttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGc                                                                                                                                 <  1:106299/106‑1 (MQ=255)
                                                      cccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTc                                                                                                                                                        >  2:498289/9‑92 (MQ=11)
                                                      cccggttattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTc                                                                                                                                                        <  1:499240/84‑1 (MQ=255)
                                                        cggtttttgGGGTATCGCCAAGCGGTAAGGCACCGGTTTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTg                                                                                                                        <  8:860898/115‑1 (MQ=255)
                                                        cggttattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTGTGATACCGGCATTCCCAGGTTCGAATCCACGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTg                                                                                                                        >  7:862326/7‑121 (MQ=17)
                                                        aaTTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACTTTAATAAAGCGGC‑GTTCCGGGTAAGTGGTCCCAGTTTGGGTTGCGACAATCTGGAAGGcccgggtgt                                                                                          >  7:1115722/1‑143 (MQ=2)
                                                           ttattgGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCACAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACCC‑TTACCGTGTAAGTTCAccc                                                                                                                             >  5:499352/4‑113 (MQ=17)
                                                            tattgGGGTATCGCCAAGCGGTAAGGCACCGGATTGTGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAAGC‑TTAACTTTTAAGTGc                                                                                                                                 >  4:79736/3‑108 (MQ=17)
                                                            tattgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGc                                                                                                                                 <  3:79772/106‑1 (MQ=255)
                                                             gttgGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAGCGGC‑GTTCGGAGTGAGTGGTCCCTTTGGGGGTAGCGGCAAGCTGGAAGGCGCGGGAGTCTagt                                                                                     >  5:348289/1‑149 (MQ=2)
                                                                  ggTATCGCCAAGCGGTAAGGCACCGGATTGTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGACGC‑TTACCTTGTAAGTGCAGCCAGTTGGGGTATCGCCaa                                                                                                            >  4:38719/1‑123 (MQ=255)
                                                                  ggTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCaa                                                                                                            <  3:38774/123‑1 (MQ=255)
                                                                   gTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCGTGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAATACGC‑TTACCTTGTAATATCACCCAGTTgg                                                                                                                       >  8:612567/1‑111 (MQ=18)
                                                                       ccgacatccttAAGCCACCTGATTCGGAGTCCGGCTTTACGAGGTTCGTATCCTCGTACACCTGACAATTTATTATAGGCGC‑TTAAATTGAAACTGCACCGTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCAtt                                                                            <  7:707017/140‑1 (MQ=16)
                                                                          cAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGTCATTc                                                                           >  8:68852/1‑148 (MQ=255)
                                                                           aaGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCa                                                                                                                                                       >  4:112533/1‑72 (MQ=14)
                                                                           aaGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCa                                                                                                                                                       <  3:112589/72‑1 (MQ=255)
                                                                            cccgtgaccgctccGGATGCTGATACCGGCATTCCGCGGTTCGAATCCTCGTACCCCAGACAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAgg                                                                      <  5:1067626/139‑1 (MQ=37)
                                                                              cGGTAAGGCACCGGATTCTTATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAATAAAGCAGC‑ATTCGGGGTAGTTGGAGCGTGTGGGGGTAGCGGCAAGCGGTAAGGCCCGGGATACTACTTCCGGCATTCCCAgggt                                                                    >  5:541277/1‑149 (MQ=9)
                                                                                 tAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGAAGC‑TTTCGTTGTAAGTGAACCCATTTTGCGTATCTACAATCGGTAATGCCACGGAGACTAATTACGTCATTCagagtgtcgt                                                                 >  1:674039/1‑145 (MQ=12)
                                                                                  acggatcctgATTGTGATACCGGCATTCCGAGGTTCGTATCCTCGTAATTCAGCATTTTTATTAAAGAGTC‑TTAAATTGTAATTGCAACTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGa                                                                 <  6:335/141‑1 (MQ=12)
                                                                                  aaGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAGGC‑GTACCTTGTAAGTGCACCGAGTTGGGGTATCGCCAAGCGGGAAGGCACGGGAGTCTACTTGAGGCATTCCGAGTGTCGa                                                                 >  5:1233528/1‑150 (MQ=25)
                                                                                  aaGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAGGC‑GTACCTTGTAAGAGCACCAAGTTGGTGTATCGCCCAGCGGTAAGCCACCGGATTCTAATTCCGGCATTCCGAGGTTCGa                                                                 >  3:1066018/1‑150 (MQ=37)
                                                                                    gccACCGGATGCTGAAACCGGCAATCCGAGGTTCGAATCCTCGTACCCCAGCAAATTTATTCAAGACGC‑TTACATTTTAACCGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATc                                                              <  7:109200/149‑1 (MQ=25)
                                                                                        ccGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGC‑GTACCTAGTAAGTGCACCCAGTTTGCGTAGATCCAAGCTCTAAGGCCAAGTATTCAAATGGGGTCATTCAGAGGTGCGTGTCCTCg                                                          >  7:144066/1‑151 (MQ=12)
                                                                                        ccGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATAAAAGACGC‑TTACCTTGTAAGTGAACCCAGTTGGCGTATCGCCAAGCGGTAAGGCCACGGAGTCTAATTCCGGCATTCAGAGGATGGAATCCTCg                                                          >  5:429330/1‑151 (MQ=37)
                                                                                        aaggttgcctcaaccGGCAGTCCGACGTTCGCATCCTCGTACATCATATTTTTTATTCATTTTGC‑TTACATTGTTAGTTCAACTATTTGGGGTATCGCTAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCt                                                            <  4:910048/136‑1 (MQ=2)
                                                                                         cGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTAAAGCGGG‑TTACGTGGTAAGTGAACCCAGTTGGGGTATCGCCAAGCGGTAAGGCGCAGGATTCTAATGGCGGCATTCagagtagcgagtcatcgt                                                         >  7:827913/1‑137 (MQ=21)
                                                                                          ggATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAGCC‑GTACCTGGTAAGTGCACCGAGTGGGGGTAGCGGCAAGCGGTAAGGCCCCGGATTCTAATAGCCGCATTCAGAGTTGCGacgccagct                                                         >  1:718245/1‑142 (MQ=21)
                                                                                           gctgctgacaccGGCACTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGCCTC‑TTAACTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTAc                                                       <  7:685624/141‑1 (MQ=25)
                                                                                              gctgagaccGGCAATCCTAGGGTCGTATCCACGTACCCCAGACAATATCTACAAGCCTC‑TTCACTTGTAAGTGCAACCTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTAccc                                                     <  7:628597/143‑1 (MQ=17)
                                                                                               gtGACTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGCCGC‑TTAACTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCa                                                   <  7:938945/150‑1 (MQ=255)
                                                                                               cTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAGGC‑TTTCGTTGTGAGTGATCCGTGTTGGGGTTTCGGCAAGCGGTAAGGCGACGGATTCTAATTGCGTCATTCCGAGTGTCGAATGCGCGTcccgcc                                                   >  1:706960/1‑148 (MQ=19)
                                                                                                tGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTAATAAAGAACC‑ATACCTTGTAATTTAACCAATTATGGGTAAATACAATCTGTAATGCACAGTATTCAAATTCAATCATTCCTAGTTTCAAATcaacctccccc                                                    >  4:667450/1‑139 (MQ=9)
                                                                                                         cacgccGAGGCTCGAATCCACCTACCCCAGCCAATATATTCAATTTTC‑TTATATTTTAATTGCACTCATTTGTGGTATCGACAATCTGTAAGTCACATTATTATAATTACGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTaa                                         <  4:1054174/147‑1 (MQ=12)
                                                                                                           cgcctcccgtCGAAGCCACGAACCCCAGACACTTTATACAATCCGC‑GTACCATGCAATAGCAACTAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTaaaa                                       <  5:921385/142‑1 (MQ=14)
                                                                                                             ccGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGACAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTaaaaa                                      >  4:1276330/1‑150 (MQ=255)
                                                                                                              cGAGGTTCGAATCCTCGTACCCCAGCCAAATTATTAAAGCCCC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGACAAGCGGGAAGGCACGGGATTCTAATTCGGGCATTCCGAGGTTCGACTCCTCGTCCCCCAGCGAAATTaaaaaac                                    >  3:503883/1‑150 (MQ=255)
                                                                                                               taGGTTCGAATCCTCGTACCCCAGCCAATTTATACAAGACGC‑TTACCTTGTAAGTGAACCCAGTTGGGGTATCGACAATCGGTAAGGCACAGGATTCTAATTAAGGCATTCCAAGTATCGAAACATCGTACCCCAGCCACATTAAAAAAGc                                   >  6:1265628/2‑151 (MQ=255)
                                                                                                               gAGGTTCGAATCCTCGTACCCCAGCCAATTTAAAAAAGCCGC‑TTACCTGGTAATTGAACCCATTTGGGGTATCTCCAATCGGTAAGGCACAGTAGTCTAATTACGGCATTCCGAGGATCGAATCCACGTCCCCCAGCCAAATTAAAAAAGc                                   >  8:214319/1‑151 (MQ=255)
                                                                                                                aGGTTCGAATCCTCGTACCCCAGCCAAATTATTCAAGCCCC‑TTACCTGGTAAGTGCACCCAGTTGGGGTATCGCCAAGCTCTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAAGCCTCGTCCCCCAGCCACATTAAAAAAGCt                                  >  2:839266/1‑151 (MQ=25)
                                                                                                                  gTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGAGGC‑TTACCTTGTAAGTGAACCCAGTTGGGGTATAGCCAAGCGGTAAGGCACAGGATTCTAATTCCCGCATTCCGAGGTTCGAATCAACGTACCCCAGCCACAATAAAAAAGCt                                  >  3:1181306/1‑149 (MQ=255)
                                                                                                                  gTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGTTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCg                                >  1:350340/1‑151 (MQ=255)
                                                                                                                    tCGAATCCTCGTACCCCAGCCAAATTATTAAAGCCCC‑GTACCTTGTAAGTTAACCGAGTTTGGGTAAATACAAGCGCTAATGCACAGGATTCTAATTCAGGCATTCAGAGTATCGAAACATCCTACCGCAGCAAAATTAAAAAAGCTCGCt                              >  2:1051113/1‑151 (MQ=255)
                                                                                                                     cGAATCCTCGTACCCCAGCCAATTTATTCAAGCAGC‑TTACCTTGAAAGTGCACCCAGTTGGGGTATAGCCAAGCTCTAAGGCACAGGATTCTAATTACGGCATTCCGAGGATCGAATCCACGTACCCCAGCAACATTAAAAAACCTCGCt                              >  8:1108730/1‑150 (MQ=255)
                                                                                                                     cGAATCCTCGTACCCCAGCCAATTTATTAAAGCACC‑GTACCTAGTAAGTGAACCCAGTTGGCGTATCGACAATCTGTAAGGCACCGGATACTAATTAAGGCATTCCGAGTATCGAAACCTCCTACCCCAGCCACATAAAAAAACCtatct                              >  6:1193301/1‑146 (MQ=255)
                                                                                                                     cGAATCCTCGTACCCCAGCCAATTTATTAAAGAAGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATAGACAAGCGGTAATGCACCGGTTTCTAATTCCGGCATTCAGAGGTTCGAATCCTCGTACCGCAGCCACATTAAAAAAGCTCGCtt                             >  8:473190/1‑151 (MQ=255)
                                                                                                                      gAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTc                            >  5:767134/1‑151 (MQ=255)
                                                                                                                      gAATCCTCGTACCCCAGCCAATTTATTAAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTCCCCCAGCCACATTAAAAAAGCTCGCTTc                            >  3:1191645/1‑151 (MQ=255)
                                                                                                                       aaTCCTCGTACCCCAGCCAAATTATTCAAGACGC‑TTACCTAGTAAGTGCACCGAGTAGGGGTATCGCCAAGCGCTAAGGCAAAGGATTCTAATTCCAGCATTCCGAGTATCGAAACCACGTACCCCAGACACAATAAAAAACCTATCTTcc                           >  2:659366/1‑150 (MQ=255)
                                                                                                                        aTCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCgg                          >  4:863130/1‑151 (MQ=255)
                                                                                                                        aTCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGCTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTAGCTTCgg                          >  2:448383/1‑151 (MQ=255)
                                                                                                                        aTCCTCGTACCCCAGCCAATTTATTAAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCgg                          >  3:1133612/1‑151 (MQ=255)
                                                                                                                          ccTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTTGGGTATAGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGTTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  3:1234635/1‑151 (MQ=255)
                                                                                                                          ccTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGTTTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  3:749137/1‑151 (MQ=255)
                                                                                                                          ccTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  2:1009659/1‑151 (MQ=255)
                                                                                                                          ccTCGTACCCCAGCCAATTTATTAAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCTCCAAGCGGTAATGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCATCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCg                        >  4:351541/1‑151 (MQ=255)
                                                                                                                             cGTACCCCAGCCAATTTATTCAAGACGC‑TTAGCTTGTAAGTCCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGTTTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTAGCGCAGCCACATTAAAAAAGCTCTCTTCGGCGAGc                     >  5:863377/1‑151 (MQ=25)
                                                                                                                              gTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCt                    >  3:135270/1‑151 (MQ=255)
                                                                                                                              gTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCt                    >  4:689042/1‑151 (MQ=255)
                                                                                                                              gTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCt                    >  6:419692/1‑151 (MQ=255)
                                                                                                                               tACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtt                   >  7:295728/1‑151 (MQ=255)
                                                                                                                                aCCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttt                  >  2:1167129/1‑151 (MQ=255)
                                                                                                                                 ccccAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtttt                 >  7:1177605/1‑151 (MQ=255)
                                                                                                                                 ccccAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCtttt                 >  5:1273030/1‑151 (MQ=255)
                                                                                                                                  cccAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGAACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCttttt                >  2:768270/1‑151 (MQ=255)
                                                                                                                                     aGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCt             >  4:723955/1‑151 (MQ=255)
                                                                                                                                       ccAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCttt           >  4:724994/1‑151 (MQ=255)
                                                                                                                                        cAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          >  7:73892/1‑151 (MQ=255)
                                                                                                                                        cAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          >  8:170532/1‑151 (MQ=255)
                                                                                                                                        cAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCtttt          >  7:530302/1‑151 (MQ=255)
                                                                                                                                        cAATTTATTCAACACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCTGCATTCCCAGGTTCGAATCCTCGTACCCAAGCCACATTAAAAAAGCTCGATTCGGCGAGCTTTTATCtttt          >  6:688394/1‑151 (MQ=25)
                                                                                                                                          aTTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCt        >  5:1177241/1‑151 (MQ=255)
                                                                                                                                            ttATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGc      >  6:261197/1‑151 (MQ=255)
                                                                                                                                              aTTCAATCCGC‑TTAAATTGTAAGAGCAACTAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCg     <  6:147016/150‑1 (MQ=21)
                                                                                                                                                tCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTAGGCGAGCTTTTTGCTTTTCTGCGttc  >  1:73383/1‑151 (MQ=255)
                                                                                                                                                 cAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  2:707879/1‑150 (MQ=255)
                                                                                                                                                 cAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGttc  >  4:604083/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                      
CCCGTCGTAGCCACCAAATTCTGAATGTATCGAATATGTTCGGCAAATTCAAAACCAATTTGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTGATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCAATTTATTCAAGACGC‑TTACCTTGTAAGTGCACCCAGTTGGGGTATCGCCAAGCGGTAAGGCACCGGATTCTAATTCCGGCATTCCGAGGTTCGAATCCTCGTACCCCAGCCACATTAAAAAAGCTCGCTTCGGCGAGCTTTTTGCTTTTCTGCGTTC  >  DH1/3184440‑3184734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑