Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | DH1 | 3,448,523 | 0 | A | C | 25.1% | 584.5 / 48.4 | 283 | intergenic (‑83/+73) | malZ/proY | alpha‑glycosidase/proline‑specific permease ProY |
Reads supporting (aligned to +/- strand): ref base A (145/67); new base C (0/71); total (145/138) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.39e-28 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 302 total aligned reads displayed.
ATGAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGA > DH1/3448373‑3448672 | gAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggagg < 7:919226/151‑1 (MQ=255) gAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGggaggagg > 5:774877/1‑151 (MQ=255) accacgtGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGACATTTGGGggaggagga < 8:238143/145‑1 (MQ=255) gCAGTTGGTCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGAc < 4:68720/151‑1 (MQ=255) aGTTGATCTTTGCTTTGTTTACCAAATGGGGGCACCCGCAGGTGCCATGCATTTAACATCAAAATTCCCCTGTGATGAAAATATGATCACCTTGCCATAGCCTATTGCATGATCACTCATCAACTGAAGCATATTTGGGCGAGGAGGACgg > 2:360960/1‑151 (MQ=255) aGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACgg < 4:915009/151‑1 (MQ=255) gTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggg > 8:108382/1‑151 (MQ=255) ttGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACggg < 8:1115336/150‑1 (MQ=255) gATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGc > 1:274235/1‑151 (MQ=255) gATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGc > 2:1012871/1‑151 (MQ=255) gATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGCGGGc > 7:405441/1‑151 (MQ=255) tttgcttttTTTAACAAATGGGGGCCCCGGCAGGGGCAATTCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATCCAGGATCACTCATCATCTCAAGTATATTTGGGGGCGGAGGACGGGGGCGGa < 6:1118102/150‑1 (MQ=255) tgctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATa > 1:311593/1‑150 (MQ=255) gctttgTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAAc > 3:85713/1‑151 (MQ=255) ttgTTTAACAAATGGGGGCACCGGCAGGTGCCATTCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGTTTATTTGGGGGAGGAGGACGGGGGCGGATAAc < 1:1064011/148‑1 (MQ=255) gTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCg > 7:772734/1‑151 (MQ=255) gTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCg > 4:589821/1‑151 (MQ=255) taaaaCAAATGGGGGCACCGGCCGGTGACATTCATTTAAAATAAAAATTCCCCTTTGATGAAAATCTCATCACCTTGCCATAGCCTAATCCAGGATCACTCATCATCTGAAGGATTTTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCg < 2:1159348/147‑1 (MQ=255) ttAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCgg > 6:947873/1‑150 (MQ=255) aaCAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAAt > 6:483341/1‑151 (MQ=255) aactGGGGTCACCGGCAGGATCAATTCATTTAAAATAATAATTGCCCTGTGATCAAAATCTTAGCACCTTGCCATAGCCTAATCCATCATCACTCATCATCTGAAGGATATTTGGTGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGc < 8:1270262/148‑1 (MQ=255) aaaTGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGc > 1:1135219/1‑151 (MQ=255) gtgggCACCGGCATGTGAAATGATTTTAACATCATAATACGCCTTTGATTAAAATCTGACCACCATGCCTTATACTAATGCAGGATCACTCTTCATGTGTCGGTTATTTGGGGGCGGAGGCCGGGCGGGGATAACTTTCGGAATAGCCgg < 5:150582/148‑1 (MQ=255) gggggCACCGGCATGTGCCATTCTTTTAACATCATAATTCCCCTGTGATTAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTTAAGTATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCgg < 8:749686/150‑1 (MQ=255) ggggCACGGGCAGGTGACATGAATTAAAAATATTAATTCCCATGTGATCAAAATGTTATCACGTTGCCATACCATAATGCAGTATAACTCATCATATTATTTTTTTTTGGGGTAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGAt < 5:538106/151‑1 (MQ=255) gCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATc > 6:208747/1‑149 (MQ=255) gCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATc > 5:909472/1‑149 (MQ=255) cACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAgg > 1:902738/1‑151 (MQ=255) cACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATCAAAATCTTATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTTAATGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAgg < 7:970981/151‑1 (MQ=255) ccGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAt > 5:1199834/1‑151 (MQ=255) cGGCCGGTGCCATTCATATAACATCATAAGTCCCCTGTGATTAAATTGTGAGCCCCTTGCCATAGCCTACTCCAGGATCACTCATCTTCTGAAGGTTATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAtt < 2:1223477/151‑1 (MQ=255) cGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAtt > 4:1013438/1‑151 (MQ=255) cGGCAGGTGCCATGCATTTAACATCATAAATCCCCTGTGAAGAAAATCTGATCCCCTTGCCATACCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAtt < 5:81999/151‑1 (MQ=255) ggCCGGGGCCATGAATTAAACATCATAAATCCCCTGTGATCAAAATCTGATCCCCTCGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGAttt < 4:79079/151‑1 (MQ=255) gaGGTGAGATTCATTTAACATCATAATTCCGCTGTGATCAAATTCTGTTCAGCTCGCCATAGGTAAATTCAGGTTTAGTCATATTCTCAAGTTTATTTGGGGGCGGCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtc < 7:667239/149‑1 (MQ=255) cAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTtcc > 4:999128/1‑151 (MQ=255) gCCATGCATATAACATCATAATTCCCCTGTGATGAAAATCATATCACCTTGCCATAGCCTATTCCAGGATCACTCATAATCTGACGGTTATTGGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTg < 3:1129648/151‑1 (MQ=255) ccATGCATTTAACATCATAAATCCCCAGTGATGAAAATCTGATCACCTTGCCATACCCTAATGCACGATCACACATCATCTGCAGGATATTTCCGGGAGGAGGAGGGGGGCGGATAACTTTAGGAATAGCCCGATCAGGAGATCATCCTgc > 2:605381/1‑150 (MQ=255) gCATTAAACATCATAATTCCCCTGTTATGAAAAGCTGATCACCTTCCCATAGCCTAATGCAGGATCACTCATCCTCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAcc < 5:127130/150‑1 (MQ=255) ccATTTAACATCATAATCCCCCTGTCATCAAAATCTGATCACCTTGCCATAGCCTACTGCAGGATCACTCATCCTCTCAAGCATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCg < 4:502818/150‑1 (MQ=255) aTTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGc > 7:615022/1‑151 (MQ=255) aTTTAACACCATAATTCCGCTGTCATGAAAATCTGTTCACCTTGTCATAGCCTCTTCCAGCATCACTATTCTTGTGACGGATATTGGGGGGCGCCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGc < 6:973901/151‑1 (MQ=255) tttAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCt > 3:20693/1‑151 (MQ=255) tttAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACGTTCGGAATAGCCGGATCAGGATTTCCTCCTGACCCGGCt > 4:129723/1‑151 (MQ=255) taaaCCTCATAATTCCCGTGTTATGAAAATCTGATCACCTTCCCATACCCTAATCCAGGATCCCTCATCCTCTGAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCt < 6:331975/148‑1 (MQ=255) aaacacaataagaccccTGTGATCAAACCCTGAGCACCTCCCCATACCCTCAGCCACGACCCCTCATCATCTGAAGGATCTTTGGCGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTa < 5:745736/138‑1 (MQ=255) aaCATCATAATTCCCCTGTGATCAAAATCTGACCCCCTTGCCATAGCCTAATGCCGGAGCACTCATCATCTTAAGTATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc < 2:323510/151‑1 (MQ=255) catcatAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTAc > 1:50155/1‑149 (MQ=255) catAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAAc > 3:786827/1‑151 (MQ=255) aaaaTTCCCATGTGCACAAATTCTCAACCCCTGCCCAAAGCCCAATCCAGGATCACTCCTCATCTCTAGGATTTTTGGGGGCCCCGGCGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACg < 7:154478/149‑1 (MQ=255) tAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGc > 1:437491/1‑151 (MQ=255) aaTTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGc > 1:1261161/1‑150 (MQ=255) ttCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCa > 1:1102614/1‑149 (MQ=255) tCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTACTCCTGAACAGGCTTACAGAACGCAAgg > 6:879003/1‑151 (MQ=255) gccccTGTGAGTAAAATCTGATCACCCTGCCATCGCCTAATGCCGGATCACTCATCATCTCAAGGATATTTGGGGGCGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAgg < 5:349541/150‑1 (MQ=255) cccccTGTCATGAAAATCTGAGCACCTGCCCATACCCTAACGCAGCACCACTCCTCATCTGACGCATATTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAgg < 5:985544/150‑1 (MQ=255) cccTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGAt > 2:649057/1‑151 (MQ=255) ttgatgaAAATCTGAACACCTTCCCATAGCCTAATGCAGGCTCACTCCTACTCTGAAGGATATTTGGGGCTGCCGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTa < 2:209646/148‑1 (MQ=255) tgatgaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCGTCCTGAACCGGGTTACAGATCGGAGGGATTACTg > 4:406780/1‑151 (MQ=255) gatgaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGACCCGGCTTACAGAACGCAAGGATTACTgg > 5:686819/1‑151 (MQ=255) gacgaAAATATCCTCACCTTGCCATACCCTAATGCTTGATCAGTCATCATGTGTAGTTTATGTGTGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTg < 6:1137069/147‑1 (MQ=255) tgaAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGtt > 4:290055/1‑151 (MQ=255) aaaagctcgccACCTTGCCATCCCCTAATGCAGGACCCCTAATCATCTGAAGGATATTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGttt < 7:849141/140‑1 (MQ=255) aaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc > 2:86315/1‑151 (MQ=255) aaaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc > 5:1289972/1‑151 (MQ=255) aaaTCTGATCACCTAGCCATAGCCTAATGCAGGACCACTCATCATCTGAAGGATATTTGGGGGCGCAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTc < 3:234013/151‑1 (MQ=255) aaTCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGCGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGACTTACAGAACGGAAGGA‑TACTGGTTTTc > 5:643304/1‑149 (MQ=255) cccTGGCCATCGCCTACTGCAGGAGCACTCATCATCTGAAGGATATTTGGGGGCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGa < 3:758031/150‑1 (MQ=255) ccTTGCCAGAGCCTAATCCCGGAGCCCTCCTCATCTGAATGATATGCGGGGGCGGCGGCGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGAc < 5:239449/151‑1 (MQ=255) ttGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACg > 3:143132/1‑150 (MQ=255) gtGCCAACGCATAATCCAGCAGCACTCATCACCTGCAGGCTCGTTGGGGCAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGa < 5:1023914/150‑1 (MQ=255) tGCCATAGCCTACTGCAGGATCACTCATCATCTGAATTATATTTGGGGGAGGAGGCGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGa < 8:1052299/150‑1 (MQ=255) tGCCATAGCCTAATGCAGCAGCACTCATCATCTGAAGGATATTTGGGGGCTGAGGCGGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGa < 4:1167818/150‑1 (MQ=255) gCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATc > 1:472065/1‑151 (MQ=255) ccatccccTAATCCAGGAGCACTCATCATCTGAAGTATTTTTGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCg < 6:681450/145‑1 (MQ=255) cataatggcgtaTCATTCACCATGTTAAGTATTTTTGGGGCCGGAGTCCGGGCGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAAGCGGCTTAGAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTggcgg < 4:235058/140‑1 (MQ=255) gCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGCCGATCGTGGCGGCGttt > 3:105513/1‑150 (MQ=255) gCAGGAGCACTCATCATCTCAAGGATATTTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGttt < 7:1259763/150‑1 (MQ=255) ggATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGACCGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAAc > 4:196459/1‑151 (MQ=255) cACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATcc > 8:684141/1‑151 (MQ=255) ccTCATCATCTGAAGGATATGGGGGCGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCa < 5:1103423/150‑1 (MQ=255) aCTCATCCTCTGAAGGATATTTGGGGGCGGCGGCCGCGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCa < 1:180051/151‑1 (MQ=255) aCTCATCATCTGAAGGATTTTTGGGGGCGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCa < 4:85673/151‑1 (MQ=255) cgcatcatCTGAAGGATATTTGGGGCCGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAg < 3:562770/149‑1 (MQ=255) cTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATcc > 7:1036058/1‑149 (MQ=255) cctcatcTGAAGGATATTGGGGGGAGGAGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGcc < 1:329241/149‑1 (MQ=255) catcctCTGAAGGATATGTGGGGGAGGCGGCCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAg < 2:975586/149‑1 (MQ=255) tcatcTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCa > 3:328504/1‑150 (MQ=255) tGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc > 8:1157953/1‑151 (MQ=255) tGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAAc > 8:282919/1‑151 (MQ=255) agtatctttGCGGGCCGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCACCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGc < 5:659722/145‑1 (MQ=255) aGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGc > 8:403358/1‑151 (MQ=255) ggATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGc > 2:546424/1‑150 (MQ=255) tatTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGcaca > 1:1190387/1‑150 (MQ=255) ttGGGGGCGGCGCGCGGGGCCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaaca < 2:49959/150‑1 (MQ=255) ttGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCacaaca > 5:42174/1‑150 (MQ=255) ggaggcggcCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAc < 1:1007364/151‑1 (MQ=255) ggaggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAc < 7:1179860/151‑1 (MQ=255) aggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCAcgc > 8:42553/1‑151 (MQ=255) ggaggaCGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCAAcgcc > 7:1274675/1‑150 (MQ=255) | ATGAGCAGTTGATCTTTGCTTTGTTTAACAAATGGGGGCACCGGCAGGTGCCATGCATTTAACATCATAATTCCCCTGTGATGAAAATCTGATCACCTTGCCATAGCCTAATGCAGGATCACTCATCATCTGAAGGATATTTGGGGGAGGAGGACGGGGGCGGATAACTTTCGGAATAGCCGGATCAGGATTTCCTCCTGAACCGGCTTACAGAACGCAAGGATTACTGGTTTTCAGCCAGCTGACGATCGTGGCGGCGTTTAAACATCCAGCCAATCAACAGCACAACAATCCACGCGA > DH1/3448373‑3448672 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |