Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,122,1240CA25.3% 267.8 / 11.5 175intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (83/26);  new base A (37/0);  total (148/27)
Fisher's exact test for biased strand distribution p-value = 3.05e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

CTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121977‑4122272
                                                                                                                                                   |                                                                                                                                                    
acaaaaacccccatATTCGCGCGGGGGGGGTGTGTCCATTATATCACTTGCACATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCttt                                                                                                                                                   <  5:230376/138‑1 (MQ=14)
   ccAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTGAAATTTTGGCGGGGGAAACGAGACTCGAACTCGCGCCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGGGGTCTGTGTTTTgg                                                                                                                                                 >  7:454502/1‑149 (MQ=14)
      cctcggatATTCCCGGTGGGGTGGTGTGTGCCTTAAAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTgcagca                                                                                                                                             <  7:928264/144‑1 (MQ=18)
       cggagaTATTCCCGCTTGGGGGGTGTGTCCCTTAAAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCAc                                                                                                                                            <  5:644222/149‑1 (MQ=18)
          aGCTATTCGCGCTTGGGTGGTCTGTTCCTTACATTACTTTAAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACtt                                                                                                                                          <  6:1020899/150‑1 (MQ=255)
             tATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGGTTTGGAGCACTTTTc                                                                                                                                       >  1:1278645/1‑150 (MQ=255)
             atatcgctcTTGGGCGGTCTGTTCATTTCATCAATTGCATAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCa                                                                                                                                      <  8:868222/143‑1 (MQ=14)
              aTTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGGTTTGGCGGACTTTTCa                                                                                                                                      >  5:1148390/1‑150 (MQ=255)
               ttCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTCGTCTGTTCTGTGCAGCAATTTTCaa                                                                                                                                     >  3:265386/1‑150 (MQ=255)
                tCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTATGTTTTTTGTAGGACTTTTCCAAt                                                                                                                                   >  2:330820/1‑151 (MQ=17)
                tCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTGGTCAGTGCTTTGCAGCACGTATCatat                                                                                                                                   >  1:379464/1‑151 (MQ=17)
                  ccgcggggggggTCTGTACATTACAGCACTATCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAAttt                                                                                                                                 <  5:35163/145‑1 (MQ=17)
                    ccgggggTGCTCTGGGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTg                                                                                                                               <  3:1022460/147‑1 (MQ=255)
                       tgctttgtcttttcattACATCTCTTTCAAATTTTGCAGCTGTAACCTAGTCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGa                                                                                                                             <  2:897037/135‑1 (MQ=17)
                        ggtgggtCTGTGCCTTTAAGGACTTTAATATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGc                                                                                                                           <  7:1161273/147‑1 (MQ=17)
                        ggtgggtCTGTGCCTTACATCCCTATCAAAATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGc                                                                                                                           <  7:553592/147‑1 (MQ=255)
                         ggtggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGGAGCACTTTTCTAATTTTGGAGCg                                                                                                                          >  1:1037388/1‑151 (MQ=255)
                          gtggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGACTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCgg                                                                                                                         >  7:319537/1‑151 (MQ=255)
                           tggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGAGGTCTGTGCTGTGCAGGACTTTTCGCATTTAGGAGCggg                                                                                                                        >  4:976887/1‑151 (MQ=17)
                            ggtCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGAGGTATGTTGGTTGCAGCACTTTTCAAATTTATGAGAggg                                                                                                                        >  3:947007/1‑150 (MQ=17)
                              tCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGGACGTCTGTGCTGTGCAGGACTTTTCAAATTTAGGAGGCGGaa                                                                                                                      >  2:345607/1‑150 (MQ=17)
                               cTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGTTCGTATGTTCTGTGAAGGACTTTTCAAATTTAGGAGCGGGaacc                                                                                                                    >  2:368909/1‑149 (MQ=17)
                               cTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTAGGTGGTCTGTTATTTGCAGAAATTTTCAAATTTTTGAGGGGGaacc                                                                                                                    >  2:808046/1‑149 (MQ=17)
                                 gggcatttaagtaCTTTAAAATTTTGGATCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTAGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTTTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGaan                                                                                                                     <  1:993216/136‑2 (MQ=14)
                                  tgCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCCTTGGTCTGTGGTTTTCAGCACTTTTCAAATTTAGGAGAGGGAAACg                                                                                                                   >  6:445522/1‑149 (MQ=17)
                                   gCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACgagc                                                                                                                >  3:1067691/1‑150 (MQ=255)
                                    ccTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGAAGCACTTTTCAAATTTTGGAGAGGGAAACGAGAc                                                                                                               >  8:326483/1‑151 (MQ=255)
                                     cTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACGTCTTTGAGATTAAGCACTTTTCAAATTTAGGAGCGGGAAACgaaccc                                                                                                              >  2:130309/1‑147 (MQ=14)
                                         cAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGCTTTGGCGCACTTTTCAAATTTTGGAGGGGGAACCGGGCCTCGa                                                                                                           >  5:267245/1‑150 (MQ=17)
                                            cACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGGTTGCAGCAATTTTCAAATTTTGGAGTGGGAAACGAGCCTCGAACTc                                                                                                       >  1:353266/1‑151 (MQ=255)
                                                  cAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTGTGGAGGACTTTTCAAATTTTGGAGCGGGTACCGAGCGTGGATGTCGCGAcc                                                                                                 >  3:554068/1‑151 (MQ=14)
                                                  cAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGCGTGGTCTGTGGTATGAAGAAATTTTCAAATTTTGGAGTGGGTAACGGTTCTCGAAGTCGCGagc                                                                                                 >  4:1181831/1‑149 (MQ=14)
                                                    aaTTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTAAGTTATATGAAGAAATTTTCAAATTTATGAGACGGAACCGACCGTCGAACTAGCGAcaac                                                                                               >  6:1065532/1‑148 (MQ=11)
                                                      ttttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGCAGCACTTTTCAAATTTTTGAGCGGGAAACGGGACGCGAACTAGCGACCCCg                                                                                              >  7:160/1‑150 (MQ=255)
                                                         tGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCCGTGGTCTGTGCTATGCAGCACTTTTCAAATTTTGGAGCGGAAAACGAGACTCGAACTACCGACCACGAACt                                                                                          >  6:796444/1‑151 (MQ=14)
                                                           gAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTACGAGGTCTGTTATGTGGAGGAATTTTCAAATTTATGAGACGGAAACGCACCACGAACACGCGaacacgcacttg                                                                                        >  6:966831/1‑140 (MQ=2)
                                                            aGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGAAGGACTTTTCAAATTTTTGAGCGGGAAACGAGCCTCGAACTCGCGACCCAGACCTTgg                                                                                       >  8:1014123/1‑151 (MQ=255)
                                                              cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTATTTGAAGCAATTTTCAAATTTTGGAGCGGTAAACGAGCCTCGAAATCTCGACCCCGACCTTGGc                                                                                      >  8:723938/1‑150 (MQ=14)
                                                               gggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTGATTTGCAGCACTTTTCTAATTTTTGAGCGGGAAACGACCCGCGAAGTCGCGACACAGACCTTGGCa                                                                                     >  5:1119038/1‑150 (MQ=14)
                                                                ggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGGTGTTCTGTGATTTGCAGCAATTTTCAAATTTTGGAGAGGGAAAGGAGCCGCGAAATCGCGACCCCGACCTTggga                                                                                     >  4:673050/1‑147 (MQ=14)
                                                                   aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCCGGACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGCACTTGGCAAGGTc                                                                                >  8:197746/1‑151 (MQ=255)
                                                                   aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGGTTTGCCGGACTTTTCAAATTTTGGAGCGGGAACCGAGCCTCGATGTCGCGACCCCGCCCTTGCCAAggac                                                                                >  1:569934/1‑149 (MQ=14)
                                                                   aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGCGTTTCAGCACTTTTCAAATTTTTGAGAGGGAAACGAGACTCCAACTCGCGACCCCGACCTTCGCAAgcac                                                                                >  2:617594/1‑148 (MQ=11)
                                                                    aCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAACCGAGCCTCGAACTCGCGACCCCGCCCTTGGCAAGGTCg                                                                               >  1:986032/1‑151 (MQ=255)
                                                                      gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGTTGGTATGTGGTTTGGAGGACTTTTCGAATTTTGGAGGGGGTAACGAGACTCGATCTCGCGACCCAGACCTGCGCATGCTCGTg                                                                             >  3:839503/1‑151 (MQ=14)
                                                                      gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGCTTTGCAGGACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                                                             >  1:457207/1‑151 (MQ=255)
                                                                      gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCACGCTTGGGTGGTCTGTTATTTGGAGAACTTTTCAAATTTATGAGAGGGAACCGAAAGTCGAAGTAGCGACAACGACCTTGGGAAGGACGtc                                                                             >  6:883373/1‑150 (MQ=14)
                                                                        gaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGGAGCACTTTTCAAATTTTGGAGCGGGAAACGCGACTCGAACTCGCGACCCCGACCTTGGGAAGGTCGTGct                                                                           >  7:546026/1‑151 (MQ=255)
                                                                         aCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTCTGTGATTTGAAGAACTTTTCAAATTTTGGAGAGGGAAACGAGACTCGAAATCGCGACCCCTACCTTGGAAAgcaagacct                                                                           >  6:422188/1‑142 (MQ=14)
                                                                              aaCTCGCGACCCCGACCTTGGCAACGTAGTGCTCTACCAACTCAGCTATTCACTAATTCGAGGTCTGTGATTAGCAGCACTTTTCAAAATTTGTAGATGTAAACGAGCCTCGAAATAGCGACCACTACATTGGAAAgcaagacctctaac                                                                      >  8:547749/1‑137 (MQ=9)
                                                                               aCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGGTTTGGAGCACTTTTCAAATTTTGGAGCGGGTAACGAGCCTCGAACTCGCGACCCCGACCTTGGCATGGTCGTGCTCTACCa                                                                     >  3:715185/1‑150 (MQ=255)
                                                                               aCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTACCa                                                                     >  4:532577/1‑150 (MQ=38)
                                                                               aCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTATGTTGTTTTGAGCACTTTTCTAATTTTTGAGGGGGAAACGAGACTCGATCTAGCGACCCCTACGTTGGCAAGGTCGTCCTCTACCa                                                                     >  8:1113198/1‑150 (MQ=11)
                                                                                 tCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCTTGGTCTGTGATATTAAGCAATTTTCAAATTTTGGAGACGGAAACGAAACTCCAACTCACGACCAAGCACTAGGCAAGCTCCTGCTCAACAAACt                                                                  >  2:308803/1‑151 (MQ=2)
                                                                                   gcgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTTCAGAAATTTTCAAATTTTTGAGAGGGAAACGAGACTCGAAATCGCGACCCCGACATTGGAAAGGTCGTGCACAAACAACTg                                                                 >  4:610350/1‑150 (MQ=11)
                                                                                    cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAACCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTg                                                                 >  5:384383/1‑149 (MQ=38)
                                                                                    cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTCGGTGGTCTGTGCTTTGCAGGACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTAGCCAAGCTCGAGCTCTACCAACTGac                                                               >  2:10377/1‑150 (MQ=14)
                                                                                      aCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGAGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGGAAGCTCGTGCTCTACCAACTGAGCt                                                             >  8:259814/1‑151 (MQ=255)
                                                                                       ccccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGAAGCACTTTTCAAATTTTGGAGGGGGAAACGCGGCGCGAACTCGCGACCCCGACGTTGGCAAGCTCGTGCTCTACAAACTGAGCt                                                             >  5:5671/1‑150 (MQ=14)
                                                                                       ccccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGATTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCt                                                             >  2:1031573/1‑150 (MQ=38)
                                                                                        cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGAAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTAt                                                           >  6:285097/1‑151 (MQ=38)
                                                                                        cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTCGTCTGTGAGTTGAAGCACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCCCGACCACGACCTACGCAAGGTCGAGCTCTACCAACTGAGCTAt                                                           >  5:728031/1‑151 (MQ=11)
                                                                                         ccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTTTGGTTTGGAGCACTTTTCTAATTTTGGAGGGGGAAAGGGGCCTGGATCTCTCGACCCAGAGCTTCGGATGGTCGTGCTCTACAAACTGAGCTAtt                                                          >  5:1164929/1‑151 (MQ=14)
                                                                                           gACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTTCTGTGGAGGACTTTTCAAATTTTGGAGCGGGAAACGCGAGTCGAACTCGCGACCCCGCGGTTGGGAAGGTCGTGCGCTACAAACTGAGCTATTcg                                                        >  1:1108001/1‑150 (MQ=14)
                                                                                           gACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTCGTCTGTGATTTGAAGAAATTTTCAAATTTTTGAGAGGGAAACGAGCCTCGAACTCGCGACCCCGAACTTGGGAAGGTCGTGCTCTAACAACTGAGCTATTcc                                                        >  2:950075/1‑151 (MQ=14)
                                                                                           gACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGCGTGTTCTGTGGTTTTGAGGACTTTTCAAATTTTGGAGCGGGAAACGAGCCTCGAACTCGCGACACCGAGCTTGGCAAGGTCGTGCGCTACAAACTGAGGTATTcc                                                        >  1:795542/1‑151 (MQ=11)
                                                                                                tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTGATTTGGAGGACTTTTCACATTTGTGAGACGGAACCGCGCCACGATCTAGCGACCCAGCGGTAGGGAAGCTACTCCACTACAAACTGAGGTATTccctagt                                                   >  4:1157776/1‑147 (MQ=9)
                                                                                                   cAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTGGGTGGTCTGTGCTTTTCAGCAATTTTCAAATTTTGGAGAGGGAAACGAGACTCGAACTCGCGACCCCGACCTAGCCAAGGTCGTGCTCAACCAACTTAGCTATTccctctt                                                   >  5:886956/1‑144 (MQ=11)
                                                                                                        tCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTATGTTATTTGAAGAACTTTTCAAATTTTGGAGACGGAAACGCGAGTCGAACTCGCGACACCGAGCTTCGCAAGGTCGTGCTCTAAAAACTGAGATATTCCCGAGTACTACtt                                            >  4:382838/1‑150 (MQ=14)
                                                                                                         cGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTa                                           >  5:147359/1‑150 (MQ=255)
                                                                                                          gTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGGACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACAAACTGAGCTATTCCCGCGTACTACTTAAt                                         >  3:69221/1‑151 (MQ=255)
                                                                                                              tctACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTg                                     >  8:151213/1‑151 (MQ=255)
                                                                                                                 aCCAACTGAGCTATTCCCGCTTGGGAGGTCAGTGGTATGAAGCAATTTTCAAATTTTGGAGCGGGAAACGAGACTCAAACTATCGACCAAGAACTTGGAAAGGTCGAGCTCTAAAAACTGAGCTATTCAAGAGTACAACTTAATTTTGCt                                   >  6:354846/1‑150 (MQ=9)
                                                                                                                  ccAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCttt                                 >  1:257726/1‑151 (MQ=255)
                                                                                                                      cTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGt                              >  8:808206/1‑150 (MQ=255)
                                                                                                                      cTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGt                              >  8:626808/1‑150 (MQ=255)
                                                                                                                         aGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAtt                          >  3:907978/1‑151 (MQ=255)
                                                                                                                          gCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTTCTTAATTTTGCTTTCGTAAttt                         >  1:90151/1‑151 (MQ=255)
                                                                                                                          gCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAttt                         >  3:1192586/1‑151 (MQ=255)
                                                                                                                           cTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGATCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAtttt                        >  5:214692/1‑151 (MQ=255)
                                                                                                                           cTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAAtttt                        >  3:44902/1‑151 (MQ=255)
                                                                                                                            tATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTc                       >  3:333661/1‑151 (MQ=255)
                                                                                                                             aTTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCg                      >  4:266349/1‑151 (MQ=255)
                                                                                                                              ttCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     >  6:558695/1‑151 (MQ=255)
                                                                                                                               tCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGc                     >  3:34652/1‑150 (MQ=255)
                                                                                                                                   gCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTc                >  4:754525/1‑151 (MQ=255)
                                                                                                                                   gCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCGAGTCCCGCGTCCTTCTTAATTTTGCTTTCGTAATTTTCGGATTTc                >  8:663237/1‑151 (MQ=255)
                                                                                                                                   gCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCGACCAACTGAGCTATTCCCTCGTACTACTTAATTTTTCTTTCGTAATTTTCGCAttt                 >  2:488990/1‑150 (MQ=255)
                                                                                                                                     ttGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTg              >  4:1033023/1‑151 (MQ=255)
                                                                                                                                      tgtgtggtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTg              <  4:422294/147‑1 (MQ=255)
                                                                                                                                          tggtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtcg         >  5:847220/1‑151 (MQ=255)
                                                                                                                                           ggtGTGTGCTTTCGAGAATTTTTAAAATTTTGGAGCGGTAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtcg         <  7:851697/150‑1 (MQ=21)
                                                                                                                                           ggtCTGTGGTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGGAATTTTCGCATTTCTGcgtcgt        >  4:1098543/1‑151 (MQ=255)
                                                                                                                                           ggtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGcgtcgt        >  7:1223747/1‑151 (MQ=255)
                                                                                                                                            gtCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGtt       >  4:810553/1‑151 (MQ=255)
                                                                                                                                             tCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGtt       >  3:413734/1‑150 (MQ=255)
                                                                                                                                              cTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTAc     >  1:725031/1‑151 (MQ=255)
                                                                                                                                                gtgCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACgg   >  8:1267594/1‑151 (MQ=255)
                                                                                                                                                 tgCCTTACAGCAC‑TTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGAAATTTTCGAATTTATGCGACGTTACggg  >  4:727231/1‑150 (MQ=17)
                                                                                                                                                   cTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACggg  >  5:518171/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCCTTACAGCACTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCTTGGGTGGTCTGTGCTTTGCAGCACTTTTCAAATTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACTACTTAATTTTGCTTTCGTAATTTTCGCATTTCTGCGTCGTTACGGG  >  DH1/4121977‑4122272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑