BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	DH1	635,638	A→C	F118L (TTT→TTG)	yqjA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	DH1	635,638	0	A	C	97.3%	1278.2 / 8.6	337	F118L (TTT→TTG)	yqjA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (7/2); new base C (175/152); total (182/155)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 349 total aligned reads displayed.

AGGATCAATACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGAAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCGTTTGC  >  DH1/635489‑635785

 ggATCAATACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGcaa                                                                                                                                                   <  4:964745/151‑1 (MQ=255)

   atgaaTACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGGGTTCTGACAAACGCAATAAAGCGACCAATTAAAAGCGCCGATAAACCGTGTTTATGgaaa                                                                                                                                                  >  6:834329/4‑150 (MQ=255)

   aTCAATACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGcaaaa                                                                                                                                                 <  8:208564/151‑1 (MQ=255)

    tCAATACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGcaaa                                                                                                                                                  >  7:832257/1‑149 (MQ=255)

     cAATACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGa                                                                                                                                               <  8:1290406/151‑1 (MQ=255)

      aaTACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGAt                                                                                                                                              <  1:337408/151‑1 (MQ=255)

            cacaGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGtgtg                                                                                                                                        <  7:182709/151‑1 (MQ=255)

            cacaGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGGGTTTATGCAACAGATGGtgtg                                                                                                                                        >  2:1271711/1‑151 (MQ=255)

             acaGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTgc                                                                                                                                       >  5:566352/1‑151 (MQ=255)

             acaGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTgc                                                                                                                                       <  2:896672/151‑1 (MQ=255)

               agcagACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGCCCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTgagc                                                                                                                                     >  3:99946/1‑151 (MQ=255)

                  agACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGtt                                                                                                                                  <  7:861175/151‑1 (MQ=255)

                        cTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTgatgat                                                                                                                            >  3:475542/1‑151 (MQ=255)

                         tCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTgatgat                                                                                                                            >  1:966785/1‑150 (MQ=255)

                          cATCCAGTTGAAACACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATaa                                                                                                                          <  8:743651/151‑1 (MQ=255)

                          cATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATa                                                                                                                           >  2:332384/1‑150 (MQ=255)

                           aTCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAAt                                                                                                                         >  3:635539/1‑151 (MQ=255)

                           aTCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGGTGATGATAAt                                                                                                                         >  5:329384/1‑151 (MQ=255)

                           aTCAAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCAGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCTACCAATTAAAAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAAt                                                                                                                         <  1:119756/151‑1 (MQ=255)

                             ccAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGCCCAATTAACAGCCCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGGTGATGATAATgc                                                                                                                       >  6:879377/1‑151 (MQ=255)

                             ccAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATgg                                                                                                                       >  6:1139870/1‑150 (MQ=255)

                             ccAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATgc                                                                                                                       <  3:150664/151‑1 (MQ=255)

                             ccAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATg                                                                                                                        <  1:536711/150‑1 (MQ=255)

                             ccAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGAAAAAGATGGTGTGCGAGTTGATGATAATgc                                                                                                                       >  4:1145862/1‑151 (MQ=255)

                              cAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCGTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGGCCAATTAACAGCGCCGATTAACCGTGTTTATGAAAATGATGGTGTGCGCGTTGATGATAATgcg                                                                                                                      >  2:1106180/1‑151 (MQ=255)

                              cAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATgcg                                                                                                                      <  1:763323/151‑1 (MQ=255)

                                gTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCGGGCAATCGGCGGCAGCAGTGTTCTGACAAAGGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGcgcg                                                                                                                    <  2:215007/151‑1 (MQ=255)

                                gTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGcgcg                                                                                                                    <  8:775128/151‑1 (MQ=255)

                                   gAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCggg                                                                                                                  <  3:352896/150‑1 (MQ=255)

                                     aaaaCTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTaa                                                                                                               <  3:1172820/151‑1 (MQ=255)

                                     aaaaCTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTa                                                                                                                >  6:144328/1‑150 (MQ=255)

                                           gAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATga                                                                                                           <  1:702444/149‑1 (MQ=255)

                                              aGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGAt                                                                                                        >  2:1037081/1‑149 (MQ=255)

                                              aGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGAt                                                                                                        >  3:1088612/1‑149 (MQ=255)

                                              aGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAg                                                                                                      <  2:119126/151‑1 (MQ=255)

                                               gcgcgcGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGc                                                                                                     >  4:337229/1‑151 (MQ=255)

                                                  cgcgTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAg                                                                                                  <  6:504932/151‑1 (MQ=255)

                                                   gcgTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGt                                                                                                 <  1:168082/151‑1 (MQ=255)

                                                     gTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGttt                                                                                               <  6:1221512/151‑1 (MQ=255)

                                                       tATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGCCAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCTAAAGATGGTGTGCGCGGTGATGCGAATGCGCGGGTAAATGAGATAGCCAGGTTTg                                                                                             >  2:72247/1‑151 (MQ=255)

                                                       tATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACTAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTg                                                                                             >  7:1062221/1‑151 (MQ=255)

                                                       tATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGtttt                                                                                              <  7:998354/150‑1 (MQ=255)

                                                            aGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACgg                                                                                        <  3:673670/151‑1 (MQ=255)

                                                             gCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGt                                                                                       <  7:1010289/151‑1 (MQ=255)

                                                               ccTGATAAACCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAATGCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGGGCGGGTAAATGAGATAGCCAGGTTAGTACGGTGc                                                                                     >  6:596832/1‑151 (MQ=255)

                                                                     aaCCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTa                                                                                <  6:365962/150‑1 (MQ=255)

                                                                     aaCCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGCTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTAt                                                                               >  3:232934/1‑151 (MQ=255)

                                                                      aCCCGGCAATCGGCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTAtt                                                                              <  8:1046300/151‑1 (MQ=255)

                                                                       cccGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTg                                                                             >  6:1110625/1‑151 (MQ=255)

                                                                              aTCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGAACAAATAAAAGCGCCGATAAACCGTGTTTATGAAAAAGATGGTGTGCGCGTTGATGATAATGACCGGGTAAATGAGATAGCCAGTTTAGTACGGTCCGGCTATTGCCCAGcc                                                                      >  4:161347/1‑151 (MQ=255)

                                                                                cgtcgGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAGTGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCAt                                                                    >  5:997164/1‑151 (MQ=255)

                                                                                cgtcgGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCAt                                                                    >  4:639287/1‑151 (MQ=255)

                                                                                cgtcgGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCAt                                                                    <  7:1221054/151‑1 (MQ=255)

                                                                                 gtcgGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATc                                                                   <  5:1237961/151‑1 (MQ=255)

                                                                                     gcagcaGTGTTCTGACAAACGCAATAAAGGGACCAATTAACACCGCCGATAAACCGTGTTTATGGAAAAGATGGTGTGCGCGTTTATGATAATGCGCGGGTAAATGAGCTAGCCAGTTTTGTAGGGTGCGGGTATTGCCGAGCCATCGccg                                                               <  8:352300/151‑2 (MQ=255)

                                                                                     gcagcaGTGTTCTGACAAACGCAATAAAGCGACCAATTATCAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGccc                                                               <  4:1184917/151‑1 (MQ=255)

                                                                                     gcagcaGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGccc                                                               >  4:374603/1‑151 (MQ=255)

                                                                                       agcagTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGAAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCt                                                             >  2:253087/1‑151 (MQ=255)

                                                                                        gcagTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTg                                                            <  7:317879/151‑1 (MQ=255)

                                                                                         cagTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTg                                                            >  3:836499/1‑150 (MQ=255)

                                                                                          agTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGaa                                                          <  4:515855/151‑1 (MQ=255)

                                                                                            tgtTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAat                                                         >  7:1161927/1‑150 (MQ=255)

                                                                                              ttCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAatat                                                       >  1:463235/1‑150 (MQ=255)

                                                                                              ttCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAatat                                                       >  7:644895/1‑150 (MQ=255)

                                                                                                 tGACAACCGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGGGGGTATTGCCCAGCCATCGCCCCTGAATATAGCt                                                   >  2:647181/1‑151 (MQ=255)

                                                                                                   aCAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTg                                                  >  3:511084/1‑150 (MQ=255)

                                                                                                   aCAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTg                                                  >  7:510001/1‑150 (MQ=255)

                                                                                                        cGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCcag                                            <  5:432646/151‑1 (MQ=255)

                                                                                                          cAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCcagca                                          <  4:422505/151‑1 (MQ=255)

                                                                                                          cAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCcagca                                          <  2:965194/151‑1 (MQ=255)

                                                                                                          cAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGAGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCcagc                                           >  1:570704/1‑150 (MQ=255)

                                                                                                           aaTAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGAAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATCGCTGACCcagc                                           >  5:886669/1‑149 (MQ=255)

                                                                                                            aTAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCagcagc                                        >  4:813692/1‑151 (MQ=255)

                                                                                                            aTAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCagcagc                                        >  1:883487/1‑151 (MQ=255)

                                                                                                                   gACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCt                                 >  5:17298/1‑151 (MQ=255)

                                                                                                                   gACCAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCt                                 <  5:112265/151‑1 (MQ=255)

                                                                                                                     ccAATTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCACAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTgg                               >  4:695821/1‑151 (MQ=255)

                                                                                                                        aTTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTggcgg                            >  5:788710/1‑151 (MQ=255)

                                                                                                                        aTTAACAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTggcgg                            >  1:399273/1‑151 (MQ=255)

                                                                                                                             cAGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACg                       >  4:897118/1‑151 (MQ=255)

                                                                                                                              aGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGCCCCAGCAGCCGAGGCTGGCGGCAACgg                      >  5:1186076/1‑151 (MQ=255)

                                                                                                                              aGCGCCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACgg                      >  1:1061943/1‑151 (MQ=255)

                                                                                                                               gcgcCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGt                     <  8:607474/151‑1 (MQ=255)

                                                                                                                                 gcCGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTca                   <  8:109572/151‑1 (MQ=255)

                                                                                                                                  ccGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTc                    >  1:1082287/1‑149 (MQ=255)

                                                                                                                                  ccGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTc                    >  2:83911/1‑149 (MQ=255)

                                                                                                                                   cGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTcagc                 >  3:938110/1‑151 (MQ=255)

                                                                                                                                   cGATAAACCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTcagc                 <  3:385323/151‑1 (MQ=255)

                                                                                                                                       aaaCCGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTcagcagc              >  6:408247/1‑150 (MQ=255)

                                                                                                                                         aCCGGGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTcagcagc              >  1:613029/1‑148 (MQ=255)

                                                                                                                                          ccGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGaa          <  8:870232/151‑1 (MQ=255)

                                                                                                                                           cGTGTTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCACCGGTCAGCAGCAGAAt         >  4:1022295/1‑151 (MQ=255)

                                                                                                                                            gtgtTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATc        <  5:510154/151‑1 (MQ=255)

                                                                                                                                            gtgtTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATc        >  2:587739/1‑151 (MQ=255)

                                                                                                                                            gtgtTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATACCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATc        <  2:457201/151‑1 (MQ=255)

                                                                                                                                             tgtTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCg       >  5:626820/1‑151 (MQ=255)

                                                                                                                                              gtTTATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCGt      >  2:17910/1‑151 (MQ=255)

                                                                                                                                                ttATGCAAAAGATGGGGTGCGCGTTGATGATAATGCGCGGGTAAAGGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCGtt     <  6:226455/150‑1 (MQ=255)

                                                                                                                                                 tATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCGTTTg   >  5:659914/1‑151 (MQ=255)

                                                                                                                                                 tATGCAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCGTTTg   >  4:998257/1‑151 (MQ=255)

AGGATCAATACCCACAGCAGACCGCTCATCCAGTTGAAAAACTGAAAGCGCGCGTTATTCAGCCCTGATAACCCGGCAATCGTCGGCAGCAGTGTTCTGACAAACGCAATAAAGCGACCAATTAACAGCGCCGATAAACCGTGTTTATGAAAAAGATGGTGTGCGCGTTGATGATAATGCGCGGGTAAATGAGATAGCCAGTTTTGTACGGTGCGGGTATTGCCCAGCCATCGCCCCTGAATATAGCTGACCCAGCAGCCGAGGCTGGCGGCAACGGTCAGCAGCAGAATCGTTTGC  >  DH1/635489‑635785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`