Predicted mutation
evidence seq id position mutation annotation gene description
RA DH1 2,571,230 C→A R67S (CGC→AGC)  cls → cardiolipin synthase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,571,2300CA98.2% 1183.5 / 8.7 329R67S (CGC→AGC) clscardiolipin synthase A
Reads supporting (aligned to +/- strand):  ref base C (1/5);  new base A (163/160);  total (164/165)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 332 total aligned reads displayed.

GTTGCTCATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATCGCTGGGG  >  DH1/2571082‑2571377
                                                                                                                                                    |                                                                                                                                                   
   gCTCATTGCAGTCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCt                                                                                                                                                <  2:321352/151‑1 (MQ=255)
   gCTCATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAgcgc                                                                                                                                                 <  7:519365/150‑1 (MQ=255)
   gCTCATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTAGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCt                                                                                                                                                >  7:230517/1‑151 (MQ=255)
      cATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAgcgcag                                                                                                                                               <  7:1276149/149‑3 (MQ=255)
      cATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTg                                                                                                                                               >  5:492554/1‑149 (MQ=255)
      cATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTg                                                                                                                                               <  7:796075/149‑1 (MQ=255)
      cATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTAGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCtc                                                                                                                                               >  2:821035/1‑148 (MQ=255)
       aTTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCCGCGCTGAgc                                                                                                                                            <  6:780802/151‑1 (MQ=255)
         tGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAgcgc                                                                                                                                          >  3:224145/1‑151 (MQ=255)
         tGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAgcg                                                                                                                                           <  1:135027/150‑1 (MQ=255)
          gCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAgcgc                                                                                                                                          <  3:1271273/150‑1 (MQ=255)
            aGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTTCATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGGTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGcc                                                                                                                                       <  3:525161/151‑1 (MQ=255)
              gCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTAGATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCag                                                                                                                                     <  1:438695/151‑1 (MQ=255)
              gCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGcc                                                                                                                                       >  1:660770/1‑149 (MQ=255)
                  aaCTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCg                                                                                                                                 <  1:21025/151‑1 (MQ=255)
                    cTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGAt                                                                                                                               >  3:462967/1‑151 (MQ=255)
                     tttACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGAtg                                                                                                                              >  1:857405/1‑151 (MQ=255)
                     tttACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGAtg                                                                                                                              >  5:1049448/1‑151 (MQ=255)
                      ttACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAACCGCAGCGCTGAGCGCGCCAGAGCGAtgt                                                                                                                             >  4:876091/1‑151 (MQ=255)
                        aCGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTgg                                                                                                                           <  5:1184049/151‑1 (MQ=255)
                             ttCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTc                                                                                                                      >  8:1297937/1‑151 (MQ=255)
                                 aaTGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCa                                                                                                                    <  7:1029615/149‑1 (MQ=255)
                                  aTGAAACCACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGACGTGGCCTTCCACCg                                                                                                                 <  2:120504/151‑1 (MQ=255)
                                    gAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGc                                                                                                                >  8:743778/1‑150 (MQ=255)
                                     aaaCGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGGGCCAGAGCGATGTGGCCTTCCACCGCa                                                                                                               >  8:309015/1‑150 (MQ=255)
                                        cgacgCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAAt                                                                                                           <  3:163031/151‑1 (MQ=255)
                                        cgacgCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATGTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAAt                                                                                                           >  2:634673/1‑151 (MQ=255)
                                          acgCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATgg                                                                                                         <  7:540976/151‑1 (MQ=255)
                                           cgctCAGTTGCCTCCGCGATGGCCTGTCTGTTGTTTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGc                                                                                                        <  4:598502/151‑1 (MQ=255)
                                             cgcAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCtt                                                                                                      <  1:1123839/151‑1 (MQ=255)
                                             cgcAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCtt                                                                                                      >  6:574503/1‑151 (MQ=255)
                                             cgcAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTAATGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCtt                                                                                                      <  6:962338/151‑1 (MQ=255)
                                              gcAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCtt                                                                                                      >  5:357853/1‑150 (MQ=255)
                                              gcAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCtt                                                                                                      >  2:1166361/1‑150 (MQ=255)
                                              gcAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCATTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCtt                                                                                                      >  8:824343/1‑150 (MQ=255)
                                               cAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTaa                                                                                                    <  8:1259870/151‑1 (MQ=255)
                                                aGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAAc                                                                                                   >  5:632601/1‑151 (MQ=255)
                                                         cgcgATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTaaa                                                                                          <  4:436275/151‑1 (MQ=255)
                                                          gcgATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTaaac                                                                                         >  8:1032282/1‑150 (MQ=255)
                                                          gcgATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAg                                                                                         <  6:649208/151‑1 (MQ=255)
                                                          gcgATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAg                                                                                         <  5:167415/151‑1 (MQ=255)
                                                             aTGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGCGCGCGCCAGCGCGATGTGGCCTTCCACCGCAAAATGGCTTACCGACCTGAAAGCCt                                                                                      >  2:906812/1‑151 (MQ=255)
                                                             aTGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCt                                                                                      >  3:1174868/1‑151 (MQ=255)
                                                               ggCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTACGCAAACGCAGCGCTGCGCGCGCCAGAGCGATGAGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGt                                                                                    >  6:426016/1‑151 (MQ=255)
                                                                      cTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCat                                                                              >  4:180186/1‑150 (MQ=255)
                                                                      cTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCat                                                                              >  8:894178/1‑150 (MQ=255)
                                                                       gggtGACTAATTACCATCTGCCGGTACCCGGAAATATTGCCTATCGTGCCCGTGGCGAGCCCCAATTACGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCatat                                                                            <  2:25984/148‑1 (MQ=255)
                                                                        gTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCatat                                                                            <  3:793212/150‑1 (MQ=255)
                                                                        gTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATc                                                                           <  6:353241/151‑1 (MQ=255)
                                                                          tGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCtt                                                                         >  7:492151/1‑151 (MQ=255)
                                                                           gATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTc                                                                        <  7:766788/151‑1 (MQ=255)
                                                                           gATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTc                                                                        <  1:228035/151‑1 (MQ=255)
                                                                            attattTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCg                                                                       <  7:108756/151‑1 (MQ=255)
                                                                            attattTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCg                                                                       >  4:1157182/1‑151 (MQ=255)
                                                                             ttattTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGc                                                                      >  2:829464/1‑151 (MQ=255)
                                                                              tattTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGc                                                                      <  2:525887/150‑1 (MQ=255)
                                                                               attTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCg                                                                    >  3:945850/1‑151 (MQ=255)
                                                                                   aCATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCga                                                                   >  6:880688/1‑148 (MQ=255)
                                                                                    cATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCgaaga                                                                >  5:734679/1‑150 (MQ=255)
                                                                                     aTTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCgaagaa                                                               >  3:66716/1‑150 (MQ=255)
                                                                                     aTTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCgaagaa                                                               >  4:404113/1‑150 (MQ=255)
                                                                                         tGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAAt                                                            >  5:404837/1‑149 (MQ=255)
                                                                                          gCCGTTAGTCGGAATTATTGCCTATCTCGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAAt                                                            <  1:1012287/148‑1 (MQ=255)
                                                                                           ccGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGc                                                         >  8:255714/1‑150 (MQ=255)
                                                                                            cGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGc                                                         >  7:726231/1‑149 (MQ=255)
                                                                                            cGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGc                                                         >  2:1261720/1‑149 (MQ=255)
                                                                                             ggtAGGCGGAAGTATTGCCTCTCTTGCCGGTGGCGAGCTCCATTTACGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTACGCATATCTTCGCCGAAGAAAATAGCAgt                                                      <  6:1290765/149‑1 (MQ=255)
                                                                                               tAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAgtgt                                                    >  4:642766/1‑151 (MQ=255)
                                                                                               tAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAgtg                                                     <  1:1166685/150‑1 (MQ=255)
                                                                                                    ggAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTg                                               <  7:401169/151‑1 (MQ=255)
                                                                                                          attGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCa                                          <  5:108382/150‑1 (MQ=255)
                                                                                                           ttGCCTATCTTGCCGTTGGCGAGCTGCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCGGAAGAAAATAGCAGTGTCGCTGCGCCatt                                        >  8:579440/1‑151 (MQ=255)
                                                                                                             gCCTCTCTTGCCGTTGGCGAGCTGCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAATATAGCAGTGTCGCTGCGCCatta                                       <  1:495915/150‑1 (MQ=255)
                                                                                                             gCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCattat                                      >  1:584675/1‑151 (MQ=255)
                                                                                                             gCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCatta                                       <  4:124743/150‑1 (MQ=255)
                                                                                                             gCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCatta                                       <  2:394536/150‑1 (MQ=255)
                                                                                                              ggtATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGGCAGTGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCattatt                                     <  3:1106383/149‑1 (MQ=255)
                                                                                                               cTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTc                                    >  8:682081/1‑151 (MQ=255)
                                                                                                                tATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCTCCATTATTc                                    >  4:705266/1‑150 (MQ=255)
                                                                                                                tATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTc                                    >  5:923363/1‑150 (MQ=255)
                                                                                                                 aTCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCa                                   >  2:202590/1‑150 (MQ=255)
                                                                                                                  tCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCa                                   >  2:1146327/1‑149 (MQ=255)
                                                                                                                    ttGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCt                               >  3:964459/1‑151 (MQ=255)
                                                                                                                     tGCCGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCt                               <  6:272946/150‑1 (MQ=255)
                                                                                                                        cGTTGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGc                           >  5:890954/1‑151 (MQ=255)
                                                                                                                           tGGCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCg                          >  7:284664/1‑149 (MQ=255)
                                                                                                                            ggCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGc                       <  7:1174181/151‑1 (MQ=255)
                                                                                                                            ggCGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGc                       <  8:346201/151‑1 (MQ=255)
                                                                                                                              cGAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGcgt                     >  6:1058964/1‑151 (MQ=255)
                                                                                                                               gAGCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCGATTATTCAAGCTTTGCGAGcgtc                    >  4:345918/1‑151 (MQ=255)
                                                                                                                                 gCTCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGcgtcgt                  >  1:361163/1‑151 (MQ=255)
                                                                                                                                   tCCATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCgtcgtc                 >  4:266082/1‑150 (MQ=255)
                                                                                                                                     cATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAgg              <  2:143976/151‑1 (MQ=255)
                                                                                                                                     cATTTAGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAgg              >  5:1012438/1‑151 (MQ=255)
                                                                                                                                          aGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATc         <  5:238269/151‑1 (MQ=255)
                                                                                                                                          aGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATc         >  2:750986/1‑151 (MQ=255)
                                                                                                                                          aGGCAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATc         <  1:830770/151‑1 (MQ=255)
                                                                                                                                             cAAACGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATCGCt      <  8:872531/151‑1 (MQ=255)
                                                                                                                                              aaaCGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATCGCTg     >  2:1079211/1‑151 (MQ=255)
                                                                                                                                               aaCGCAGCGCTGAGCGCGCCAGAGCGATGTGGCCATCCACCGCAAAATGCCTAAACGACCTAAAAGCCTGTAAGCATATCATCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATCGCTgg    <  6:1015996/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTTGCTCATTGCAGGCGTAACTTTACGCATTCTAATGAAACGACGCGCAGTTCCCTCCGCGATGGCCTGGCTGTTGATTATTTACATTCTGCCGTTAGTCGGAATTATTGCCTATCTTGCCGTTGGCGAGCTCCATTTAGGCAAACGCCGCGCTGAGCGCGCCAGAGCGATGTGGCCTTCCACCGCAAAATGGCTTAACGACCTTAAAGCCTGTAAGCATATCTTCGCCGAAGAAAATAGCAGTGTCGCTGCGCCATTATTCAAGCTTTGCGAGCGTCGTCAGGGGATCGCTGGGG  >  DH1/2571082‑2571377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑