| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | DH1 | 3,668,924 | (T)5→6 | pseudogene (353/455 nt) | lpxA ← | hydroxymyristoyl‑ACP dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | DH1 | 3,668,919 | 1 | . | T | 97.6% | 1485.6 / 20.2 | 412 | pseudogene (358/455 nt) | lpxA | hydroxymyristoyl‑ACP dehydratase |
| Reads supporting (aligned to +/- strand): ref base . (8/2); new base T (196/204); total (206/206) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 439 total aligned reads displayed.
CCCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑T‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT > DH1/3668769‑3669064 | cccTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGcgc‑g > 8:1072961/1‑151 (MQ=255)cccTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGcgc‑g > 7:1079673/1‑151 (MQ=255) ccTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑t > 2:1750693/1‑151 (MQ=255) tctTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑t‑tt > 6:1508108/1‑151 (MQ=255) tCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑t‑tt < 5:343601/148‑1 (MQ=255) ggCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGt < 7:1193624/150‑1 (MQ=255) ggCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTg < 4:1010253/151‑1 (MQ=255) ggCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTg < 5:1445731/151‑1 (MQ=255) gCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTg > 1:1356750/1‑150 (MQ=255) gCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGa < 1:1218035/151‑1 (MQ=255) gCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGa < 4:1523760/151‑1 (MQ=255) ggTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACt > 1:919356/1‑151 (MQ=255) ggTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACt < 1:1252966/151‑1 (MQ=255) gTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACtt > 3:1499568/1‑151 (MQ=255) tGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCACCCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑TGTT‑TT‑CGAAAGTGACTTcc > 2:663472/1‑151 (MQ=255) tGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTcc > 6:1873773/1‑151 (MQ=255) tGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTcc > 6:1645737/1‑151 (MQ=255) ggATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGAACACATCATCGTTGCTTCTCAAACTACTTTACCATCGACCAGAGCAACACCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTcc > 1:1674838/1‑150 (MQ=255) gCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGa < 3:837101/150‑1 (MQ=255) gCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGa < 5:564669/150‑1 (MQ=255) cacaAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATc < 1:469231/151‑1 (MQ=255) caAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCAt > 5:275902/1‑151 (MQ=255) aaaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCTCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGAGG‑G‑T‑TTGTT‑CGAAAGTGACTTCCATGATCAtt > 4:169049/1‑151 (MQ=255) aaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCAttt < 5:1524007/151‑1 (MQ=255) aaGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCAttt < 7:1658830/151‑1 (MQ=255) ggCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGa < 8:1722092/151‑1 (MQ=255) gCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGAt < 1:148457/151‑1 (MQ=255) ggATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCg < 7:1124139/151‑1 (MQ=255) gATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGAAGTCCATGATCATTTGATCGc > 7:395033/1‑151 (MQ=255) gATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑TGTT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGc > 8:379315/1‑151 (MQ=255) atcaatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGc < 8:958661/151‑1 (MQ=255) caatcaCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCAc < 6:1187996/151‑1 (MQ=255) caCGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGAc < 1:1444456/150‑1 (MQ=255) caCGTATCAGGCCTCCCGGCGACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTCACTTCCATGATCATTTGATCGCCAGGCACGAc < 2:1466604/150‑1 (MQ=255) cGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCgg > 4:1250204/1‑151 (MQ=255) gTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCggg > 4:106242/1‑151 (MQ=255) tATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgc > 5:112141/1‑151 (MQ=255) aTCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGgcg < 4:636094/151‑1 (MQ=255) cggCCTCCCGGATACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGTCTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCtt < 2:1643207/150‑1 (MQ=255) gCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTg > 2:831380/1‑150 (MQ=255) gCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTg > 6:780679/1‑150 (MQ=255) ccTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGaa < 3:1276462/151‑1 (MQ=255) cTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGa > 6:521312/1‑149 (MQ=255) tCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGCCTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgc > 2:1351162/1‑151 (MQ=255) cccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcg > 7:600093/1‑151 (MQ=255) ccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgc < 6:1338228/151‑1 (MQ=255) ccGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAgcgc > 7:236767/1‑151 (MQ=255) ggCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCTCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGACgcgcgc > 3:659313/1‑151 (MQ=255) gCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCt > 6:18027/1‑151 (MQ=255) aCGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCtt < 2:1231730/149‑1 (MQ=255) aCGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTc < 1:1010347/150‑1 (MQ=255) gAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTc < 3:561400/151‑1 (MQ=255) aGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCa < 6:375044/151‑1 (MQ=255) cacacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTGCGTCAAt < 7:1498887/151‑1 (MQ=255) cacaTCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATAc > 8:811107/1‑151 (MQ=255) catcatCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATAcc > 3:1828716/1‑150 (MQ=255) atcatcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATAcc > 2:1025402/1‑149 (MQ=255) catcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGc < 2:1879597/150‑1 (MQ=255) catcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCg < 6:235536/151‑1 (MQ=255) tcGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAAATGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCGTCGTCAATACCAGCGa > 4:1281050/1‑150 (MQ=255) cGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGa > 2:1107634/1‑149 (MQ=255) gCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAagtagt > 8:581847/1‑151 (MQ=255) tCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGCGG‑T‑TT‑TT‑GGAAACTGACTTACATGATCATTTGATCGCCAGGCACGCCCGGGCGCGTGAAGCGCGCGGAGGCAATACCACCGCAGTAGTAc > 7:1839399/1‑150 (MQ=255) tCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTAc < 6:887321/150‑1 (MQ=255) tCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTAc < 1:611961/150‑1 (MQ=255) cGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGGCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAg < 2:394320/151‑1 (MQ=255) cAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCt > 1:274799/1‑151 (MQ=255) aaaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCCTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTc < 5:1668219/151‑1 (MQ=255) aaCTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTcc > 1:307993/1‑150 (MQ=255) actactTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCAc < 1:1803908/151‑1 (MQ=255) tactTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCAccc > 2:950030/1‑151 (MQ=255) actTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCg > 5:209936/1‑151 (MQ=255) actTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑T‑TT‑TTCCGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCg < 2:194681/151‑1 (MQ=255) ttACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCggct > 8:1273746/1‑149 (MQ=255) ttACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCgg < 4:473444/149‑1 (MQ=255) tACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGCCCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTc > 3:415488/1‑151 (MQ=255) tACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGtt < 5:183013/150‑1 (MQ=255) aCCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAACTAGTACAGCTCACCCCGTTcc > 8:1442248/1‑151 (MQ=255) aTCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGt < 5:907322/151‑1 (MQ=255) aTCGACCAGAGCAACCACTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAACTAGTACAGCTCACCCGGTTCCAGt > 4:1602424/1‑151 (MQ=255) tCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGtt > 4:726390/1‑151 (MQ=255) gACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGtttt > 5:1286294/1‑151 (MQ=255) cAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCCCCCCGTTCCAGGTTTccg > 6:1700735/1‑150 (MQ=255) agagCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCt > 3:1053457/1‑150 (MQ=255) gCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGc < 3:1673116/151‑1 (MQ=255) gCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGc > 3:1390852/1‑151 (MQ=255) aaCCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtt > 4:371936/1‑151 (MQ=255) aaCCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCACTTTTCCTACGCtt > 1:723916/1‑151 (MQ=255) aCCCCTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCgtt > 3:1198538/1‑151 (MQ=255) ccccTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCtttt > 3:480704/1‑151 (MQ=255) ccTTTAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTa > 5:823589/1‑150 (MQ=255) ttAAAACGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTaaa < 6:657279/149‑1 (MQ=255) aaaaCGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTAACCCGGTTGCAGTTTTGCTACGCTTTTAAAAGcc > 2:587433/1‑151 (MQ=255) aaaCGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGcc > 7:848295/1‑150 (MQ=255) aaCGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGcc > 4:180516/1‑149 (MQ=255) aaCGGGTCAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTACTACGCTTTTAAACGcc > 8:68871/1‑149 (MQ=255) cAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGGACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCt < 4:980802/151‑1 (MQ=255) cAGGCCGCGGCGC‑GTT‑TT‑TT‑CGAAAGTGACATCCATGATCATTTGATCGCCAGGCAAGACCGGGCCCATGAAGCGCGCTTCGTCAAAACAATCGAAGTACAACACCTCACCCGGTTCCAGTTTTCCTACGCGTTTAAACGCCAGAATACCt > 3:281685/1‑151 (MQ=255) ggcgC‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCtg > 8:1794025/1‑151 (MQ=255) gcgc‑GTT‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCtgt < 3:372121/151‑1 (MQ=255) | CCCTCTTCCACAATGGCGGTTGGATGCACAAAGGCGGATTTATCAATCACGTATCAGGCCTCCCGGCTACGAGCACACATCATCGTTGCTTCGCAAACTACTTTACCATCGACCAGAGCAACCCCTTTAAAACGGGTCAGGCCGCGGCGC‑G‑T‑TT‑TT‑CGAAAGTGACTTCCATGATCATTTGATCGCCAGGCACGACCGGGCGCTTGAAGCGCGCTTCGTCAATACCAGCGAAGTAGTACAGCTCACCCGGTTCCAGTTTTCCTACGCTTTTAAACGCCAGAATACCTGTTGCCTGT > DH1/3668769‑3669064 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |