Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1294,9760TC53.1% 102.9 / inf 455S132P (TCG→CCG) ugpCglycerol‑3‑phosphate ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (124/87);  new base C (125/114);  total (250/205)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 470 total aligned reads displayed.

TTCCAGAACTACGCGCTTTATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCGTCTTGAACTGCAACAGTTGC  >  DH1/294826‑295123
                                                                                                                                                      |                                                                                                                                                   
ttCCAGAACTACGCGCTTTATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGtcgct                                                                                                                                                     >  8:1307593/1‑151 (MQ=255)
  ccAGAACTACGCGCTTTATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGtcgctc                                                                                                                                                    <  3:139522/150‑1 (MQ=255)
      aaCTACGCGCTTTATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTcgcgcg                                                                                                                                               >  5:1347608/1‑151 (MQ=255)
      aaCTACGCGCTTTATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCcgcgcg                                                                                                                                               >  4:1260246/1‑151 (MQ=255)
        caaCGCGCTTTATCCGCATATGAGTGTCGAAGAAACCATGGCGTGGGGCCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGATCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTcgcccg                                                                                                                                               >  5:39657/3‑149 (MQ=255)
         tACGCGCTTTATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGc                                                                                                                                            >  3:1796323/1‑151 (MQ=255)
            gcgcTTTATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCttt                                                                                                                                         >  7:222091/1‑151 (MQ=255)
            gcgcTTTATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCttt                                                                                                                                         >  4:581431/1‑151 (MQ=255)
              gcTTTATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTcc                                                                                                                                       <  2:1210278/151‑1 (MQ=255)
               cTTTATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCcg                                                                                                                                      >  5:197601/1‑151 (MQ=255)
                tttATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCcgg                                                                                                                                     >  4:884671/1‑151 (MQ=255)
                 ttATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCcg                                                                                                                                      <  8:1745540/149‑1 (MQ=255)
                   aTCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCcggcg                                                                                                                                   <  5:660388/150‑1 (MQ=255)
                   aTCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCcggcgg                                                                                                                                  >  8:883074/1‑151 (MQ=255)
                        aatatGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAgc                                                                                                                             <  8:1108447/150‑1 (MQ=255)
                           atGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGcc                                                                                                                          >  6:1586119/1‑151 (MQ=255)
                                 gtCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTCCCGAGCGCGTTAAAGAAGAGGCGCGCATTCTGGAGCTGGACGGTGTGCTCAAACGTCGCTCGCGCCAGCTTTCCGGCGGTCAGCGCCAGCgtg                                                                                                                    >  8:1905639/1‑151 (MQ=255)
                                   cGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGtgtg                                                                                                                  <  8:1590141/151‑1 (MQ=255)
                                     aagaaAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGc                                                                                                                >  2:1806235/1‑151 (MQ=255)
                                      agaaAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCg                                                                                                               <  5:1045909/151‑1 (MQ=255)
                                          aaCATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATgg                                                                                                           >  5:1534843/1‑151 (MQ=255)
                                           aCATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATggg                                                                                                          <  2:152574/151‑1 (MQ=255)
                                            cctGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGc                                                                                                         <  4:1692211/149‑1 (MQ=255)
                                            cATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGc                                                                                                         >  5:1153240/1‑151 (MQ=255)
                                             aTGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGcc                                                                                                        >  1:1289080/1‑151 (MQ=255)
                                                 cGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCcgcgc                                                                                                    <  3:1897523/151‑1 (MQ=255)
                                                 cGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCcgcgc                                                                                                    <  4:255721/151‑1 (MQ=255)
                                                 cGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCcgcgc                                                                                                    >  2:1703092/1‑151 (MQ=255)
                                                  gTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCcgcgcg                                                                                                   >  1:1776523/1‑151 (MQ=255)
                                                    gggggCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGAt                                                                                                 >  6:988894/1‑151 (MQ=255)
                                                          tGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTgcg                                                                                           >  7:800311/1‑151 (MQ=255)
                                                          tGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTgcg                                                                                           >  3:1049330/1‑151 (MQ=255)
                                                           gAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTgcgc                                                                                          >  7:1434629/1‑151 (MQ=255)
                                                           gAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTgcg                                                                                           <  1:220790/150‑1 (MQ=255)
                                                            aaaaTTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGcgcg                                                                                         >  4:1393704/1‑151 (MQ=255)
                                                              aaTTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGAt                                                                                       >  3:1731310/1‑151 (MQ=255)
                                                               aTTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATc                                                                                      >  3:1098897/1‑151 (MQ=255)
                                                                  cgcgGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCcgg                                                                                   >  3:352144/1‑151 (MQ=255)
                                                                  cgcgGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCcg                                                                                    <  1:404438/150‑1 (MQ=255)
                                                                   gcgGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGGCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCcggc                                                                                  <  5:979176/151‑1 (MQ=255)
                                                                    cgGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGCTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCcggcg                                                                                 >  1:1641210/1‑151 (MQ=255)
                                                                     ggCATGCGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGCGCCGCGCGATTGTGCGCGATCcggcgg                                                                                >  6:174831/1‑151 (MQ=255)
                                                                      gCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGgt                                                                               >  7:723167/1‑151 (MQ=255)
                                                                       cATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGgtg                                                                              >  2:726885/1‑151 (MQ=255)
                                                                          gggCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGtt                                                                            <  7:1527237/150‑1 (MQ=255)
                                                                            gCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCt                                                                         >  3:636768/1‑151 (MQ=255)
                                                                             cAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTg                                                                        <  7:469216/151‑1 (MQ=255)
                                                                             cAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTg                                                                        >  5:1419434/1‑151 (MQ=255)
                                                                             cAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGGCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTg                                                                        <  7:1271696/151‑1 (MQ=255)
                                                                               agcagcAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCAGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGtt                                                                      <  3:980752/151‑1 (MQ=255)
                                                                                    caAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTtgatg                                                                 >  6:166182/1‑151 (MQ=255)
                                                                                     aaaTTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTtgatg                                                                 >  2:909214/1‑150 (MQ=255)
                                                                                     aaaTTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTtgatg                                                                 >  8:1037516/1‑150 (MQ=255)
                                                                                          gCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCg                                                            <  6:1481361/150‑1 (MQ=255)
                                                                                             gAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGctct                                                        >  6:348290/1‑151 (MQ=255)
                                                                                              aGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGctctc                                                       <  8:1212823/151‑1 (MQ=255)
                                                                                              aGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGctctc                                                       >  7:1209919/1‑151 (MQ=255)
                                                                                               gcgcGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACTTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGctctct                                                      <  3:1300629/151‑1 (MQ=255)
                                                                                               gcgcGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGctctct                                                      <  3:444128/151‑1 (MQ=255)
                                                                                                   gTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGGTCCTGTTTGATGAGCCGCTCTCTAAcc                                                  <  5:815415/151‑1 (MQ=255)
                                                                                                    ttAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCt                                                 >  3:875419/1‑151 (MQ=255)
                                                                                                      aaaGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCg                                               >  4:539093/1‑151 (MQ=255)
                                                                                                        agaagCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGAt                                             <  6:623823/151‑1 (MQ=255)
                                                                                                         gaagCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCGGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATg                                            >  1:774541/1‑151 (MQ=255)
                                                                                                         gaagCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATg                                            >  6:1415444/1‑151 (MQ=255)
                                                                                                         gaagCGGCGCGCATTCTGGAGCTGGACGGGCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATg                                            <  8:551200/151‑1 (MQ=255)
                                                                                                            gcggcgCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGcc                                          >  8:304028/1‑150 (MQ=255)
                                                                                                               gcgcgcATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGc                                      >  8:813675/1‑151 (MQ=255)
                                                                                                               gcgcgcATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGc                                      <  3:1483153/151‑1 (MQ=255)
                                                                                                                  cgcATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTgc                                   >  7:160238/1‑151 (MQ=255)
                                                                                                                  cgcATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTgc                                   <  4:35273/151‑1 (MQ=255)
                                                                                                                   gcATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGTGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTgcg                                  <  6:1106222/151‑1 (MQ=255)
                                                                                                                   gcATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTgcg                                  >  3:1261695/1‑151 (MQ=255)
                                                                                                                   gcATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTgcg                                  >  1:1406194/1‑151 (MQ=255)
                                                                                                                    cATTCTGGAGCTGGACGGTCTCCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCAGCTCTCTAACCTCGATGCCAAGCTgcgc                                 >  2:78649/1‑151 (MQ=255)
                                                                                                                    cATTCTGGAGCGGGACGGGCTGCTCAAACGTCGCGCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTG‑TTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGcgcg                                <  6:879611/151‑1 (MQ=255)
                                                                                                                     aTTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCACCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGcgcg                                >  4:79006/1‑151 (MQ=255)
                                                                                                                     aTTCTGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGcgcg                                >  7:64820/1‑151 (MQ=255)
                                                                                                                       tCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGt                               <  8:1482351/150‑1 (MQ=255)
                                                                                                                        gtGGAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGc                             <  2:1861282/150‑1 (MQ=255)
                                                                                                                          ggAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGc                             <  2:1011689/149‑1 (MQ=255)
                                                                                                                          ggAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTg                              <  4:1301985/148‑1 (MQ=255)
                                                                                                                           gAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCGAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGc                             >  4:443503/1‑148 (MQ=255)
                                                                                                                           gAGCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGc                             >  6:489498/1‑148 (MQ=255)
                                                                                                                            cgCTGGACGGGCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGAt                         <  8:1415279/150‑1 (MQ=255)
                                                                                                                            aGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGc                             >  2:133779/1‑147 (MQ=255)
                                                                                                                            aGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGAt                         <  1:1143105/151‑1 (MQ=255)
                                                                                                                             gCTGGACGGTCTGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATg                        >  2:438578/1‑151 (MQ=255)
                                                                                                                                ggACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGc                       <  7:931274/149‑1 (MQ=255)
                                                                                                                                   cGGTCTGCTCAACCGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCGTCtt                  <  8:684692/151‑1 (MQ=255)
                                                                                                                                     gTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCGTCTTGa                <  2:1091465/151‑1 (MQ=255)
                                                                                                                                        tGCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCGTCTTGAACt             >  4:1509919/1‑151 (MQ=255)
                                                                                                                                         gCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCGTCTTGAACTg            >  3:1597543/1‑151 (MQ=255)
                                                                                                                                         gCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCGTCTTGAACt             <  5:1279931/150‑1 (MQ=255)
                                                                                                                                         gCTCAAACGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCGTCTTGAACt             <  6:1312341/150‑1 (MQ=255)
                                                                                                                                            cAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCGTCTTGAACTGCaa         <  1:1005782/151‑1 (MQ=255)
                                                                                                                                              aaCGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCGTCTTGAACTGCAACa       <  7:11026/151‑1 (MQ=255)
                                                                                                                                               aCGTCGCCCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCGTCTTGAACTGCAAc        >  2:263024/1‑149 (MQ=255)
                                                                                                                                                cGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCGTCTTGAACTGCAACAGt     >  7:1699618/1‑151 (MQ=255)
                                                                                                                                                 gTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCGGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCTTCTTGAACTGCAACAGtt    <  1:665624/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TTCCAGAACTACGCGCTTTATCCGCATATGAGTGTCGAAGAAAACATGGCGTGGGGGCTGAAAATTCGCGGCATGGGCAAGCAGCAAATTGCCGAGCGCGTTAAAGAAGCGGCGCGCATTCTGGAGCTGGACGGTCTGCTCAAACGTCGCTCGCGCGAGCTTTCCGGCGGTCAGCGCCAGCGTGTGGCGATGGGCCGCGCGATTGTGCGCGATCCGGCGGTGTTCCTGTTTGATGAGCCGCTCTCTAACCTCGATGCCAAGCTGCGCGTGCAGATGCGTCTTGAACTGCAACAGTTGC  >  DH1/294826‑295123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑