Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,542,2830CT53.0% 108.5 / inf 471G522S (GGC→AGC) acnAaconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base C (98/121);  new base T (111/136);  total (213/258)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

Only 100 of 477 total aligned reads displayed.

GCTCAGAAGCCAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGATAATCAGAAACGACTTTCGAACC  >  DH1/2542133‑2542433
                                                                                                                                                      |                                                                                                                                                      
 cTCAGAAGCCAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCt                                                                                                                                                       >  5:112771/1‑151 (MQ=255)
   cAGAAGCCAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCttt                                                                                                                                                     <  6:1352076/151‑1 (MQ=255)
   cAGAAGCCAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCtt                                                                                                                                                      <  6:30441/150‑1 (MQ=255)
    aGAAGCCAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTtttt                                                                                                                                                    <  7:804738/151‑1 (MQ=255)
    aGAAGCCAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTtttt                                                                                                                                                    <  8:145651/151‑1 (MQ=255)
     gAACCCAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGCATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCCCCGCACCGACGCTTAAATCGCtttttt                                                                                                                                                   >  6:149786/1‑151 (MQ=255)
      aaGCCAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTtttttt                                                                                                                                                  <  1:910921/151‑1 (MQ=255)
      aaGCCAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCtttttt                                                                                                                                                  <  2:1417193/151‑1 (MQ=255)
         ccAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGAt                                                                                                                                               <  3:1895462/151‑1 (MQ=255)
         ccAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGAt                                                                                                                                               >  2:235935/1‑151 (MQ=255)
          cAGGTTGTTATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGAtt                                                                                                                                              >  3:1349421/1‑151 (MQ=255)
          cAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGAtt                                                                                                                                              <  7:1511303/151‑1 (MQ=255)
          cAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGAtt                                                                                                                                              <  6:1201478/151‑1 (MQ=255)
           aGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTg                                                                                                                                             <  8:648185/151‑1 (MQ=255)
           aGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTg                                                                                                                                             >  1:198766/1‑151 (MQ=255)
              ttGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCg                                                                                                                                          >  1:1531811/1‑151 (MQ=255)
                gATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGtt                                                                                                                                        >  4:67043/1‑151 (MQ=255)
                   atTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCg                                                                                                                                     <  8:1169431/151‑1 (MQ=255)
                   atTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCg                                                                                                                                     <  8:848039/151‑1 (MQ=255)
                   atTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCg                                                                                                                                     <  1:498249/151‑1 (MQ=255)
                   atTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCg                                                                                                                                     >  6:1886720/1‑151 (MQ=255)
                   atTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCg                                                                                                                                     <  7:1129094/151‑1 (MQ=255)
                      cATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGAt                                                                                                                                   >  6:41683/1‑150 (MQ=255)
                         atTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAgg                                                                                                                                >  7:10260/1‑150 (MQ=255)
                           ttCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGAt                                                                                                                              <  7:1804704/150‑1 (MQ=255)
                           ttCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGGTTCGATAGGATc                                                                                                                             >  2:1041192/1‑151 (MQ=255)
                             cccGCCAGCGCATAGGCAACCAGCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGGTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCg                                                                                                                            <  2:985910/150‑1 (MQ=255)
                              ccGCCAGCGCATAGGCAACCACGAGCGGAGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGGAGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCggg                                                                                                                          <  7:1600421/151‑1 (MQ=255)
                              ccGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCggg                                                                                                                          >  7:450590/1‑151 (MQ=255)
                               cGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCCGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACCGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGc                                                                                                                         >  3:416386/1‑151 (MQ=255)
                                gCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGGTTTACCCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTGAACTCGCGGATTTTGATAGCCGTTTCGATAGGATCGGGc                                                                                                                         >  6:911336/1‑150 (MQ=255)
                                  cAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGc                                                                                                                      <  1:1415817/151‑1 (MQ=255)
                                  cAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGc                                                                                                                      >  8:243938/1‑151 (MQ=255)
                                    gcgcATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCgg                                                                                                                    >  8:1830218/1‑151 (MQ=255)
                                      gcATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGcc                                                                                                                  >  6:1794818/1‑151 (MQ=255)
                                       cATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGCCAGCACCGCACCGACGGTTAAATCGCGTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGccc                                                                                                                 >  6:767796/1‑151 (MQ=255)
                                            gCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGt                                                                                                            >  7:877774/1‑151 (MQ=255)
                                                 caccaGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCa                                                                                                       <  5:1604371/151‑1 (MQ=255)
                                                 caccaGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCa                                                                                                       <  5:638519/151‑1 (MQ=255)
                                                     aGCGGCGGCGAGGCCAGGCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATAc                                                                                                   <  8:1098072/151‑1 (MQ=255)
                                                      gcggcggcgAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACa                                                                                                  <  1:1818671/151‑1 (MQ=255)
                                                        ggcggcgAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAgg                                                                                                >  7:1272208/1‑151 (MQ=255)
                                                         gcggcgAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAggt                                                                                               <  2:169681/151‑1 (MQ=255)
                                                          cggcgAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAggtg                                                                                              >  7:268217/1‑151 (MQ=255)
                                                           ggcgAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAggtgg                                                                                             <  2:1174562/151‑1 (MQ=255)
                                                                  ccagccagTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAAcc                                                                                      <  4:1619673/151‑1 (MQ=255)
                                                                   cagccagTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCg                                                                                     <  4:212542/151‑1 (MQ=255)
                                                                   cagccagTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCg                                                                                     >  2:654380/1‑151 (MQ=255)
                                                                     gccagTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGt                                                                                    >  6:68474/1‑150 (MQ=255)
                                                                      ccagTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTAt                                                                                  <  2:1783203/151‑1 (MQ=255)
                                                                           tagttTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCcac                                                                             >  5:1022416/1‑151 (MQ=255)
                                                                            agttTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCcac                                                                             <  1:1211544/150‑1 (MQ=255)
                                                                            agttTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGGTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCcaca                                                                            <  4:508678/151‑1 (MQ=255)
                                                                                ttAAGCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCGTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAgg                                                                         <  8:1008470/150‑1 (MQ=255)
                                                                                ttAACCAGCGGATGGATACGGCCTGCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGt                                                                        >  4:1175067/1‑151 (MQ=255)
                                                                                 tAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGt                                                                        <  5:715567/150‑1 (MQ=255)
                                                                                  aaCCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGtt                                                                       <  2:1680185/150‑1 (MQ=255)
                                                                                   aCCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTaa                                                                     <  1:1017493/151‑1 (MQ=255)
                                                                                   aCCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTa                                                                      >  4:945153/1‑150 (MQ=255)
                                                                                      aGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCca                                                                              >  8:1635039/1‑139 (MQ=255)
                                                                                             ggatACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTtc                                                           <  6:106031/151‑1 (MQ=255)
                                                                                             ggatACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGtt                                                            <  1:1689883/150‑1 (MQ=255)
                                                                                                 aCGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTtcgtcg                                                       <  5:912358/151‑1 (MQ=255)
                                                                                                  ctgCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTtcgtcg                                                       >  3:1421795/3‑150 (MQ=255)
                                                                                                        tCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATa                                                  <  8:340491/149‑1 (MQ=255)
                                                                                                        tCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCCGGCAGCCGCCCAGAGTTACCAATACAGGTGGTACAAACGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATAcc                                                >  1:689437/1‑150 (MQ=255)
                                                                                                         cAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACgg                                               <  5:508190/151‑1 (MQ=255)
                                                                                                         cAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACgg                                               <  8:1929131/151‑1 (MQ=255)
                                                                                                         cAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACgg                                               >  1:348903/1‑151 (MQ=255)
                                                                                                          aaaGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGgt                                              <  6:1742098/151‑1 (MQ=255)
                                                                                                          aaaGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGgt                                              <  3:949952/151‑1 (MQ=255)
                                                                                                           aaGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTAGCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGgtg                                             >  3:1599375/1‑151 (MQ=255)
                                                                                                           aaGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGgtg                                             <  4:1302428/151‑1 (MQ=255)
                                                                                                            aGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGgtgt                                            <  8:51213/151‑1 (MQ=255)
                                                                                                             gTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTc                                           <  4:1434831/151‑1 (MQ=255)
                                                                                                             gTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTc                                           <  6:1638890/151‑1 (MQ=255)
                                                                                                                  ggTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGttt                                      <  7:313523/151‑1 (MQ=255)
                                                                                                                   gTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTc                                     <  4:344598/151‑1 (MQ=255)
                                                                                                                   gTTGCCGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTc                                     <  2:1237472/151‑1 (MQ=255)
                                                                                                                        cGGACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCttt                                >  1:1105631/1‑151 (MQ=255)
                                                                                                                         ggACAGCACCGCACCGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCtttt                               >  2:519933/1‑151 (MQ=255)
                                                                                                                         ggACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGCCCCAGCGTTACCAATACAGGTGGTACAACCGTATCCCACAACGTTACACACCAGTTCGTCGAGATAAGGTGTCAGTTTCGCgttt                               >  5:410378/1‑151 (MQ=255)
                                                                                                                              gcaccgcaccGACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAg                          <  4:1030977/151‑1 (MQ=255)
                                                                                                                              gcaccgcaccGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAg                          <  7:1302691/151‑1 (MQ=255)
                                                                                                                              ccaccgcaccgACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGGACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAg                          <  4:761943/150‑1 (MQ=255)
                                                                                                                                accgcaccgACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATCCGGTGTCAGTTTCGCTTTTGcc                            >  3:1080161/1‑147 (MQ=255)
                                                                                                                                accgcaccgACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGcc                            >  2:893615/1‑147 (MQ=255)
                                                                                                                                accgcaccgACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGAt                        <  7:1410205/151‑1 (MQ=255)
                                                                                                                                 ccgcaccgACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGATa                       <  3:795873/151‑1 (MQ=255)
                                                                                                                                  cgcaccgACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGATaa                      <  5:147026/151‑1 (MQ=255)
                                                                                                                                   gcaccgACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGATAAt                     >  7:541140/1‑151 (MQ=255)
                                                                                                                                     accgACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTTCAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGACAGATAATc                    >  5:1334789/1‑150 (MQ=255)
                                                                                                                                        gACGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGATAATCAGa                 >  5:1749900/1‑150 (MQ=255)
                                                                                                                                         gcGGTTAAATCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTGTTTCCAGATAATCAGaa                >  3:687887/2‑150 (MQ=255)
                                                                                                                                          cGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGATAATCAGAAAc              >  3:331930/1‑151 (MQ=255)
                                                                                                                                                aaTCGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGATAATCAGAAACGACttt        <  5:1831168/151‑1 (MQ=255)
                                                                                                                                                aaTCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGATAATCAGAAACGACttt        <  6:529358/151‑1 (MQ=255)
                                                                                                                                                 aTCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGATAATCAGAAACGACTTTc       <  4:1662483/151‑1 (MQ=255)
                                                                                                                                                   cGCTTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGATAATCAGAAACGACTTTCg      <  5:248552/150‑1 (MQ=255)
                                                                                                                                                    gCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGATAATCAGAAACGACTTTCGa     >  1:1326781/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GCTCAGAAGCCAGGTTGATATTCATATTTCCCGCCAGCGCATAGGCAACCACCAGCGGCGGCGAGGCCAGCCAGTTAGTTTTAACCAGCGGATGGATACGGCCTTCAAAGTTACGGTTGCCGGACAGCACCGCACCGACGGTTAAATCGCCTTTTTTGATTGCCGTTTCGATAGGATCGGGCAGCGGCCCAGAGTTACCAATACAGGTGGTACAACCGTATCCCACAAGGTTAAACCCCAGTTCGTCGAGATACGGTGTCAGTTTCGCTTTTGCCAGATAATCAGAAACGACTTTCGAACC  >  DH1/2542133‑2542433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑