Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,480,8680TC55.3% 204.5 / inf 563I21T (ATA→ACA) ECDH1_RS17240hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (129/122);  new base C (162/148);  total (292/271)
Fisher's exact test for biased strand distribution p-value = 8.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-02

Only 100 of 571 total aligned reads displayed.

TCAGGGACGTAATAAGTTGATCCATCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGTTTTAATTATTCAATTTATAGCAGCGC  >  DH1/3480718‑3481018
                                                                                                                                                      |                                                                                                                                                      
 cAGGGACGTAATAAGTTGATCCATCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAAt                                                                                                                                                        <  6:1042741/150‑1 (MQ=255)
 cAGGGACGTAATAAGTTGATCCATCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATa                                                                                                                                                       <  4:775515/151‑1 (MQ=255)
    ggACGTAATAAGTTGATCCATCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGc                                                                                                                                                    <  1:1857240/151‑1 (MQ=255)
        gTAATAAGTTGATCCATCAATTATCAATTCATAGAAATCATTTTATGTTTCGCTCTAACGATTATGCTGCGTTACAGACGAATGGTTGAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCa                                                                                                                                                <  7:1396656/151‑1 (MQ=255)
            taaGTTGATCCATCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGAATCAAAtt                                                                                                                                            >  6:309589/1‑151 (MQ=255)
             aaGTTGATCCATCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAAtt                                                                                                                                            <  8:1587738/150‑1 (MQ=255)
              aGTTGATCCATCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCa                                                                                                                                          <  3:1020497/151‑1 (MQ=255)
                  gATCCATCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATaa                                                                                                                                       <  7:220532/150‑1 (MQ=255)
                    tCCATCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTCGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATaaa                                                                                                                                      >  2:1344876/1‑149 (MQ=255)
                     ccATCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGc                                                                                                                                   <  4:1428824/151‑1 (MQ=255)
                        tCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGcac                                                                                                                                 >  8:829940/1‑150 (MQ=255)
                        tCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGcac                                                                                                                                 >  5:795155/1‑150 (MQ=255)
                         cAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCTCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGcac                                                                                                                                 >  7:1593000/1‑149 (MQ=255)
                          aaTTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACCCATCAAATTCATAAAAGCACAGt                                                                                                                              >  2:87204/1‑151 (MQ=255)
                          aaTTATCAATGCATACAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGt                                                                                                                              <  7:1767203/151‑1 (MQ=255)
                             tATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTAtt                                                                                                                           >  8:1903173/1‑151 (MQ=255)
                               tCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCAAAGTAttt                                                                                                                          >  4:1703548/1‑150 (MQ=255)
                                cgaTGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTc                                                                                                                         >  4:1323632/3‑150 (MQ=255)
                                cAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCa                                                                                                                        <  3:75589/151‑1 (MQ=255)
                                 aaTGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCa                                                                                                                        >  1:546951/1‑150 (MQ=255)
                                 aaTGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCa                                                                                                                        >  1:1173417/1‑150 (MQ=255)
                                  aTGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAAc                                                                                                                      <  6:1918777/151‑1 (MQ=255)
                                  aTGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAAc                                                                                                                      >  5:1315943/1‑151 (MQ=255)
                                     cATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATg                                                                                                                   >  2:1859174/1‑151 (MQ=255)
                                        aGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGtt                                                                                                                 <  5:1704284/150‑1 (MQ=255)
                                        aGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTg                                                                                                                <  4:1736964/151‑1 (MQ=255)
                                        aGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTg                                                                                                                <  3:88630/151‑1 (MQ=255)
                                         gAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGc                                                                                                               <  5:1073793/151‑1 (MQ=255)
                                          aaaaCATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGc                                                                                                               >  3:1399644/1‑150 (MQ=255)
                                            aaCATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGa                                                                                                            <  1:112287/151‑1 (MQ=255)
                                              cATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGAt                                                                                                           >  1:971381/1‑150 (MQ=255)
                                                 attatGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAAtt                                                                                                       <  5:1538108/151‑1 (MQ=255)
                                                  ttatGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAAttt                                                                                                      >  2:1725678/1‑151 (MQ=255)
                                                     tGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGt                                                                                                   >  7:1086771/1‑151 (MQ=255)
                                                      gTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGtt                                                                                                  <  1:1731572/151‑1 (MQ=255)
                                                       tttCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGtt                                                                                                  <  2:1562928/150‑1 (MQ=255)
                                                        ttCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGtttt                                                                                                >  5:1017756/1‑151 (MQ=255)
                                                            cgcTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGa                                                                                             >  3:563358/1‑150 (MQ=255)
                                                              cTAACGATTATGCTGCGTTACAGACGAATGGTCCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGc                                                                                          >  7:404419/1‑151 (MQ=255)
                                                               tAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGc                                                                                          >  5:570955/1‑150 (MQ=255)
                                                               tAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTAATAAAATCACAGTATTACAACATGTTGCAGATAATTTTGTTATTGAAGc                                                                                          >  3:1707280/1‑150 (MQ=255)
                                                               tAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAAATCATAAAAGAACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAga                                                                                          >  2:285035/1‑149 (MQ=255)
                                                                aaCGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCAt                                                                                        >  2:423698/1‑151 (MQ=255)
                                                                aaCGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCAt                                                                                        >  3:1201840/1‑151 (MQ=255)
                                                                 aCGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATa                                                                                       <  6:987418/151‑1 (MQ=255)
                                                                 aCGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATa                                                                                       <  5:1896635/151‑1 (MQ=255)
                                                                  cGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATAc                                                                                      >  2:7534/1‑151 (MQ=255)
                                                                  cGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATAc                                                                                      <  6:624726/151‑1 (MQ=255)
                                                                  cGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATAc                                                                                      >  7:930011/1‑151 (MQ=255)
                                                                       aTGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCat                                                                                  <  6:369228/150‑1 (MQ=255)
                                                                       aTGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCat                                                                                  <  4:990475/150‑1 (MQ=255)
                                                                       aTGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCat                                                                                  >  1:27917/1‑150 (MQ=255)
                                                                          ctgcGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCataat                                                                               >  7:669310/1‑150 (MQ=255)
                                                                              gTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATaataaata                                                                          <  1:1261392/151‑1 (MQ=255)
                                                                                 aCAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATca                                                                       <  4:111568/151‑1 (MQ=255)
                                                                                  cAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATcac                                                                      <  7:815355/151‑1 (MQ=255)
                                                                                   aGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATcac                                                                      >  1:89102/1‑150 (MQ=255)
                                                                                    gACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATcacaa                                                                    <  6:74882/151‑1 (MQ=255)
                                                                                    gACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATcaca                                                                     >  4:1595161/1‑150 (MQ=255)
                                                                                    gACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATcaca                                                                     >  8:1673395/1‑150 (MQ=255)
                                                                                     aCGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATcacaaa                                                                   <  8:459980/151‑1 (MQ=255)
                                                                                     aCGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATcacaaa                                                                   <  8:1508340/151‑1 (MQ=255)
                                                                                       gAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATcacaaaca                                                                 <  7:1328734/151‑1 (MQ=255)
                                                                                               cAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAAcc                                                           >  1:1325260/1‑149 (MQ=255)
                                                                                               cAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAg                                                         <  1:720279/151‑1 (MQ=255)
                                                                                                aaTTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAAcc                                                           >  8:1803069/1‑148 (MQ=255)
                                                                                                   tattCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATa                                                      >  5:632224/1‑150 (MQ=255)
                                                                                                      tCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAat                                                   >  5:360538/1‑150 (MQ=255)
                                                                                                         ttCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATcc                                               >  4:352092/1‑151 (MQ=255)
                                                                                                         ttCCACTAAGGCCCAATGTGGACATATATAATTTCTGGCGAAACACACGCATGAAATTCAAAACAGCACACTATATCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACCAACACAAACCAGATAAATATc                                                <  8:304929/150‑1 (MQ=255)
                                                                                                            cccTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTAt                                            >  5:860039/1‑151 (MQ=255)
                                                                                                             ccTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATg                                           >  8:1124123/1‑151 (MQ=255)
                                                                                                              cTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATg                                           >  3:480625/1‑150 (MQ=255)
                                                                                                                aaGTCCGAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGa                                          <  4:643942/149‑1 (MQ=255)
                                                                                                                aaGTCCGAATGTGGACATATATAATTTATGGAGAAAAACAAGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTTTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGaa                                         <  8:1038541/150‑1 (MQ=255)
                                                                                                                 aGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAata                                       <  6:500865/151‑1 (MQ=255)
                                                                                                                 aGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAata                                       <  6:1155004/151‑1 (MQ=255)
                                                                                                                    ccGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATg                                     <  1:1418315/150‑1 (MQ=255)
                                                                                                                     cGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGc                                    >  8:551324/1‑150 (MQ=255)
                                                                                                                      gAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGca                                   >  8:1209934/1‑150 (MQ=255)
                                                                                                                      gAATGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTAGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGca                                   >  6:1482531/1‑150 (MQ=255)
                                                                                                                       aaTGTGGTCATATATAATTTATGGAGAAAAATACTCATCAAATTCATAAAATCAGAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGcaa                                  <  8:655541/150‑1 (MQ=255)
                                                                                                                       aaTGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGcaac                                 >  5:1222797/1‑151 (MQ=255)
                                                                                                                       aaTGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGcaa                                  >  1:404078/1‑150 (MQ=255)
                                                                                                                        aTGTGGACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGcaac                                 >  1:1655821/1‑150 (MQ=255)
                                                                                                                            ggACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAg                             <  3:1512433/150‑1 (MQ=255)
                                                                                                                            ggACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAg                             <  7:604680/150‑1 (MQ=255)
                                                                                                                            ggACATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGt                            >  4:455206/1‑151 (MQ=255)
                                                                                                                              aCATATATAATTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGttt                          >  1:1031644/1‑151 (MQ=255)
                                                                                                                               cATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGtttt                         >  5:1482351/1‑151 (MQ=255)
                                                                                                                                  atataATTTATGGAGAAAAACACGCATCAAAATCATAAAAGCACAGTATTTCAACATGTGGCAGATAATTTTGTTTTTGAAGCATACGCAGAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGGTTTAat                      >  4:264799/1‑151 (MQ=255)
                                                                                                                                      aaTTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGTTTTAattatt                  <  3:221289/151‑1 (MQ=255)
                                                                                                                                       aTTTATGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGTTTTAATTATTc                 <  7:1670408/151‑1 (MQ=255)
                                                                                                                                         ttATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGTTTTAATTATTCa                <  4:1364322/150‑1 (MQ=255)
                                                                                                                                         ttATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGATTTAATTATTCa                >  3:972971/1‑150 (MQ=255)
                                                                                                                                           aTGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGTTTTAATTATTCAAtt             <  6:1808153/151‑1 (MQ=255)
                                                                                                                                            tGGAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGTTTTAATTATTCAAttt            >  6:1560331/1‑151 (MQ=255)
                                                                                                                                             ggAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGTTTTAATTATTCAATTta           <  2:685647/151‑1 (MQ=255)
                                                                                                                                             ggAGAAAAACACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGTTTTAATTATTCAAttt            <  2:1153384/150‑1 (MQ=255)
                                                                                                                                                 aaaaaTACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGTTTTAATTATTCAATTTATagc       <  8:1349200/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
TCAGGGACGTAATAAGTTGATCCATCAATTATCAATGCATAGAAAACATATTATGTTTCGCGCTAACGATTATGCTGCGTTACAGACGAATGGTTCAATTATTCTTTCCCCTAAGTCCGAATGTGGACATATATAATTTATGGAGAAAAATACGCATCAAATTCATAAAAGCACAGTATTTCAACATGTTGCAGATAATTTTGTTTTTGAAGCATACGCATAATAAATATCACAAACACAAACCAGATAAATATCCTATGAATATGCAACAAAGTTTTAATTATTCAATTTATAGCAGCGC  >  DH1/3480718‑3481018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑