Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,141,1600AG53.1% 105.3 / inf 457T272A (ACG→GCG) yjeJhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (113/100);  new base G (112/129);  total (226/231)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 483 total aligned reads displayed.

ACGCCGGGTAAAGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTAAACGCCTTAT  >  DH1/4141010‑4141310
                                                                                                                                                      |                                                                                                                                                      
aCGCCGGGTATAGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAACCTCGGTGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCGCAACCGCTAACCCAGGATCAATTCTTacgca                                                                                                                                                        <  2:1037977/151‑1 (MQ=255)
 cGCCGGGTAAAGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTacgcac                                                                                                                                                       >  2:1286853/1‑151 (MQ=255)
 cGCCGGGTAAAGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTacgcac                                                                                                                                                       <  2:483253/151‑1 (MQ=255)
   ccGGGTAAAGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTacgcacgc                                                                                                                                                     >  5:133779/1‑151 (MQ=255)
   ccGGGTAAAGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTacgcacgc                                                                                                                                                     <  7:325372/151‑1 (MQ=255)
     gggTAAAGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTg                                                                                                                                                   <  7:1134657/151‑1 (MQ=255)
     gggTAAAGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTg                                                                                                                                                   <  8:438612/151‑1 (MQ=255)
       gTAAAGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGc                                                                                                                                                  >  1:613791/1‑150 (MQ=255)
         aaaGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCAt                                                                                                                                                <  4:1109251/150‑1 (MQ=255)
          aaGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATc                                                                                                                                               >  3:1381496/1‑150 (MQ=255)
           aGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCAc                                                                                                                                             >  4:1301691/1‑151 (MQ=255)
                 aGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACgt                                                                                                                                       >  6:1725127/1‑151 (MQ=255)
                  gAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACgtg                                                                                                                                      <  8:160193/151‑1 (MQ=255)
                      cGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCCTCACTTACGTGTTCa                                                                                                                                  <  6:241957/151‑1 (MQ=255)
                      cGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCa                                                                                                                                  <  6:394839/151‑1 (MQ=255)
                      cGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTc                                                                                                                                   >  1:726256/1‑150 (MQ=255)
                      cGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCAGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGGTTACGCACGCTGCATCACTTACGTGTTCa                                                                                                                                  <  1:1127479/151‑1 (MQ=255)
                      cGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTAGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTc                                                                                                                                   >  7:1411694/1‑150 (MQ=255)
                       gATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCaa                                                                                                                                 <  8:557727/151‑1 (MQ=255)
                        aTCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAAt                                                                                                                                <  8:1927569/151‑1 (MQ=255)
                           aCTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAAACCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATcc                                                                                                                              >  6:1824535/1‑150 (MQ=255)
                               gCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATccacc                                                                                                                           >  3:1852359/1‑149 (MQ=255)
                                 cGAATGCTTGATTTCAGCCCACGACTGAAAAATCTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTTTTCAATCCACCAGCa                                                                                                                       <  3:1096038/151‑1 (MQ=255)
                                 cGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCCGCATCACTTACGTGTTCAATCCACCAGCa                                                                                                                       <  5:614959/151‑1 (MQ=255)
                                 cGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCa                                                                                                                       <  8:111749/151‑1 (MQ=255)
                                   aaTGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCaa                                                                                                                      >  7:979935/1‑150 (MQ=255)
                                    aTGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCaaaa                                                                                                                    <  7:1684334/151‑1 (MQ=255)
                                    aTGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCaaa                                                                                                                     >  3:1413064/1‑150 (MQ=255)
                                     tGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAAc                                                                                                                   >  6:495746/1‑151 (MQ=255)
                                      gCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACt                                                                                                                  <  8:695217/151‑1 (MQ=255)
                                         tGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGcc                                                                                                               >  7:1842304/1‑150 (MQ=255)
                                          gATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGcac                                                                                                              >  2:1222788/1‑151 (MQ=255)
                                          gATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGca                                                                                                               <  6:1444531/150‑1 (MQ=255)
                                          gATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGca                                                                                                               <  6:94094/150‑1 (MQ=255)
                                          gATTTCAGCCCACGACTCAAAAAACTCGCCGGTGTGCCATCTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCCCTAACCCAGGATCAATCCTTACGCACGCTCCATCACATACCTGATCAATCCACCAGCAAAACTCcac                                                                                                              >  2:684731/1‑151 (MQ=255)
                                             ttCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTTCGTGTTCACTCCACCAGCAAAACTGcaccac                                                                                                           >  2:998729/1‑151 (MQ=255)
                                               cAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACcaca                                                                                                          <  7:641849/150‑1 (MQ=255)
                                                aGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAgg                                                                                                        >  2:627201/1‑151 (MQ=255)
                                                   ccACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCg                                                                                                      >  6:1195121/1‑150 (MQ=255)
                                                     aCGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGaa                                                                                                    >  8:1395305/1‑150 (MQ=255)
                                                     aCGACTGAAAAAACTCGCGGGTGTGCCAGGTCAGGGCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGaaa                                                                                                   <  8:232928/151‑1 (MQ=255)
                                                        aCTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAaataa                                                                                                <  1:490011/151‑1 (MQ=255)
                                                        aCTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAaata                                                                                                 >  4:72223/1‑150 (MQ=255)
                                                           gAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAAtct                                                                                             >  8:127769/1‑151 (MQ=255)
                                                            aaaaaaCTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAAtctc                                                                                            <  1:1451903/151‑1 (MQ=255)
                                                               aaaCTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATc                                                                                         >  1:1702894/1‑151 (MQ=255)
                                                                aaCTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCt                                                                                        <  1:345868/151‑1 (MQ=255)
                                                                 aCTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCtt                                                                                       >  4:400227/1‑151 (MQ=255)
                                                                    cgcgGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAACCTGCACCACAGGCGAAATAATCTCATCttt                                                                                      <  2:249179/149‑1 (MQ=255)
                                                                    cgcgGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGc                                                                                    <  1:1603829/151‑1 (MQ=255)
                                                                     gcgGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCt                                                                                   <  6:756585/151‑1 (MQ=255)
                                                                     gcgGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCt                                                                                   >  4:916360/1‑151 (MQ=255)
                                                                       gggTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTaa                                                                                 <  4:251666/151‑1 (MQ=255)
                                                                           gtgCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCg                                                                             >  4:761304/1‑151 (MQ=255)
                                                                             gCCATGTCAGGTCTATGTTCGGACCGTAGCCGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCg                                                                             >  1:1020427/1‑149 (MQ=255)
                                                                                  gTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTgg                                                                      >  6:1013284/1‑151 (MQ=255)
                                                                                    cAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGa                                                                     <  2:1704224/150‑1 (MQ=255)
                                                                                    cAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGa                                                                     <  5:381397/150‑1 (MQ=255)
                                                                                      ggTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGcc                                                                  >  3:1608116/1‑151 (MQ=255)
                                                                                       gTCTATGTTCGCAGCGTAGCGGCGAATAACGCCGAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATGACTTACGTGTTCAATCCACCAGCAAAACTGGACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCa                                                                 <  1:239086/151‑1 (MQ=255)
                                                                                        tCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCAt                                                                >  4:143364/1‑151 (MQ=255)
                                                                                         cTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATc                                                               >  4:1738433/1‑151 (MQ=255)
                                                                                           aTGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATcc                                                              <  8:1879197/150‑1 (MQ=255)
                                                                                           aTGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCa                                                             <  2:1413216/151‑1 (MQ=255)
                                                                                           aTGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATcc                                                              >  4:119841/1‑150 (MQ=255)
                                                                                           aTGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATcc                                                              >  3:433624/1‑150 (MQ=255)
                                                                                                cGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTg                                                        <  3:997431/151‑1 (MQ=255)
                                                                                                       tAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGAc                                                 >  6:1566692/1‑151 (MQ=255)
                                                                                                        aGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACt                                                <  4:337683/151‑1 (MQ=255)
                                                                                                          cggcgAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTg                                               >  5:1480659/1‑150 (MQ=255)
                                                                                                             cgAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATaa                                           <  5:1829583/151‑1 (MQ=255)
                                                                                                             cgAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATa                                            >  8:887036/1‑150 (MQ=255)
                                                                                                              gAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAAc                                          >  4:855837/1‑151 (MQ=255)
                                                                                                                  aaCGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGa                                      <  3:1303232/151‑1 (MQ=255)
                                                                                                                   aCGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGAt                                     <  1:1772068/151‑1 (MQ=255)
                                                                                                                    cGCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATc                                    >  2:934415/1‑151 (MQ=255)
                                                                                                                     gCCCAACCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACACGCGAAATAAACTCATCTTTGCTAATGCGACGCTCGACCCATCCACCCTGAAATGACTGATAACGTGATcc                                   >  5:812240/1‑150 (MQ=255)
                                                                                                                        cAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTg                                <  5:483994/151‑1 (MQ=255)
                                                                                                                         aaCCGCTAACCCAGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTgg                               >  7:870708/1‑151 (MQ=255)
                                                                                                                           ccGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTc                             <  4:571721/151‑1 (MQ=255)
                                                                                                                            cGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTc                             >  4:1583103/1‑150 (MQ=255)
                                                                                                                                aaCCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGcc                        <  6:1152769/151‑1 (MQ=255)
                                                                                                                                   ccAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCgg                      >  3:70401/1‑150 (MQ=255)
                                                                                                                                     aGGATCAATGCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATg                   >  5:743915/1‑151 (MQ=255)
                                                                                                                                      ggATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATgc                  <  7:1284482/151‑1 (MQ=255)
                                                                                                                                       gATCAATGCTTACGCACGCTGCATCTCTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATgcg                 <  3:803194/151‑1 (MQ=255)
                                                                                                                                       gATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATgcg                 >  7:1573965/1‑151 (MQ=255)
                                                                                                                                        aTCAATGCTTACGCACTCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATgcgg                <  7:1926215/151‑1 (MQ=255)
                                                                                                                                            aTGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCGGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTa            <  2:413291/151‑1 (MQ=255)
                                                                                                                                              gCTTACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTaaa          <  6:580305/151‑1 (MQ=255)
                                                                                                                                              gCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTaa           >  7:467902/1‑150 (MQ=255)
                                                                                                                                              gCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTaa           >  3:289038/1‑150 (MQ=255)
                                                                                                                                                 tACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTAAAc         <  1:1338406/149‑1 (MQ=255)
                                                                                                                                                 tACGCGCGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTAAACGc       >  5:321586/1‑151 (MQ=255)
                                                                                                                                                  acgcgcgcTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTAAACGc       <  5:1629865/150‑1 (MQ=255)
                                                                                                                                                  acgcacgcTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTAAACGcc      <  7:1035376/151‑1 (MQ=255)
                                                                                                                                                   cgcgcgcTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTAAACGCCt     >  1:1177349/1‑151 (MQ=255)
                                                                                                                                                    gcacgcTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTAAACGCCtt    <  2:1519280/151‑1 (MQ=255)
                                                                                                                                                     cacgcTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCAATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTAAACGCCtt    >  2:1571536/1‑150 (MQ=255)
                                                                                                                                                      acgcTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTAAACGCCTTAt  >  5:1158667/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
ACGCCGGGTAAAGAAATAGAAGCGATCACTCGCCGAATGCTTGATTTCAGCCCACGACTGAAAAAACTCGCGGGTGTGCCATGTCAGGTCTATGTTCGCACCGTAGCGGCGAATAACGCCCAACCGCTAACCCAGGATCAATGCTTACGCACGCTGCATCACTTACGTGTTCAATCCACCAGCAAAACTGCACCACAGGCGAAATAATCTCATCTTTGCTAATGCGAGGCTGGAGCCATCCAGCCTGAAATGACTGATAACGTGATCGTTGGTCATGCCGGATGCGGCGTAAACGCCTTAT  >  DH1/4141010‑4141310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑