Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH14,547,8150TC54.3% 135.0 / inf 464Y80C (TAC→TGC) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base T (111/100);  new base C (129/122);  total (241/223)
Fisher's exact test for biased strand distribution p-value = 8.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

Only 100 of 466 total aligned reads displayed.

TGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTT  >  DH1/4547666‑4547963
                                                                                                                                                     |                                                                                                                                                     
tgttgCGGGGGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTCCCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTTGGGGAAGCGtt                                                                                                                                                      >  2:971363/1‑150 (MQ=255)
 gttgCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTa                                                                                                                                                      <  3:595024/150‑1 (MQ=255)
   tgcgtgcgTTTTCAGGTTTGTCGAAGTTATCTTCGTTAACTTTCAGCTGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGa                                                                                                                                                    <  2:1289751/150‑1 (MQ=255)
   tgCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGa                                                                                                                                                    <  5:1568824/150‑1 (MQ=255)
    gCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGAt                                                                                                                                                   <  6:1430157/150‑1 (MQ=255)
    gCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGAt                                                                                                                                                   <  2:114104/150‑1 (MQ=255)
     cGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGt                                                                                                                                                 <  7:486409/151‑1 (MQ=255)
       ggCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGtca                                                                                                                                               <  6:1729876/151‑1 (MQ=255)
          gTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGtcatca                                                                                                                                            <  3:324775/151‑1 (MQ=255)
            tttCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAgg                                                                                                                                          >  2:835313/1‑151 (MQ=255)
            tttCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAgg                                                                                                                                          >  2:883804/1‑151 (MQ=255)
             ttCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAgg                                                                                                                                          >  8:753163/1‑150 (MQ=255)
             ttCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAACGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAgg                                                                                                                                          <  2:602064/150‑1 (MQ=255)
              tCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTCCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGAc                                                                                                                                        >  6:12592/1‑151 (MQ=255)
               cAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGAcc                                                                                                                                       >  6:795257/1‑151 (MQ=255)
                aGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCg                                                                                                                                      >  7:634397/1‑151 (MQ=255)
                aGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCg                                                                                                                                      <  2:707363/151‑1 (MQ=255)
                 ggTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCgg                                                                                                                                     <  7:1574377/151‑1 (MQ=255)
                 ggTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCgg                                                                                                                                     >  1:1146152/1‑151 (MQ=255)
                   tttGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGAcc                                                                                                                                       <  5:748808/147‑1 (MQ=255)
                    ttGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGCCCGGCg                                                                                                                                   >  3:1447029/1‑150 (MQ=255)
                    ttGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCg                                                                                                                                   <  4:519180/150‑1 (MQ=255)
                      gTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAgg                                                                                                                                >  4:1578549/1‑151 (MQ=255)
                      gTCGAAGGTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAgg                                                                                                                                <  3:688857/151‑1 (MQ=255)
                        cGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGt                                                                                                                               <  6:98922/150‑1 (MQ=255)
                           aGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAgg                                                                                                                            >  2:370271/1‑150 (MQ=255)
                            gTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAgg                                                                                                                            >  4:1053116/1‑149 (MQ=255)
                               aaCTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTg                                                                                                                       >  6:1302923/1‑151 (MQ=255)
                                   tCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCt                                                                                                                    <  6:583414/150‑1 (MQ=255)
                                   tCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCt                                                                                                                    <  4:91153/150‑1 (MQ=255)
                                    cGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTg                                                                                                                   <  5:177479/150‑1 (MQ=255)
                                      ttAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCgg                                                                                                                >  1:702675/1‑151 (MQ=255)
                                         aCTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAAc                                                                                                             <  4:1301243/151‑1 (MQ=255)
                                           tttCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGACACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACgg                                                                                                           >  6:90505/1‑151 (MQ=255)
                                           tttCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACgg                                                                                                           >  4:627140/1‑151 (MQ=255)
                                              cagcagCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACggagg                                                                                                        <  5:1358104/151‑1 (MQ=255)
                                              cagcagCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGCCCGGCGAGGTAGGAGCTGTCTGCGGAACggagg                                                                                                        >  5:1110771/1‑151 (MQ=255)
                                              cagcagCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACggag                                                                                                         <  7:758522/150‑1 (MQ=255)
                                               agcagcGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACggagg                                                                                                        >  2:1858139/1‑150 (MQ=255)
                                                gcagcGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACggagga                                                                                                       >  6:1076778/1‑150 (MQ=255)
                                                 cagcGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGaaa                                                                                                     <  3:1364261/151‑1 (MQ=255)
                                                     ccAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCaa                                                                                                 <  3:199208/150‑1 (MQ=255)
                                                      cAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGCTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTGATCAGGCCCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAACCCaa                                                                                                 >  7:116525/1‑150 (MQ=255)
                                                        aaaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGACACGCAGATGTCATCAGGCCCGGCGAGGGAGGAGCTGTCTGCGGAACGGAGGAAACCAActc                                                                                              >  6:403948/1‑151 (MQ=255)
                                                        aaaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatc                                                                                              >  1:812034/1‑151 (MQ=255)
                                                        aaaTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatc                                                                                              >  7:135847/1‑151 (MQ=255)
                                                         aaTAGCGTTCACCTTCTTTGGGCGGGCGAATCTTAGCAGAGATGGTATCACCAGTGCTGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCTGAACGGAGGAAACCAAatca                                                                                             <  3:1137499/151‑2 (MQ=255)
                                                          aTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAACCCAAatcc                                                                                             >  1:961066/1‑150 (MQ=255)
                                                          aTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatcc                                                                                             >  1:1581364/1‑150 (MQ=255)
                                                          aTAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAatcca                                                                                            <  5:96743/151‑1 (MQ=255)
                                                                  cACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCaa                                                                                    <  4:187654/151‑1 (MQ=255)
                                                                       tcttTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAt‑c                                                                               <  1:1416465/150‑1 (MQ=255)
                                                                         tttCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTcc                                                                            >  5:543428/1‑151 (MQ=255)
                                                                          ttCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATGCTGCAATAt‑cggc                                                                            >  6:1897728/1‑147 (MQ=255)
                                                                          ttCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAt‑ct                                                                              <  3:1864430/148‑1 (MQ=255)
                                                                            cggcggGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGt                                                                         >  8:638271/1‑151 (MQ=255)
                                                                             ggcgggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGt                                                                         >  7:1546177/1‑150 (MQ=255)
                                                                              gcgggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTAc                                                                       >  1:1026256/1‑151 (MQ=255)
                                                                                gggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGc                                                                     >  8:1449911/1‑151 (MQ=255)
                                                                                 ggcgAATCTTACCAGAGATGGTATGAGCAGTGCGGAGGTTGTAACGGAGGATTTGGCTAGGGGAAAGGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGGAATAT‑CTCCAGTACGcc                                                                    <  4:1232189/151‑1 (MQ=255)
                                                                                 ggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGcc                                                                    <  5:344801/151‑1 (MQ=255)
                                                                                 ggcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGcc                                                                    >  2:294498/1‑151 (MQ=255)
                                                                                  gcgAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCa                                                                   <  5:124738/151‑1 (MQ=255)
                                                                                    gAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGGTGTCTGCGGAACGGAGGAACCCAAAACCATCCTGCAATAT‑CTCCAGTACGCCATc                                                                 >  8:1757672/1‑151 (MQ=255)
                                                                                     aaTCTTACCAGATATGGTATGACCATTGCGGAGGTTGAAACGGCGGATTTGGGTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCa                                                                <  2:1814488/151‑1 (MQ=255)
                                                                                     aaTCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATc                                                                 >  5:1712240/1‑150 (MQ=255)
                                                                                           aCCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCaa                                                            >  7:958685/1‑149 (MQ=255)
                                                                                             cAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGat                                                        <  8:1883082/151‑1 (MQ=255)
                                                                                              agagATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGata                                                       <  8:258640/151‑1 (MQ=255)
                                                                                              agagATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGata                                                       <  8:412792/151‑1 (MQ=255)
                                                                                               gagaTGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGatat                                                      <  3:1653119/151‑1 (MQ=255)
                                                                                                    ggTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCg                                                 <  6:1633266/151‑1 (MQ=255)
                                                                                                     gTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGc                                                <  5:1867315/151‑1 (MQ=255)
                                                                                                         cACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAACCGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTTTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑ATCCAGTACGCCATCACCAAAGATATCTTCGCCAct                                            <  8:556602/151‑1 (MQ=255)
                                                                                                         cACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCAct                                            >  4:1353169/1‑151 (MQ=255)
                                                                                                         cACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCAct                                            >  2:1393106/1‑151 (MQ=255)
                                                                                                          aCCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCActc                                           >  8:1612254/1‑151 (MQ=255)
                                                                                                            cAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCtt                                         >  5:251832/1‑151 (MQ=255)
                                                                                                             aGTGCGGAGGTTGAAACGGCGGA‑TTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTg                                       >  3:828538/1‑151 (MQ=255)
                                                                                                               ggCGGAGGCGGACCCGGCGGAATTCGCGAGGGGACACGCAGATGTCAGCACGACCGCCGAGGTAGGAGCTCGCTGCGGAACGGAGGACACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATACCTCCGCCACTCTTTGc                                      <  8:1218588/150‑1 (MQ=255)
                                                                                                                  ggAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtg                                   >  1:234612/1‑151 (MQ=255)
                                                                                                                  ggAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGGGGTAGGAGATGTCTGCGGACCGGAGGAACCCAAAGCAATCCT‑CAAAATACTCCAGTACCCCAGCACCAAAGATAAGTACGCCCGTCtttcggtg                                   >  4:1677152/1‑146 (MQ=255)
                                                                                                                  ggAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtg                                   <  5:841485/151‑1 (MQ=255)
                                                                                                                   gAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtgc                                  >  1:1759969/1‑151 (MQ=255)
                                                                                                                      gTTGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGtgctgc                               >  4:562758/1‑151 (MQ=255)
                                                                                                                        tGAAACGGCGGATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCtt                             >  2:437342/1‑151 (MQ=255)
                                                                                                                         gAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTc                            <  8:366822/151‑1 (MQ=255)
                                                                                                                          aaaCGGCGGATTTGGCTAGGGGAAACGCAGATATCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTc                            >  7:1765414/1‑150 (MQ=255)
                                                                                                                             cggcggATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAgg                         >  4:801901/1‑150 (MQ=255)
                                                                                                                              ggcggATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGAt                       <  7:1402265/151‑1 (MQ=255)
                                                                                                                              ggcggATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGAt                       <  3:467573/151‑1 (MQ=255)
                                                                                                                              ggcggATTTGGCTAGGGGAAACGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGAt                       <  7:29953/151‑1 (MQ=255)
                                                                                                                                      tGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaaaa                >  2:1660153/1‑150 (MQ=255)
                                                                                                                                       ggCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCaaaaa               <  1:639903/150‑1 (MQ=255)
                                                                                                                                       ggCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaat              >  2:1479105/1‑151 (MQ=255)
                                                                                                                                           aGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAaataa            <  5:1883655/149‑1 (MQ=255)
                                                                                                                                             gggAAACGCAGATGTCATCAGGACCGTCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTc        <  1:406589/151‑1 (MQ=255)
                                                                                                                                                aaaCGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTg     <  4:346109/151‑1 (MQ=255)
                                                                                                                                                aaaCGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTg     >  1:125440/1‑151 (MQ=255)
                                                                                                                                                  aCGCAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCt   <  3:1790081/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTTGCGGGCGTTTTCAGGTTTGTCGAAGTTAACTTCGTTAACTTTCAGCAGCGCAAAATAGCGTTCACCTTCTTTCGGCGGGCGAATCTTACCAGAGATGGTATCACCAGTGCGGAGGTTGAAACGGCGGATTTGGCTAGGGGAAACGTAGATGTCATCAGGACCGGCGAGGTAGGAGCTGTCTGCGGAACGGAGGAAACCAAATCCATCCTGCAATAT‑CTCCAGTACGCCATCACCAAAGATATCTTCGCCACTCTTTGCGTGCTGCTTCAGGATGGCAAAAATAATGTCCTGCTT  >  DH1/4547666‑4547963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑